Pigs have emerged as valuable large-animal models for cardiac xenotransplantation;however,the temporal dynamics of myocardial development in this species remains insufficiently defined.This study analyzed gene express...Pigs have emerged as valuable large-animal models for cardiac xenotransplantation;however,the temporal dynamics of myocardial development in this species remains insufficiently defined.This study analyzed gene expression patterns across four key developmental stages(neonatal,juvenile,sexual maturity,and adulthood)to delineate the molecular mechanisms driving porcine myocardial development.Increases in heart weight were accompanied by proportional expansion of myocardial fiber area and chamber size,reflecting coordinated structural development.Transcriptomic profiling of myocardial tissue by RNA sequencing(RNA-seq)identified 2189 differentially expressed genes(DEGs)across stage comparisons.Short time-series expression miner(STEM)analysis classified these DEGs into four major expression clusters enriched in pathways associated with myocardial development,immune responses,cell proliferation,and metabolic processes.Among 359 DEGs conserved across all developmental stages,six candidate genes were strongly associated with myocardial development.Reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR)confirmed a significant correlation between the expression of these candidate genes and myocardial development in porcine tissue.These findings establish a transcriptomic framework for porcine myocardial maturation and provide a molecular basis for advancing cardiac xenotransplantation.展开更多
OBJECTIVE:To evaluate the efficacy and safety of Qingchang suppository(清肠栓,QCS),a preparation of Chinese herbal medicine,in the induction of remission in patients with mild-to-moderate ulcerative proctitis(UP).METH...OBJECTIVE:To evaluate the efficacy and safety of Qingchang suppository(清肠栓,QCS),a preparation of Chinese herbal medicine,in the induction of remission in patients with mild-to-moderate ulcerative proctitis(UP).METHODS:We performed a multicenter,prospective,randomized,parallel-controlled trial to evaluate the efficacy of QCS induction therapy in 140 adult patients with mild-to-moderate UP and TCM syndrome of dampness-heat in large intestine.The patients were randomized to receive QCS(study group)or Salicylazosulfapyridine(SASP)suppository(control group)one piece each time,twice a day,per anum for 12 weeks.Mayo score and main symptoms score were evaluated at weeks 0,2,4,8 and 12,rectosigmoidscopy was taken at weeks 0,4,8 and 12,Geboes score,erythrocyte sedimentation rate(ESR),C-reactive protein(CRP)and safety indexes were assessed at weeks 0 and 12.The primary efficacy endpoint is clinical remission rate,the secondary efficacy endpoints are clinical response rate,mucosa healing rate,Geboes score,the remission rates of the main symptoms,the median day to the remission of the symptom,etc.RESULTS:There were no statistical difference in the clinical remission rates,the clinical response rates,the mucosa healing rates,Geboes score,ESR and CRP between the two groups.The remission rates of tenesmus and anal burning sensation of the study group were significantly higher than those of the control group(76.5%vs 25.0%,P=0.009;74.51%vs 29.63%,P=0.003).The median day to the remission of purulent bloody stool of the study group was significantly less than that of control group[11(1,64)vs 19(2,67),P=0.007].The patients receiving QCS had a significantly higher mucosa healing rate at week 4 than the patients receiving SASP suppository(71.42%vs 52.85%,P=0.023).No adverse event occurred in the study group while the adverse events incidence of the control group was 5.7%(P=0.049).CONCLUSIONS:QCS could induce the remission of UP as effectively and safely as SASP suppository,and was superior to SASP suppository in relieving the symptoms of tenesmus,anal burning sensation and purulent bloody stool and the time to reach mucosa healing.展开更多
