Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope(SR-SIM).However,the imperfect optical...Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope(SR-SIM).However,the imperfect optical components can depolarize the laser beams hence degenerating the modulation depth.Here,we first presented a direct measurement method designed to estimate the modulation depth more precisely by shifting illumination patterns with equal phase steps.This measurement method greatly reduces the dependence of modulation depths on the samples,and then developed a polarization optimization method to achieve high modulation depth at all orientations by actively and quantitatively compensating for the additional phase difference using a combination of waveplate and a liquid crystal variable retarder(LCVR).Experimental results demonstrate that our method can achieve illumination patterns with modulation depth higher than 0.94 at three orientations with only one LCVR voltage,which enables isotropic resolution improvement.展开更多
Super-resolution structured illumination microscopy(SR-SIM)relies heavily on post-processing reconstruction to obtain high-quality SR images from raw data.Although many SIM reconstruction algorithms have been develope...Super-resolution structured illumination microscopy(SR-SIM)relies heavily on post-processing reconstruction to obtain high-quality SR images from raw data.Although many SIM reconstruction algorithms have been developed to recover fine cellular structures with high fidelity even from the noisy data,whether the pixel intensities of reconstructed SR images are still proportional to the original fluorescence intensity has been less explored.The linearity between the intensity before and after reconstruction is de fined as the intensity fidelity.Here,we proposed a method to evaluate the reconstructed SR image intensity fidelity at different spatial frequencies.With the proposed metric,we systematically investigated the impact of the key factors on the intensity fidelity in the standard Wiener-SIM reconstructions with simulated data,then evaluated the intensity fidelity of the SR images reconstructed by representative open-source packages.Our work provides a reference for SR-SIM image intensity fidelity improvement.展开更多
Autophagy is a conserved intracellular degradation system that plays a dual role in cell death;thus,therapies targeting autophagy in cancer are somewhat controversial.Ferroptosis is a new form of regulated cell death ...Autophagy is a conserved intracellular degradation system that plays a dual role in cell death;thus,therapies targeting autophagy in cancer are somewhat controversial.Ferroptosis is a new form of regulated cell death featured with the iron-dependent accumulation of lethal lipid ROS.This pathway is morphologically,biochemically and genetically distinct from other forms of cell death.Accumulating studies have revealed crosstalk between autophagy and ferroptosis at the molecular level.In this review,we summarize the mechanisms of ferroptosis and autophagy,and more importantly,their roles in the drug resistance of cancer.Numerous connections between ferroptosis and autophagy have been revealed,and a strong causal relationship exists wherein one process controls the other and can be utilized as potential therapeutic targets for cancer.The elucidation of when and how to modulate their crosstalk using therapeutic strategies depends on an understanding of the fine-tuned switch between ferroptosis and autophagy,and approaches designed to manipulate the intensity of autophagy might be the key.展开更多
Structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling methods.Structured ...Structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling methods.Structured illumination can be obtained by either laser interference or projection of fringe patterns.Here,we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device(DMD)which can be directly attached to most commercial invertedffuorescent microscopes and update it into a SIM system.The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured lightfield were studied.With the optimized fringe pattern,1:6×resolution improvement could be obtained with high-end oil objectives.Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated.Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in thefield of life science and medicine.展开更多
The influences of steam and sulfide on the efficiency of NOx reduction using ammonia(NH_(3))over the nanometer-class V-W/Ti catalyst in conditions of high temperature is experimentally investigated using a steady-flow...The influences of steam and sulfide on the efficiency of NOx reduction using ammonia(NH_(3))over the nanometer-class V-W/Ti catalyst in conditions of high temperature is experimentally investigated using a steady-flow reactor.The results showed that selective catalytic reduction(SCR)is inhibited by H_(2)O at low temperature,but higher NO conversion efficiency is achieved at high temperature since the reaction of NH_(3) oxidized by O_(2) to NOx is inhibited by H_(2)O.The activity of SCR is promoted by SO_(2) in the temperature range of 200~500℃,the NO conversion efficiency was improved to 98%from 94%by adding SO_(2).SCR would be improved at 350~500℃ when H_(2)O and SO_(2) exist at the same time.Furthermore,the positive influence to the NOx conversion was proved in the presence of H_(2)O and SO_(2) as a result of the European Stationary Cycle test.展开更多
