Objectives To reveal etiologies of persistent isolated hematuria （PIH） through ultrastructural pathological examination, to disclose clinicopathological correlation in cases with PIH, and to summarize appropriate ma...Objectives To reveal etiologies of persistent isolated hematuria （PIH） through ultrastructural pathological examination, to disclose clinicopathological correlation in cases with PIH, and to summarize appropriate management of patients with PIH. Methods we retrospectively studied 155 P1H patients receiving renal biopsy between January, 2003 and December, 2008 in Peking Union Medical College Hospital. All the clinical data and follow-up result were analyzed. Results All subjects included 38 children and 117 adults, with mean age of 11.38±3.25 years for children and 35.17±8.44 years for adults. Thin basement membrane nephropathy （TBMN） was the most common pathology （55.3% of children and 49.6% of adults）, followed by IgA nephropathy （18.4% of children and 32.5% of adults, mainly grade 2-3） and mesangial proliferative glomerulonephritis （MsPGN） without IgA deposition （13.2% of children and 12.8% of adults）. Besides, A1port syndrome （2.6% of children） and membrane nephropathy （2.6% of children and 0.9% of adults） were demonstrated as other causes of PIH. Elevated mean arteral pressure or protein excretion rate, as well as episodic macrohematuria, indicated higher risk for MsPGN rather than TBMN. On the other hand, severity of microhematuria was irrelevant to pathological types of PIH. Totally, 86 patients were followed up and 37 cases therein stayed on track for long term （mean duration 41.11±28.92 months, range 8-113 months）. Most cases had benign clinical course except 3 cases with TBMN, 5 cases with IgA nephropathy, 1 case with MsPGN （without IgA deposition）, and 1 case with Alport syndrome, who developed hypertension or proteinuria. All of them were administered timely intervention. Conclusions Close follow-up should be required as the primary management for PIH. Equally important is careful monitoring for early identification of undesirable predictors; while renal biopsy and other timely intervention are warranted if there is hypertension, significant proteinuria or renal impairment.展开更多
Polybrominated biphenyl ethers(PBDEs)and polycyclic aromatic hydrocarbons(PAHs)are commonly detected contaminants at e-waste recycling sites.Against the conventional wisdom that PBDEs and PAHs are highly immobile and ...Polybrominated biphenyl ethers(PBDEs)and polycyclic aromatic hydrocarbons(PAHs)are commonly detected contaminants at e-waste recycling sites.Against the conventional wisdom that PBDEs and PAHs are highly immobile and persist primarily in shallowsurface soils,increasing evidence shows that these compounds can leach into the groundwater.Herein,we compare the leachabilities of PBDEs vs.PAHs from contaminated soils collected at an e-waste recycling site in Tianjin,China.Considerable amounts of BDE-209(0.3–2 ng/L)and phenanthrene(42–106 ng/L),the most abundant PBDE and PAH at the site,are detected in the effluents of columns packed with contaminated soils,with the specific concentrations varying with hydrodynamic and solution chemistry conditions.Interestingly,the leaching potential of BDE-209 appears to be closely related to the release of colloidal mineral particles,whereas the leachability of phenanthrene correlates well with the concentration of dissolved organic carbon in the effluent,but showing essentially no correlation with the concentration of mineral particles.The surprisingly different trends of the leachability observed between BDE-209 and phenanthrene is counterintuitive,as PBDEs and PAHs often co-exist at e-waste recycling sites(particularly at the sites wherein incineration is being practiced)and share many similarities in terms of physicochemical properties.One possible explanation is that due to its extremely low solubility,BDE-209 predominantly exists in free-phase(i.e.,as solid(nano)particles),whereas the more soluble phenanthrene is mainly sorbed to soil organic matter.Findings in this study underscore the need to better understand the mobility of highly hydrophobic organic contaminants at contaminated sites for improved risk management.展开更多
