Alzheimer’s disease(AD)is a neurodegenerative disorder associated with brain aging,and the accumulation ofβ-amyloid(Aβ)and hyperphosphorylated Tau proteins are key pathological features.Currently,drugs for the trea...Alzheimer’s disease(AD)is a neurodegenerative disorder associated with brain aging,and the accumulation ofβ-amyloid(Aβ)and hyperphosphorylated Tau proteins are key pathological features.Currently,drugs for the treatment of AD are mainly single-targeted,but the complex pathogenesis of AD makes it difficult to achieve the desired results.Therefore,the development of multitargeted therapies is crucial for future interventions.Rice bran oil(RBO)has been recognized as an edible oil with several health benefits,but its effects on AD caused by brain aging remain underexplored.In this study,the effects of RBO on memory dysfunction in D-galactose(D-gal)mice and its molecular mechanisms were investigated via in vivo and in silico methods from the perspective of AD pathologies.Our results suggested that compounds in RBO could modulate the activities of Aβprecursor protein cleaving enzyme 1(BACE1),mitogen-activated protein kinase 3(MAPK3),matrix metalloproteinase 3(MMP3),and intercellular adhesion molecule 1(ICAM1),leading to inhibition of Aβaccumulation and Tau protein hyperphosphorylation.Moreover,RBO reduced Aβ-induced oxidative stress by inhibiting the activity of mouse double minute 2 homolog(MDM2)and cyclic adenosine monophosphate(cAMP)response element binding protein binding protein(CREBBP),and attenuated neuroinflammation by inhibiting the activity of nitric oxide synthase 2(NOS2)and reducing Aβaccumulation and Tau protein hyperphosphorylation.Additionally,α-linolenic acid in RBO exhibited inhibitory effects on D-gal-induced apoptosis in PC12 cells through modulation of NOS2,MDM2,ICAM1,and phospho-extracellular signal-regulated kinase 1/2(p-ERK1/2).Similarly,stigmastanol inhibited apoptosis in D-gal-induced PC12 cells through the regulation of NOS2.Thus,RBO can be considered as a potential functional food to attenuate AD owing to its multicomponent and multitarget effects.展开更多
BACKGROUND Diabetic foot ulcers(DFUs)present a significant healthcare challenge attributable to their high rates of disability and the limitations of applied traditional nursing approaches.Effective management is crit...BACKGROUND Diabetic foot ulcers(DFUs)present a significant healthcare challenge attributable to their high rates of disability and the limitations of applied traditional nursing approaches.Effective management is critical for uneventful health outcomes.AIM To investigate the effects of multidisciplinary team(MDT)nursing interventions and blood glucose control on the negative emotions and satisfaction of DFUhealing patients.METHODS This retrospective cohort study included 115 patients with DFUs,divided into MDT and blood glucose control intervention group(n=60)and standard care control group(n=55).The comparison factors were wound area,new granulation tissue coverage area,wound healing rate,2-hour postprandial blood glucose level,fasting plasma glucose level,Hamilton Anxiety Scale score,Hamilton Depression Scale score,and nursing satisfaction.RESULTS After 4 weeks,the average wound area reduced from 22.04±6.48 cm^(2)to 11.96±3.63 cm^(2)(P<0.05).New granulation tissue coverage area reached 52.85±18.39 cm^(2) for the intervention group and 28.39±9.94 cm^(2)(P<0.05)in the control group,respectively.The healing rate was significantly higher in the intervention group than in the control group(91.7%vs 76.4%,P<0.05).Fasting plasma glucose decreased more sharply in the intervention group(from 8.36±0.98 mmol/L to 6.91±1.23 mmol/L)than in the control group(8.41±1.05 mmol/L to 7.81±1.27 mmol/L),with the intervention group maintaining significantly lower levels(P<0.05).The intervention group demonstrated a significantly greater reduction in 2-hour postprandial blood glucose levels(11.35±2.67 mmol/L to 7.52±1.38 mmol/L)compared to the control group(11.61±3.01 mmol/L to 8.72±1.63 mmol/L;P<0.05).Hamilton Anxiety Scale and Hamilton Depression Scale scores were significantly lower in the intervention group(P<0.05).Patient satisfaction with nursing was 93.33%and 74.55%in the intervention and control groups,respectively(P<0.05).CONCLUSION MDT combined with blood glucose control enhanced healing rates and positively influenced emotional well-being and satisfaction among patients.This strategy holds potential for application in clinical practice.展开更多
Background Chronic heat stress(CHS)is a detrimental environmental stressor with a negative impact on the meat quality of broilers.However,the underlying mechanisms are not fully understood.This study investigates the ...Background Chronic heat stress(CHS)is a detrimental environmental stressor with a negative impact on the meat quality of broilers.However,the underlying mechanisms are not fully understood.This study investigates the effects of CHS on long non-coding RNA(lncRNA)expression and muscle injury in broilers,with a focus on its implications for meat quality.Results The results showed that CHS diminished breast muscle yield,elevated abdominal fat deposition,induced cellular apoptosis(P<0.05),and caused myofibrosis.Transcriptomic analysis revealed 151 differentially expressed(DE)lncRNAs when comparing the normal control(NC)and HS groups,214 DE lncRNAs when comparing the HS and PF groups,and 79 DE lncRNAs when comparing the NC and pair-fed(PF)groups.After eliminating the confounding effect of feed intake,68 lncRNAs were identified,primarily associated with cellular growth and death,signal transduc-tion,and metabolic regulation.Notably,the apoptosis-related pathway P53,lysosomes,and the fibrosis-related gene TGF-β2 were significantly upregulated by lncRNAs.Conclusions These findings indicate that chronic heat stress induces cellular apoptosis and muscle injury through lncRNA,leading to connective tissue accumulation,which likely contributes to reduced breast muscle yield and meat quality in broilers.展开更多
Wintersweet(Chimonanthus praecox),a well-known fragrant flowering shrub,is extensively planted for ornamental purpose and production of floral essential oil.Although the tepal color of wintersweet varieties exhibits t...Wintersweet(Chimonanthus praecox),a well-known fragrant flowering shrub,is extensively planted for ornamental purpose and production of floral essential oil.Although the tepal color of wintersweet varieties exhibits the most remarkable diversity,variations in the floral scent traits are also noticeable across different cultivars.In this study,the floral volatile organic compounds(VOCs)in three wintersweet cultivars,‘Yanlingsuxin’,‘Yuxiang’,and‘Hongyun’were detected via GC-MS coupled with OAV.The distinct floral aromas of the three cultivars were primarily attributed to benzyl alcohol(abundant in‘Yuxiang’),linalool(abundant in‘Yanlingsuxin’),(-)-γ-cadinene and eugenol(abundant in‘Hongyun’).Integrated analyses of metabolome and transcriptome showed that an R2R3-MYB transcription factor gene,CpODO1,potentially have a crucial regulatory function in controlling the production of multiple aroma compounds.Overexpression of CpODO1 can enhance the production of benzyl alcohol in transgenic tobacco flowers.Analysis of DAP-seq data,EMSA and dual-luciferase assay revealed that CpODO1 predominantly regulate the expression of CpCYP71,a cytochrome P450 gene encoding a key enzyme in the production of benzyl alcohol,and the transcriptional regulation of CpODO1 is driven by CpEOBII.The identification of polymorphisms in the MYB binding cis-motifs of CpCYP71 and CpODO1 promoters revealed the regulatory mechanism underlying the varied synthesis of benzyl alcohol in three wintersweet cultivars.This study provides new anchor points for floral scent quality improvement breeding of wintersweet,and the profusion of wintersweet germplasm can serve as a material basis for developing various aroma products.展开更多
The Hydro-Viscous Drive(HVD)speed regulating system finds extensive application in air transport transmission systems to regulate the stepless speed or conduct overload protection.However,its intrinsic hysteretic beha...The Hydro-Viscous Drive(HVD)speed regulating system finds extensive application in air transport transmission systems to regulate the stepless speed or conduct overload protection.However,its intrinsic hysteretic behaviors,such as the asymmetric hysteretic and dead zone,could introduce inaccuracy and delay in control applications,posing challenges to system regulation.This paper investigates a Nonlinear Hysteresis Compensation Control(NHCC)that consists of two parts to control the HVD output speed by operating the valve under different engine operating conditions.In the first part,the Inverse Hysteresis Compensator(IHC)based on major loop data is introduced for the asymmetric hysteresis characterization and compensation of the HVD speed control system of the power generation and distribution,which aims to reduce the hysteresis and dead zone effect and expand the effective input range.In the second part,the Active Disturbance Rejection Controller(ADRC)is employed to mitigate the hysteresis effects of the compensated system and remove the steady-state error,which allows real-time compensation of the estimated perturbations as state feedback to achieve the required performance.An experimental laboratory station has been fabricated to evaluate the proposed method.The test results show that the NHCC method can regulate the fan speed to the desired value(45 r/min at steady state)and broaden the effective input range to the full range under different engine conditions.Besides,the proposed control method can reduce the non-linearity of the input and output curves(from 18%to 4%)and compensate for the asymmetric hysteresis(from 38%to 5%).展开更多
