This study examined the role of moral disengagement dual system of self-control in the relationship between parental psychological control and cyberbullying.Participants were involved 802 vocational college students(4...This study examined the role of moral disengagement dual system of self-control in the relationship between parental psychological control and cyberbullying.Participants were involved 802 vocational college students(46.01%females;M=18.11,SD=1.23).They completed measures on parental psychological control,moral disengagement,dual system of self-control(impulse and control system),and cyberbullying.The results from mediation-moderation analysis indicated that parental psychological control directly predicts higher cyberbullying.Specifically,moral disengagement partially mediated this relationship,as higher parental psychological control increases moral disengagement,which in turn elevates the risk of cyberbullying.Furthermore,parental psychological control moderated the relationship between parental control and cyberbullying through impulse control systems within the dual system of self-control.Individuals with high impulsivity scores are more likely to engage in cyberbullying when exposed to high levels of parental psychological control,whereas individuals with low impulsivity scores exhibit a lower incidence of cyberbullying.展开更多
Epilepsy is a brain condition characterized by the recurrence of unprovoked seizures.Recent studies have shown that complement component 3(C3)aggravate the neuronal injury in epilepsy.And our previous studies revealed...Epilepsy is a brain condition characterized by the recurrence of unprovoked seizures.Recent studies have shown that complement component 3(C3)aggravate the neuronal injury in epilepsy.And our previous studies revealed that TRPV1(transient receptor potential vanilloid type 1)is involved in epilepsy.Whether complement C3 regulation of neuronal injury is related to the activation of TRPV1 during epilepsy is not fully understood.We found that in a mouse model of status epilepticus(SE),complement C3 derived from astrocytes was increased and aggravated neuronal injury,and that TRPV 1-knockout rescued neurons from the injury induced by complement C3.Circular RNAs are abundant in the brain,and the reduction of circRad52 caused by complement C3 promoted the expression of TRPV 1 and exacerbated neuronal injury.Mechanistically,disorders of neuron-glia interaction mediated by the C3-TRPV1 signaling pathway may be important for the induction of neuronal injury.This study provides support for the hypothesis that the C3-TRFV1 pathway is involved in the prevention and treatment of neuronal injury and cognitive disorders.展开更多
Objective: The aim of this study was to investigate the prevalence of sarcopenia(SP) and its relationship with gut microbiota alterations in patients with hematological diseases before and after hematopoietic stem cel...Objective: The aim of this study was to investigate the prevalence of sarcopenia(SP) and its relationship with gut microbiota alterations in patients with hematological diseases before and after hematopoietic stem cell transplantation(HSCT).Methods: A total of 108 patients with various hematological disorders were selected from Peking University People’s Hospital. SP was screened and diagnosed based on the 2019 Asian Sarcopenia Diagnosis Strategy. Physical measurements and fecal samples were collected, and 16S rRNA gene sequencing was conducted. Alpha and beta diversity analyses were performed to evaluate gut microbiota composition and diversity.Results: After HSCT, significant decreases in calf circumference and body mass index(BMI) were observed,accompanied by a decline in physical function. Gut microbiota analyses revealed significant differences in the relative abundance of Enterococcus, Bacteroides, Blautia and Dorea species before and after HSCT(P<0.05). Before HSCT, sarcopenic patients had lower Dorea levels and higher Phascolarctobacterium levels than non-sarcopenia patients(P<0.01). After HSCT, no significant differences in species abundance were observed. Alpha diversity analysis showed significant differences in species diversity among the groups, with the highest diversity in the postHSCT 90-day group and the lowest in the post-HSCT 30-day group. Beta diversity analysis revealed significant differences in species composition between pre-and post-HSCT time points but not between SP groups. Linear discriminant analysis effect size(LEfSe) identified Alistipes, Rikenellaceae, Alistipes putredinis, Prevotellaceae defectiva and Blautia coccoides as biomarkers for the pre-HSCT sarcopenia group. Functional predictions showed significant differences in anaerobic, biofilm-forming and oxidative stress-tolerant functions among the groups(P<0.05).Conclusions: This study demonstrated a significant decline in physical function after HSCT and identified potential gut microbiota biomarkers and functional alterations associated with SP in patients with hematological disorders. Further research is needed to explore the underlying mechanisms and potential therapeutic targets.展开更多
