OBJECTIVE:To examine the efficacy of Qinghuayin(清化饮,QHY)in rat chronic atrophic gastritis(CAG)models and explored the molecular mechanism of QHY in treating CAG.METHODS:In total,65 Wistar rats were randomly divided...OBJECTIVE:To examine the efficacy of Qinghuayin(清化饮,QHY)in rat chronic atrophic gastritis(CAG)models and explored the molecular mechanism of QHY in treating CAG.METHODS:In total,65 Wistar rats were randomly divided into the control(n=10)and CAG groups(n=55).CAG model rats were further divided into five groups:model(n=10),vitacoenzyme(n=10),low-dose QHY(n=10),medium-dose QHY(n=10),and high-dose QHY groups(n=10).We analyzed histopathological changes using hematoxylin and eosin staining and measured interleukin(IL)-6 and IL-8 levels in serum using enzyme-linked immunosorbent assay(ELISA)(Boster Bio,Pleasanton,USA).In addition,gastrin(GAS),pepsinogen I(PGI),and PGII expressions were evaluated using ELISA.The protein and m RNA expression of toll-like receptor 4(TLR4)and toll or interleukin-1 receptor domaincontaining adaptor inducing interferon-β(TRIF)was detected by Western blotting and quantitative reverse transcription-polymerase chain reaction,respectively.RESULTS:Our results revealed that histopathological changes in CAG model rates could be restored by low-,medium-,and high-dose QHY.The changes in GAS and PGI/II expression demonstrated that QHY improved CAG.Serum IL-6 and IL-levels were decreased by QHY administration.TLR4 and TRIF were upregulated at the m RNA and protein levels in the model group but downregulated by QHY administration.CONCLUSION:We concluded that QHY could effectively improve the histopathological changes of the gastric mucosa induced by CAG in rats.The therapeutic mechanism of QHY may be related to inhibition of the inflammatory factors IL-6 and IL-8 and suppression of TLR4/TRIF m RNA and protein expression.展开更多
With enormous growth of the number of Internet users and appearance of new applications, characterization of Internet traffic has attracted more and more attention and has become one of the major challenging issues in...With enormous growth of the number of Internet users and appearance of new applications, characterization of Internet traffic has attracted more and more attention and has become one of the major challenging issues in telecommunication network over the past few years. In this paper, we study the network traffic pattern of the aggregate traffic and of specific application traffic, especially the popular applications such as P2P, VoIP that contribute most network traffic. Our study verified that majority Internet backbone traffic is contributed by a small portion of users and a power function can be used to approximate the contribution of each user to the overall traffic. We show that P2P applications are the dominant traffic contributor in current Internet Backbone of China. In addition, we selectively present the traffic pattern of different applications in detail.展开更多
In this study, the homotopy analysis method (HAM) is used to solve the generalized Duffing equation. Both the frequencies and periodic solutions of the nonlinear Duffing equation can be explicitly and analytically for...In this study, the homotopy analysis method (HAM) is used to solve the generalized Duffing equation. Both the frequencies and periodic solutions of the nonlinear Duffing equation can be explicitly and analytically formulated. Accuracy and validity of the proposed techniques are then verified by comparing the numerical results obtained based on the HAM and numerical integration method. Numerical simulations are extended for even very strong nonlinearities and very good correlations which achieved between the results. Besides, the optimal HAM approach is introduced to accelerate the convergence of solutions.展开更多
基金Supported by Natural Science Foundation of Fujian Province(Based on NLRP3 Inflammatory Body/Caspase-1-mediated Gastric Epithelial Cell Death to Explore the Molecular Mechanism of Qinghua Decoction in the Treatment of Chronic Atrophic Gastritis,No.2020J01253)。
文摘OBJECTIVE:To examine the efficacy of Qinghuayin(清化饮,QHY)in rat chronic atrophic gastritis(CAG)models and explored the molecular mechanism of QHY in treating CAG.METHODS:In total,65 Wistar rats were randomly divided into the control(n=10)and CAG groups(n=55).CAG model rats were further divided into five groups:model(n=10),vitacoenzyme(n=10),low-dose QHY(n=10),medium-dose QHY(n=10),and high-dose QHY groups(n=10).We analyzed histopathological changes using hematoxylin and eosin staining and measured interleukin(IL)-6 and IL-8 levels in serum using enzyme-linked immunosorbent assay(ELISA)(Boster Bio,Pleasanton,USA).In addition,gastrin(GAS),pepsinogen I(PGI),and PGII expressions were evaluated using ELISA.The protein and m RNA expression of toll-like receptor 4(TLR4)and toll or interleukin-1 receptor domaincontaining adaptor inducing interferon-β(TRIF)was detected by Western blotting and quantitative reverse transcription-polymerase chain reaction,respectively.RESULTS:Our results revealed that histopathological changes in CAG model rates could be restored by low-,medium-,and high-dose QHY.The changes in GAS and PGI/II expression demonstrated that QHY improved CAG.Serum IL-6 and IL-levels were decreased by QHY administration.TLR4 and TRIF were upregulated at the m RNA and protein levels in the model group but downregulated by QHY administration.CONCLUSION:We concluded that QHY could effectively improve the histopathological changes of the gastric mucosa induced by CAG in rats.The therapeutic mechanism of QHY may be related to inhibition of the inflammatory factors IL-6 and IL-8 and suppression of TLR4/TRIF m RNA and protein expression.
文摘With enormous growth of the number of Internet users and appearance of new applications, characterization of Internet traffic has attracted more and more attention and has become one of the major challenging issues in telecommunication network over the past few years. In this paper, we study the network traffic pattern of the aggregate traffic and of specific application traffic, especially the popular applications such as P2P, VoIP that contribute most network traffic. Our study verified that majority Internet backbone traffic is contributed by a small portion of users and a power function can be used to approximate the contribution of each user to the overall traffic. We show that P2P applications are the dominant traffic contributor in current Internet Backbone of China. In addition, we selectively present the traffic pattern of different applications in detail.
文摘In this study, the homotopy analysis method (HAM) is used to solve the generalized Duffing equation. Both the frequencies and periodic solutions of the nonlinear Duffing equation can be explicitly and analytically formulated. Accuracy and validity of the proposed techniques are then verified by comparing the numerical results obtained based on the HAM and numerical integration method. Numerical simulations are extended for even very strong nonlinearities and very good correlations which achieved between the results. Besides, the optimal HAM approach is introduced to accelerate the convergence of solutions.