Gastrointestinal(GI)cancers are prevalent globally,with leading incidence and mortality rates among malignant tumors.Despite notable advancements in surgical resection,radiotherapy,and chemotherapy,the overall surviva...Gastrointestinal(GI)cancers are prevalent globally,with leading incidence and mortality rates among malignant tumors.Despite notable advancements in surgical resection,radiotherapy,and chemotherapy,the overall survival rates remain low.Hence,it is imperative to explore alternative approaches that enhance patient outcomes.Cluster of differentiation 47(CD47),serving as an early diagnostic marker,is predominantly overexpressed in GI cancers and associated with poor prognosis.Targeting the CD47-signal regulatory protein alpha(SIRPa)signaling pathway may provide a novel strategy for GI cancers treatment.This study summarizes current knowledge of the structure and function of CD47 and SIRPa,their roles in signaling pathways,the prognostic significance of CD47,therapeutic strategies targeting the CD47-SIRPα signaling pathway in GI cancer,and highlights key issues for future investigations.展开更多
AIM: To determine the possible predisposing factors of bezoar-induced small bowel obstruction(b I-Sb O) and to discuss the diagnostic value of multi-slice spiral computed tomography,particularly contrast-enhanced scan...AIM: To determine the possible predisposing factors of bezoar-induced small bowel obstruction(b I-Sb O) and to discuss the diagnostic value of multi-slice spiral computed tomography,particularly contrast-enhanced scanning,in this condition.METHODS: A total of 35 b I-Sb O cases treated at our hospital from January 2007 to December 2013 were retrospectively analysed.Complete clinical and computed tomography(CT) data of the patients were available and confirmed by surgery.SbO was clinically diagnosed on the basis of clinical manifestations.Of the 35 patients,18 underwent abdominal and pelvic CT planar scanning with GE 64-slice spiral CT and 17 underwent abdominal and pelvic CT planar scanning with GE 64-slice spiral CT combined with contrastenhanced examination.Original images were processed using a GE ADW4.3 workstation to obtain MPR,CPR,MIP and CTA images.The images of all patients were evaluated by two abdominal imaging experts.The main analytical contents of planar scanning included intestinal bezoar conditions,changes in the intestinal wall and changes in peri-intestinal conditions.Vascular hyperaemia and arterial blood supply conditions at a specific obstruction site and the distal end of the obstruction site were evaluated through contrastenhanced examination.RESULTS: The proportion of males to females among the 35 cases was 1:1.69(13:22); median age was 63.3 years.The following cases were observed: 29(82.8%) cases occurred in autumn and winter and showed a history of consuming high amounts of persimmon and hawthorn; 19(54.3%) cases revealed a history of gastrointestinal surgery; 19 exhibited incomplete dentition,with missing partial or whole posterior teeth; 26 suffered from obstruction at the ileum.A total of 51 bezoars were found in these patients,of whom 16(45.7%) had multiple bezoars.CT planar scanning of bezoars showed lumps with mottled gas inside the intestinal cavity.Furthermore,9 cases of bezoars had envelopes and 11 cases were accompanied with thickening of the distal wall of the obstructed bowel.Scanning of 17 cases was enhanced; the results revealed that the mesenteric blood vessels at the obstruction site and the proximal site were dilated,and a total of 7 cases were accompanied with distal vascular dilation and intestinal wall thickening.CONCLUSION: b I-Sb O exhibits regional and seasonal characteristics.CT planar and contrast-enhanced scanning can be applied to diagnose and observe vascular conditions in obstructed zones.展开更多
The recycling and reutilization of spent lithium-ion batteries(LIBs)have become an important measure to alleviate problems like resource scarcity and environmental pollution.Although some progress has been made,batter...The recycling and reutilization of spent lithium-ion batteries(LIBs)have become an important measure to alleviate problems like resource scarcity and environmental pollution.Although some progress has been made,battery recycling technology still faces challenges in terms of efficiency,effectiveness and environmental sustainability.This review aims to systematically review and analyze the current status of spent LIB recycling,and conduct a detailed comparison and evaluation of different recycling processes.In addition,this review introduces emerging recycling techniques,including deep eutectic solvents,molten salt roasting,and direct regeneration,with the intent of enhancing recycling efficiency and diminishing environmental repercussions.Furthermore,to increase the added value of recycled materials,this review proposes the concept of upgrading recycled materials into high value-added functional materials,such as catalysts,adsorbents,and graphene.Through life cycle assessment,the paper also explores the economic and environmental impacts of current battery recycling and highlights the importance that future recycling technologies should achieve a balance between recycling efficiency,economics and environmental benefits.Finally,this review outlines the opportunities and challenges of recycling key materials for next-generation batteries,and proposes relevant policy recommendations to promote the green and sustainable development of batteries,circular economy,and ecological civilization.展开更多
Hypoxia,as an important hallmark of the tumor microenvironment,is a major cause of oxidative stress and plays a central role in various malignant tumors,including glioblastoma.Elevated reactive oxygen species(ROS)in a...Hypoxia,as an important hallmark of the tumor microenvironment,is a major cause of oxidative stress and plays a central role in various malignant tumors,including glioblastoma.Elevated reactive oxygen species(ROS)in a hypoxic microenvironment promote glioblastoma progression;however,the underlying mechanism has not been clarified.Herein,we found that hypoxia promoted ROS production,and the proliferation,migration,and invasion of glioblastoma cells,while this promotion was restrained by ROS scavengers N-acetyl-L-cysteine(NAC)and diphenyleneiodonium chloride(DPI).Hypoxia-induced ROS activated hypoxia-inducible factor-1α(HIF-1α)signaling,which enhanced cell migration and invasion by epithelial-mesenchymal transition(EMT).Furthermore,the induction of serine protease inhibitor family E member 1(SERPINE1)was ROS-dependent under hypoxia,and HIF-1αmediated SERPINE1 increase induced by ROS via binding to the SERPINE1 promoter region,thereby facilitating glioblastoma migration and invasion.Taken together,our data revealed that hypoxia-induced ROS reinforce the hypoxic adaptation of glioblastoma by driving the HIF-1α-SERPINE1 signaling pathway,and that targeting ROS may be a promising therapeutic strategy for glioblastoma.展开更多
Semen from 5210 sperm bank donors was analyzed and trends in semen quality were evaluated at Shandong Human Sperm Bank between 2008 and 2014. After 2-7 days of abstinence, semen samples were collected. Measurements of...Semen from 5210 sperm bank donors was analyzed and trends in semen quality were evaluated at Shandong Human Sperm Bank between 2008 and 2014. After 2-7 days of abstinence, semen samples were collected. Measurements of semen volume, sperm concentration, sperm forward motility, and total sperm count were performed. There were significant declining trends in semen volume, sperm concentration, sperm forward motility, and total sperm count. Our results indicate that the quality of semen in this cohort of sperm donors had decreased during the study period.展开更多