Ideal tissue-engineered scaffold materials regulate proliferation, apoptosis and differentiation of cells seeded on them by regulating gene expression. In this study, aligned and randomly oriented collagen nanofiber s...Ideal tissue-engineered scaffold materials regulate proliferation, apoptosis and differentiation of cells seeded on them by regulating gene expression. In this study, aligned and randomly oriented collagen nanofiber scaffolds were prepared using electronic spinning technology. Their diameters and appearance reached the standards of tissue-engineered nanometer scaffolds. The nanofiber scaffolds were characterized by a high swelling ratio, high porosity and good mechanical properties. The proliferation of spinal cord-derived neural stem cells on novel nanofiber scaffolds was obviously enhanced. The proportions of cells in the S and G2/M phases noticeably increased. Moreover, the proliferation rate of neural stem cells on the aligned collagen nanofiber scaffolds was high. The expression levels of cyclin D1 and cyclin-dependent kinase 2 were increased. Bcl-2 expression was significantly increased, but Bax and caspase-3 gene expressions were obviously decreased. There was no significant difference in the differentiation of neural stem cells into neurons on aligned and randomly oriented collagen nanofiber scaffolds. These results indicate that novel nanofiber scaffolds could promote the proliferation of spinal cord-derived neural stem cells and inhibit apoptosis without inducing differentiation. Nanofiber scaffolds regulate apoptosis and proliferation in neural stem cells by altering gene expression.展开更多
Numerous scientists are in the pursuit of energy storage materials with high energy and high power density by assembly of electrochemically active materials into conductive scaffolds,owing to the emerging need for nex...Numerous scientists are in the pursuit of energy storage materials with high energy and high power density by assembly of electrochemically active materials into conductive scaffolds,owing to the emerging need for next-generation energy storage devices.In this architectures,the active materials bonded to the conductive scaffold can provide a robust and free-standing structure,which is crucial to the fabrication of materials with high gravimetric capacity.Thus,hierarchical copper-cobalt-nickel ternary oxide(CuCoNi-oxide) nanowire arrays grown from copper foam were successfully fabricated as freestanding anode materials for lithium ion batteries(LIBs).CuCoNi-oxide nanowire arrays could provide more active sites owing to the hyperbranched structure,leading to a better specific capacity of 1191 mAh/g,cycle performance of 73% retention in comparison to CuO nanowire structure,which exhibited a specific capacity of 1029 mAh/g and capacity retention of 43%,respectively.展开更多
The thermodynamic properties of Mg Ca Si and its mother phase Ca2 Si are comparatively investigated from ab initio calculations and quasi-harmonic Debye-Grüneisen model. At 0 K, Mg Ca Si is more thermodynamically...The thermodynamic properties of Mg Ca Si and its mother phase Ca2 Si are comparatively investigated from ab initio calculations and quasi-harmonic Debye-Grüneisen model. At 0 K, Mg Ca Si is more thermodynamically stable. Under high temperature, the advantage of higher thermodynamically stability of Mg Ca Si is reduced, originating from the less negative entropy contribution because the thermodynamic entropy of Mg Ca Si increases more slowly with temperature and the entropy values are slightly smaller.With increasing temperature, the anti-softening ability for Mg Ca Si is slightly smaller due to the slightly faster decrease trend of bulk modulus than that of Ca2 Si, although the bulk modulus of Mg Ca Si is higher in the whole temperature range considered. The thermal expansion behaviors of both Mg Ca Si and Ca_(2)Si exhibit similar increase trend, although thermal expansion coefficient of MgCaSi is slightly lower and the increases is slightly slower at lower temperature. The isochoric heat capacity CVand isobaric heat capacity CPof MgCaSi and Ca_(2)Si rise nonlinearly with temperature, and both CVare close to the Dulong–Petit limit at high temperature due to the negligibly small electronic contribution. The Debye temperature of both phases decrease with increasing temperature, and the downtrend for Mg Ca Si is slightly faster.However, MgCaSi possess slightly higher Debye temperature, implying the stronger chemical bonds and higher thermal conductivity than the mother phase Ca_(2)Si. The Grüneisen parameter of MgCaSi and Ca_(2)Si increase slightly with temperature, the values of MgCaSi are slightly larger. The investigation of electronic structures shows that with substitution of partial Ca by Mg in Ca_(2)Si, the stronger MgASi,MgACa and SiASi covalent bonds are formed, and plays a very significant role for the structural stability and mechanical properties.展开更多