Polybromodiphenyl ethers(PBDEs),the widely used flame retardants,are common contaminants in surface soils at e-waste recycling sites.The association of PBDEs with soil colloids has been observed,indicating the potenti...Polybromodiphenyl ethers(PBDEs),the widely used flame retardants,are common contaminants in surface soils at e-waste recycling sites.The association of PBDEs with soil colloids has been observed,indicating the potential risk to groundwater due to colloid-facilitated transport.However,the extent to which soil colloidsmay enhance the spreading of PBDEs in groundwater is largely unknown.Herein,we report the co-transport of decabromodiphenyl ester(BDE-209)and soil colloids in saturated porous media.The colloids released froma soil sample collected at an e-waste recycling site in Tianjin,China,contain high concentration of PBDEs,with BDE-209 being the most abundant conger(320±30 mg/kg).The colloids exhibit relatively high mobility in saturated sand columns,under conditions commonly observed in groundwater environments.Notably,under all the tested conditions(i.e.,varying flow velocity,pH,ionic species and ionic strength),the mass of eluted BDE-209 correlates linearly with that of eluted soil colloids,even though the mobility of the colloids varies markedly depending on the specific hydrodynamic and solution chemistry conditions involved.Additionally,the mass of BDE-209 retained in the columns also correlates strongly with themass of retained colloids.Apparently,the PBDEs remain bound to soil colloids during transport in porous media.Findings in this study indicate that soil colloidsmay significantly promote the transport of PBDEs in groundwater by serving as an effective carrier.This might be the reason why the highly insoluble and adsorptive PBDEs are found in groundwater at some PBDE-contaminated sites.展开更多
Modeling the fate and transport of organic pollutants at contaminated sites is critical for risk assessment and management practices,such as establishing realistic cleanup standards or remediation endpoints.Against th...Modeling the fate and transport of organic pollutants at contaminated sites is critical for risk assessment and management practices,such as establishing realistic cleanup standards or remediation endpoints.Against the conventional wisdom that highly hydrophobic persistent organic pollutants(POPs)(e.g.,polybrominated diphenyl ethers and polycyclic aromatic hydrocarbons)in surface soils are essentially immobile,mounting evidence has demonstrated the potential of these contaminants leaching into the groundwater,due to enhanced transport by soil colloids.Here,we develop a Colloids-Enhanced Transport(CET)model,which can be used as a simple screening tool to predict the leaching potential of POPs into groundwater,as mediated by soil colloids.The CET model incorporates several processes,including the release of POPs-bearing colloids into the porewater,the vertical transport of colloids and associated POPs in the vadose zone,themixing of POPs-containing soil leachate with groundwater,and the migration of POPs-bearing colloids in saturated zone.Thus,using parameters that can be easily obtained(e.g.,annual rainfall,soil type,and common hydrogeological properties of the subsurface porous media),the CET model can estimate the concentrations of POPs in the saturated zone from the observed POPs concentrations in surface or shallow subsurface zones.The CET model can also be used to derive soil quality standards or cleanup endpoints by back-calculating soil concentrations based on groundwater protection limits.展开更多
A new compound, （S）-2, 4-dihydroxy-1-butyl （4-hydroxy） benzoate （1）, and a known compound, fructigenines A （2）, were isolated from fungus Penicillium auratiogriseum derived from sponge Mycale plumose, by bioas...A new compound, （S）-2, 4-dihydroxy-1-butyl （4-hydroxy） benzoate （1）, and a known compound, fructigenines A （2）, were isolated from fungus Penicillium auratiogriseum derived from sponge Mycale plumose, by bioassay-guided fractionation. Their structures were established by spectroscopic and chemical methods. Both compounds show.ed cytotoxic activity against tsFT210 cells.展开更多