Purpose:Interdisciplinarity is a hot topic in science and technology policy.However,the concept of interdisciplinarity is both abstract and complex,and therefore difficult to measure using a single indicator.A variety...Purpose:Interdisciplinarity is a hot topic in science and technology policy.However,the concept of interdisciplinarity is both abstract and complex,and therefore difficult to measure using a single indicator.A variety of metrics for measuring the diversity and interdisciplinarity of articles,journals,and fields have been proposed in the literature.In this article,we ask whether institutions can be ranked in terms of their(inter-)disciplinary diversity.Design/methodology/approach:We developed a software application(interd_vb.exe)that outputs the values of relevant diversity indicators for any document set or network structure.The software is made available,free to the public,online.The indicators it considers include the advanced diversity indicators Rao-Stirling(RS)diversity and DIV*,as well as standard measures of diversity,such as the Gini coefficient,Shannon entropy,and the Simpson Index.As an empirical demonstration of how the application works,we compared the research portfolios of 42“Double First-Class”Chinese universities across Web of Science Subject Categories(WCs).Findings:The empirical results suggest that DIV*provides results that are more in line with one’s intuitive impressions than RS,particularly when the results are based on sampledependent disparity measures.Furthermore,the scores for diversity are more consistent when based on a global disparity matrix than on a local map.Research limitations:“Interdisciplinarity”can be operationalized as bibliographic coupling among(sets of)documents with references to disciplines.At the institutional level,however,diversity may also indicate comprehensiveness.Unlike impact(e.g.citation),diversity and interdisciplinarity are context-specific and therefore provide a second dimension to the evaluation.Policy or practical implications:Operationalization and quantification make it necessary for analysts to make their choices and options clear.Although the equations used to calculate diversity are often mathematically transparent,the specification in terms of computer code helps the analyst to further precision in decisions.Although diversity is not necessarily a goal of universities,a high diversity score may inform potential policies concerning interdisciplinarity at the university level.Originality/value:This article introduces a non-commercial online application to the public domain that allows researchers and policy analysts to measure“diversity”and“interdisciplinarity”using the various indicators as encompassing as possible for any document set or network structure(e.g.a network of co-authors).Insofar as we know,such a professional computing tool for evaluating data sets using diversity indicators has not yet been made available online.展开更多
Birds exhibit extraordinary mobility and remarkable navigational skills,obtaining guidance cues from the Earth’s magnetic field for orientation and long-distance movement.Bird species also show tremendous diversity i...Birds exhibit extraordinary mobility and remarkable navigational skills,obtaining guidance cues from the Earth’s magnetic field for orientation and long-distance movement.Bird species also show tremendous diversity in navigation strategies,with considerable differences even within the same taxa and among individuals from the same population.The highly conserved iron and iron-sulfur cluster binding magnetoreceptor(MagR)protein is suggested to enable animals,including birds,to detect the geomagnetic field and navigate accordingly.Notably,MagR is also implicated in other functions,such as electron transfer and biogenesis of iron-sulfur clusters,raising the question of whether variability exists in its biochemical and biophysical features among species,particularly birds.In the current study,we conducted a comparative analysis of MagR from two different bird species,including the migratory European robin(Erithacus rubecula)and the homing pigeon(Columba livia).Sequence alignment revealed an extremely high degree of similarity between the MagRs of these species,with only three sequence variations.Nevertheless,two of these variations underpinned significant differences in metal binding capacity,oligomeric state,and magnetic properties.These findings offer compelling evidence for the marked differences in MagR between the two avian species,potentially explaining how a highly conserved protein can mediate such diverse functions.展开更多
Glial cell line-derived neurotrophic factor(GDNF)plays an important role in the protection of dopaminergic neurons,but there are few reports of the relationship between GDNF and its precursors(α-pro-GDNF andβ-pro-GD...Glial cell line-derived neurotrophic factor(GDNF)plays an important role in the protection of dopaminergic neurons,but there are few reports of the relationship between GDNF and its precursors(α-pro-GDNF andβ-pro-GDNF)and cognitive impairment in Parkinson’s disease.This study aimed to investigate the relationship between the serum levels of GDNF and its precursors and cognitive impairment in Parkinson’s disease,and to assess their potential as a diagnostic marker.Fifty-three primary outpatients and hospitalized patients with Parkinson’s disease(23 men and 30 women)with an average age of 66.58 years were enrolled from the Affiliated Hospital of Xuzhou Medical University of China in this case-control study.The patients were divided into the Parkinson’s disease with cognitive impairment group(n=27)and the Parkinson’s disease with normal cognitive function group(n=26)based on their Mini-Mental State Examination,Montreal Cognitive Assessment,and Clinical Dementia Rating scores.In addition,26 age-and sex-matched healthy subjects were included as the healthy control group.Results demonstrated that serum GDNF levels were significantly higher in the Parkinson’s disease with normal cognitive function group than in the other two groups.There were no significant differences in GDNF precursor levels among the three groups.Correlation analysis revealed that serum GDNF levels,GDNF/α-pro-GDNF ratios,and GDNF/β-pro-GDNF ratios were moderately or highly correlated with the Mini-Mental State Examination,Montreal Cognitive Assessment,and Clinical Dementia Rating scores.To explore the risk factors for cognitive impairment in patients with Parkinson’s disease,logistic regression analysis and stepwise linear regression analysis were performed.Both GDNF levels and Hoehn-Yahr stage were risk factors for cognitive impairment in Parkinson’s disease,and were the common influencing factors for cognitive scale scores.Neitherα-pro-GDNF norβ-pro-GDNF was risk factors for cognitive impairment in Parkinson’s disease.A receiver operating characteristic curve of GDNF was generated to predict cognitive function in Parkinson’s disease(area under the curve=0.859).This result indicates that the possibility that serum GDNF can correctly distinguish whether patients with Parkinson’s disease have cognitive impairment is 0.859.Together,these results suggest that serum GDNF may be an effective diagnostic marker for cognitive impairment in Parkinson’s disease.However,α-pro-GDNF andβ-pro-GDNF are not useful for predicting cognitive impairment in this disease.This study was approved by Ethics Committee of the Affiliated Hospital of Xuzhou Medical University,China(approval No.XYFY2017-KL047-01)on November 30,2017.展开更多
AIM： To observe the therapeutic effect of corneal collagen cross-linking（CXL） in combination with liposomal amphotericin B in fungal corneal ulcers.METHODS： New Zealand rabbits were induced fungal corneal ulcers b...AIM： To observe the therapeutic effect of corneal collagen cross-linking（CXL） in combination with liposomal amphotericin B in fungal corneal ulcers.METHODS： New Zealand rabbits were induced fungal corneal ulcers by scratching and randomly divided into 3groups, i.e. control, treated with CXL, and combined therapy of CXL with 0.25% liposomal amphotericin B（n =5 each）. The corneal lesions were documented with slit-lamp and confocal microscopy on 3, 7, 14, 21 and 28 d after treatment. The corneas were examined with transmission electron microscopy（TEM） at 4wk.RESULTS： A rabbit corneal ulcer model of Fusarium was successfully established. The corneal epithelium defect areas in the two treatment groups were smaller than that in the control group on 3, 7, 14 and 21d（P 〈0.05）. The corneal epithelium defect areas of the combined group was smaller than that of the CXL group（P 〈0.05） on 7 and 14 d, but there were no statistical differences on 3, 21 and 28 d. The corneal epithelium defects of the two treatment groups have been healed by day 21. The corneal epithelium defects of the control group were healed on 28 d. The diameters of the corneal collagen fiber bundles（42.960 ±7.383 nm in the CXL group and 37.040±4.160 nm in the combined group） were thicker than that of the control group（24.900±1.868 nm）,but there was no difference between the two treatment groups. Some corneal collagen fiber bundles were distorted and with irregular arrangement, a large number of fibroblasts could be seen among them but no inflammatory cells in both treatment groups. CONCLUSION： CXL combined with liposomal amphotericin B have beneficial effects on fungal corneal ulcers. The combined therapy could alleviate corneal inflammattions, accelerate corneal repair, and shorten the course of disease.展开更多
