SWEET(sugars will eventually be exported transporter)基因在植物开花过程中具有重要的作用,但AcSWEET11在菠萝成花中的作用机制尚不清楚。通过鉴定成花过程中与AcSWEET11的互作蛋白,为菠萝成花机制的解析奠定基础。本研究利用共转...SWEET(sugars will eventually be exported transporter)基因在植物开花过程中具有重要的作用,但AcSWEET11在菠萝成花中的作用机制尚不清楚。通过鉴定成花过程中与AcSWEET11的互作蛋白,为菠萝成花机制的解析奠定基础。本研究利用共转化的方法在菠萝成花过程的cDNA膜文库中筛选AcSWEET11的互作蛋白,分析候选蛋白的表达量。结果表明,pBT3-STE-AcSWEET11+pPR3-N对NMY51酵母细胞无毒性,但有自激活活性。进一步研究结果显示,在TDO/3?AT培养基和QDO培养基上自激活受到抑制。利用该系统筛选到了81个阳性克隆,经测序鉴定出48个与AcSWEET11互作的候选蛋白,包括E3 ubiquitin-protein ligase RING1-like、Trehalose-phosphate synthase 7、Cytochrome P450、TranscriptionfactorLUX等。GO和KEGG分析结果显示,48个蛋白主要分布在细胞进程、代谢过程、刺激反应和催化活性等生物过程,参与脂类代谢、氨基酸代谢和碳水化合物代谢、信号转导和运输与分解代谢等新陈代谢途径。Trehalose-phosphate synthase 7(XP_020105459.1)、Protein TIFY 3-like(XP_020082835.1)、40S ribosomal protein S27(XP_020092770.1)、Heterogeneous nuclear ribonucleoprotein 1-like(XP_020112516.1)等4个基因与AcSWEET11表达趋势一致,在菠萝成花过程中下调表达;Dihydrolipoyl dehydrogenase 2(XP_020113798.1)、Putative lipid-transfer protein DIR1(XP_020086640.1)、clathrin assembly protein At4g32285(XP_020108161.1)等3个基因在菠萝成花过程中上调表达。这些结果表明,AcSWEET11可能通过与Trehalose-phosphatesynthase7等蛋白发生互作,参与菠萝成花过程。本研究进一步丰富了AcSWEET11的蛋白互作网络,为AcSWEET11在菠萝成花中的调控机制的解析奠定基础。展开更多
Hydroisomerization of n-heptane is an efficient method for producing gasoline with a high octane number.The focus of this study was to find a highly efficient catalyst that could both promote the conversion of n-hepta...Hydroisomerization of n-heptane is an efficient method for producing gasoline with a high octane number.The focus of this study was to find a highly efficient catalyst that could both promote the conversion of n-heptane and inhibit the cracking side reaction.MIL-101(Cr)is a chromium-based metal-organic framework(MOF)with good hydrothermal stability,and exhibits a three-dimensional pore structure that is similar to that of zeolites.Using phosphomolybdic acid(PMA;H3PMo12O40·xH2O)can increase the number of Brønsted acid sites on MIL-101(Cr),which contributes to improving the catalytic performance during isomerization.In this study,0.4%Pt/PMA-MIL-101(Cr)catalyst was successfully crystallized at 220℃using a hydrothermal synthetic method.The results showed that the synthesized samples were mesoporousmicroporous composite materials with the typical octahedral structure,and the MIL-101(Cr)framework was not damaged following modification with PMA.It was found that 0.4%Pt30%PMA-MIL-101(Cr)exhibited the best performance for isomerization of n-heptane,with a conversion rate and selectivity at 260°C of 47.6%and 96.6%,respectively.After five hours of reaction,the conversion rate and selectivity of the catalyst remained above 38%and 80%,respectively.展开更多
