Fos-related antigen 1(Fra-1)is a nuclear transcription factor that regulates cell growth,differentiation,and apoptosis.It is involved in the proliferation,invasion,apoptosis and epithelial mesenchymal transformation o...Fos-related antigen 1(Fra-1)is a nuclear transcription factor that regulates cell growth,differentiation,and apoptosis.It is involved in the proliferation,invasion,apoptosis and epithelial mesenchymal transformation of malignant tumor cells.Fra-1 is highly expressed in gastric cancer(GC),affects the cycle distribution and apoptosis of GC cells,and participates in GC occurrence and development.However,the detailed mechanism of Fra-1 in GC is unclear,such as the identification of Fra-1-interacting proteins and their role in GC pathogenesis.In this study,we identified tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta(YWHAH)as a Fra-1-interacting protein in GC cells using co-immunoprecipitation combined with liquid chromatography-tandem mass spectrometry.Experiments showed that YWHAH positively regulated Fra-1 mRNA and protein expression,and affected GC cell proliferation.Whole proteome analysis showed that Fra-1 affected the activity of the high mobility group AT-hook 1(HMGA1)/phosphatidylinositol-4,5-bisphosphate 3-kinase(PI3K)/protein kinase B(AKT)/mechanistic target of rapamycin(mTOR)signaling pathway in GC cells.Western blotting and flow cytometry confirmed that YWHAH activated HMGA1/PI3K/AKT/mTOR signaling pathway by positively regulating Fra-1 to affect GC cell proliferation.These results will help to discover new molecular targets for the early diagnosis,treatment,and prognosis prediction of GC.展开更多
[目的]比较“肩-肩”新型解剖标记定位法与人工智能辅助规划系统(AIHIP系统)规划的全髋关节置换(total hip arthroplasty, THA)中假体置入定位及预防双下肢不等长的准确性。[方法] 2020年8月-2022年12月就诊于本院行初次THA的94例患者,...[目的]比较“肩-肩”新型解剖标记定位法与人工智能辅助规划系统(AIHIP系统)规划的全髋关节置换(total hip arthroplasty, THA)中假体置入定位及预防双下肢不等长的准确性。[方法] 2020年8月-2022年12月就诊于本院行初次THA的94例患者,按照随机数字表法分为两组,47例采用“肩-肩”法(肩-肩组),另外47例采用AIHIP系统规划法(智能组),分别于术前进行规划,比较两组临床和影像结果。[结果]两组患者手术时间、总失血量、术后下地时间的差异均无统计学意义(P>0.05)。影像方面,两组髋臼假体匹配、股骨柄假体匹配、双下肢长度差、联合偏心距的差异均无统计学意义(P>0.05),肩-肩组的尖肩距[(15.9±3.0) mm vs(17.7±3.4) mm, P<0.001]、截骨距[(7.8±2.7) mm vs(9.2±4.1) mm, P=0.003]均显著小于智能组。[结论]“肩-肩”新型解剖标记定位法可以在THA术中准确置入股骨侧假体,并可预防术后双下肢不等长。展开更多
基金This work was supported by the Hunan Provincial Natural Science Foundation(2021JJ30915).
文摘Fos-related antigen 1(Fra-1)is a nuclear transcription factor that regulates cell growth,differentiation,and apoptosis.It is involved in the proliferation,invasion,apoptosis and epithelial mesenchymal transformation of malignant tumor cells.Fra-1 is highly expressed in gastric cancer(GC),affects the cycle distribution and apoptosis of GC cells,and participates in GC occurrence and development.However,the detailed mechanism of Fra-1 in GC is unclear,such as the identification of Fra-1-interacting proteins and their role in GC pathogenesis.In this study,we identified tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta(YWHAH)as a Fra-1-interacting protein in GC cells using co-immunoprecipitation combined with liquid chromatography-tandem mass spectrometry.Experiments showed that YWHAH positively regulated Fra-1 mRNA and protein expression,and affected GC cell proliferation.Whole proteome analysis showed that Fra-1 affected the activity of the high mobility group AT-hook 1(HMGA1)/phosphatidylinositol-4,5-bisphosphate 3-kinase(PI3K)/protein kinase B(AKT)/mechanistic target of rapamycin(mTOR)signaling pathway in GC cells.Western blotting and flow cytometry confirmed that YWHAH activated HMGA1/PI3K/AKT/mTOR signaling pathway by positively regulating Fra-1 to affect GC cell proliferation.These results will help to discover new molecular targets for the early diagnosis,treatment,and prognosis prediction of GC.
文摘[目的]比较“肩-肩”新型解剖标记定位法与人工智能辅助规划系统(AIHIP系统)规划的全髋关节置换(total hip arthroplasty, THA)中假体置入定位及预防双下肢不等长的准确性。[方法] 2020年8月-2022年12月就诊于本院行初次THA的94例患者,按照随机数字表法分为两组,47例采用“肩-肩”法(肩-肩组),另外47例采用AIHIP系统规划法(智能组),分别于术前进行规划,比较两组临床和影像结果。[结果]两组患者手术时间、总失血量、术后下地时间的差异均无统计学意义(P>0.05)。影像方面,两组髋臼假体匹配、股骨柄假体匹配、双下肢长度差、联合偏心距的差异均无统计学意义(P>0.05),肩-肩组的尖肩距[(15.9±3.0) mm vs(17.7±3.4) mm, P<0.001]、截骨距[(7.8±2.7) mm vs(9.2±4.1) mm, P=0.003]均显著小于智能组。[结论]“肩-肩”新型解剖标记定位法可以在THA术中准确置入股骨侧假体,并可预防术后双下肢不等长。