Nanostructured manganese oxides (nano-MnO_(x)) have shown great promises as versatile agrochemicals in nano-enabled sustainable agriculture,owing to the coupled benefits of controlled release of dissolved Mn2+,an esse...Nanostructured manganese oxides (nano-MnO_(x)) have shown great promises as versatile agrochemicals in nano-enabled sustainable agriculture,owing to the coupled benefits of controlled release of dissolved Mn2+,an essential nutrient needed by plants,and oxidative destruction of environmental organic pollutants.Here,we show that three δ-MnO_(2)nanomaterials consisting of nanosheet-assembled flower-like nanospheres not only exhibit greater kinetics in citrate-promoted dissolution,but also are less prone to passivation,compared with three α-MnO_(2)nanowire materials.The better performance of the δ-MnO_(2)nanomaterials can be attributed to their higher abundance of surface unsaturated Mn atoms–particularly Mn(Ⅲ)–that is originated from their specific exposed facets and higher abundance of surface defects sites.Our results underline the great potential of modulating nanomaterial surface atomic configuration to improve their performance in sustainable agricultural applications.展开更多
Objective:To investigate the effect of combined detection of serum carcinoembryonic antigen(CEA),cytokeratin 19 fragment(CYFRA21-1),cancer antigen 125(CA125),and neuron-specific enolase(NSE)in patients with lung cance...Objective:To investigate the effect of combined detection of serum carcinoembryonic antigen(CEA),cytokeratin 19 fragment(CYFRA21-1),cancer antigen 125(CA125),and neuron-specific enolase(NSE)in patients with lung cancer by fluorescence flow cytometry.Methods:From August 2019 to July 2022,200 patients with lung cancer diagnosed by pathology in our hospital were retrospectively analyzed.2 mL venous blood was collected in a fasting state and centrifuged to separate the serum(containing human chorionic gonadotropin antibody[anti-hCG antibody],hepatitis B surface antibody[anti-HBs antibody],and CEA).Results:The sensitivities of CEA and CYFRA21-1 detected via enzyme-linked immunosorbent assay(ELISA)were 100%,and the detection limits were 0.5 ng/mL and 0.1 ng/mL,respectively;the sensitivities of CA125 and NSE detected via flow cytometry were 100%,and the detection limits were 10 U/mL and 2 ng/mL,respectively.Compared with ELISA,the sensitivities of CA125 and NSE detected via flow cytometry were higher.When the concentration of CEA was 10-40 ng/mL,the sensitivities of the three markers CYFRA21-1,CA125,and NSE showed no significant changes(P>0.05);when the concentration of CEA was 40-80 ng/mL,the sensitivity of CEA significantly decreased(P<0.01),but the sensitivities of the three markers CYFRA21-1,CA125,and NSE showed no significant changes(P>0.05);when the concentration of CEA was 80-200 ng/mL,the sensitivities of all four markers showed no significant changes(P>0.05).Conclusion:Compared with the double-antibody sandwich ELISA,fluorescence flow cytometry has certain advantages,including high sensitivity,good precision,short detection time,low sample usage,and low medical cost;thus,it is worthy of clinical promotion.展开更多
Objective:To investigate the expression and impact of helper T cell type 17 and CD4^(+)CD25^(+)regulatory T(Treg)cells in anti-mitochondrial M2 antibody(AMA-M2)positive primary biliary cholangitis(PBC)patients.Methods...Objective:To investigate the expression and impact of helper T cell type 17 and CD4^(+)CD25^(+)regulatory T(Treg)cells in anti-mitochondrial M2 antibody(AMA-M2)positive primary biliary cholangitis(PBC)patients.Methods:Thirty PBC patients with positive AMA(M2 type)(antibody titer above 1:320)by indirect immunofluorescence assay under the Affiliated Hospital of Hebei University from November 2021 to August 2022 were selected as the experimental group,while 30 healthy individuals were selected as controls.The subjects were observed and analyzed for AFP-L3 and immunoglobulin expression.Results:The levels of Th17,Treg,Th17/Treg,interleukin(IL)-17A,IL-2,IL-10,and transforming growth factor(TGF)-β1 cytokines of the experimental group were 2.61±0.48,1.15±0.54,2.41±0.47,310.94±21.14,276.36±36.12,317.89±28.97,and 197.48±31.04,respectively,while those of the control group were 1.14±0.58,0.88±0.29,1.47±0.25,9.69±1.26,57.69±2.45,154.01±19.87,and 514.36±36.12,respectively,wherein P<0.05;the CD4^(+),CD8^(+),and CD4^(+)/CD8^(+)of the experimental group were 39.48±4.19,20.12±4.41,and 1.76±0.14,respectively,while those of the control group were 35.78±4.21,22.01±4.16,and 1.51±0.13,respectively,wherein P<0.05.Conclusion:In patients with PBC,there is a significant imbalance in Th17/Treg cells.Il-17A,IL-2,IL-10,and TGF-β1 cytokines play important roles in the differentiation and functional expression of both Th17 and Treg cells.展开更多