Objective:The overarching objective of this study was to examine the effectiveness of HIV symptom management guidelines in China in reducing the incidence and severity of symptoms and improving patients'quality of...Objective:The overarching objective of this study was to examine the effectiveness of HIV symptom management guidelines in China in reducing the incidence and severity of symptoms and improving patients'quality of life.Methods:We conducted a controlled,pre-and post-implementation design in the HIV/AIDS inpatient unit in Shanghai.Patients recruited from November 2014 to February 2015 were in the intervention group and those from October 2013 to February 2014 were in the control group.There were 74 patients in each group.Participants in the intervention group received interventions based on the HIV symptom management guidelines.Overall symptom severity,depression,and quality of life were measured in two groups at baseline,week 4,and week 8.Results:Totally 126 patients completed the research,65 in the intervention group and 61 in the control group.The total symptom severity scores showed a statistically significant difference between groups across time(P<0.05).It showed that frequencies of fatigue(36.9%vs.44.3%),fever(6.2%vs.11.5%),loss in weight(9.2%vs.16.4%),mouth ulcers(12.3%vs.16.4%),headaches(9.2%vs.19.7%)and depression(F=1.09,P>0.05)in the intervention group were lower than those in the control group in week 8 without statistical significance.The multilevel growth mixture model indicated a greater increase in the total score of quality of life for the group treated according to the symptom management guidelines(P=0.04).Conclusion:The evidence-based HIV symptom management guidelines can improve a patient's quality of life and relieve negative symptoms.The guidelines can be applied in a similar context to other HIV/AIDS units or clinics.展开更多
A novel on-line system composed of supercritical fluid extraction(SFE), dilution line and reverse phase liquid chromatography/mass spectrometry(RPLC/MS) was constructed for on-line extraction and reverse phase separat...A novel on-line system composed of supercritical fluid extraction(SFE), dilution line and reverse phase liquid chromatography/mass spectrometry(RPLC/MS) was constructed for on-line extraction and reverse phase separation of some fat-soluble components in foods. Three columns including a trap column,concentration column and analytical column were used for trapping the fat-soluble components, on-line enrichment and reverse phase separation, respectively. Capsaicinoids were on-line extracted by a CO_2 supercritical fluid, then concentrated and separated by using the C_(18) columns, finally detected by mass spectrometry(MS). Capsaicin eluted at 10.1 min and limit of detection(LOD, S/N=3) for the standard solution is 0.55pg. The linearity was calculated with a value of coefficient of determination(R^2)≥0.998 in the range of 1.1–8.5 ng. Concentrations of capsaicin in the green, yellow, and red bell peppers were determined to be 60.33 ng/g, 31.79 ng/g, 35.38ng/g, respectively.展开更多
BACKGROUND Hemobilia occurs when there is a fistula between hepatic blood vessels and biliary radicles,and represents only a minority of upper gastrointestinal hemorrhages.Causes of hemobilia are varied,but liver absc...BACKGROUND Hemobilia occurs when there is a fistula between hepatic blood vessels and biliary radicles,and represents only a minority of upper gastrointestinal hemorrhages.Causes of hemobilia are varied,but liver abscess rarely causes hemobilia and only a few cases have been reported.Here,we present a case of atypical hemobilia caused by liver abscess that was successfully managed by endoscopic hepatobiliary intervention through endoscopic retrograde cholangiopancreatography(ERCP).CASE SUMMARY A 54-year-old man presented to our emergency department with a history of right upper quadrant abdominal colic and repeated fever for 6 d.Abdominal sonography and enhanced computed tomography revealed that there was an abscess in the right anterior lobe of the liver.During hospitalization,the patient developed upper gastrointestinal bleeding.Upper gastrointestinal endoscopy revealed a duodenal ulcer bleeding that was treated with three metal clamps.However,the hemodynamics was still unstable.Hence,upper gastrointestinal endoscopy was performed again and fresh blood was seen flowing from the ampulla of Vater.Selective angiography did not show any abnormality.An endoscopic nasobiliary drainage(ENBD)tube was inserted into the right anterior bile duct through ERCP,and subsequently cold saline containing(-)-noradrenaline was infused into the bile duct lumen through the ENBD tube with no episode of further bleeding.CONCLUSION Hemobilia should be considered in the development of liver abscess,and endoscopy is essential for diagnosis and management of some cases.展开更多
Developing high efficient Pd-based electrocatalysts for oxygen reduction reaction(ORR) is still challenging for alkaline membrane fuel cell,since the strong oxygen adsorption energy and easy agglomerative intrinsic pr...Developing high efficient Pd-based electrocatalysts for oxygen reduction reaction(ORR) is still challenging for alkaline membrane fuel cell,since the strong oxygen adsorption energy and easy agglomerative intrinsic properties. In order to simultaneously solve these problems, Pd/Co_(3)O_(4)–N–C multidimensional materials with porous structures is designed as the ORR catalysts. In details, the ZIF-67 with polyhedral structure was firstly synthesized and then annealed at high-temperature to prepare the N-doped Co_(3)O_(4)carbon-based material, which was used to homogeneously confine Pd nanoparticles and obtained the Pd/Co_(3)O_(4)–N–C series catalysts. The formation of Co–N and C–N bond could provide efficient active sites for ORR. Simultaneously, the strong electronic interaction in the interface between the Pd and N-doped Co_(3)O_(4)could disperse and avoid the agglomeration of Pd nanoparticles and ensure the exposure of active sites, which is crucial to lower the energy barrier toward ORR and substantially enhance the ORR kinetics. Hence, the Pd/Co_(3)O_(4)–N–C nanocompounds exhibited excellent ORR catalytic performance, ideal Pd mass activity, and durability in 0.1 mol L-1KOH solution compared with Co_(3)O_(4)–N–C and Pd/C. The scalable synthesis method, relatively low cost, and excellent electrochemical ORR performance indicated that the obtained Pd/Co_(3)O_(4)–N–C electrocatalyst had the potential for application on fuel cells.展开更多
In this work, the transient free surface of con- tainer filling with non-linear constitutive equation＇s fluids is numerically investigated by the smoothed particle hydrody- namics （SPH） method. Specifically, the fi...In this work, the transient free surface of con- tainer filling with non-linear constitutive equation＇s fluids is numerically investigated by the smoothed particle hydrody- namics （SPH） method. Specifically, the filling process of a square container is considered for non-linear polymer fluids based on the Cross model. The validity of the presented SPH is first verified by solving the Newtonian fluid and Oldroyd- B fluid jet. Various phenomena in the filling process are shown, including the jet buckling, jet thinning, splashing or spluttering, steady filling. Moreover, a new phenomenon of vortex whirling is more evidently observed for the Cross model fluid compared with the Newtonian fluid case.展开更多
The precipitation behavior of γ′ phase,under various interrupt cooling tests after 1170℃,solution treatment was examined.The results indicate that the size of secondary γ′ precipitates increases with the decrease...The precipitation behavior of γ′ phase,under various interrupt cooling tests after 1170℃,solution treatment was examined.The results indicate that the size of secondary γ′ precipitates increases with the decrease of interrupt temperature,and the shape changes from spherical to butterfly like.The fine tertiary γ′ can form either during the post cool air quenching at high interrupt-temperatures,or during the specified 5℃ min-1cooling.Air quenching at high temperatures cannot suppress further nucleation of tertiary γ′ phase.展开更多
基金supported by the Science and Technology Innovation Program of Hunan Province(2022RC1148)the Natural Science Foundation of Hunan Province(2022JJ31009,2022JJ50260)+4 种基金the Program for Science and Technology of Changsha,China(kh2301028)the Science and Technology Innovation Plan Project of Hunan Province(2023NK2033)the Innovation Leading Plan Project of Hunan Province(2021GK4022)the“Kemen Food”Graduate Science and Technology Innovation Project of Central South University of Forestry and Technology(2023KMCX02)the Graduate Science and Technology Innovation Fund Project of Hunan Province(QL20220182).
