The present study aimed to evaluate the clinical outcomes of magnetic-activated cell sorting(MACS)in sperm preparation for male subjects with a sperm DNA fragmentation index(DFI)≥30%.A total of 86 patients who had un...The present study aimed to evaluate the clinical outcomes of magnetic-activated cell sorting(MACS)in sperm preparation for male subjects with a sperm DNA fragmentation index(DFI)≥30%.A total of 86 patients who had undergone their first long-term long protocol were selected.The protocol involved in vitro fertilization(IVF)and intracytoplasmic sperm injection(ICSI)cycles,and the patients were divided into the MACS or control groups.The MACS group included sperm samples analyzed with MACS that were combined with density gradient centrifugation(DGC)and the swim-up(SU)technique(n=39),and the control group included sperm samples prepared using standard techniques(DGC and SU;n=41).No differences were noted with regard to basic clinical characteristics,number of oocytes retrieved,normal fertilization rate,cleavage rate,or transplantable embryo rate between the two groups in IVF/ICSI.In addition,the clinical pregnancy and implantation rates of the first embryo transfer cycles indicated no significant differences between the two groups.However,there was a tendency to improve the live birth rate(LBR)of the first embryo transfer cycle(63.2%vs 53.9%)and the cumulative LBR(79.5%vs 70.7%)in the MACS group compared with the control group.Moreover,the number of transferred embryos(mean±standard deviation[s.d.]:1.7±0.7 vs 2.3±1.6)and the transfer number of each retrieved cycle(mean±s.d.:1.2±0.5 vs 1.6±0.8)were significantly lower in the MACS group than those in the control group.Thus,the selection of nonapoptotic spermatozoa by MACS for higher sperm DFI could improve assisted reproductive clinical outcomes.展开更多
Objective To analyze the fertilization rate, embryo development and clinical outcome of oocytes with abnormal zona pellucida after in vitro fertilization （IVF） or intracytoplasmic sperm injection （1CS1）. Methods ...Objective To analyze the fertilization rate, embryo development and clinical outcome of oocytes with abnormal zona pellucida after in vitro fertilization （IVF） or intracytoplasmic sperm injection （1CS1）. Methods A retrospective analysis included a total of 43 cycles （27 IVF cycles and 16 ICSI cycles） in which oocytes displaying abnormal zona pellucida were retrieved between January 2006 and December 2011. The fertilization rate, embryo quality, and the cumulative clinical pregnancy rate were analyzed. Results Rescue ICSI was applied in 2 7 IVF cycles in which failed extrusion of the second polar body after conventional IVF was observed, and of them, complete failure to fertilize occurred in 23 IVF cycles. The fertilization rate and the normal fertilization rate for IVF （64.83% and 59.32%, respectively） were significantly lower than those for ICS1 （85.19% and 79.01%, respectively）, whereas the cleavage rate （94.12%） with IVF did not differ significantly from that with ICSI （95.65%, P〉0. 05）. The percentages of good-quality embryos in 1VF group （52. 67%） and 1CSl group （43.75%） also did not differ significantly （P〉0.05）. Although the rates of implantation and pregnancy appeared to be greater in IVF group （33.33% and 40.00%, respectively） compared with those in ICSI group （25.00% and 35. 71%, respectively）, the differences were not significant （P〉0.05）.Conclusions ICSI should be carried out for oocytes with abnormal zona pellucida, for which the risk of lVF failure is high. Rescue ICSI improves the likelihood of fertilization of oocytes with abnormal zona pellucida, but cannot improve the clinical outcome.展开更多
基金supported by the National Natural Science Foundation of China(No.81801518 and No.82071646).
文摘The present study aimed to evaluate the clinical outcomes of magnetic-activated cell sorting(MACS)in sperm preparation for male subjects with a sperm DNA fragmentation index(DFI)≥30%.A total of 86 patients who had undergone their first long-term long protocol were selected.The protocol involved in vitro fertilization(IVF)and intracytoplasmic sperm injection(ICSI)cycles,and the patients were divided into the MACS or control groups.The MACS group included sperm samples analyzed with MACS that were combined with density gradient centrifugation(DGC)and the swim-up(SU)technique(n=39),and the control group included sperm samples prepared using standard techniques(DGC and SU;n=41).No differences were noted with regard to basic clinical characteristics,number of oocytes retrieved,normal fertilization rate,cleavage rate,or transplantable embryo rate between the two groups in IVF/ICSI.In addition,the clinical pregnancy and implantation rates of the first embryo transfer cycles indicated no significant differences between the two groups.However,there was a tendency to improve the live birth rate(LBR)of the first embryo transfer cycle(63.2%vs 53.9%)and the cumulative LBR(79.5%vs 70.7%)in the MACS group compared with the control group.Moreover,the number of transferred embryos(mean±standard deviation[s.d.]:1.7±0.7 vs 2.3±1.6)and the transfer number of each retrieved cycle(mean±s.d.:1.2±0.5 vs 1.6±0.8)were significantly lower in the MACS group than those in the control group.Thus,the selection of nonapoptotic spermatozoa by MACS for higher sperm DFI could improve assisted reproductive clinical outcomes.
基金supported by Jiangsu Province’s Outstanding Medical Academic Leader program,Jiangsu Health Department,No.LJ201102
文摘Objective To analyze the fertilization rate, embryo development and clinical outcome of oocytes with abnormal zona pellucida after in vitro fertilization （IVF） or intracytoplasmic sperm injection （1CS1）. Methods A retrospective analysis included a total of 43 cycles （27 IVF cycles and 16 ICSI cycles） in which oocytes displaying abnormal zona pellucida were retrieved between January 2006 and December 2011. The fertilization rate, embryo quality, and the cumulative clinical pregnancy rate were analyzed. Results Rescue ICSI was applied in 2 7 IVF cycles in which failed extrusion of the second polar body after conventional IVF was observed, and of them, complete failure to fertilize occurred in 23 IVF cycles. The fertilization rate and the normal fertilization rate for IVF （64.83% and 59.32%, respectively） were significantly lower than those for ICS1 （85.19% and 79.01%, respectively）, whereas the cleavage rate （94.12%） with IVF did not differ significantly from that with ICSI （95.65%, P〉0. 05）. The percentages of good-quality embryos in 1VF group （52. 67%） and 1CSl group （43.75%） also did not differ significantly （P〉0.05）. Although the rates of implantation and pregnancy appeared to be greater in IVF group （33.33% and 40.00%, respectively） compared with those in ICSI group （25.00% and 35. 71%, respectively）, the differences were not significant （P〉0.05）.Conclusions ICSI should be carried out for oocytes with abnormal zona pellucida, for which the risk of lVF failure is high. Rescue ICSI improves the likelihood of fertilization of oocytes with abnormal zona pellucida, but cannot improve the clinical outcome.