The zebrafish embryos were widely employed in genetics,development and drug discovery studies as miniatured animal models.Sorting of two-color fluorescent embryos is often required in large-scale experiments but it is...The zebrafish embryos were widely employed in genetics,development and drug discovery studies as miniatured animal models.Sorting of two-color fluorescent embryos is often required in large-scale experiments but it is challenging to manually sort with high efficiency.Here,we reported a high-throughput sorting system for two-color fluorescent zebraflsh embryos.The embryos can be automatically loaded from a sample pool and sorted based on the average fluorescent intensity.The two-color fluorescent signals were split into two lines and detected by an area array camera.The system achieves the sorting of 100 embryos in less than 10 min with an accuracy of greater than 95%.展开更多
RNA-binding proteins(RBPs)act as crucial regulators of gene expression within cells,exerting precise control over processes such as RNA splicing,transport,localization,sta-bility,and translation through their specific...RNA-binding proteins(RBPs)act as crucial regulators of gene expression within cells,exerting precise control over processes such as RNA splicing,transport,localization,sta-bility,and translation through their specific binding to RNA molecules.The diversity and complexity of RBPs are particularly significant in cancer biology,as they directly impact a multitude of RNA metabolic events closely associated with tumor initiation and progression.The fragile X mental retardation protein(FMRP),as a member of the RBP family,is central to the neurodevelopmental disorder fragile X syndrome and increasingly recognized in the modulation of cancer biology through its influence on RNA metabolism.The protein’s versa-tility,stemming from its diverse RNA-binding domains,enables it to govern a wide array of transcript processing events.Modifications in FMRP’s expression or localization have been asso-ciated with the regulation of mRNAs linked to various processes pertinent to cancer,including tumor proliferation,metastasis,epithelialemesenchymal transition,cellular senescence,chemotherapy/radiotherapy resistance,and immunotherapy evasion.In this review,we emphasize recent findings and analyses that suggest contrasting functions of this protein fam-ily in tumorigenesis.Our knowledge of the proteins that are regulated by FMRP is rapidly growing,and this has led to the identification of multiple targets for therapeutic intervention of cancer,some of which have already moved into clinical trials or clinical practice.展开更多
The tumor microenvironment is proposed to contribute substantially to the progression of cancers,including breast cancer.Cancer-associated fibroblasts(CAFs)are the most abundant components of the tumor microenvironmen...The tumor microenvironment is proposed to contribute substantially to the progression of cancers,including breast cancer.Cancer-associated fibroblasts(CAFs)are the most abundant components of the tumor microenvironment.Studies have revealed that CAFs in breast cancer originate from several types of cells and promote breast cancer malignancy by secreting factors,generating exosomes,releasing nutrients,reshaping the extracellular matrix,and suppressing the function of immune cells.CAFs are also becoming therapeutic targets for breast cancer due to their specific distribution in tumors and their unique biomarkers.Agents interrupting the effect of CAFs on surrounding cells have been developed and applied in clinical trials.Here,we reviewed studies examining the heterogeneity of CAFs in breast cancer and expression patterns of CAF markers in different subtypes of breast cancer.We hope that summarizing CAFrelated studies from a historical perspective will help to accelerate the development of CAF-targeted therapeutic strategies for breast cancer.展开更多
Super-resolution structured illumination microscopy(SR-SIM)has become a widely used nanoscopy technique for rapid,long-term,and multi-color imaging of live cells.Precise but troublesome determination of the illuminati...Super-resolution structured illumination microscopy(SR-SIM)has become a widely used nanoscopy technique for rapid,long-term,and multi-color imaging of live cells.Precise but troublesome determination of the illumination pattern parameters is a prerequisite for Wiener-deconvolution-based SR-SIM image reconstruction.Here,we present a direct reconstruction SIM algorithm(direct-SIM)with an initial spatial-domain reconstruction followed by frequency-domain spectrum optimization.Without any prior knowledge of illumination patterns and bypassing the artifact-sensitive Wiener deconvolution procedures,resolution-doubled SR images could be reconstructed by direct-SIM free of common artifacts,even for the raw images with large pattern variance in the field of view(FOV).Direct-SIM can be applied to previously difficult scenarios such as very sparse samples,periodic samples,very small FOV imaging,and stitched large FOV imaging.展开更多