Polybrominated diphenyl ethers(PBDEs)are ubiquitous contaminants,especially in the soil and groundwater of contaminated sites and landfills.Notably,2,20,3,30,4,40,5,50,6,60-decabromodiphenyl ether(BDE-209),one of the ...Polybrominated diphenyl ethers(PBDEs)are ubiquitous contaminants,especially in the soil and groundwater of contaminated sites and landfills.Notably,2,20,3,30,4,40,5,50,6,60-decabromodiphenyl ether(BDE-209),one of the most frequently and abundantly detected PBDE congeners in the environment,has recently been designated as a new pollutant subject to rigorous control in China.Colloid-facilitated transport is a key mechanism for the release of PBDEs from surface soils and their migration in the aquifer,but the effects of hydrodynamic conditions,particularly transient flow,on colloid-facilitated release of PBDEs are not well understood.Herein,we examined the effects of typical transient flow conditions on the release characteristics of colloids and BDE-209 from surface soil collected from an e-waste recycling site by undisturbed soil core leaching tests involving multiple dry–wet cycles(with different drying durations)and freeze–thaw cycles.We observed significant positive correlations between BDE-209 and colloid concentrations in the leachate in both the dry–wet and freeze–thaw leaching experiments,highlighting the critical role of colloids in facilitating BDE-209 release.However,colloids mobilized during the dry–wet cycles contained higher contents of BDE-209 than those in the freeze–thaw cycle tests,and the difference was primarily due to the more intensive disintegration of soil aggregates and elution of newly formed inorganic colloidal particles(mainly primary silicate minerals such as quartz and albite)with low BDE-209 content during the freeze–thaw process.These findings underscore the necessity of considering transient flow conditions when assessing the fate and risks of PBDEs at contaminated sites.展开更多
Dealing with groundwater impacted by persistent,low-concentration chlorinated solvents is a major challenge for site remediation,as technologies designed for fast contaminant removal or destruction often are not cost-...Dealing with groundwater impacted by persistent,low-concentration chlorinated solvents is a major challenge for site remediation,as technologies designed for fast contaminant removal or destruction often are not cost-effective.For long-term plume management,in situ contaminant sequestration using carbonaceous materials is a more viable strategy.Here,we prove the concept that the effectiveness of this approach can be improved by modulating the compositional and surface properties of carbonaceous materials to maximize the synergy between contaminant binding and abiotic transformation.We found that two pine wood biochars pyrolyzed at 600 and 700℃exhibit not only faster adsorption kinetics for 1,1,2,2-tetrachloroethane than those prepared at lower temperatures(500℃and below),but also greater efficacy in enhancing the dehydrochlorination of the contaminant.The higher catalytic efficiency is counterintuitive,as it is commonly accepted that surface carboxyl and phenolic groups are the catalytic sites.With supplementary experiments carried out using modified materials and at varied pH values,we found that the surprisingly higher catalytic activities of these two samples are due to their higher carbonate contents.Interestingly,trichloroethylene,the hydrolysis product,is more adsorptive to the biochars than the parent compound.Thus,by promoting the abiotic transformation,these two biochars enable much more effective plume interception than the less-reactive materials.The findings have important implications for dealing with long-term,persistent groundwater contamination,particularly,the“rebounding”problem often occurring post active site remediation.展开更多
