Photomodulation technology,characterized by its high spatiotemporal resolution,has emerged as a transformative approach for precise,rapid,and noninvasive regulation of intricate cellular signaling networks,offering un...Photomodulation technology,characterized by its high spatiotemporal resolution,has emerged as a transformative approach for precise,rapid,and noninvasive regulation of intricate cellular signaling networks,offering unprecedented opportunities for biomedical research.Nevertheless,the in vivo implementation of wireless photomodulation remains constrained by the inherent limitations of conventional photoresponsive systems.Addressing this challenge,we report a BODIPY-based photocatalyst with exceptional red-light responsiveness(λ>630 nm).Transient spectroscopic studies show that this photocatalyst exhibits the feature with solvent polarity-switching excited state dynamics.In addition,we observed ultralong triplet-state lifetimes(590μs in tetrahydrofuran;862μs in toluene),which is favorable for establishing a far-red-light-driven photocatalytic decaging platform that synergistically integrates the BODIPY photocatalyst with endogenous NADH as the intrinsic electron donor.Crucially,this system demonstrates robust in vivo efficacy,achieving significant tumor growth suppression in murine tumor models through localized prodrug activation.This work not only provides fundamental insights into engineering long-lived triplet states in metal-free organic photocatalysts but also pioneers a biocompatible strategy for spatiotemporally controlled therapeutic interventions,bridging the gap between advanced photochemistry and precision biomedicine.展开更多
The growth and metastasis of malignant solid tumors depend closely on newblood vessels. Vasculogenic mimicry provides a special pathway of blood supply during theearly growth of malignant tumors, and real-time monitor...The growth and metastasis of malignant solid tumors depend closely on newblood vessels. Vasculogenic mimicry provides a special pathway of blood supply during theearly growth of malignant tumors, and real-time monitoring of its occurrence anddevelopment in vivo is important to clinical applications. However, there are few labelswith sufffcient brightness and stability in vivo to achieve high spatiotemporal resolutionimaging of deep tissue for noninvasive optical detection of vasculogenic mimicry in tumortissues. In this study, we constructed a high-brightness ffuorescent label with ffuorescence inthe near-infrared-II region, which can be used not only for in vivo tumor imaging but also fortissue section imaging. Real-time high-resolution imaging of tumor vessels has been achievedwith PbS quantum dots (QDs) surface-coupled with horseradish peroxidase (HRP) (HRPQDs) by taking advantage of the low background autoffuorescence of tissue at the nearinfrared-II wavelength for in vivo and tissue section imaging. Qualitative and quantitativeanalysis of early blood supply patterns of tumor growth enables monitoring neovascularizationto accurate noninvasive identiffcation of benign and malignant solid tumors.展开更多
基金supported by the National Natural Science Foundation of China(NSFC)(013398,22377063)the Research Start-Up Fund of Nankai University and the Haihe Laboratory of Sustainable Chemical Transformations(24HHWCSS00020)。
文摘Photomodulation technology,characterized by its high spatiotemporal resolution,has emerged as a transformative approach for precise,rapid,and noninvasive regulation of intricate cellular signaling networks,offering unprecedented opportunities for biomedical research.Nevertheless,the in vivo implementation of wireless photomodulation remains constrained by the inherent limitations of conventional photoresponsive systems.Addressing this challenge,we report a BODIPY-based photocatalyst with exceptional red-light responsiveness(λ>630 nm).Transient spectroscopic studies show that this photocatalyst exhibits the feature with solvent polarity-switching excited state dynamics.In addition,we observed ultralong triplet-state lifetimes(590μs in tetrahydrofuran;862μs in toluene),which is favorable for establishing a far-red-light-driven photocatalytic decaging platform that synergistically integrates the BODIPY photocatalyst with endogenous NADH as the intrinsic electron donor.Crucially,this system demonstrates robust in vivo efficacy,achieving significant tumor growth suppression in murine tumor models through localized prodrug activation.This work not only provides fundamental insights into engineering long-lived triplet states in metal-free organic photocatalysts but also pioneers a biocompatible strategy for spatiotemporally controlled therapeutic interventions,bridging the gap between advanced photochemistry and precision biomedicine.
文摘The growth and metastasis of malignant solid tumors depend closely on newblood vessels. Vasculogenic mimicry provides a special pathway of blood supply during theearly growth of malignant tumors, and real-time monitoring of its occurrence anddevelopment in vivo is important to clinical applications. However, there are few labelswith sufffcient brightness and stability in vivo to achieve high spatiotemporal resolutionimaging of deep tissue for noninvasive optical detection of vasculogenic mimicry in tumortissues. In this study, we constructed a high-brightness ffuorescent label with ffuorescence inthe near-infrared-II region, which can be used not only for in vivo tumor imaging but also fortissue section imaging. Real-time high-resolution imaging of tumor vessels has been achievedwith PbS quantum dots (QDs) surface-coupled with horseradish peroxidase (HRP) (HRPQDs) by taking advantage of the low background autoffuorescence of tissue at the nearinfrared-II wavelength for in vivo and tissue section imaging. Qualitative and quantitativeanalysis of early blood supply patterns of tumor growth enables monitoring neovascularizationto accurate noninvasive identiffcation of benign and malignant solid tumors.