Dielectronic recombination(DR)is one of the dominant electron-ion recombination mechanisms for most highly charged ions(HCIs)in cosmic plasmas,and thus,it determines the charge state distribution and ionization balanc...Dielectronic recombination(DR)is one of the dominant electron-ion recombination mechanisms for most highly charged ions(HCIs)in cosmic plasmas,and thus,it determines the charge state distribution and ionization balance therein.To reliably interpret spectra from cosmic sources and model the astrophysical plasmas,precise DR rate coefficients are required to build up an accurate understanding of the ionization balance of the sources.The main cooler storage ring(CSRm)and the experimental cooler storage ring(CSRe)at the Heavy-Ion Research Facility in Lanzhou(HIRFL)are both equipped with electron cooling devices,which provide an excellent experimental platform for electron-ion collision studies for HCIs.Here,the status of the DR experiments at the HIRFL-CSR is outlined,and the DR measurements with Na-like Kr25^(+)ions at the CSRm and CSRe are taken as examples.In addition,the plasma recombination rate coefficients for Ar12^(+),14^(+),Ca14^(+),16^(+),17^(+),Ni19^(+),and Kr25^(+)ions obtained at the HIRFL-CSR are provided.All the data presented in this paper are openly available at https://doi.org/10.57760/sciencedb.j00113.00092.展开更多
Genetically modified oilseeds were used as processing raw material for edible oils. The protection of consumer rights to information as well as the genetically modified orgamisms （GMO） labe-ling polily required anal...Genetically modified oilseeds were used as processing raw material for edible oils. The protection of consumer rights to information as well as the genetically modified orgamisms （GMO） labe-ling polily required analytical methods to determine whether the oils contained genetically modified ingre-dient or not. Polymerase chain reaction （PCR） - based method was used commonly to determine the presence of GMOs. Adulteration attracted peoples concern also. Thus it was crucial that enough DNA ex-tracts can be obtained successfully from oil samples. For the purpose, three DNA extraction methods （modified emulsification method, the kits Wizard Magnetic DNA purification system for food and Nucleospin Food） ,were applied to 3 different grades of rapeseed oil samples. Those methods were compared by the amplification of Brassica napus reference gene CruA using real - time PCR. The results demonstra-ted that both the modified emulsification and the Nucleospin methods were able to extract enough DNA from refined oils. The modified emulsification method was superior to the kit of Nucleospin food due to smaller volume of required sample.展开更多
In order to boost power conversion efficiency(PCE) and operation stability of organic solar cells(OSCs),we propose a new idea of phase junction materials(PJMs) used as a photoactive layer component to improve device p...In order to boost power conversion efficiency(PCE) and operation stability of organic solar cells(OSCs),we propose a new idea of phase junction materials(PJMs) used as a photoactive layer component to improve device performance and stability.For this purpose,a novel PJM of H-TRC8 based on rhodanine unit was designed with a conjugated AH-D-A framework.Here,AH is a hydrogen-donating electron acceptor unit,D-A is an electron donor-acceptor unit.It is found that H-TRC8 has a good carriertransporting ability,as well as definite hydrogen-bond and D-A interaction with donor/acceptor materials.While H-TRC8 is added into the PBDB-T/PC60BM blend film with 1.0 vol% DIO(1,8-diiodooctane),the resulting blend film exhibited an enhanced absorption and improved morphology.The intermolecular hydrogen bond between H-TRC8 and PBDB-T plays an important role for them,which is confirmed via FT-IR spectra and 2D 1H NMR.As a result,the PBDB-T/PC60BM-based devices with 1.25 wt%H-TRC8 and 1.0 vol% DIO exhibit a significantly improved PCE of 8.06%,which is increased by 20.6% in comparison to that in the binary devices with 1.0 vol% DIO only(PCE=6.68%).Furthermore,the device stability is significantly enhanced with only 43% PCE roll-off at 150℃ for 120 h.This work indicates that AH-D-A-type PJMs are promising photovoltaic materials used as photoactive-layer components to achieve high-performance fullerene OSCs with high device stability.展开更多