文摘Alzheimer’s disease(AD)is a neurodegenerative disorder associated with brain aging,and the accumulation ofβ-amyloid(Aβ)and hyperphosphorylated Tau proteins are key pathological features.Currently,drugs for the treatment of AD are mainly single-targeted,but the complex pathogenesis of AD makes it difficult to achieve the desired results.Therefore,the development of multitargeted therapies is crucial for future interventions.Rice bran oil(RBO)has been recognized as an edible oil with several health benefits,but its effects on AD caused by brain aging remain underexplored.In this study,the effects of RBO on memory dysfunction in D-galactose(D-gal)mice and its molecular mechanisms were investigated via in vivo and in silico methods from the perspective of AD pathologies.Our results suggested that compounds in RBO could modulate the activities of Aβprecursor protein cleaving enzyme 1(BACE1),mitogen-activated protein kinase 3(MAPK3),matrix metalloproteinase 3(MMP3),and intercellular adhesion molecule 1(ICAM1),leading to inhibition of Aβaccumulation and Tau protein hyperphosphorylation.Moreover,RBO reduced Aβ-induced oxidative stress by inhibiting the activity of mouse double minute 2 homolog(MDM2)and cyclic adenosine monophosphate(cAMP)response element binding protein binding protein(CREBBP),and attenuated neuroinflammation by inhibiting the activity of nitric oxide synthase 2(NOS2)and reducing Aβaccumulation and Tau protein hyperphosphorylation.Additionally,α-linolenic acid in RBO exhibited inhibitory effects on D-gal-induced apoptosis in PC12 cells through modulation of NOS2,MDM2,ICAM1,and phospho-extracellular signal-regulated kinase 1/2(p-ERK1/2).Similarly,stigmastanol inhibited apoptosis in D-gal-induced PC12 cells through the regulation of NOS2.Thus,RBO can be considered as a potential functional food to attenuate AD owing to its multicomponent and multitarget effects.
基金Supported by the Zhejiang Provincial Science and Technology Plan for Traditional Chinese Medicine,No.2025ZL594Municipal-Level Science and Technology Plan Project of Zhejiang Province,No.2023ZD039.
文摘BACKGROUND Diabetic foot ulcers(DFUs)present a significant healthcare challenge attributable to their high rates of disability and the limitations of applied traditional nursing approaches.Effective management is critical for uneventful health outcomes.AIM To investigate the effects of multidisciplinary team(MDT)nursing interventions and blood glucose control on the negative emotions and satisfaction of DFUhealing patients.METHODS This retrospective cohort study included 115 patients with DFUs,divided into MDT and blood glucose control intervention group(n=60)and standard care control group(n=55).The comparison factors were wound area,new granulation tissue coverage area,wound healing rate,2-hour postprandial blood glucose level,fasting plasma glucose level,Hamilton Anxiety Scale score,Hamilton Depression Scale score,and nursing satisfaction.RESULTS After 4 weeks,the average wound area reduced from 22.04±6.48 cm^(2)to 11.96±3.63 cm^(2)(P<0.05).New granulation tissue coverage area reached 52.85±18.39 cm^(2) for the intervention group and 28.39±9.94 cm^(2)(P<0.05)in the control group,respectively.The healing rate was significantly higher in the intervention group than in the control group(91.7%vs 76.4%,P<0.05).Fasting plasma glucose decreased more sharply in the intervention group(from 8.36±0.98 mmol/L to 6.91±1.23 mmol/L)than in the control group(8.41±1.05 mmol/L to 7.81±1.27 mmol/L),with the intervention group maintaining significantly lower levels(P<0.05).The intervention group demonstrated a significantly greater reduction in 2-hour postprandial blood glucose levels(11.35±2.67 mmol/L to 7.52±1.38 mmol/L)compared to the control group(11.61±3.01 mmol/L to 8.72±1.63 mmol/L;P<0.05).Hamilton Anxiety Scale and Hamilton Depression Scale scores were significantly lower in the intervention group(P<0.05).Patient satisfaction with nursing was 93.33%and 74.55%in the intervention and control groups,respectively(P<0.05).CONCLUSION MDT combined with blood glucose control enhanced healing rates and positively influenced emotional well-being and satisfaction among patients.This strategy holds potential for application in clinical practice.
基金National Natural Science Foundation of China(32072780,31872374)National Key Research and Development Program of China(2016YFD0500501,2018YFD0500405)Earmarked Fund for Jiangsu Agricultural Industry Technology System(JATS[2024]).
文摘Background Chronic heat stress(CHS)is a detrimental environmental stressor with a negative impact on the meat quality of broilers.However,the underlying mechanisms are not fully understood.This study investigates the effects of CHS on long non-coding RNA(lncRNA)expression and muscle injury in broilers,with a focus on its implications for meat quality.Results The results showed that CHS diminished breast muscle yield,elevated abdominal fat deposition,induced cellular apoptosis(P<0.05),and caused myofibrosis.Transcriptomic analysis revealed 151 differentially expressed(DE)lncRNAs when comparing the normal control(NC)and HS groups,214 DE lncRNAs when comparing the HS and PF groups,and 79 DE lncRNAs when comparing the NC and pair-fed(PF)groups.After eliminating the confounding effect of feed intake,68 lncRNAs were identified,primarily associated with cellular growth and death,signal transduc-tion,and metabolic regulation.Notably,the apoptosis-related pathway P53,lysosomes,and the fibrosis-related gene TGF-β2 were significantly upregulated by lncRNAs.Conclusions These findings indicate that chronic heat stress induces cellular apoptosis and muscle injury through lncRNA,leading to connective tissue accumulation,which likely contributes to reduced breast muscle yield and meat quality in broilers.
基金supported by the China National Natural Science Foundation(Grant No.32102417)Henan Key R&D Project,China(Grant No.221111110700)+1 种基金Henan Academy of Agricultural Sciences“Innovation and Utilization of Characteristic Flowers and Trees Germplasm”Team Project(Grant Nos.2023TD35 and2024TD26)Hunan Provincial Natural Science Foundation of China(Grant No.2024JJ6711),Natural Science Foundation of Guangxi(Grant No.2024JJB130300)。
文摘Wintersweet(Chimonanthus praecox),a well-known fragrant flowering shrub,is extensively planted for ornamental purpose and production of floral essential oil.Although the tepal color of wintersweet varieties exhibits the most remarkable diversity,variations in the floral scent traits are also noticeable across different cultivars.In this study,the floral volatile organic compounds(VOCs)in three wintersweet cultivars,‘Yanlingsuxin’,‘Yuxiang’,and‘Hongyun’were detected via GC-MS coupled with OAV.The distinct floral aromas of the three cultivars were primarily attributed to benzyl alcohol(abundant in‘Yuxiang’),linalool(abundant in‘Yanlingsuxin’),(-)-γ-cadinene and eugenol(abundant in‘Hongyun’).Integrated analyses of metabolome and transcriptome showed that an R2R3-MYB transcription factor gene,CpODO1,potentially have a crucial regulatory function in controlling the production of multiple aroma compounds.Overexpression of CpODO1 can enhance the production of benzyl alcohol in transgenic tobacco flowers.Analysis of DAP-seq data,EMSA and dual-luciferase assay revealed that CpODO1 predominantly regulate the expression of CpCYP71,a cytochrome P450 gene encoding a key enzyme in the production of benzyl alcohol,and the transcriptional regulation of CpODO1 is driven by CpEOBII.The identification of polymorphisms in the MYB binding cis-motifs of CpCYP71 and CpODO1 promoters revealed the regulatory mechanism underlying the varied synthesis of benzyl alcohol in three wintersweet cultivars.This study provides new anchor points for floral scent quality improvement breeding of wintersweet,and the profusion of wintersweet germplasm can serve as a material basis for developing various aroma products.