文摘Objectives To reveal etiologies of persistent isolated hematuria （PIH） through ultrastructural pathological examination, to disclose clinicopathological correlation in cases with PIH, and to summarize appropriate management of patients with PIH. Methods we retrospectively studied 155 P1H patients receiving renal biopsy between January, 2003 and December, 2008 in Peking Union Medical College Hospital. All the clinical data and follow-up result were analyzed. Results All subjects included 38 children and 117 adults, with mean age of 11.38±3.25 years for children and 35.17±8.44 years for adults. Thin basement membrane nephropathy （TBMN） was the most common pathology （55.3% of children and 49.6% of adults）, followed by IgA nephropathy （18.4% of children and 32.5% of adults, mainly grade 2-3） and mesangial proliferative glomerulonephritis （MsPGN） without IgA deposition （13.2% of children and 12.8% of adults）. Besides, A1port syndrome （2.6% of children） and membrane nephropathy （2.6% of children and 0.9% of adults） were demonstrated as other causes of PIH. Elevated mean arteral pressure or protein excretion rate, as well as episodic macrohematuria, indicated higher risk for MsPGN rather than TBMN. On the other hand, severity of microhematuria was irrelevant to pathological types of PIH. Totally, 86 patients were followed up and 37 cases therein stayed on track for long term （mean duration 41.11±28.92 months, range 8-113 months）. Most cases had benign clinical course except 3 cases with TBMN, 5 cases with IgA nephropathy, 1 case with MsPGN （without IgA deposition）, and 1 case with Alport syndrome, who developed hypertension or proteinuria. All of them were administered timely intervention. Conclusions Close follow-up should be required as the primary management for PIH. Equally important is careful monitoring for early identification of undesirable predictors; while renal biopsy and other timely intervention are warranted if there is hypertension, significant proteinuria or renal impairment.
基金supported by the National Key Research and Development Program of China(No.2019YFC1804202)the National Natural Science Foundation of China(Nos.22020102004 and 22125603)+1 种基金Tianjin Municipal Science and Technology Bureau(No.21JCZDJC00280)the Fundamental Research Funds for the Central Universities,and the Ministry of Education of China(No.T2017002).
文摘Polybrominated biphenyl ethers(PBDEs)and polycyclic aromatic hydrocarbons(PAHs)are commonly detected contaminants at e-waste recycling sites.Against the conventional wisdom that PBDEs and PAHs are highly immobile and persist primarily in shallowsurface soils,increasing evidence shows that these compounds can leach into the groundwater.Herein,we compare the leachabilities of PBDEs vs.PAHs from contaminated soils collected at an e-waste recycling site in Tianjin,China.Considerable amounts of BDE-209(0.3–2 ng/L)and phenanthrene(42–106 ng/L),the most abundant PBDE and PAH at the site,are detected in the effluents of columns packed with contaminated soils,with the specific concentrations varying with hydrodynamic and solution chemistry conditions.Interestingly,the leaching potential of BDE-209 appears to be closely related to the release of colloidal mineral particles,whereas the leachability of phenanthrene correlates well with the concentration of dissolved organic carbon in the effluent,but showing essentially no correlation with the concentration of mineral particles.The surprisingly different trends of the leachability observed between BDE-209 and phenanthrene is counterintuitive,as PBDEs and PAHs often co-exist at e-waste recycling sites(particularly at the sites wherein incineration is being practiced)and share many similarities in terms of physicochemical properties.One possible explanation is that due to its extremely low solubility,BDE-209 predominantly exists in free-phase(i.e.,as solid(nano)particles),whereas the more soluble phenanthrene is mainly sorbed to soil organic matter.Findings in this study underscore the need to better understand the mobility of highly hydrophobic organic contaminants at contaminated sites for improved risk management.