BACKGROUND Epidermal growth factor receptor(EGFR)tyrosine kinase inhibitors(TKIs)have been adopted as the standard of care for non-small cell lung cancer(NSCLC)patients harboring EGFR sensitizing mutations.Besides the...BACKGROUND Epidermal growth factor receptor(EGFR)tyrosine kinase inhibitors(TKIs)have been adopted as the standard of care for non-small cell lung cancer(NSCLC)patients harboring EGFR sensitizing mutations.Besides the two common mutations exon 19 deletion and L858R,which together comprise approximately 85%of EGFR mutations in NSCLC,rare EGFR mutations also exist,including point mutations,deletions,and insertions spanning EGFR exons 18-25.However,the responsiveness of uncommon EGFR mutations to EGFR TKIs remains elusive and attracts increasing interest.CASE SUMMARY Herein,we report a 55-year-old male patient with stage IV NSCLC harboring a rare EGFR L833F-L861Q compound mutation in cis.The patient achieved a partial response to first-line treatment with afatinib and a progression-free survival of 10 mo.After afatinib failure,the patient received multiple line treatments with chemotherapy.Upon disease progression,the heavily pretreated patient was treated with osimertinib and bevacizumab,and both lung lesion and brain metastases were stable for more than 3 mo.He had an overall survival of 25 mo.CONCLUSION Our case revealed that both afatinib and the osimertinib+bevacizumab combination demonstrated clinical efficacy in NSCLC harboring an EGFR L833FL861Q compound mutation.The results provide more therapeutic options for patients with rare compound mutations.展开更多
To ensure food safety, it's vital to accurately detect genetically modified(GM)ingredient adulteration in food products and effectively detect the adulteration of vegetable oils from GM organisms(GMO). Therefore, ...To ensure food safety, it's vital to accurately detect genetically modified(GM)ingredient adulteration in food products and effectively detect the adulteration of vegetable oils from GM organisms(GMO). Therefore, it's essential to establish efficient DNA isolation method from vegetable oil. Here, we evaluate 5 DNA isolation methods using 25 commercial vegetable oils produced from soybean, peanut, corn, sunflower, rapeseed as well as blended oils. Quality of isolated DNA was determined by Nanodrop 2000 spectrophotometry. Real-time PCR and universal gene tRNA-Leu was used to assess resolution of methods. Our results showed that only DNA samples isolated by modified emulsification method based on cetyl trimethylammonium bromide(CTAB) were able to amplify t RNA-Leu gene.Moreover, Ct values of species specific endogenous reference genes were greater than 36 in these samples. In summary, CTAB method showed the best resolution on GMO adulteration detection for commercial vegetable oils, especially in fully refined oils.展开更多
基金supported by the Industry-Education Integration and Collaborative Education Project(No.230806121220054)the Joint Founding Project of Innovation Research Institute of Xijing Hospital(LHJJ24XL06).
文摘This study examined the role of moral disengagement dual system of self-control in the relationship between parental psychological control and cyberbullying.Participants were involved 802 vocational college students(46.01%females;M=18.11,SD=1.23).They completed measures on parental psychological control,moral disengagement,dual system of self-control(impulse and control system),and cyberbullying.The results from mediation-moderation analysis indicated that parental psychological control directly predicts higher cyberbullying.Specifically,moral disengagement partially mediated this relationship,as higher parental psychological control increases moral disengagement,which in turn elevates the risk of cyberbullying.Furthermore,parental psychological control moderated the relationship between parental control and cyberbullying through impulse control systems within the dual system of self-control.Individuals with high impulsivity scores are more likely to engage in cyberbullying when exposed to high levels of parental psychological control,whereas individuals with low impulsivity scores exhibit a lower incidence of cyberbullying.
基金by the National Natural Science Foundation of China(81571481 and 82060588)the Natural Science Foundation of Hubei Province,China(2017CFA017)+1 种基金the Wuhan Science and Technology Project(2019020701011444)the Medical Science Advancement Program of Wuhan University(TFJC2018001 and TFLC2018001).
文摘Epilepsy is a brain condition characterized by the recurrence of unprovoked seizures.Recent studies have shown that complement component 3(C3)aggravate the neuronal injury in epilepsy.And our previous studies revealed that TRPV1(transient receptor potential vanilloid type 1)is involved in epilepsy.Whether complement C3 regulation of neuronal injury is related to the activation of TRPV1 during epilepsy is not fully understood.We found that in a mouse model of status epilepticus(SE),complement C3 derived from astrocytes was increased and aggravated neuronal injury,and that TRPV 1-knockout rescued neurons from the injury induced by complement C3.Circular RNAs are abundant in the brain,and the reduction of circRad52 caused by complement C3 promoted the expression of TRPV 1 and exacerbated neuronal injury.Mechanistically,disorders of neuron-glia interaction mediated by the C3-TRPV1 signaling pathway may be important for the induction of neuronal injury.This study provides support for the hypothesis that the C3-TRFV1 pathway is involved in the prevention and treatment of neuronal injury and cognitive disorders.