目的:评估超声内镜引导下细针穿刺(endoscopic ultrasound-guided fine needle aspiration,EUS-FNA)在胆管病变中的诊断效能和安全性,并探讨影响其准确性的因素。方法:回顾性分析2021年1月到2023年12月成都市第三人民医院因疑似胆管恶...目的:评估超声内镜引导下细针穿刺(endoscopic ultrasound-guided fine needle aspiration,EUS-FNA)在胆管病变中的诊断效能和安全性,并探讨影响其准确性的因素。方法:回顾性分析2021年1月到2023年12月成都市第三人民医院因疑似胆管恶性狭窄/占位而接受EUS-FNA的41例患者临床资料,其中男性22例,女性19例,平均年龄为65.9(35.0~89.0)岁。评估其诊断效能(敏感性、特异性、阳性预测值、阴性预测值和准确性)及影响因素。结果:EUS-FNA对于胆管病变的总体灵敏性为85%,特异性为100%,阳性预测值为100%,阴性预测值为33%,准确性为86%;25G穿刺针和是否存在实性占位是影响EUS-FNA诊断胆管病变准确性的重要因素,而穿刺部位并未对诊断效能产生影响,参与分析的41例患者在随访期间未发现与EUS-FNA相关不良事件。结论:EUS-FNA在诊断胆管病变时具有较高的准确性和安全性,特别是使用25G穿刺针及存在胆管实性占位时,EUSFNA的诊断准确性更高。展开更多
系统梳理中医药干预溃疡性结肠炎(UC)的随机对照试验(RCT),分析中医药临床试验的研究特征和结局指标,以期为后续开展中医药干预UC的RCT设计提供参考,为中医药在UC中的临床应用提供证据支持。计算机检索中国知网(CNKI)、万方(Wanfang)、...系统梳理中医药干预溃疡性结肠炎(UC)的随机对照试验(RCT),分析中医药临床试验的研究特征和结局指标,以期为后续开展中医药干预UC的RCT设计提供参考,为中医药在UC中的临床应用提供证据支持。计算机检索中国知网(CNKI)、万方(Wanfang)、维普(VIP)、中国生物医学文献服务系统(SinoMed)、PubMed、Cochrane Library、EMbase、Web of Science数据库2021年1月至2024年8月中医药治疗UC的RCT,评估偏倚风险,定性分析结局指标。最终纳入555篇RCT,样本量44853例,样本量最大为218例,最小为28例,多集中在60~100例;386篇RCT明确报告了中医证型,排序前3的分别是大肠湿热证(31.05%)、脾肾阳虚证(12.47%)及脾虚湿蕴证(9.17%);干预措施使用频数由高到低包括内服中药复方/制剂(64.5%)、中药复方/制剂保留灌肠(18.2%)、内服中药复方/制剂+中医外治(5.95%)、中医外治(4.86%)等;治疗疗程多集中在4~8周(64.86%),61篇(10.99%)研究报告了随访时间;共使用了157种结局指标,使用频数3460次,分属6个指标域:中医证候/症状(346次,10%)、症状/体征(541次,15.64%)、理化检查(2119次,61.24%)、生活质量(107次,3.09%)、远期预后(61次,1.76%)、安全性事件(284次,8.21%)。结果表明,中医药治疗UC的结局指标面临样本量计算缺乏依据、中医辨证分型欠规范、试验方案的设计与注册缺失、盲法与分配隐藏不足、干预措施的依从性、替代指标与终点指标选择不平衡、结局指标测量时点不统一以及设计待规范、伦理与安全性问题的局限性。建议未来应积极构建一套涵盖中医证型的标准化分类、明确的疗效评价指标、关键的终点指标及合理的测量时点,同时强调对远期预后影响的追踪、患者生活质量的全面评估及经济学效益考量的UC核心指标集,为中医药治疗UC的临床实践提供依据。展开更多
Accurate short-term wind power forecast technique plays a crucial role in maintaining the safety and economic efficiency of smart grids.Although numerous studies have employed various methods to forecast wind power,th...Accurate short-term wind power forecast technique plays a crucial role in maintaining the safety and economic efficiency of smart grids.Although numerous studies have employed various methods to forecast wind power,there remains a research gap in leveraging swarm intelligence algorithms to optimize the hyperparameters of the Transformer model for wind power prediction.To improve the accuracy of short-term wind power forecast,this paper proposes a hybrid short-term wind power forecast approach named STL-IAOA-iTransformer,which is based on seasonal and trend decomposition using LOESS(STL)and iTransformer model optimized by improved arithmetic optimization algorithm(IAOA).First,to fully extract the power data features,STL is used to decompose the original data into components with less redundant information.The extracted components as well as the weather data are then input into iTransformer for short-term wind power forecast.The final predicted short-term wind power curve is obtained by combining the predicted components.To improve the model accuracy,IAOA is employed to optimize the hyperparameters of iTransformer.The proposed approach is validated using real-generation data from different seasons and different power stations inNorthwest China,and ablation experiments have been conducted.Furthermore,to validate the superiority of the proposed approach under different wind characteristics,real power generation data fromsouthwestChina are utilized for experiments.Thecomparative results with the other six state-of-the-art prediction models in experiments show that the proposed model well fits the true value of generation series and achieves high prediction accuracy.展开更多