It was showed that the sedimentary environment of lacustrine basins is more susceptible to basin size than that of marine basins,leading to frequent changes in sedimentary environment and large differences in sediment...It was showed that the sedimentary environment of lacustrine basins is more susceptible to basin size than that of marine basins,leading to frequent changes in sedimentary environment and large differences in sediment supply and types.Based on the comprehensive analysis of clay minerals,spore pollen,major elements and trace elements,the sedimentary environment of Dongying Formation and its evolution in Bozhong Sag were studied.The results showed that during the sedimentary period of Dongying Formation,the study area was in a subtropical warm and humid climate as a whole.In addition,the vertical distribution of spore pollen and algae was divided into four zones and each one represents different sedimentary environments.Combination zone I is characterized by a large number of spore pollen and algae,including juglanspollenites verus,ulmipollemites minor,abietineaepollenites and pinuspollenites,taxodiaceaepollenites hiatus,leiosphaeridia and granodiscus,and it indicates a relatively hot and dry climatic;Combination zone II mainly contains juglanspollenites verus,ulmipollemites minor,abietineaepollenites and pinuspollenites,pediastrum and leiosphaeridia.Compared with I,the number of spore pollen and algae decreases,but the number of pediastrum increases a lot,which represents warm and humid climate.The combination zone III is dominated by abietineaepollenites and pinuspollenites and chaetosphaeridium,and the species and quantity decrease significantly.It indicates a relatively hot and humid climate at that time.Combination zone IV contains a small amount of spore pollen and algae,and it means the climate was warm and humid at that period.The frequent changes of sedimentary environment in lacustrine basins are influenceded by regional tectonic environment and basin scale.Using S/A and E-T parameters,the paleoclimate in the study area was delineated and 10 sedimentary environment interfaces were identified,which further illustrated the characteristics of frequent fluctuations of sedimentary environment in lacustrine basins.展开更多
The Kuqa Depression in Tarim Basin develops fan deltaic,braided river deltaic,and lacustrine sedi-mentation,espeially extensive braided river deltaic sedimentation,in the Cretaceous-Paleogene Sys-tems.However,fine sed...The Kuqa Depression in Tarim Basin develops fan deltaic,braided river deltaic,and lacustrine sedi-mentation,espeially extensive braided river deltaic sedimentation,in the Cretaceous-Paleogene Sys-tems.However,fine sedimentary pattern of the Kuqa Depression and North Tarim Uplift belt since the Middle Cenozoic still needs to be studied.As a combination zone of the Kuqa Depression and North Tarim Uplift,the sedimentary characteristics of the DB area in the southern margin of the east of the Kuqa depression have been paid more attention.To better understand the sedimentary framework in the Kuqa Depression and North Tarim Uplift in the Mesozoic and Cenozoic,through joint geologic-geophysical study,sedime ntation of sandstone at the bottom of the Paleogene Suweiyi Formation in the DB area and its peripheral area is investigated.Sedimentary facies and sandstone distribution in the area are identified through core observation,component analysis,logging interpretation and seismic inversion.Based on seismic facies analysis,sedimentary facies distribution in the area is delineated.Results show that sandstone at the bottom of the Paleogene Suweiyi Fommation in the DB area and its peripheral area is developed in shore shallow lacustrine beach-bar facies.The beach-bar arenaceous sediments are mainly distnibuted in the southern DB area and the shallow lacustrine mudstone is developed in northern DB area,showing obvious north south di ferentiation charactenistics.展开更多
Background Parkinson's disease(PD)patients suffer from progressive gray matter volume(GMV)loss,but whether distinct patterns of atrophy progression exist within PD are still unclear.Objective This study aims to id...Background Parkinson's disease(PD)patients suffer from progressive gray matter volume(GMV)loss,but whether distinct patterns of atrophy progression exist within PD are still unclear.Objective This study aims to identify PD subtypes with different rates of GMV loss and assess their association with clinical progression.Methods This study included 107 PD patients(mean age:60.06±9.98 years,70.09%male)with baseline and≥3-year follow-up structural MRI scans.A linear mixed-effects model was employed to assess the rates of regional GMV loss.Hierarchical cluster analysis was conducted to explore potential subtypes based on individual rates of GMV loss.Clinical score changes