基金supported by Grant National Key R&D Program of China (No.2020YFC2005600 and No.2020YFC2005605)。
文摘Objective: The aim of this study was to investigate the prevalence of sarcopenia(SP) and its relationship with gut microbiota alterations in patients with hematological diseases before and after hematopoietic stem cell transplantation(HSCT).Methods: A total of 108 patients with various hematological disorders were selected from Peking University People’s Hospital. SP was screened and diagnosed based on the 2019 Asian Sarcopenia Diagnosis Strategy. Physical measurements and fecal samples were collected, and 16S rRNA gene sequencing was conducted. Alpha and beta diversity analyses were performed to evaluate gut microbiota composition and diversity.Results: After HSCT, significant decreases in calf circumference and body mass index(BMI) were observed,accompanied by a decline in physical function. Gut microbiota analyses revealed significant differences in the relative abundance of Enterococcus, Bacteroides, Blautia and Dorea species before and after HSCT(P<0.05). Before HSCT, sarcopenic patients had lower Dorea levels and higher Phascolarctobacterium levels than non-sarcopenia patients(P<0.01). After HSCT, no significant differences in species abundance were observed. Alpha diversity analysis showed significant differences in species diversity among the groups, with the highest diversity in the postHSCT 90-day group and the lowest in the post-HSCT 30-day group. Beta diversity analysis revealed significant differences in species composition between pre-and post-HSCT time points but not between SP groups. Linear discriminant analysis effect size(LEfSe) identified Alistipes, Rikenellaceae, Alistipes putredinis, Prevotellaceae defectiva and Blautia coccoides as biomarkers for the pre-HSCT sarcopenia group. Functional predictions showed significant differences in anaerobic, biofilm-forming and oxidative stress-tolerant functions among the groups(P<0.05).Conclusions: This study demonstrated a significant decline in physical function after HSCT and identified potential gut microbiota biomarkers and functional alterations associated with SP in patients with hematological disorders. Further research is needed to explore the underlying mechanisms and potential therapeutic targets.
文摘Ideal tissue-engineered scaffold materials regulate proliferation, apoptosis and differentiation of cells seeded on them by regulating gene expression. In this study, aligned and randomly oriented collagen nanofiber scaffolds were prepared using electronic spinning technology. Their diameters and appearance reached the standards of tissue-engineered nanometer scaffolds. The nanofiber scaffolds were characterized by a high swelling ratio, high porosity and good mechanical properties. The proliferation of spinal cord-derived neural stem cells on novel nanofiber scaffolds was obviously enhanced. The proportions of cells in the S and G2/M phases noticeably increased. Moreover, the proliferation rate of neural stem cells on the aligned collagen nanofiber scaffolds was high. The expression levels of cyclin D1 and cyclin-dependent kinase 2 were increased. Bcl-2 expression was significantly increased, but Bax and caspase-3 gene expressions were obviously decreased. There was no significant difference in the differentiation of neural stem cells into neurons on aligned and randomly oriented collagen nanofiber scaffolds. These results indicate that novel nanofiber scaffolds could promote the proliferation of spinal cord-derived neural stem cells and inhibit apoptosis without inducing differentiation. Nanofiber scaffolds regulate apoptosis and proliferation in neural stem cells by altering gene expression.
基金financially supported by the National Natural Science Foundation of China (No.21975163)China Postdoctoral Science Foundation (No.2018M633125)。
文摘Numerous scientists are in the pursuit of energy storage materials with high energy and high power density by assembly of electrochemically active materials into conductive scaffolds,owing to the emerging need for next-generation energy storage devices.In this architectures,the active materials bonded to the conductive scaffold can provide a robust and free-standing structure,which is crucial to the fabrication of materials with high gravimetric capacity.Thus,hierarchical copper-cobalt-nickel ternary oxide(CuCoNi-oxide) nanowire arrays grown from copper foam were successfully fabricated as freestanding anode materials for lithium ion batteries(LIBs).CuCoNi-oxide nanowire arrays could provide more active sites owing to the hyperbranched structure,leading to a better specific capacity of 1191 mAh/g,cycle performance of 73% retention in comparison to CuO nanowire structure,which exhibited a specific capacity of 1029 mAh/g and capacity retention of 43%,respectively.