The Early Cambrian Yuertusi Formation(Є_(1)y)in the Tarim Basin of China deposits a continuously developed suite of organic-rich black mudstones,which constitute an important source of oil and gas reservoirs in the Pa...The Early Cambrian Yuertusi Formation(Є_(1)y)in the Tarim Basin of China deposits a continuously developed suite of organic-rich black mudstones,which constitute an important source of oil and gas reservoirs in the Paleozoic.However,its hydrocarbon generation and evolution characteristics and resource potential have long been constrained by deeply buried strata and previous research.In this paper,based on the newly obtained ultra-deep well drilling data,the hydrocarbon generation and expulsion model ofЄ_(1)y shale was established by using data-driven Monte Carlo simulation,upon which the hydrocarbon generation,expulsion,and retention amounts were calculated by using the diagenetic method.The research indicates that theЄ_(1)y shale reaches the hydrocarbon generation and expulsion threshold at equivalent vitrinite reflectances of 0.46%and 0.72%,respectively.The cumulative hydrocarbon generation is 68.88×10^(10)t,the cumulative hydrocarbon expulsion is 35.59×10^(10)t,and the cumulative residual hydrocarbon is 33.29×10^(10)t.This paper systematically and quantitatively calculates the hydrocarbon expulsion at various key geological periods for theЄ_(1)y source rocks in the study area for the first time,more precisely confirming that the black shale of theЄ_(1)y is the most significant source rock contributing to the marine oil and gas resources in the Tarim Basin,filling the gap in hydrocarbon expulsion calculation in the study area,and providing an important basis for the formation and distribution of Paleozoic hydrocarbon reservoirs.The prospect of deep ultra-deep oil and gas exploration in the Tarim Basin is promising.Especially,the large area of dolomite reservoirs under the Cambrian salt and source rock interiors are the key breakthrough targets for the next exploration in the Tarim Basin.展开更多
基金supported by the National Natural Science Foundation of China(32222079 to L.J.and 32202621 to D.D.W.)。
文摘Pigs have emerged as valuable large-animal models for cardiac xenotransplantation;however,the temporal dynamics of myocardial development in this species remains insufficiently defined.This study analyzed gene expression patterns across four key developmental stages(neonatal,juvenile,sexual maturity,and adulthood)to delineate the molecular mechanisms driving porcine myocardial development.Increases in heart weight were accompanied by proportional expansion of myocardial fiber area and chamber size,reflecting coordinated structural development.Transcriptomic profiling of myocardial tissue by RNA sequencing(RNA-seq)identified 2189 differentially expressed genes(DEGs)across stage comparisons.Short time-series expression miner(STEM)analysis classified these DEGs into four major expression clusters enriched in pathways associated with myocardial development,immune responses,cell proliferation,and metabolic processes.Among 359 DEGs conserved across all developmental stages,six candidate genes were strongly associated with myocardial development.Reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR)confirmed a significant correlation between the expression of these candidate genes and myocardial development in porcine tissue.These findings establish a transcriptomic framework for porcine myocardial maturation and provide a molecular basis for advancing cardiac xenotransplantation.