were then compared across these subtypes.Results Two PD subtypes were identified based on brain atrophy rates.Subtype 1(n=63)showed moderate atrophy,notably in the prefrontal and lateral temporal lobes,while Subtype 2(n=44)had faster atrophy across the brain,particularly in the lateral temporal region.Furthermore,subtype 2 exhibited faster deterioration in non-motor(MDS-UPDRS-PartⅠ,β=1.26±0.18,P=0.016)and motor(MDS-UPDRS-PartⅡ,β=1.34±0.20,P=0.017)symptoms,autonomic dysfunction(SCOPA-AUT,β=1.15±0.22,P=0.043),memory(HVLT-Retention,β=−0.02±0.01,P=0.016)and depression(GDS,β=0.26±0.083,P=0.019)compared to subtype 1.Conclusion The study has identified two PD subtypes with distinct patterns of atrophy progression and clinical progression,which may have implications for developing personalized treatment strategies.展开更多
基金supported by the National Natural Science Foundation of China[Grant Nos.62205367 and 62141506]the Suzhou Basic Research Pilot Project[Grant Nos.SSD2023006 and SJC2021013]the National Key Research and Development Program of China[Grant No.2023YFF1205700].
文摘Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope(SR-SIM).However,the imperfect optical components can depolarize the laser beams hence degenerating the modulation depth.Here,we first presented a direct measurement method designed to estimate the modulation depth more precisely by shifting illumination patterns with equal phase steps.This measurement method greatly reduces the dependence of modulation depths on the samples,and then developed a polarization optimization method to achieve high modulation depth at all orientations by actively and quantitatively compensating for the additional phase difference using a combination of waveplate and a liquid crystal variable retarder(LCVR).Experimental results demonstrate that our method can achieve illumination patterns with modulation depth higher than 0.94 at three orientations with only one LCVR voltage,which enables isotropic resolution improvement.
基金supported by the National Natural Science Foundation of China[Grant Nos.62205367 and 62141506]Suzhou Basic Research Pilot Project[Grant Nos.SSD2023006 and SJC2021013]Jiangsu Provincial Key Research and Development Program[Grant No.BE2020664].
文摘Super-resolution structured illumination microscopy(SR-SIM)relies heavily on post-processing reconstruction to obtain high-quality SR images from raw data.Although many SIM reconstruction algorithms have been developed to recover fine cellular structures with high fidelity even from the noisy data,whether the pixel intensities of reconstructed SR images are still proportional to the original fluorescence intensity has been less explored.The linearity between the intensity before and after reconstruction is de fined as the intensity fidelity.Here,we proposed a method to evaluate the reconstructed SR image intensity fidelity at different spatial frequencies.With the proposed metric,we systematically investigated the impact of the key factors on the intensity fidelity in the standard Wiener-SIM reconstructions with simulated data,then evaluated the intensity fidelity of the SR images reconstructed by representative open-source packages.Our work provides a reference for SR-SIM image intensity fidelity improvement.
基金supported by the National Natural Science Foundation of China (Grant No. 81602471, 81672729, 81672729, 81874380 and 81672932)Zhejiang Provincial Natural Science Foundation of China under Grants (Grant No. LY19H160055, LY19H160059)+5 种基金by Zheng Shu Medical Elite Scholarship Fundby grant from sub-project of China National Program on Key Basic Research Project (973 Program) (Grant No. 2014CB744505)Zhejiang Provincial Natural Science Foundation of China for Distinguished Young Scholars (Grant No. LR18H160001)Zhejiang Province Medical Science and Technology Project (Grant No. 2017RC007)Talent Project of Zhejiang Association for Science and Technology (Grant No. 2017YCGC002)Zhejiang Province Science and Technology Project of TCM (Grant No. 2019ZZ016)
文摘Autophagy is a conserved intracellular degradation system that plays a dual role in cell death;thus,therapies targeting autophagy in cancer are somewhat controversial.Ferroptosis is a new form of regulated cell death featured with the iron-dependent accumulation of lethal lipid ROS.This pathway is morphologically,biochemically and genetically distinct from other forms of cell death.Accumulating studies have revealed crosstalk between autophagy and ferroptosis at the molecular level.In this review,we summarize the mechanisms of ferroptosis and autophagy,and more importantly,their roles in the drug resistance of cancer.Numerous connections between ferroptosis and autophagy have been revealed,and a strong causal relationship exists wherein one process controls the other and can be utilized as potential therapeutic targets for cancer.The elucidation of when and how to modulate their crosstalk using therapeutic strategies depends on an understanding of the fine-tuned switch between ferroptosis and autophagy,and approaches designed to manipulate the intensity of autophagy might be the key.