基金support from Significant Project of Guangxi Scientific Foundation (2018GXNSFDA281010)National Natural Science Foundation of China (51461002)。
文摘The thermodynamic properties of Mg Ca Si and its mother phase Ca2 Si are comparatively investigated from ab initio calculations and quasi-harmonic Debye-Grüneisen model. At 0 K, Mg Ca Si is more thermodynamically stable. Under high temperature, the advantage of higher thermodynamically stability of Mg Ca Si is reduced, originating from the less negative entropy contribution because the thermodynamic entropy of Mg Ca Si increases more slowly with temperature and the entropy values are slightly smaller.With increasing temperature, the anti-softening ability for Mg Ca Si is slightly smaller due to the slightly faster decrease trend of bulk modulus than that of Ca2 Si, although the bulk modulus of Mg Ca Si is higher in the whole temperature range considered. The thermal expansion behaviors of both Mg Ca Si and Ca_(2)Si exhibit similar increase trend, although thermal expansion coefficient of MgCaSi is slightly lower and the increases is slightly slower at lower temperature. The isochoric heat capacity CVand isobaric heat capacity CPof MgCaSi and Ca_(2)Si rise nonlinearly with temperature, and both CVare close to the Dulong–Petit limit at high temperature due to the negligibly small electronic contribution. The Debye temperature of both phases decrease with increasing temperature, and the downtrend for Mg Ca Si is slightly faster.However, MgCaSi possess slightly higher Debye temperature, implying the stronger chemical bonds and higher thermal conductivity than the mother phase Ca_(2)Si. The Grüneisen parameter of MgCaSi and Ca_(2)Si increase slightly with temperature, the values of MgCaSi are slightly larger. The investigation of electronic structures shows that with substitution of partial Ca by Mg in Ca_(2)Si, the stronger MgASi,MgACa and SiASi covalent bonds are formed, and plays a very significant role for the structural stability and mechanical properties.
基金supported by the National Natural Science Foundation of China (Grant Nos. U1932207, 11904371, and 12104437)the Strategic Priority Research Program of Chinese Academy of Sciences (Grant No. XDB34020000)
文摘Dielectronic recombination(DR)is one of the dominant electron-ion recombination mechanisms for most highly charged ions(HCIs)in cosmic plasmas,and thus,it determines the charge state distribution and ionization balance therein.To reliably interpret spectra from cosmic sources and model the astrophysical plasmas,precise DR rate coefficients are required to build up an accurate understanding of the ionization balance of the sources.The main cooler storage ring(CSRm)and the experimental cooler storage ring(CSRe)at the Heavy-Ion Research Facility in Lanzhou(HIRFL)are both equipped with electron cooling devices,which provide an excellent experimental platform for electron-ion collision studies for HCIs.Here,the status of the DR experiments at the HIRFL-CSR is outlined,and the DR measurements with Na-like Kr25^(+)ions at the CSRm and CSRe are taken as examples.In addition,the plasma recombination rate coefficients for Ar12^(+),14^(+),Ca14^(+),16^(+),17^(+),Ni19^(+),and Kr25^(+)ions obtained at the HIRFL-CSR are provided.All the data presented in this paper are openly available at https://doi.org/10.57760/sciencedb.j00113.00092.
文摘Genetically modified oilseeds were used as processing raw material for edible oils. The protection of consumer rights to information as well as the genetically modified orgamisms （GMO） labe-ling polily required analytical methods to determine whether the oils contained genetically modified ingre-dient or not. Polymerase chain reaction （PCR） - based method was used commonly to determine the presence of GMOs. Adulteration attracted peoples concern also. Thus it was crucial that enough DNA ex-tracts can be obtained successfully from oil samples. For the purpose, three DNA extraction methods （modified emulsification method, the kits Wizard Magnetic DNA purification system for food and Nucleospin Food） ,were applied to 3 different grades of rapeseed oil samples. Those methods were compared by the amplification of Brassica napus reference gene CruA using real - time PCR. The results demonstra-ted that both the modified emulsification and the Nucleospin methods were able to extract enough DNA from refined oils. The modified emulsification method was superior to the kit of Nucleospin food due to smaller volume of required sample.