菠萝栽培种(Ananas comosus var. comosus)的营养生长期较长,制约了其新品种选育效率。收集和筛选营养生长期较短的种质,可为短营养生长期菠萝新品种的选育创制中间材料。从境外收集了23份易成花的野生种质材料,自2008年起进行栽培、繁...菠萝栽培种(Ananas comosus var. comosus)的营养生长期较长,制约了其新品种选育效率。收集和筛选营养生长期较短的种质,可为短营养生长期菠萝新品种的选育创制中间材料。从境外收集了23份易成花的野生种质材料,自2008年起进行栽培、繁殖和性状鉴定。通过对营养生长期的比较和观察,从矮凤梨(A. comosus var. nanus)的体胚苗中发现了1份营养生长期显著缩短的突变材料‘14-1’,其营养生长期约6个月(原品种约12个月),1年开花2次,具有极易成花等特点。‘14-1’无性繁殖后代遗传性状稳定,可作为短营养生长期菠萝育种的亲本资源。展开更多
文摘SWEET(sugars will eventually be exported transporter)基因在植物开花过程中具有重要的作用,但AcSWEET11在菠萝成花中的作用机制尚不清楚。通过鉴定成花过程中与AcSWEET11的互作蛋白,为菠萝成花机制的解析奠定基础。本研究利用共转化的方法在菠萝成花过程的cDNA膜文库中筛选AcSWEET11的互作蛋白,分析候选蛋白的表达量。结果表明,pBT3-STE-AcSWEET11+pPR3-N对NMY51酵母细胞无毒性,但有自激活活性。进一步研究结果显示,在TDO/3?AT培养基和QDO培养基上自激活受到抑制。利用该系统筛选到了81个阳性克隆,经测序鉴定出48个与AcSWEET11互作的候选蛋白,包括E3 ubiquitin-protein ligase RING1-like、Trehalose-phosphate synthase 7、Cytochrome P450、TranscriptionfactorLUX等。GO和KEGG分析结果显示,48个蛋白主要分布在细胞进程、代谢过程、刺激反应和催化活性等生物过程,参与脂类代谢、氨基酸代谢和碳水化合物代谢、信号转导和运输与分解代谢等新陈代谢途径。Trehalose-phosphate synthase 7(XP_020105459.1)、Protein TIFY 3-like(XP_020082835.1)、40S ribosomal protein S27(XP_020092770.1)、Heterogeneous nuclear ribonucleoprotein 1-like(XP_020112516.1)等4个基因与AcSWEET11表达趋势一致,在菠萝成花过程中下调表达;Dihydrolipoyl dehydrogenase 2(XP_020113798.1)、Putative lipid-transfer protein DIR1(XP_020086640.1)、clathrin assembly protein At4g32285(XP_020108161.1)等3个基因在菠萝成花过程中上调表达。这些结果表明,AcSWEET11可能通过与Trehalose-phosphatesynthase7等蛋白发生互作,参与菠萝成花过程。本研究进一步丰富了AcSWEET11的蛋白互作网络,为AcSWEET11在菠萝成花中的调控机制的解析奠定基础。
基金National Natural Science Foundation of China(Grant No.22272129).
文摘Hydroisomerization of n-heptane is an efficient method for producing gasoline with a high octane number.The focus of this study was to find a highly efficient catalyst that could both promote the conversion of n-heptane and inhibit the cracking side reaction.MIL-101(Cr)is a chromium-based metal-organic framework(MOF)with good hydrothermal stability,and exhibits a three-dimensional pore structure that is similar to that of zeolites.Using phosphomolybdic acid(PMA;H3PMo12O40·xH2O)can increase the number of Brønsted acid sites on MIL-101(Cr),which contributes to improving the catalytic performance during isomerization.In this study,0.4%Pt/PMA-MIL-101(Cr)catalyst was successfully crystallized at 220℃using a hydrothermal synthetic method.The results showed that the synthesized samples were mesoporousmicroporous composite materials with the typical octahedral structure,and the MIL-101(Cr)framework was not damaged following modification with PMA.It was found that 0.4%Pt30%PMA-MIL-101(Cr)exhibited the best performance for isomerization of n-heptane,with a conversion rate and selectivity at 260°C of 47.6%and 96.6%,respectively.After five hours of reaction,the conversion rate and selectivity of the catalyst remained above 38%and 80%,respectively.
文摘菠萝栽培种(Ananas comosus var. comosus)的营养生长期较长,制约了其新品种选育效率。收集和筛选营养生长期较短的种质,可为短营养生长期菠萝新品种的选育创制中间材料。从境外收集了23份易成花的野生种质材料,自2008年起进行栽培、繁殖和性状鉴定。通过对营养生长期的比较和观察,从矮凤梨(A. comosus var. nanus)的体胚苗中发现了1份营养生长期显著缩短的突变材料‘14-1’,其营养生长期约6个月(原品种约12个月),1年开花2次,具有极易成花等特点。‘14-1’无性繁殖后代遗传性状稳定,可作为短营养生长期菠萝育种的亲本资源。