基金supported by the National Key Research and Development Program of China(No.2019YFC1804202)the National Natural Science Foundation of China(No.22020102004)+1 种基金the Tianjin Municipal Science and Technology Bureau(No.21JCZDJC00280)the Fundamental Research Funds for the Central Universities by the Ministry of Education of China(No.T2017002).
文摘Polybromodiphenyl ethers(PBDEs),the widely used flame retardants,are common contaminants in surface soils at e-waste recycling sites.The association of PBDEs with soil colloids has been observed,indicating the potential risk to groundwater due to colloid-facilitated transport.However,the extent to which soil colloidsmay enhance the spreading of PBDEs in groundwater is largely unknown.Herein,we report the co-transport of decabromodiphenyl ester(BDE-209)and soil colloids in saturated porous media.The colloids released froma soil sample collected at an e-waste recycling site in Tianjin,China,contain high concentration of PBDEs,with BDE-209 being the most abundant conger(320±30 mg/kg).The colloids exhibit relatively high mobility in saturated sand columns,under conditions commonly observed in groundwater environments.Notably,under all the tested conditions(i.e.,varying flow velocity,pH,ionic species and ionic strength),the mass of eluted BDE-209 correlates linearly with that of eluted soil colloids,even though the mobility of the colloids varies markedly depending on the specific hydrodynamic and solution chemistry conditions involved.Additionally,the mass of BDE-209 retained in the columns also correlates strongly with themass of retained colloids.Apparently,the PBDEs remain bound to soil colloids during transport in porous media.Findings in this study indicate that soil colloidsmay significantly promote the transport of PBDEs in groundwater by serving as an effective carrier.This might be the reason why the highly insoluble and adsorptive PBDEs are found in groundwater at some PBDE-contaminated sites.
基金supported by the National Key Research and Development Program of China(No.2019YFC1804202)the National Natural Science Foundation of China(Nos.22020102004 and 22125603)+1 种基金Tianjin Municipal Science and Technology Bureau(No.21JCZDJC00280)the Fundamental Research Funds for the Central Universities,and the Ministry of Education of China(No.T2017002).
文摘Modeling the fate and transport of organic pollutants at contaminated sites is critical for risk assessment and management practices,such as establishing realistic cleanup standards or remediation endpoints.Against the conventional wisdom that highly hydrophobic persistent organic pollutants(POPs)(e.g.,polybrominated diphenyl ethers and polycyclic aromatic hydrocarbons)in surface soils are essentially immobile,mounting evidence has demonstrated the potential of these contaminants leaching into the groundwater,due to enhanced transport by soil colloids.Here,we develop a Colloids-Enhanced Transport(CET)model,which can be used as a simple screening tool to predict the leaching potential of POPs into groundwater,as mediated by soil colloids.The CET model incorporates several processes,including the release of POPs-bearing colloids into the porewater,the vertical transport of colloids and associated POPs in the vadose zone,themixing of POPs-containing soil leachate with groundwater,and the migration of POPs-bearing colloids in saturated zone.Thus,using parameters that can be easily obtained(e.g.,annual rainfall,soil type,and common hydrogeological properties of the subsurface porous media),the CET model can estimate the concentrations of POPs in the saturated zone from the observed POPs concentrations in surface or shallow subsurface zones.The CET model can also be used to derive soil quality standards or cleanup endpoints by back-calculating soil concentrations based on groundwater protection limits.
文摘A new compound, （S）-2, 4-dihydroxy-1-butyl （4-hydroxy） benzoate （1）, and a known compound, fructigenines A （2）, were isolated from fungus Penicillium auratiogriseum derived from sponge Mycale plumose, by bioassay-guided fractionation. Their structures were established by spectroscopic and chemical methods. Both compounds show.ed cytotoxic activity against tsFT210 cells.