文摘The Hydro-Viscous Drive(HVD)speed regulating system finds extensive application in air transport transmission systems to regulate the stepless speed or conduct overload protection.However,its intrinsic hysteretic behaviors,such as the asymmetric hysteretic and dead zone,could introduce inaccuracy and delay in control applications,posing challenges to system regulation.This paper investigates a Nonlinear Hysteresis Compensation Control(NHCC)that consists of two parts to control the HVD output speed by operating the valve under different engine operating conditions.In the first part,the Inverse Hysteresis Compensator(IHC)based on major loop data is introduced for the asymmetric hysteresis characterization and compensation of the HVD speed control system of the power generation and distribution,which aims to reduce the hysteresis and dead zone effect and expand the effective input range.In the second part,the Active Disturbance Rejection Controller(ADRC)is employed to mitigate the hysteresis effects of the compensated system and remove the steady-state error,which allows real-time compensation of the estimated perturbations as state feedback to achieve the required performance.An experimental laboratory station has been fabricated to evaluate the proposed method.The test results show that the NHCC method can regulate the fan speed to the desired value(45 r/min at steady state)and broaden the effective input range to the full range under different engine conditions.Besides,the proposed control method can reduce the non-linearity of the input and output curves(from 18%to 4%)and compensate for the asymmetric hysteresis(from 38%to 5%).
基金supported by the Fundamental Research Funds for the Central Universities,China(Grant Nos.:2023CDJXY-009 and 2023CDJYGRH-YB07)the National Natural Science Foundation of China(Grant No.:82172888)the Natural Science Foundation Project of Chongqing Science and Technology Commission(Grant No.:cstc2020jcyj-msxmX0154).
文摘Gastrointestinal(GI)cancers are prevalent globally,with leading incidence and mortality rates among malignant tumors.Despite notable advancements in surgical resection,radiotherapy,and chemotherapy,the overall survival rates remain low.Hence,it is imperative to explore alternative approaches that enhance patient outcomes.Cluster of differentiation 47(CD47),serving as an early diagnostic marker,is predominantly overexpressed in GI cancers and associated with poor prognosis.Targeting the CD47-signal regulatory protein alpha(SIRPa)signaling pathway may provide a novel strategy for GI cancers treatment.This study summarizes current knowledge of the structure and function of CD47 and SIRPa,their roles in signaling pathways,the prognostic significance of CD47,therapeutic strategies targeting the CD47-SIRPα signaling pathway in GI cancer,and highlights key issues for future investigations.
文摘AIM: To determine the possible predisposing factors of bezoar-induced small bowel obstruction(b I-Sb O) and to discuss the diagnostic value of multi-slice spiral computed tomography,particularly contrast-enhanced scanning,in this condition.METHODS: A total of 35 b I-Sb O cases treated at our hospital from January 2007 to December 2013 were retrospectively analysed.Complete clinical and computed tomography(CT) data of the patients were available and confirmed by surgery.SbO was clinically diagnosed on the basis of clinical manifestations.Of the 35 patients,18 underwent abdominal and pelvic CT planar scanning with GE 64-slice spiral CT and 17 underwent abdominal and pelvic CT planar scanning with GE 64-slice spiral CT combined with contrastenhanced examination.Original images were processed using a GE ADW4.3 workstation to obtain MPR,CPR,MIP and CTA images.The images of all patients were evaluated by two abdominal imaging experts.The main analytical contents of planar scanning included intestinal bezoar conditions,changes in the intestinal wall and changes in peri-intestinal conditions.Vascular hyperaemia and arterial blood supply conditions at a specific obstruction site and the distal end of the obstruction site were evaluated through contrastenhanced examination.RESULTS: The proportion of males to females among the 35 cases was 1:1.69(13:22); median age was 63.3 years.The following cases were observed: 29(82.8%) cases occurred in autumn and winter and showed a history of consuming high amounts of persimmon and hawthorn; 19(54.3%) cases revealed a history of gastrointestinal surgery; 19 exhibited incomplete dentition,with missing partial or whole posterior teeth; 26 suffered from obstruction at the ileum.A total of 51 bezoars were found in these patients,of whom 16(45.7%) had multiple bezoars.CT planar scanning of bezoars showed lumps with mottled gas inside the intestinal cavity.Furthermore,9 cases of bezoars had envelopes and 11 cases were accompanied with thickening of the distal wall of the obstructed bowel.Scanning of 17 cases was enhanced; the results revealed that the mesenteric blood vessels at the obstruction site and the proximal site were dilated,and a total of 7 cases were accompanied with distal vascular dilation and intestinal wall thickening.CONCLUSION: b I-Sb O exhibits regional and seasonal characteristics.CT planar and contrast-enhanced scanning can be applied to diagnose and observe vascular conditions in obstructed zones.
基金financially supported by the National Natural Science Foundation of China(NSFC)(52274295)the Natural Science Foundation of Hebei Province(E2020501001,E2021501029,A2021501007,E2022501028,E2022501029)+5 种基金the Natural Science Foundation-Steel,the Iron Foundation of Hebei Province(No.E2022501030)the Performance subsidy fund for Key Laboratory of Dielectric and Electrolyte Functional Material Hebei Province(22567627H)the Science and Technology Project of Hebei Education Department(ZD2022158)the Central Guided Local Science and Technology Development Fund Project of Hebei province(226Z4401G)the China Scholarship Council(No.202206080061,202206050119)the 2023 Hebei Provincial Postgraduate Student Innovation Ability training funding project(CXZZSS2023195)。
文摘The recycling and reutilization of spent lithium-ion batteries(LIBs)have become an important measure to alleviate problems like resource scarcity and environmental pollution.Although some progress has been made,battery recycling technology still faces challenges in terms of efficiency,effectiveness and environmental sustainability.This review aims to systematically review and analyze the current status of spent LIB recycling,and conduct a detailed comparison and evaluation of different recycling processes.In addition,this review introduces emerging recycling techniques,including deep eutectic solvents,molten salt roasting,and direct regeneration,with the intent of enhancing recycling efficiency and diminishing environmental repercussions.Furthermore,to increase the added value of recycled materials,this review proposes the concept of upgrading recycled materials into high value-added functional materials,such as catalysts,adsorbents,and graphene.Through life cycle assessment,the paper also explores the economic and environmental impacts of current battery recycling and highlights the importance that future recycling technologies should achieve a balance between recycling efficiency,economics and environmental benefits.Finally,this review outlines the opportunities and challenges of recycling key materials for next-generation batteries,and proposes relevant policy recommendations to promote the green and sustainable development of batteries,circular economy,and ecological civilization.
基金This work was supported by the National Natural Science Foundation of China(Nos.81772688 and 81372698)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)+1 种基金the Research Foundation for Talented Scholars of Xuzhou Medical University(No.RC20552223)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX20_2463),China。
文摘Hypoxia,as an important hallmark of the tumor microenvironment,is a major cause of oxidative stress and plays a central role in various malignant tumors,including glioblastoma.Elevated reactive oxygen species(ROS)in a hypoxic microenvironment promote glioblastoma progression;however,the underlying mechanism has not been clarified.Herein,we found that hypoxia promoted ROS production,and the proliferation,migration,and invasion of glioblastoma cells,while this promotion was restrained by ROS scavengers N-acetyl-L-cysteine(NAC)and diphenyleneiodonium chloride(DPI).Hypoxia-induced ROS activated hypoxia-inducible factor-1α(HIF-1α)signaling,which enhanced cell migration and invasion by epithelial-mesenchymal transition(EMT).Furthermore,the induction of serine protease inhibitor family E member 1(SERPINE1)was ROS-dependent under hypoxia,and HIF-1αmediated SERPINE1 increase induced by ROS via binding to the SERPINE1 promoter region,thereby facilitating glioblastoma migration and invasion.Taken together,our data revealed that hypoxia-induced ROS reinforce the hypoxic adaptation of glioblastoma by driving the HIF-1α-SERPINE1 signaling pathway,and that targeting ROS may be a promising therapeutic strategy for glioblastoma.