基金Shanghai Municipal Administration of Traditional Chinese Medicine:Leadship Training Program of Inheritance and Innovation Team of Shanghai School of Traditional Chinese Medicine(No.2021WXGLP-007)。
文摘OBJECTIVE:To evaluate the efficacy and safety of Qingchang suppository(清肠栓,QCS),a preparation of Chinese herbal medicine,in the induction of remission in patients with mild-to-moderate ulcerative proctitis(UP).METHODS:We performed a multicenter,prospective,randomized,parallel-controlled trial to evaluate the efficacy of QCS induction therapy in 140 adult patients with mild-to-moderate UP and TCM syndrome of dampness-heat in large intestine.The patients were randomized to receive QCS(study group)or Salicylazosulfapyridine(SASP)suppository(control group)one piece each time,twice a day,per anum for 12 weeks.Mayo score and main symptoms score were evaluated at weeks 0,2,4,8 and 12,rectosigmoidscopy was taken at weeks 0,4,8 and 12,Geboes score,erythrocyte sedimentation rate(ESR),C-reactive protein(CRP)and safety indexes were assessed at weeks 0 and 12.The primary efficacy endpoint is clinical remission rate,the secondary efficacy endpoints are clinical response rate,mucosa healing rate,Geboes score,the remission rates of the main symptoms,the median day to the remission of the symptom,etc.RESULTS:There were no statistical difference in the clinical remission rates,the clinical response rates,the mucosa healing rates,Geboes score,ESR and CRP between the two groups.The remission rates of tenesmus and anal burning sensation of the study group were significantly higher than those of the control group(76.5%vs 25.0%,P=0.009;74.51%vs 29.63%,P=0.003).The median day to the remission of purulent bloody stool of the study group was significantly less than that of control group[11(1,64)vs 19(2,67),P=0.007].The patients receiving QCS had a significantly higher mucosa healing rate at week 4 than the patients receiving SASP suppository(71.42%vs 52.85%,P=0.023).No adverse event occurred in the study group while the adverse events incidence of the control group was 5.7%(P=0.049).CONCLUSIONS:QCS could induce the remission of UP as effectively and safely as SASP suppository,and was superior to SASP suppository in relieving the symptoms of tenesmus,anal burning sensation and purulent bloody stool and the time to reach mucosa healing.
文摘目的:评估超声内镜引导下细针穿刺(endoscopic ultrasound-guided fine needle aspiration,EUS-FNA)在胆管病变中的诊断效能和安全性,并探讨影响其准确性的因素。方法:回顾性分析2021年1月到2023年12月成都市第三人民医院因疑似胆管恶性狭窄/占位而接受EUS-FNA的41例患者临床资料,其中男性22例,女性19例,平均年龄为65.9(35.0~89.0)岁。评估其诊断效能(敏感性、特异性、阳性预测值、阴性预测值和准确性)及影响因素。结果:EUS-FNA对于胆管病变的总体灵敏性为85%,特异性为100%,阳性预测值为100%,阴性预测值为33%,准确性为86%;25G穿刺针和是否存在实性占位是影响EUS-FNA诊断胆管病变准确性的重要因素,而穿刺部位并未对诊断效能产生影响,参与分析的41例患者在随访期间未发现与EUS-FNA相关不良事件。结论:EUS-FNA在诊断胆管病变时具有较高的准确性和安全性,特别是使用25G穿刺针及存在胆管实性占位时,EUSFNA的诊断准确性更高。
文摘系统梳理中医药干预溃疡性结肠炎(UC)的随机对照试验(RCT),分析中医药临床试验的研究特征和结局指标,以期为后续开展中医药干预UC的RCT设计提供参考,为中医药在UC中的临床应用提供证据支持。计算机检索中国知网(CNKI)、万方(Wanfang)、维普(VIP)、中国生物医学文献服务系统(SinoMed)、PubMed、Cochrane Library、EMbase、Web of Science数据库2021年1月至2024年8月中医药治疗UC的RCT,评估偏倚风险,定性分析结局指标。最终纳入555篇RCT,样本量44853例,样本量最大为218例,最小为28例,多集中在60~100例;386篇RCT明确报告了中医证型,排序前3的分别是大肠湿热证(31.05%)、脾肾阳虚证(12.47%)及脾虚湿蕴证(9.17%);干预措施使用频数由高到低包括内服中药复方/制剂(64.5%)、中药复方/制剂保留灌肠(18.2%)、内服中药复方/制剂+中医外治(5.95%)、中医外治(4.86%)等;治疗疗程多集中在4~8周(64.86%),61篇(10.99%)研究报告了随访时间;共使用了157种结局指标,使用频数3460次,分属6个指标域:中医证候/症状(346次,10%)、症状/体征(541次,15.64%)、理化检查(2119次,61.24%)、生活质量(107次,3.09%)、远期预后(61次,1.76%)、安全性事件(284次,8.21%)。结果表明,中医药治疗UC的结局指标面临样本量计算缺乏依据、中医辨证分型欠规范、试验方案的设计与注册缺失、盲法与分配隐藏不足、干预措施的依从性、替代指标与终点指标选择不平衡、结局指标测量时点不统一以及设计待规范、伦理与安全性问题的局限性。建议未来应积极构建一套涵盖中医证型的标准化分类、明确的疗效评价指标、关键的终点指标及合理的测量时点,同时强调对远期预后影响的追踪、患者生活质量的全面评估及经济学效益考量的UC核心指标集,为中医药治疗UC的临床实践提供依据。
基金supported by Yunnan Provincial Basic Research Project(202401AT070344,202301AT070443)National Natural Science Foundation of China(62263014,52207105)+1 种基金Yunnan Lancang-Mekong International Electric Power Technology Joint Laboratory(202203AP140001)Major Science and Technology Projects in Yunnan Province(202402AG050006).