基金supported by the National Key Research and Development Program of China (Nos. 2018YFC1800705 and 2019YFC1804202)the Fundamental Research Funds for the Central Universities (Nankai University 63211078)+1 种基金the Ministry of Education of China (No. T2017002)Tianjin Municipal Science and Technology Bureau (No. 20JCQNJC02050)。
文摘Nanostructured manganese oxides (nano-MnO_(x)) have shown great promises as versatile agrochemicals in nano-enabled sustainable agriculture,owing to the coupled benefits of controlled release of dissolved Mn2+,an essential nutrient needed by plants,and oxidative destruction of environmental organic pollutants.Here,we show that three δ-MnO_(2)nanomaterials consisting of nanosheet-assembled flower-like nanospheres not only exhibit greater kinetics in citrate-promoted dissolution,but also are less prone to passivation,compared with three α-MnO_(2)nanowire materials.The better performance of the δ-MnO_(2)nanomaterials can be attributed to their higher abundance of surface unsaturated Mn atoms–particularly Mn(Ⅲ)–that is originated from their specific exposed facets and higher abundance of surface defects sites.Our results underline the great potential of modulating nanomaterial surface atomic configuration to improve their performance in sustainable agricultural applications.
文摘Objective:To investigate the effect of combined detection of serum carcinoembryonic antigen(CEA),cytokeratin 19 fragment(CYFRA21-1),cancer antigen 125(CA125),and neuron-specific enolase(NSE)in patients with lung cancer by fluorescence flow cytometry.Methods:From August 2019 to July 2022,200 patients with lung cancer diagnosed by pathology in our hospital were retrospectively analyzed.2 mL venous blood was collected in a fasting state and centrifuged to separate the serum(containing human chorionic gonadotropin antibody[anti-hCG antibody],hepatitis B surface antibody[anti-HBs antibody],and CEA).Results:The sensitivities of CEA and CYFRA21-1 detected via enzyme-linked immunosorbent assay(ELISA)were 100%,and the detection limits were 0.5 ng/mL and 0.1 ng/mL,respectively;the sensitivities of CA125 and NSE detected via flow cytometry were 100%,and the detection limits were 10 U/mL and 2 ng/mL,respectively.Compared with ELISA,the sensitivities of CA125 and NSE detected via flow cytometry were higher.When the concentration of CEA was 10-40 ng/mL,the sensitivities of the three markers CYFRA21-1,CA125,and NSE showed no significant changes(P>0.05);when the concentration of CEA was 40-80 ng/mL,the sensitivity of CEA significantly decreased(P<0.01),but the sensitivities of the three markers CYFRA21-1,CA125,and NSE showed no significant changes(P>0.05);when the concentration of CEA was 80-200 ng/mL,the sensitivities of all four markers showed no significant changes(P>0.05).Conclusion:Compared with the double-antibody sandwich ELISA,fluorescence flow cytometry has certain advantages,including high sensitivity,good precision,short detection time,low sample usage,and low medical cost;thus,it is worthy of clinical promotion.
基金supported by Baoding Science and Technology Planning Project(Grant Number:2141ZF316).
文摘Objective:To investigate the expression and impact of helper T cell type 17 and CD4^(+)CD25^(+)regulatory T(Treg)cells in anti-mitochondrial M2 antibody(AMA-M2)positive primary biliary cholangitis(PBC)patients.Methods:Thirty PBC patients with positive AMA(M2 type)(antibody titer above 1:320)by indirect immunofluorescence assay under the Affiliated Hospital of Hebei University from November 2021 to August 2022 were selected as the experimental group,while 30 healthy individuals were selected as controls.The subjects were observed and analyzed for AFP-L3 and immunoglobulin expression.Results:The levels of Th17,Treg,Th17/Treg,interleukin(IL)-17A,IL-2,IL-10,and transforming growth factor(TGF)-β1 cytokines of the experimental group were 2.61±0.48,1.15±0.54,2.41±0.47,310.94±21.14,276.36±36.12,317.89±28.97,and 197.48±31.04,respectively,while those of the control group were 1.14±0.58,0.88±0.29,1.47±0.25,9.69±1.26,57.69±2.45,154.01±19.87,and 514.36±36.12,respectively,wherein P<0.05;the CD4^(+),CD8^(+),and CD4^(+)/CD8^(+)of the experimental group were 39.48±4.19,20.12±4.41,and 1.76±0.14,respectively,while those of the control group were 35.78±4.21,22.01±4.16,and 1.51±0.13,respectively,wherein P<0.05.Conclusion:In patients with PBC,there is a significant imbalance in Th17/Treg cells.Il-17A,IL-2,IL-10,and TGF-β1 cytokines play important roles in the differentiation and functional expression of both Th17 and Treg cells.