文摘Semen from 5210 sperm bank donors was analyzed and trends in semen quality were evaluated at Shandong Human Sperm Bank between 2008 and 2014. After 2-7 days of abstinence, semen samples were collected. Measurements of semen volume, sperm concentration, sperm forward motility, and total sperm count were performed. There were significant declining trends in semen volume, sperm concentration, sperm forward motility, and total sperm count. Our results indicate that the quality of semen in this cohort of sperm donors had decreased during the study period.
基金support from the National Natural Science Foundation of China(Grant No.71573085,71974150).
文摘Purpose:Interdisciplinarity is a hot topic in science and technology policy.However,the concept of interdisciplinarity is both abstract and complex,and therefore difficult to measure using a single indicator.A variety of metrics for measuring the diversity and interdisciplinarity of articles,journals,and fields have been proposed in the literature.In this article,we ask whether institutions can be ranked in terms of their(inter-)disciplinary diversity.Design/methodology/approach:We developed a software application(interd_vb.exe)that outputs the values of relevant diversity indicators for any document set or network structure.The software is made available,free to the public,online.The indicators it considers include the advanced diversity indicators Rao-Stirling(RS)diversity and DIV*,as well as standard measures of diversity,such as the Gini coefficient,Shannon entropy,and the Simpson Index.As an empirical demonstration of how the application works,we compared the research portfolios of 42“Double First-Class”Chinese universities across Web of Science Subject Categories(WCs).Findings:The empirical results suggest that DIV*provides results that are more in line with one’s intuitive impressions than RS,particularly when the results are based on sampledependent disparity measures.Furthermore,the scores for diversity are more consistent when based on a global disparity matrix than on a local map.Research limitations:“Interdisciplinarity”can be operationalized as bibliographic coupling among(sets of)documents with references to disciplines.At the institutional level,however,diversity may also indicate comprehensiveness.Unlike impact(e.g.citation),diversity and interdisciplinarity are context-specific and therefore provide a second dimension to the evaluation.Policy or practical implications:Operationalization and quantification make it necessary for analysts to make their choices and options clear.Although the equations used to calculate diversity are often mathematically transparent,the specification in terms of computer code helps the analyst to further precision in decisions.Although diversity is not necessarily a goal of universities,a high diversity score may inform potential policies concerning interdisciplinarity at the university level.Originality/value:This article introduces a non-commercial online application to the public domain that allows researchers and policy analysts to measure“diversity”and“interdisciplinarity”using the various indicators as encompassing as possible for any document set or network structure(e.g.a network of co-authors).Insofar as we know,such a professional computing tool for evaluating data sets using diversity indicators has not yet been made available online.
基金supported by the National Natural Science Foundation of China(31640001 and T2350005 to C.X.,U21A20148 to X.Z.and C.X.)Ministry of Science and Technology of China(2021ZD0140300 to C.X.)Presidential Foundation of Hefei Institutes of Physical Science,Chinese Academy of Sciences(Y96XC11131,E26CCG27,and E26CCD15 to C.X.,E36CWGBR24B and E36CZG14132 to T.C.)。
文摘Birds exhibit extraordinary mobility and remarkable navigational skills,obtaining guidance cues from the Earth’s magnetic field for orientation and long-distance movement.Bird species also show tremendous diversity in navigation strategies,with considerable differences even within the same taxa and among individuals from the same population.The highly conserved iron and iron-sulfur cluster binding magnetoreceptor(MagR)protein is suggested to enable animals,including birds,to detect the geomagnetic field and navigate accordingly.Notably,MagR is also implicated in other functions,such as electron transfer and biogenesis of iron-sulfur clusters,raising the question of whether variability exists in its biochemical and biophysical features among species,particularly birds.In the current study,we conducted a comparative analysis of MagR from two different bird species,including the migratory European robin(Erithacus rubecula)and the homing pigeon(Columba livia).Sequence alignment revealed an extremely high degree of similarity between the MagRs of these species,with only three sequence variations.Nevertheless,two of these variations underpinned significant differences in metal binding capacity,oligomeric state,and magnetic properties.These findings offer compelling evidence for the marked differences in MagR between the two avian species,potentially explaining how a highly conserved protein can mediate such diverse functions.
基金This work was funded by the National Natural Science Foundation of China,No.81971006(to DSG)the Postgraduate Research and Practice Innovation Program of Jiangsu Province of China,Nos.KYCX18_2193(to MYS),KYCX18_2171(to CXT).
文摘Glial cell line-derived neurotrophic factor(GDNF)plays an important role in the protection of dopaminergic neurons,but there are few reports of the relationship between GDNF and its precursors(α-pro-GDNF andβ-pro-GDNF)and cognitive impairment in Parkinson’s disease.This study aimed to investigate the relationship between the serum levels of GDNF and its precursors and cognitive impairment in Parkinson’s disease,and to assess their potential as a diagnostic marker.Fifty-three primary outpatients and hospitalized patients with Parkinson’s disease(23 men and 30 women)with an average age of 66.58 years were enrolled from the Affiliated Hospital of Xuzhou Medical University of China in this case-control study.The patients were divided into the Parkinson’s disease with cognitive impairment group(n=27)and the Parkinson’s disease with normal cognitive function group(n=26)based on their Mini-Mental State Examination,Montreal Cognitive Assessment,and Clinical Dementia Rating scores.In addition,26 age-and sex-matched healthy subjects were included as the healthy control group.Results demonstrated that serum GDNF levels were significantly higher in the Parkinson’s disease with normal cognitive function group than in the other two groups.There were no significant differences in GDNF precursor levels among the three groups.Correlation analysis revealed that serum GDNF levels,GDNF/α-pro-GDNF ratios,and GDNF/β-pro-GDNF ratios were moderately or highly correlated with the Mini-Mental State Examination,Montreal Cognitive Assessment,and Clinical Dementia Rating scores.To explore the risk factors for cognitive impairment in patients with Parkinson’s disease,logistic regression analysis and stepwise linear regression analysis were performed.Both GDNF levels and Hoehn-Yahr stage were risk factors for cognitive impairment in Parkinson’s disease,and were the common influencing factors for cognitive scale scores.Neitherα-pro-GDNF norβ-pro-GDNF was risk factors for cognitive impairment in Parkinson’s disease.A receiver operating characteristic curve of GDNF was generated to predict cognitive function in Parkinson’s disease(area under the curve=0.859).This result indicates that the possibility that serum GDNF can correctly distinguish whether patients with Parkinson’s disease have cognitive impairment is 0.859.Together,these results suggest that serum GDNF may be an effective diagnostic marker for cognitive impairment in Parkinson’s disease.However,α-pro-GDNF andβ-pro-GDNF are not useful for predicting cognitive impairment in this disease.This study was approved by Ethics Committee of the Affiliated Hospital of Xuzhou Medical University,China(approval No.XYFY2017-KL047-01)on November 30,2017.
