As a type of new carbon-based nanomaterials,carbon dots(CDs)possess exceptional optical properties,making them highly desirable for use in fluorescent sensors.However,the CDs with deep-red(DR)or near-infrared(NIR)emis...As a type of new carbon-based nanomaterials,carbon dots(CDs)possess exceptional optical properties,making them highly desirable for use in fluorescent sensors.However,the CDs with deep-red(DR)or near-infrared(NIR)emission have rarely been reported.In this work,we prepared deep-red emissive fluorine-doped carbon quantum dots(F-CDs)by introducing a precursor simultaneously containing fluorine and amidogen.The synergistic effect of nitrogen doping and D-π-A pattern production contributed to the maximum emission of F-CDs at 636 nm with an absolute quantum yield of 36.00%±0.68%.Moreover,we designed an F-CDs-based fluorescence assay to determine the content of hypochlorite(ClO^(-)),with a limit of detection(LOD)as low as 15.4 nmol/L,indicating the high sensitivity of F-CDs to ClO^(-).In real samples,the F-CDs-based fluorescent sensor exhibited excellent sensitivity and selectivity in the detection of ClO^(-),with an error below 2%,suggesting their great potential in daily life.In cancer cell imaging,the F-CDs not only demonstrated high sensitivity to ClO^(-)but also exhibited excellent mitochondria targeting,as evidenced by the high Pearson's correlation coefficient(PCC)of 0.93 in colocalization analysis.The work presented here suggests the great potential of replacing commercial dyes with F-CDs for highly specific mitochondria labeling and cell imaging.展开更多
Dental,oral,and craniofacial diseases can substantially impact the quality of human life,thereby posing a serious public health concern.Although conventional therapies such as surgery have solved these problems largel...Dental,oral,and craniofacial diseases can substantially impact the quality of human life,thereby posing a serious public health concern.Although conventional therapies such as surgery have solved these problems largely,the prognosis of patients is not always satisfactory.Cell membrane-coated nanoparticles(CMCNPs)carry nanodrugs with the help of natural cell membranes,therefore utilizing their remarkable ability to interface and interact with their surrounding environment.These nanoparticles have demonstrated substantial advantages in drug targeting,prolonging blood circulation time,penetrating biofilms,and immune escape.With the assistance of CMCNPs,the therapeutic effects of dental,oral,and craniofacial diseases can reach a higher level.CMCNPs have been applied for dental,oral,and craniofacial diseases for various conditions such as head and neck cancer,periodontal disease,and oral biosignal detection.For the therapies of head and neck cancer,CMCNPs have been widely utilized as a tool of chemotherapy,phototherapy,and immunotherapy,while yet to be exploited in imaging technique.In the end,we summarized the challenges and prospectives of CMCNPs for dental,oral,and craniofacial diseases:large-scale production with uniform standards and high quantity,extensive application directions in dental,oral,and craniofacial regions(implant,endodontics),and the promotion of its clinical application.展开更多
In a recent study published in Science,Tsvetkov et al1.shed a light on a new form of cell death,copper-dependent cell death(termed cuproptosis).They defined“cuproptosis”as a nonapoptotic cell death pathway.They have...In a recent study published in Science,Tsvetkov et al1.shed a light on a new form of cell death,copper-dependent cell death(termed cuproptosis).They defined“cuproptosis”as a nonapoptotic cell death pathway.They have demonstrated that copper directly binds to lipoylated components of the tricarboxylic acid(TCA)cycle.Then aggregation of these copper-bound,lipoylated mitochondrial proteins and subsequent Fe-S cluster protein loss triggered proteotoxic stress and a distinct form of cell death.展开更多
Dental,oral,and craniofacial diseases jeopardize health and reduce the quality of life.Accessing disease-related signals in advance is beneficial to prevent the occurrence or progression of those diseases.However,the ...Dental,oral,and craniofacial diseases jeopardize health and reduce the quality of life.Accessing disease-related signals in advance is beneficial to prevent the occurrence or progression of those diseases.However,the inconvenience of periodical in-hospital examinations and the difficulty of sustaining daily health monitoring challenge personal compliance and possibly lead to limited prevention or treatment.Medical flexible electronics are electric devices fabricated on soft and extensible substrates to fit the human skin and enable non-invasive continuous monitoring of biophysical/biochemical signals.They provide the possibility of long-term,continuous,comfortable,and wireless healthcare monitoring and are expected to alleviate time and economic consumption by avoiding in-hospital examinations and treatment.Therefore,flexible electronics have emerged for early diagnosis and disease monitoring in stomatology.It is noteworthy that special biophysical/biochemical characteristics and the environment of dental,oral,and craniofacial areas bring distinct challenges that flexible electronics need to address ingeniously to ensure their stability,selectivity,and sensitivity.This review summaries flexible electronics and their specificity when used in dental,oral,and craniofacial applications,including monitoring saliva or cavity-gas related biosignals,sensing the mechanical fluctuation from facial muscle/respiratory activities or orthodontic forces,and executing special functions in the prevention or postoperative recovery of relevant diseases.Furthermore,after analyzing current challenges and proposing potential solutions,the“5I”principles of imperceptibility,intelligence,individualization,integration,and inexpensiveness are presented to help guide the future development of flexible electronics and promote their commercialization for dental,oral,and craniofacial medicine.展开更多