基金financial support from the National Natural Science Foundation of China(Nos.51673031,51573154)the Major Program of the Natural Science Research of Jiangsu Higher Education Institutions(No.18KJA480001)+3 种基金the Top-Notch Academic Programs Project(TAPP)for Polymeric Materials Science and Technologythe Priority Academic Program Development(PAPD)of Jiangsu Higher Education InstitutionsJiangsu Provincial Talents Project of High-Level Innovation and Entrepreneurshipthe Talent Project of Jiangsu Specially-Appointed Professor。
文摘In order to boost power conversion efficiency(PCE) and operation stability of organic solar cells(OSCs),we propose a new idea of phase junction materials(PJMs) used as a photoactive layer component to improve device performance and stability.For this purpose,a novel PJM of H-TRC8 based on rhodanine unit was designed with a conjugated AH-D-A framework.Here,AH is a hydrogen-donating electron acceptor unit,D-A is an electron donor-acceptor unit.It is found that H-TRC8 has a good carriertransporting ability,as well as definite hydrogen-bond and D-A interaction with donor/acceptor materials.While H-TRC8 is added into the PBDB-T/PC60BM blend film with 1.0 vol% DIO(1,8-diiodooctane),the resulting blend film exhibited an enhanced absorption and improved morphology.The intermolecular hydrogen bond between H-TRC8 and PBDB-T plays an important role for them,which is confirmed via FT-IR spectra and 2D 1H NMR.As a result,the PBDB-T/PC60BM-based devices with 1.25 wt%H-TRC8 and 1.0 vol% DIO exhibit a significantly improved PCE of 8.06%,which is increased by 20.6% in comparison to that in the binary devices with 1.0 vol% DIO only(PCE=6.68%).Furthermore,the device stability is significantly enhanced with only 43% PCE roll-off at 150℃ for 120 h.This work indicates that AH-D-A-type PJMs are promising photovoltaic materials used as photoactive-layer components to achieve high-performance fullerene OSCs with high device stability.
文摘BACKGROUND Epidermal growth factor receptor(EGFR)tyrosine kinase inhibitors(TKIs)have been adopted as the standard of care for non-small cell lung cancer(NSCLC)patients harboring EGFR sensitizing mutations.Besides the two common mutations exon 19 deletion and L858R,which together comprise approximately 85%of EGFR mutations in NSCLC,rare EGFR mutations also exist,including point mutations,deletions,and insertions spanning EGFR exons 18-25.However,the responsiveness of uncommon EGFR mutations to EGFR TKIs remains elusive and attracts increasing interest.CASE SUMMARY Herein,we report a 55-year-old male patient with stage IV NSCLC harboring a rare EGFR L833F-L861Q compound mutation in cis.The patient achieved a partial response to first-line treatment with afatinib and a progression-free survival of 10 mo.After afatinib failure,the patient received multiple line treatments with chemotherapy.Upon disease progression,the heavily pretreated patient was treated with osimertinib and bevacizumab,and both lung lesion and brain metastases were stable for more than 3 mo.He had an overall survival of 25 mo.CONCLUSION Our case revealed that both afatinib and the osimertinib+bevacizumab combination demonstrated clinical efficacy in NSCLC harboring an EGFR L833FL861Q compound mutation.The results provide more therapeutic options for patients with rare compound mutations.
基金supported by grants from the National Major Special Project for the Development of Transgenic Organisms (grant No. 2016ZX08012-005, 2016ZX08012-003)the National Natural Science Foundation of China (31601581)
文摘To ensure food safety, it's vital to accurately detect genetically modified(GM)ingredient adulteration in food products and effectively detect the adulteration of vegetable oils from GM organisms(GMO). Therefore, it's essential to establish efficient DNA isolation method from vegetable oil. Here, we evaluate 5 DNA isolation methods using 25 commercial vegetable oils produced from soybean, peanut, corn, sunflower, rapeseed as well as blended oils. Quality of isolated DNA was determined by Nanodrop 2000 spectrophotometry. Real-time PCR and universal gene tRNA-Leu was used to assess resolution of methods. Our results showed that only DNA samples isolated by modified emulsification method based on cetyl trimethylammonium bromide(CTAB) were able to amplify t RNA-Leu gene.Moreover, Ct values of species specific endogenous reference genes were greater than 36 in these samples. In summary, CTAB method showed the best resolution on GMO adulteration detection for commercial vegetable oils, especially in fully refined oils.