基金The study was funded by the National Key Technologies R&D Program of China(2018YFC0114800 and 2017YFC0109900)the Natural Science Foundation of China(NSFC)(61405238)+1 种基金the Natural Science Foundation of Jiangsu Province(BK20141206)the Key Technologies R&D Program of Jiangsu Province(BE2018666).
文摘Structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling methods.Structured illumination can be obtained by either laser interference or projection of fringe patterns.Here,we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device(DMD)which can be directly attached to most commercial invertedffuorescent microscopes and update it into a SIM system.The effects of the period and duty cycle of fringe patterns on the modulation depth of the structured lightfield were studied.With the optimized fringe pattern,1:6×resolution improvement could be obtained with high-end oil objectives.Multicolor imaging and dynamics of subcellular organelles in live cells were also demonstrated.Our method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in thefield of life science and medicine.
基金supported by the Ministry of Science and Technology of the People’s Republic of China[Grant number 2017YFC0211305]the Science Fund of State Key Laboratory of Engine Reliability[Grant number Skler-201610].
文摘The influences of steam and sulfide on the efficiency of NOx reduction using ammonia(NH_(3))over the nanometer-class V-W/Ti catalyst in conditions of high temperature is experimentally investigated using a steady-flow reactor.The results showed that selective catalytic reduction(SCR)is inhibited by H_(2)O at low temperature,but higher NO conversion efficiency is achieved at high temperature since the reaction of NH_(3) oxidized by O_(2) to NOx is inhibited by H_(2)O.The activity of SCR is promoted by SO_(2) in the temperature range of 200~500℃,the NO conversion efficiency was improved to 98%from 94%by adding SO_(2).SCR would be improved at 350~500℃ when H_(2)O and SO_(2) exist at the same time.Furthermore,the positive influence to the NOx conversion was proved in the presence of H_(2)O and SO_(2) as a result of the European Stationary Cycle test.
基金the National Natural Science Foundation of China(No.62205368)the Suzhou Basic Research Pilot Project(SJC2021013)the Key Research and Development Program of Jiangsu Province(BE2020664).
文摘The zebrafish embryos were widely employed in genetics,development and drug discovery studies as miniatured animal models.Sorting of two-color fluorescent embryos is often required in large-scale experiments but it is challenging to manually sort with high efficiency.Here,we reported a high-throughput sorting system for two-color fluorescent zebraflsh embryos.The embryos can be automatically loaded from a sample pool and sorted based on the average fluorescent intensity.The two-color fluorescent signals were split into two lines and detected by an area array camera.The system achieves the sorting of 100 embryos in less than 10 min with an accuracy of greater than 95%.
基金supported by the National Natural Science Foundation of China(No.82000212)the Natural Science Foundation of Zhejiang Province,China(No.LQ21H160022)+1 种基金the Medical Health Science and Technology Project of Zhejiang Provincial Health Commission(China)(No.2024KY989)Zhejiang Provincial Science and Technology Program for Traditional Chinese Medicine(No.2025ZR142).