The recurrence of head and neck squamous cell carcinoma(HNSCC)after surgical resection continues to pose a major challenge to cancer treatment.Advanced HNSCC exhibits a low response rate to immune checkpoint blockade(...The recurrence of head and neck squamous cell carcinoma(HNSCC)after surgical resection continues to pose a major challenge to cancer treatment.Advanced HNSCC exhibits a low response rate to immune checkpoint blockade(ICB),while photothermal therapy(PTT)can increase the infiltration of immune cells to make tumors more susceptible to cancer immunotherapy.In this regard,we designed and constructed a novel multifunctional nanocomposite comprised of oxidized bacterial cellulose(OBC),thrombin(TB),and gold nanocages(AuNCs)containing anti-programmed death 1(PD-1)antibody(αPD-1@AuNCs),which allows the combination of therapies with remarkable postoperative antitumor immunity to control local tumor recurrence.TheαPD-1@AuNCs displayed high light-to-heat conversion efficiency and induced pyroptosis under near infrared(NIR)irradiation,which activated a potent antitumor immune response.More importantly,the therapeutic system could induce tumor pyroptosis and enhance antitumor immune response by increasing T-cell infiltration and reducing the immune suppressive cells,when combined with local ICB therapy,which effectively avoided the tumor recurrence in a HNSCC postoperative mice model.Overall,the newly developed multifunctional nanocomposites could be a promising candidate for the treatment of postoperative HNSCC.展开更多
With the increasing demand for beauty and health,clear aligners(CAs)have been widely applied among patients with malocclusion.However,patients treated with CAs also face some potential complications,such as deminerali...With the increasing demand for beauty and health,clear aligners(CAs)have been widely applied among patients with malocclusion.However,patients treated with CAs also face some potential complications,such as demineralization,dental caries,and periodontal diseases.In addition,some patients have additional needs to improve their quality of life,such as bleaching teeth.In order to prevent or solve these problems,the modification of CAs is a promising method because their extensive long-term contact with tooth surfaces makes them ideal devices for implementing adjuvant medical functions.In this review,we discuss various advanced CAs with medical functions based on the clinical needs of patients.As far as we know,the additional functions of CAs mainly include antibacterial,remineralization,whitening,and accelerating tooth movement.These functions are achieved by two major pathways,the combination of CAs with drugs/biomaterials and increasing the capacity or affinity of drugs.In addition,we discuss the current limitations of in vitro experiments which are designed to explore the effectiveness and properties of novel CAs,and the challenges of bringing a multifunctional appliance from proposal to clinical application.At the end of this review,we provide insights into the broader prospects for the improvement of CAs.展开更多
基金supported by the National Natural Science Foundation of China(Nos.82222035 and 81602489)the Guangdong Basic and Applied Basic Research Foundation(Nos.2021A1515111036 and 2022A1515110308)+1 种基金the Medical Scientific Research Foundation of Guangdong Province of China(No.A2023274)the Basic Research Program of Shenzhen Innovation Council(No.JCYJ20210324105609024)。
文摘As a type of new carbon-based nanomaterials,carbon dots(CDs)possess exceptional optical properties,making them highly desirable for use in fluorescent sensors.However,the CDs with deep-red(DR)or near-infrared(NIR)emission have rarely been reported.In this work,we prepared deep-red emissive fluorine-doped carbon quantum dots(F-CDs)by introducing a precursor simultaneously containing fluorine and amidogen.The synergistic effect of nitrogen doping and D-π-A pattern production contributed to the maximum emission of F-CDs at 636 nm with an absolute quantum yield of 36.00%±0.68%.Moreover,we designed an F-CDs-based fluorescence assay to determine the content of hypochlorite(ClO^(-)),with a limit of detection(LOD)as low as 15.4 nmol/L,indicating the high sensitivity of F-CDs to ClO^(-).In real samples,the F-CDs-based fluorescent sensor exhibited excellent sensitivity and selectivity in the detection of ClO^(-),with an error below 2%,suggesting their great potential in daily life.In cancer cell imaging,the F-CDs not only demonstrated high sensitivity to ClO^(-)but also exhibited excellent mitochondria targeting,as evidenced by the high Pearson's correlation coefficient(PCC)of 0.93 in colocalization analysis.The work presented here suggests the great potential of replacing commercial dyes with F-CDs for highly specific mitochondria labeling and cell imaging.