文摘Accurate short-term wind power forecast technique plays a crucial role in maintaining the safety and economic efficiency of smart grids.Although numerous studies have employed various methods to forecast wind power,there remains a research gap in leveraging swarm intelligence algorithms to optimize the hyperparameters of the Transformer model for wind power prediction.To improve the accuracy of short-term wind power forecast,this paper proposes a hybrid short-term wind power forecast approach named STL-IAOA-iTransformer,which is based on seasonal and trend decomposition using LOESS(STL)and iTransformer model optimized by improved arithmetic optimization algorithm(IAOA).First,to fully extract the power data features,STL is used to decompose the original data into components with less redundant information.The extracted components as well as the weather data are then input into iTransformer for short-term wind power forecast.The final predicted short-term wind power curve is obtained by combining the predicted components.To improve the model accuracy,IAOA is employed to optimize the hyperparameters of iTransformer.The proposed approach is validated using real-generation data from different seasons and different power stations inNorthwest China,and ablation experiments have been conducted.Furthermore,to validate the superiority of the proposed approach under different wind characteristics,real power generation data fromsouthwestChina are utilized for experiments.Thecomparative results with the other six state-of-the-art prediction models in experiments show that the proposed model well fits the true value of generation series and achieves high prediction accuracy.
基金supported by the CNPC Science and Technology Major Project of the Fourteenth Five-Year Plan(2021DJ0101)the National Natural Science Foundation of China(U19B600302,41872148)。
文摘The Early Cambrian Yuertusi Formation(Є_(1)y)in the Tarim Basin of China deposits a continuously developed suite of organic-rich black mudstones,which constitute an important source of oil and gas reservoirs in the Paleozoic.However,its hydrocarbon generation and evolution characteristics and resource potential have long been constrained by deeply buried strata and previous research.In this paper,based on the newly obtained ultra-deep well drilling data,the hydrocarbon generation and expulsion model ofЄ_(1)y shale was established by using data-driven Monte Carlo simulation,upon which the hydrocarbon generation,expulsion,and retention amounts were calculated by using the diagenetic method.The research indicates that theЄ_(1)y shale reaches the hydrocarbon generation and expulsion threshold at equivalent vitrinite reflectances of 0.46%and 0.72%,respectively.The cumulative hydrocarbon generation is 68.88×10^(10)t,the cumulative hydrocarbon expulsion is 35.59×10^(10)t,and the cumulative residual hydrocarbon is 33.29×10^(10)t.This paper systematically and quantitatively calculates the hydrocarbon expulsion at various key geological periods for theЄ_(1)y source rocks in the study area for the first time,more precisely confirming that the black shale of theЄ_(1)y is the most significant source rock contributing to the marine oil and gas resources in the Tarim Basin,filling the gap in hydrocarbon expulsion calculation in the study area,and providing an important basis for the formation and distribution of Paleozoic hydrocarbon reservoirs.The prospect of deep ultra-deep oil and gas exploration in the Tarim Basin is promising.Especially,the large area of dolomite reservoirs under the Cambrian salt and source rock interiors are the key breakthrough targets for the next exploration in the Tarim Basin.