文摘RNA-binding proteins(RBPs)act as crucial regulators of gene expression within cells,exerting precise control over processes such as RNA splicing,transport,localization,sta-bility,and translation through their specific binding to RNA molecules.The diversity and complexity of RBPs are particularly significant in cancer biology,as they directly impact a multitude of RNA metabolic events closely associated with tumor initiation and progression.The fragile X mental retardation protein(FMRP),as a member of the RBP family,is central to the neurodevelopmental disorder fragile X syndrome and increasingly recognized in the modulation of cancer biology through its influence on RNA metabolism.The protein’s versa-tility,stemming from its diverse RNA-binding domains,enables it to govern a wide array of transcript processing events.Modifications in FMRP’s expression or localization have been asso-ciated with the regulation of mRNAs linked to various processes pertinent to cancer,including tumor proliferation,metastasis,epithelialemesenchymal transition,cellular senescence,chemotherapy/radiotherapy resistance,and immunotherapy evasion.In this review,we emphasize recent findings and analyses that suggest contrasting functions of this protein fam-ily in tumorigenesis.Our knowledge of the proteins that are regulated by FMRP is rapidly growing,and this has led to the identification of multiple targets for therapeutic intervention of cancer,some of which have already moved into clinical trials or clinical practice.
基金National Natural Science Foundation of China,Grant/Award Numbers:81602471,81672729,81972453,81972597Natural Science Foundation of Zhejiang Province,Grant/Award Numbers:LY19H160055,LY19H160059,LR22H160011+2 种基金Natural Science Foundation of Ningbo,Grant/Award Number:2019A610315Cixi Agricultural and Social Development Science and Technology Project,Grant/Award Number:CN2020006Zheng Shu Medical Elite Scholarship Fund。
文摘The tumor microenvironment is proposed to contribute substantially to the progression of cancers,including breast cancer.Cancer-associated fibroblasts(CAFs)are the most abundant components of the tumor microenvironment.Studies have revealed that CAFs in breast cancer originate from several types of cells and promote breast cancer malignancy by secreting factors,generating exosomes,releasing nutrients,reshaping the extracellular matrix,and suppressing the function of immune cells.CAFs are also becoming therapeutic targets for breast cancer due to their specific distribution in tumors and their unique biomarkers.Agents interrupting the effect of CAFs on surrounding cells have been developed and applied in clinical trials.Here,we reviewed studies examining the heterogeneity of CAFs in breast cancer and expression patterns of CAF markers in different subtypes of breast cancer.We hope that summarizing CAFrelated studies from a historical perspective will help to accelerate the development of CAF-targeted therapeutic strategies for breast cancer.
基金National Natural Science Foundation of China[grant no.62141506,62205367]National Key Research and Development Program of China[grant no.2017YFC0110100]+1 种基金Suzhou Basic Research Pilot Project[grant no.SJC2021013]Jiangsu Provincial Key Research and Development Program[grant no.BE2020664].
文摘Super-resolution structured illumination microscopy(SR-SIM)has become a widely used nanoscopy technique for rapid,long-term,and multi-color imaging of live cells.Precise but troublesome determination of the illumination pattern parameters is a prerequisite for Wiener-deconvolution-based SR-SIM image reconstruction.Here,we present a direct reconstruction SIM algorithm(direct-SIM)with an initial spatial-domain reconstruction followed by frequency-domain spectrum optimization.Without any prior knowledge of illumination patterns and bypassing the artifact-sensitive Wiener deconvolution procedures,resolution-doubled SR images could be reconstructed by direct-SIM free of common artifacts,even for the raw images with large pattern variance in the field of view(FOV).Direct-SIM can be applied to previously difficult scenarios such as very sparse samples,periodic samples,very small FOV imaging,and stitched large FOV imaging.
基金This work is supported by the the National Natural Science Foundation of China(No.51804038,51704033)the PetroChina Innovation Foundation(Grant No.2018D-5007-0210).