基金supported by the National Key Research and Development Program of China(2019YFC1804202)the National Natural Science Foundation of China(22276101 and 22020102004)+1 种基金the Fundamental Research Funds for the Central Universities(63233056)the Ministry of Education of China(T2017002).
文摘Polybrominated diphenyl ethers(PBDEs)are ubiquitous contaminants,especially in the soil and groundwater of contaminated sites and landfills.Notably,2,20,3,30,4,40,5,50,6,60-decabromodiphenyl ether(BDE-209),one of the most frequently and abundantly detected PBDE congeners in the environment,has recently been designated as a new pollutant subject to rigorous control in China.Colloid-facilitated transport is a key mechanism for the release of PBDEs from surface soils and their migration in the aquifer,but the effects of hydrodynamic conditions,particularly transient flow,on colloid-facilitated release of PBDEs are not well understood.Herein,we examined the effects of typical transient flow conditions on the release characteristics of colloids and BDE-209 from surface soil collected from an e-waste recycling site by undisturbed soil core leaching tests involving multiple dry–wet cycles(with different drying durations)and freeze–thaw cycles.We observed significant positive correlations between BDE-209 and colloid concentrations in the leachate in both the dry–wet and freeze–thaw leaching experiments,highlighting the critical role of colloids in facilitating BDE-209 release.However,colloids mobilized during the dry–wet cycles contained higher contents of BDE-209 than those in the freeze–thaw cycle tests,and the difference was primarily due to the more intensive disintegration of soil aggregates and elution of newly formed inorganic colloidal particles(mainly primary silicate minerals such as quartz and albite)with low BDE-209 content during the freeze–thaw process.These findings underscore the necessity of considering transient flow conditions when assessing the fate and risks of PBDEs at contaminated sites.
基金supported by the National Natural Science Foundation of China(22125603 and 22020102004)Tianjin Municipal Science and Technology Bureau(23 JCYBJC01650,23 JCQNJC01340 and 21 JCJQJC00060)the Fundamental Research Funds for the Central Universities and the Ministry of Education of China(T2017002).
文摘Dealing with groundwater impacted by persistent,low-concentration chlorinated solvents is a major challenge for site remediation,as technologies designed for fast contaminant removal or destruction often are not cost-effective.For long-term plume management,in situ contaminant sequestration using carbonaceous materials is a more viable strategy.Here,we prove the concept that the effectiveness of this approach can be improved by modulating the compositional and surface properties of carbonaceous materials to maximize the synergy between contaminant binding and abiotic transformation.We found that two pine wood biochars pyrolyzed at 600 and 700℃exhibit not only faster adsorption kinetics for 1,1,2,2-tetrachloroethane than those prepared at lower temperatures(500℃and below),but also greater efficacy in enhancing the dehydrochlorination of the contaminant.The higher catalytic efficiency is counterintuitive,as it is commonly accepted that surface carboxyl and phenolic groups are the catalytic sites.With supplementary experiments carried out using modified materials and at varied pH values,we found that the surprisingly higher catalytic activities of these two samples are due to their higher carbonate contents.Interestingly,trichloroethylene,the hydrolysis product,is more adsorptive to the biochars than the parent compound.Thus,by promoting the abiotic transformation,these two biochars enable much more effective plume interception than the less-reactive materials.The findings have important implications for dealing with long-term,persistent groundwater contamination,particularly,the“rebounding”problem often occurring post active site remediation.