基金supported by the Fundamental Research Funds for the Central Universities(Wuhan University,Clinical Medicine+X,2042024YXB017)Hubei Province Chinese Medicine Research Project(ZY2023Q015)+1 种基金Natural Science Foundation of Hubei Province(2023AFB665)Medical Young Talents Program of Hubei Province,Wuhan Young Medical Talents Training Project to L-L.B.
文摘Dental,oral,and craniofacial diseases can substantially impact the quality of human life,thereby posing a serious public health concern.Although conventional therapies such as surgery have solved these problems largely,the prognosis of patients is not always satisfactory.Cell membrane-coated nanoparticles(CMCNPs)carry nanodrugs with the help of natural cell membranes,therefore utilizing their remarkable ability to interface and interact with their surrounding environment.These nanoparticles have demonstrated substantial advantages in drug targeting,prolonging blood circulation time,penetrating biofilms,and immune escape.With the assistance of CMCNPs,the therapeutic effects of dental,oral,and craniofacial diseases can reach a higher level.CMCNPs have been applied for dental,oral,and craniofacial diseases for various conditions such as head and neck cancer,periodontal disease,and oral biosignal detection.For the therapies of head and neck cancer,CMCNPs have been widely utilized as a tool of chemotherapy,phototherapy,and immunotherapy,while yet to be exploited in imaging technique.In the end,we summarized the challenges and prospectives of CMCNPs for dental,oral,and craniofacial diseases:large-scale production with uniform standards and high quantity,extensive application directions in dental,oral,and craniofacial regions(implant,endodontics),and the promotion of its clinical application.
基金support from the National Key Research and Development Program of China(No.2022YFD1800900)the Fundamental Research Funds for the Central Universities(No.2662024HXPY002)+2 种基金State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources(No.SKLCUSA-b202301)the Hubei Province Chinese Medicine Research Project(No.ZY2025Q026)the Medical Young Talents Program of Hubei Province to L.-L.B.
文摘Due to its complex pathogenesis involving dysregulated reactive oxygen species(ROS)storms,cytokine hyperactivation and immune cell infiltration,acute lung injury(ALI)presents critical clinical challenges,and conventional therapies often fail to address the dual requirements of precise inflammatory targeting and microenvironment regulation.Herein,we report an intelligent biomimetic nanosystem(CeGM)featuring(i)synergistic catalytic circuits,where hollow CeO_(2)nanoenzymes are combined with glycyrrhizic acid(GA)through density functional theory(DFT)-optimized binding configurations,achieving charge redistribution-induced dual-site catalytic activation relative to conventional CeO_(2)alone;(ii)pathology-responsive delivery,where macrophage membrane camouflage enables lipopolysaccharide(LPS)recognition and inflammatory chemotaxis,yielding 7.8-fold higher alveolar accumulation than non-coated counterparts in LPS-induced ALI models;(iii)multidimensional immunomodulation,where NOD-like receptor family pyrin domain containing 3(NLRP3)inflammasome activation(56.20%interleukin-1β(IL-1β)reduction)and M1 macrophage polarization(2.4-fold M2/M1 ratio increase)are concurrently suppressed through GA-mediated nuclear factor kappa B(NF-κB)pathway inhibition and CeO_(2)-mediated redox homeostasis restoration.This dual-functional nanoplatform demonstrates favourable therapeutic outcomes in mitigating pulmonary edema by clearing ROS(47.20%reduction in ROS levels),neutrophil infiltration(58.16%myeloperoxidase(MPO)activity decrease)and cytokine storms(tumor necrosis factor-alpha(TNF-α)reduction of 45.90%),offering a paradigm-shifting strategy for precision nanomedicine in acute inflammatory disorders.
基金supported by the National Natural Science Foundation of China(81702703)the China Postdoctoral Science Foundation(2018M630883)+1 种基金the Key Research and Development Program of Science and Technology Department of Sichuan Province(2020YFS0570,2019YFS0514,2022YFS0159,2022YFS0606)the Joint Funds of Center for Engineering Medicine of UESTC(ZYGX2021YGLH017).
文摘In a recent study published in Science,Tsvetkov et al1.shed a light on a new form of cell death,copper-dependent cell death(termed cuproptosis).They defined“cuproptosis”as a nonapoptotic cell death pathway.They have demonstrated that copper directly binds to lipoylated components of the tricarboxylic acid(TCA)cycle.Then aggregation of these copper-bound,lipoylated mitochondrial proteins and subsequent Fe-S cluster protein loss triggered proteotoxic stress and a distinct form of cell death.