文摘It was showed that the sedimentary environment of lacustrine basins is more susceptible to basin size than that of marine basins,leading to frequent changes in sedimentary environment and large differences in sediment supply and types.Based on the comprehensive analysis of clay minerals,spore pollen,major elements and trace elements,the sedimentary environment of Dongying Formation and its evolution in Bozhong Sag were studied.The results showed that during the sedimentary period of Dongying Formation,the study area was in a subtropical warm and humid climate as a whole.In addition,the vertical distribution of spore pollen and algae was divided into four zones and each one represents different sedimentary environments.Combination zone I is characterized by a large number of spore pollen and algae,including juglanspollenites verus,ulmipollemites minor,abietineaepollenites and pinuspollenites,taxodiaceaepollenites hiatus,leiosphaeridia and granodiscus,and it indicates a relatively hot and dry climatic;Combination zone II mainly contains juglanspollenites verus,ulmipollemites minor,abietineaepollenites and pinuspollenites,pediastrum and leiosphaeridia.Compared with I,the number of spore pollen and algae decreases,but the number of pediastrum increases a lot,which represents warm and humid climate.The combination zone III is dominated by abietineaepollenites and pinuspollenites and chaetosphaeridium,and the species and quantity decrease significantly.It indicates a relatively hot and humid climate at that time.Combination zone IV contains a small amount of spore pollen and algae,and it means the climate was warm and humid at that period.The frequent changes of sedimentary environment in lacustrine basins are influenceded by regional tectonic environment and basin scale.Using S/A and E-T parameters,the paleoclimate in the study area was delineated and 10 sedimentary environment interfaces were identified,which further illustrated the characteristics of frequent fluctuations of sedimentary environment in lacustrine basins.
文摘The Kuqa Depression in Tarim Basin develops fan deltaic,braided river deltaic,and lacustrine sedi-mentation,espeially extensive braided river deltaic sedimentation,in the Cretaceous-Paleogene Sys-tems.However,fine sedimentary pattern of the Kuqa Depression and North Tarim Uplift belt since the Middle Cenozoic still needs to be studied.As a combination zone of the Kuqa Depression and North Tarim Uplift,the sedimentary characteristics of the DB area in the southern margin of the east of the Kuqa depression have been paid more attention.To better understand the sedimentary framework in the Kuqa Depression and North Tarim Uplift in the Mesozoic and Cenozoic,through joint geologic-geophysical study,sedime ntation of sandstone at the bottom of the Paleogene Suweiyi Formation in the DB area and its peripheral area is investigated.Sedimentary facies and sandstone distribution in the area are identified through core observation,component analysis,logging interpretation and seismic inversion.Based on seismic facies analysis,sedimentary facies distribution in the area is delineated.Results show that sandstone at the bottom of the Paleogene Suweiyi Fommation in the DB area and its peripheral area is developed in shore shallow lacustrine beach-bar facies.The beach-bar arenaceous sediments are mainly distnibuted in the southern DB area and the shallow lacustrine mudstone is developed in northern DB area,showing obvious north south di ferentiation charactenistics.
基金funded by grants from the National Natural Science Foundation of China(82071997)Shanghai Rising-Star Program(21QA1408700),111 Project(B18015)the Natural Science Foundation of Shanghai(23ZR1406000).
文摘Background Parkinson's disease(PD)patients suffer from progressive gray matter volume(GMV)loss,but whether distinct patterns of atrophy progression exist within PD are still unclear.Objective This study aims to identify PD subtypes with different rates of GMV loss and assess their association with clinical progression.Methods This study included 107 PD patients(mean age:60.06±9.98 years,70.09%male)with baseline and≥3-year follow-up structural MRI scans.A linear mixed-effects model was employed to assess the rates of regional GMV loss.Hierarchical cluster analysis was conducted to explore potential subtypes based on individual rates of GMV loss.Clinical score changes were then compared across these subtypes.Results Two PD subtypes were identified based on brain atrophy rates.Subtype 1(n=63)showed moderate atrophy,notably in the prefrontal and lateral temporal lobes,while Subtype 2(n=44)had faster atrophy across the brain,particularly in the lateral temporal region.Furthermore,subtype 2 exhibited faster deterioration in non-motor(MDS-UPDRS-PartⅠ,β=1.26±0.18,P=0.016)and motor(MDS-UPDRS-PartⅡ,β=1.34±0.20,P=0.017)symptoms,autonomic dysfunction(SCOPA-AUT,β=1.15±0.22,P=0.043),memory(HVLT-Retention,β=−0.02±0.01,P=0.016)and depression(GDS,β=0.26±0.083,P=0.019)compared to subtype 1.Conclusion The study has identified two PD subtypes with distinct patterns of atrophy progression and clinical progression,which may have implications for developing personalized treatment strategies.
