HSCs were divided into four groups,namely control group(A),MyD88 siRNA group(B),curcumin group(C),and curcumin+MyD88 siRNA group(D).Groups B and D were subjected to transient transfection with siRNA for 48h.Groups C a...HSCs were divided into four groups,namely control group(A),MyD88 siRNA group(B),curcumin group(C),and curcumin+MyD88 siRNA group(D).Groups B and D were subjected to transient transfection with siRNA for 48h.Groups C and D were incubated with curcumin(25μmol/L)for 24h.MyD88 protein expression was observed by Western Blot,apoptosis was detected by flow cytometry,展开更多
文摘HSCs were divided into four groups,namely control group(A),MyD88 siRNA group(B),curcumin group(C),and curcumin+MyD88 siRNA group(D).Groups B and D were subjected to transient transfection with siRNA for 48h.Groups C and D were incubated with curcumin(25μmol/L)for 24h.MyD88 protein expression was observed by Western Blot,apoptosis was detected by flow cytometry,