基金supported by China Postdoctoral Science Foundation(2018M630883 and 2019T120688)Hubei Province Chinese Medicine Research Project(ZY2023Q015)+2 种基金Natural Science Foundation of China(61904057 and 22193051)Hubei Province(2023AFB665 and 2018CFB124)Fundamental Research Funds for the Central Universities(Wuhan University,Clinical Medicine+X,2042024YXB017),Medical Young Talents Program of Hubei Province and Wuhan Young Medical Talents Training Project.
文摘Dental,oral,and craniofacial diseases jeopardize health and reduce the quality of life.Accessing disease-related signals in advance is beneficial to prevent the occurrence or progression of those diseases.However,the inconvenience of periodical in-hospital examinations and the difficulty of sustaining daily health monitoring challenge personal compliance and possibly lead to limited prevention or treatment.Medical flexible electronics are electric devices fabricated on soft and extensible substrates to fit the human skin and enable non-invasive continuous monitoring of biophysical/biochemical signals.They provide the possibility of long-term,continuous,comfortable,and wireless healthcare monitoring and are expected to alleviate time and economic consumption by avoiding in-hospital examinations and treatment.Therefore,flexible electronics have emerged for early diagnosis and disease monitoring in stomatology.It is noteworthy that special biophysical/biochemical characteristics and the environment of dental,oral,and craniofacial areas bring distinct challenges that flexible electronics need to address ingeniously to ensure their stability,selectivity,and sensitivity.This review summaries flexible electronics and their specificity when used in dental,oral,and craniofacial applications,including monitoring saliva or cavity-gas related biosignals,sensing the mechanical fluctuation from facial muscle/respiratory activities or orthodontic forces,and executing special functions in the prevention or postoperative recovery of relevant diseases.Furthermore,after analyzing current challenges and proposing potential solutions,the“5I”principles of imperceptibility,intelligence,individualization,integration,and inexpensiveness are presented to help guide the future development of flexible electronics and promote their commercialization for dental,oral,and craniofacial medicine.
基金This work was supported by the National Natural Science Foundation of China(Nos.82072996(Z.J.S.)81874131(Z.J.S.)+1 种基金81702730(L.L.B.),and 51973076(G.Y.))the Fundamental Research Funds for the Central Universities(No.2042021kf0216)to Z.J.S.,China Postdoctoral Science Foundation(Nos.2018M630883 and 2019T120688)to L.L.B.,and Wuhan Young Medical Talents Training Project to L.L.B.
文摘The recurrence of head and neck squamous cell carcinoma(HNSCC)after surgical resection continues to pose a major challenge to cancer treatment.Advanced HNSCC exhibits a low response rate to immune checkpoint blockade(ICB),while photothermal therapy(PTT)can increase the infiltration of immune cells to make tumors more susceptible to cancer immunotherapy.In this regard,we designed and constructed a novel multifunctional nanocomposite comprised of oxidized bacterial cellulose(OBC),thrombin(TB),and gold nanocages(AuNCs)containing anti-programmed death 1(PD-1)antibody(αPD-1@AuNCs),which allows the combination of therapies with remarkable postoperative antitumor immunity to control local tumor recurrence.TheαPD-1@AuNCs displayed high light-to-heat conversion efficiency and induced pyroptosis under near infrared(NIR)irradiation,which activated a potent antitumor immune response.More importantly,the therapeutic system could induce tumor pyroptosis and enhance antitumor immune response by increasing T-cell infiltration and reducing the immune suppressive cells,when combined with local ICB therapy,which effectively avoided the tumor recurrence in a HNSCC postoperative mice model.Overall,the newly developed multifunctional nanocomposites could be a promising candidate for the treatment of postoperative HNSCC.
基金supported by Postdoctoral Science Foundation of China(Nos.2018M630883 and 2019T120688)Hubei Province Chinese Medicine Research Project(No.ZY2023Q015)Natural Science Foundation of Hubei Province(No.2023AFB665)。
文摘With the increasing demand for beauty and health,clear aligners(CAs)have been widely applied among patients with malocclusion.However,patients treated with CAs also face some potential complications,such as demineralization,dental caries,and periodontal diseases.In addition,some patients have additional needs to improve their quality of life,such as bleaching teeth.In order to prevent or solve these problems,the modification of CAs is a promising method because their extensive long-term contact with tooth surfaces makes them ideal devices for implementing adjuvant medical functions.In this review,we discuss various advanced CAs with medical functions based on the clinical needs of patients.As far as we know,the additional functions of CAs mainly include antibacterial,remineralization,whitening,and accelerating tooth movement.These functions are achieved by two major pathways,the combination of CAs with drugs/biomaterials and increasing the capacity or affinity of drugs.In addition,we discuss the current limitations of in vitro experiments which are designed to explore the effectiveness and properties of novel CAs,and the challenges of bringing a multifunctional appliance from proposal to clinical application.At the end of this review,we provide insights into the broader prospects for the improvement of CAs.