基金Supported by Nature Science Fundamental Research Planned Projects of Shaanxi Province(No.2011JE005No.2012JM4023)Science and Technology Planned Projects of Xi'an[No.SF1207(1)]
文摘AIM： To observe the therapeutic effect of corneal collagen cross-linking（CXL） in combination with liposomal amphotericin B in fungal corneal ulcers.METHODS： New Zealand rabbits were induced fungal corneal ulcers by scratching and randomly divided into 3groups, i.e. control, treated with CXL, and combined therapy of CXL with 0.25% liposomal amphotericin B（n =5 each）. The corneal lesions were documented with slit-lamp and confocal microscopy on 3, 7, 14, 21 and 28 d after treatment. The corneas were examined with transmission electron microscopy（TEM） at 4wk.RESULTS： A rabbit corneal ulcer model of Fusarium was successfully established. The corneal epithelium defect areas in the two treatment groups were smaller than that in the control group on 3, 7, 14 and 21d（P 〈0.05）. The corneal epithelium defect areas of the combined group was smaller than that of the CXL group（P 〈0.05） on 7 and 14 d, but there were no statistical differences on 3, 21 and 28 d. The corneal epithelium defects of the two treatment groups have been healed by day 21. The corneal epithelium defects of the control group were healed on 28 d. The diameters of the corneal collagen fiber bundles（42.960 ±7.383 nm in the CXL group and 37.040±4.160 nm in the combined group） were thicker than that of the control group（24.900±1.868 nm）,but there was no difference between the two treatment groups. Some corneal collagen fiber bundles were distorted and with irregular arrangement, a large number of fibroblasts could be seen among them but no inflammatory cells in both treatment groups. CONCLUSION： CXL combined with liposomal amphotericin B have beneficial effects on fungal corneal ulcers. The combined therapy could alleviate corneal inflammattions, accelerate corneal repair, and shorten the course of disease.
基金This project was funded by National Natural Science Foundation of China(Grant No.71673057)
文摘Objective:The overarching objective of this study was to examine the effectiveness of HIV symptom management guidelines in China in reducing the incidence and severity of symptoms and improving patients'quality of life.Methods:We conducted a controlled,pre-and post-implementation design in the HIV/AIDS inpatient unit in Shanghai.Patients recruited from November 2014 to February 2015 were in the intervention group and those from October 2013 to February 2014 were in the control group.There were 74 patients in each group.Participants in the intervention group received interventions based on the HIV symptom management guidelines.Overall symptom severity,depression,and quality of life were measured in two groups at baseline,week 4,and week 8.Results:Totally 126 patients completed the research,65 in the intervention group and 61 in the control group.The total symptom severity scores showed a statistically significant difference between groups across time(P<0.05).It showed that frequencies of fatigue(36.9%vs.44.3%),fever(6.2%vs.11.5%),loss in weight(9.2%vs.16.4%),mouth ulcers(12.3%vs.16.4%),headaches(9.2%vs.19.7%)and depression(F=1.09,P>0.05)in the intervention group were lower than those in the control group in week 8 without statistical significance.The multilevel growth mixture model indicated a greater increase in the total score of quality of life for the group treated according to the symptom management guidelines(P=0.04).Conclusion:The evidence-based HIV symptom management guidelines can improve a patient's quality of life and relieve negative symptoms.The guidelines can be applied in a similar context to other HIV/AIDS units or clinics.
基金supported by the National Natural Science Foundation of China (No. 21621003)
文摘A novel on-line system composed of supercritical fluid extraction(SFE), dilution line and reverse phase liquid chromatography/mass spectrometry(RPLC/MS) was constructed for on-line extraction and reverse phase separation of some fat-soluble components in foods. Three columns including a trap column,concentration column and analytical column were used for trapping the fat-soluble components, on-line enrichment and reverse phase separation, respectively. Capsaicinoids were on-line extracted by a CO_2 supercritical fluid, then concentrated and separated by using the C_(18) columns, finally detected by mass spectrometry(MS). Capsaicin eluted at 10.1 min and limit of detection(LOD, S/N=3) for the standard solution is 0.55pg. The linearity was calculated with a value of coefficient of determination(R^2)≥0.998 in the range of 1.1–8.5 ng. Concentrations of capsaicin in the green, yellow, and red bell peppers were determined to be 60.33 ng/g, 31.79 ng/g, 35.38ng/g, respectively.
基金Supported by the National Clinical Key Subject of China,No. 41732113
文摘BACKGROUND Hemobilia occurs when there is a fistula between hepatic blood vessels and biliary radicles,and represents only a minority of upper gastrointestinal hemorrhages.Causes of hemobilia are varied,but liver abscess rarely causes hemobilia and only a few cases have been reported.Here,we present a case of atypical hemobilia caused by liver abscess that was successfully managed by endoscopic hepatobiliary intervention through endoscopic retrograde cholangiopancreatography(ERCP).CASE SUMMARY A 54-year-old man presented to our emergency department with a history of right upper quadrant abdominal colic and repeated fever for 6 d.Abdominal sonography and enhanced computed tomography revealed that there was an abscess in the right anterior lobe of the liver.During hospitalization,the patient developed upper gastrointestinal bleeding.Upper gastrointestinal endoscopy revealed a duodenal ulcer bleeding that was treated with three metal clamps.However,the hemodynamics was still unstable.Hence,upper gastrointestinal endoscopy was performed again and fresh blood was seen flowing from the ampulla of Vater.Selective angiography did not show any abnormality.An endoscopic nasobiliary drainage(ENBD)tube was inserted into the right anterior bile duct through ERCP,and subsequently cold saline containing(-)-noradrenaline was infused into the bile duct lumen through the ENBD tube with no episode of further bleeding.CONCLUSION Hemobilia should be considered in the development of liver abscess,and endoscopy is essential for diagnosis and management of some cases.
基金funded by National Natural Science Foundation of China (21975129)Natural Science Foundation of Jiangsu Province (BK20190759)+1 种基金Nanjing Forestry UniversityPostgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX21_0337)。
文摘Developing high efficient Pd-based electrocatalysts for oxygen reduction reaction(ORR) is still challenging for alkaline membrane fuel cell,since the strong oxygen adsorption energy and easy agglomerative intrinsic properties. In order to simultaneously solve these problems, Pd/Co_(3)O_(4)–N–C multidimensional materials with porous structures is designed as the ORR catalysts. In details, the ZIF-67 with polyhedral structure was firstly synthesized and then annealed at high-temperature to prepare the N-doped Co_(3)O_(4)carbon-based material, which was used to homogeneously confine Pd nanoparticles and obtained the Pd/Co_(3)O_(4)–N–C series catalysts. The formation of Co–N and C–N bond could provide efficient active sites for ORR. Simultaneously, the strong electronic interaction in the interface between the Pd and N-doped Co_(3)O_(4)could disperse and avoid the agglomeration of Pd nanoparticles and ensure the exposure of active sites, which is crucial to lower the energy barrier toward ORR and substantially enhance the ORR kinetics. Hence, the Pd/Co_(3)O_(4)–N–C nanocompounds exhibited excellent ORR catalytic performance, ideal Pd mass activity, and durability in 0.1 mol L-1KOH solution compared with Co_(3)O_(4)–N–C and Pd/C. The scalable synthesis method, relatively low cost, and excellent electrochemical ORR performance indicated that the obtained Pd/Co_(3)O_(4)–N–C electrocatalyst had the potential for application on fuel cells.
基金support by the National Basic Research Program of China (973) (2012CB025903)the National Natural Science Foundation of China (10871159)Basic Research Program of China(2005CB321704)
文摘In this work, the transient free surface of con- tainer filling with non-linear constitutive equation＇s fluids is numerically investigated by the smoothed particle hydrody- namics （SPH） method. Specifically, the filling process of a square container is considered for non-linear polymer fluids based on the Cross model. The validity of the presented SPH is first verified by solving the Newtonian fluid and Oldroyd- B fluid jet. Various phenomena in the filling process are shown, including the jet buckling, jet thinning, splashing or spluttering, steady filling. Moreover, a new phenomenon of vortex whirling is more evidently observed for the Cross model fluid compared with the Newtonian fluid case.
基金supported by the Science and Technology Project of Beijing (No. D09080300510901)National High Technology Research and Development Pro-gram of China (No. 2012AA03A514)
文摘The precipitation behavior of γ′ phase,under various interrupt cooling tests after 1170℃,solution treatment was examined.The results indicate that the size of secondary γ′ precipitates increases with the decrease of interrupt temperature,and the shape changes from spherical to butterfly like.The fine tertiary γ′ can form either during the post cool air quenching at high interrupt-temperatures,or during the specified 5℃ min-1cooling.Air quenching at high temperatures cannot suppress further nucleation of tertiary γ′ phase.
