Two new triterpenoid saponins named asterbatanoside H and I have been isolated from the roots of Aster batsngensis and their structures elucidated as 3-O-β-D-glucopyranosyl-(1→3) β-D-glucopyranosyl-bayogenin-28-O-...Two new triterpenoid saponins named asterbatanoside H and I have been isolated from the roots of Aster batsngensis and their structures elucidated as 3-O-β-D-glucopyranosyl-(1→3) β-D-glucopyranosyl-bayogenin-28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosy-(1→6) and 3-O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-23-O-acetyl-bayogenin-28-O-β-D-glucopyranosyl-(1→6) β-D-glucopyranoside, respectively, by means of spectral and chemical data.展开更多
A new medicagenic acid saponin named asterbatanoside J was isolated from the roots of Aster batangensis. On the basis of chemical and spectral studies especially 2D NMR including COSY, HETCOR, HOHAHA, ROESY and HMBC t...A new medicagenic acid saponin named asterbatanoside J was isolated from the roots of Aster batangensis. On the basis of chemical and spectral studies especially 2D NMR including COSY, HETCOR, HOHAHA, ROESY and HMBC techniques, its structure has been established as 3-O-beta -D-glucopyranosyl-(1 -->6)-beta -D-glucopyranosyl-2 beta ,3 beta -dihydroxy-olean-12-en-23 alpha ,28-dioic acid-28-O-alpha -L-arabinopyranosyl-(1 -->3)-alpha -L-rhamnopyranosyl-(1 -->2)-beta -D-fucopyranoside.展开更多
THE tendency of the technique of spectral measurement is now towards sensitivity, precision,quickness, time and spatial resolving spectrum. For the spectrophotometers, which acquirespectral data with the serious metho...THE tendency of the technique of spectral measurement is now towards sensitivity, precision,quickness, time and spatial resolving spectrum. For the spectrophotometers, which acquirespectral data with the serious method, the measurement of spectral intensity for differentwavelengths is not simultaneous. It is difficult to quickly get a spectrum, because time must bespent on tuning the dispersive device, and it is also impossible to process time and spatial Te-solving spectrum. The problems mentioned above are the fatal disadvantages for further devel-oping the spectrophotometer equipped with monochromator. The best way to solve these prob-展开更多
To understand the molecular mechanism of TNFα effects, the cDNA of murine BRI3 gene was cloned from the total RNA of murine brain endothelial cells (bEnd.3) treated with hTNFa by using the suppression subtractive hyb...To understand the molecular mechanism of TNFα effects, the cDNA of murine BRI3 gene was cloned from the total RNA of murine brain endothelial cells (bEnd.3) treated with hTNFa by using the suppression subtractive hybridization (SSH) and the RT-PCR method. The fusion expression vector harbouring BRI3 gene and enhanced green fluorescence protein (EGFP) thus obtained were designated as pEGFP/I3. Then pEGFP/I3 was transiently transfected into L929 cells and the fusion protein EGFP/I3 was localized in cytoplasm. It is found that the expression of EGFP/I3 could induce cell death in L929 cells detected by TUNEL method and flow cytometry. And the overexpression of Bcl-2 in L929 cells can block cell death induced by EGFP/I3, indicating that murine BRI3 gene might related to the TNFα mediated cytotoxicity.展开更多
文摘Two new triterpenoid saponins named asterbatanoside H and I have been isolated from the roots of Aster batsngensis and their structures elucidated as 3-O-β-D-glucopyranosyl-(1→3) β-D-glucopyranosyl-bayogenin-28-O-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosy-(1→6) and 3-O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-23-O-acetyl-bayogenin-28-O-β-D-glucopyranosyl-(1→6) β-D-glucopyranoside, respectively, by means of spectral and chemical data.
文摘A new medicagenic acid saponin named asterbatanoside J was isolated from the roots of Aster batangensis. On the basis of chemical and spectral studies especially 2D NMR including COSY, HETCOR, HOHAHA, ROESY and HMBC techniques, its structure has been established as 3-O-beta -D-glucopyranosyl-(1 -->6)-beta -D-glucopyranosyl-2 beta ,3 beta -dihydroxy-olean-12-en-23 alpha ,28-dioic acid-28-O-alpha -L-arabinopyranosyl-(1 -->3)-alpha -L-rhamnopyranosyl-(1 -->2)-beta -D-fucopyranoside.
文摘THE tendency of the technique of spectral measurement is now towards sensitivity, precision,quickness, time and spatial resolving spectrum. For the spectrophotometers, which acquirespectral data with the serious method, the measurement of spectral intensity for differentwavelengths is not simultaneous. It is difficult to quickly get a spectrum, because time must bespent on tuning the dispersive device, and it is also impossible to process time and spatial Te-solving spectrum. The problems mentioned above are the fatal disadvantages for further devel-oping the spectrophotometer equipped with monochromator. The best way to solve these prob-
基金This work was supported by the National Climbing Plan and the Shanghai-Unilever Research and Development Fund (Grant No. 9909).
文摘To understand the molecular mechanism of TNFα effects, the cDNA of murine BRI3 gene was cloned from the total RNA of murine brain endothelial cells (bEnd.3) treated with hTNFa by using the suppression subtractive hybridization (SSH) and the RT-PCR method. The fusion expression vector harbouring BRI3 gene and enhanced green fluorescence protein (EGFP) thus obtained were designated as pEGFP/I3. Then pEGFP/I3 was transiently transfected into L929 cells and the fusion protein EGFP/I3 was localized in cytoplasm. It is found that the expression of EGFP/I3 could induce cell death in L929 cells detected by TUNEL method and flow cytometry. And the overexpression of Bcl-2 in L929 cells can block cell death induced by EGFP/I3, indicating that murine BRI3 gene might related to the TNFα mediated cytotoxicity.
