为初步解析马尾松花粉对超低温保存的生理响应及相关代谢机制,该文以马尾松花粉为研究对象,在-196℃液氮进行超低温保存并分析冷存过程中[冻存前(CK)、液氮冻存后(LD)、化冻后(HD)]与活性氧(ROS)相关的生理指标及转录组数据。结果表明:...为初步解析马尾松花粉对超低温保存的生理响应及相关代谢机制,该文以马尾松花粉为研究对象,在-196℃液氮进行超低温保存并分析冷存过程中[冻存前(CK)、液氮冻存后(LD)、化冻后(HD)]与活性氧(ROS)相关的生理指标及转录组数据。结果表明:(1)马尾松花粉超低温保存的最适含水量为3.96%,该含水量下将花粉直接投入液氮保存至少48 h,冻存前后花粉存活率分别为78.54%和73.80%。(2)超氧化物歧化酶(SOD),抗坏血酸过氧化物酶(GSH),谷胱甘肽(APX),抑制羟自由基能力4个指标在冻存前后差异显著。(3)转录组测序共获得65.60 Gb过滤数据,有38505个基因比对到参考基因组(47.84%),CK vs LD、CK vs HD、LD vs HD的差异表达基因(DEGs数量分别为232个、268个、218个)。GO和KEGG分析表明,应激响应(response to stimulus)和抗氧化活性(antioxidant activity)等GO term显著富集;植物激素信号转导、MAPK信号途径、淀粉和蔗糖代谢、果糖和甘露醇代谢、过氧化物酶体等KEGG途径显著富集,从这些途径中进一步筛选到10个可能与冷冻保存过程中损伤和修复有关的基因。该研究结果为马尾松种质资源高效利用和安全保存提供了技术手段,也为进一步解析冷冻保存损伤的分子机制提供参考。展开更多
The importance of selective synthesis of high-value-added chemicals from renewable resources is paramount but remains a crucial challenge in organic synthesis and chemical reformation.Herein,we report the selective ph...The importance of selective synthesis of high-value-added chemicals from renewable resources is paramount but remains a crucial challenge in organic synthesis and chemical reformation.Herein,we report the selective photosynthesis of C-C coupling products and carbonyl compounds from biomass-derived alcohols.The key to ensuring high end-to-end selectivity is the modulation of the reactivity of ketyl radical(*RCHOH)intermediates by employing different metal co-catalysts(Au,Pt,Pd,Ru)supported on Cd0.6Zn0.4S solid solution(CZS)photocatalysts.In particular,the C-C coupling product,hydrobenzion,and fully oxidized benzaldehyde were obtained from benzyl alcohol with high selectivity(>98%)over Au-CZS and Ru-CZS,respectively.Combined experimental and theoretical analyses demonstrated that the affinity of*RCHOH for the surface of metals governs their subsequent transformations,in which weak and strong radical adsorption on Au and Ru results in C-C coupling products and carbonyl compounds,respectively.展开更多
文摘为初步解析马尾松花粉对超低温保存的生理响应及相关代谢机制,该文以马尾松花粉为研究对象,在-196℃液氮进行超低温保存并分析冷存过程中[冻存前(CK)、液氮冻存后(LD)、化冻后(HD)]与活性氧(ROS)相关的生理指标及转录组数据。结果表明:(1)马尾松花粉超低温保存的最适含水量为3.96%,该含水量下将花粉直接投入液氮保存至少48 h,冻存前后花粉存活率分别为78.54%和73.80%。(2)超氧化物歧化酶(SOD),抗坏血酸过氧化物酶(GSH),谷胱甘肽(APX),抑制羟自由基能力4个指标在冻存前后差异显著。(3)转录组测序共获得65.60 Gb过滤数据,有38505个基因比对到参考基因组(47.84%),CK vs LD、CK vs HD、LD vs HD的差异表达基因(DEGs数量分别为232个、268个、218个)。GO和KEGG分析表明,应激响应(response to stimulus)和抗氧化活性(antioxidant activity)等GO term显著富集;植物激素信号转导、MAPK信号途径、淀粉和蔗糖代谢、果糖和甘露醇代谢、过氧化物酶体等KEGG途径显著富集,从这些途径中进一步筛选到10个可能与冷冻保存过程中损伤和修复有关的基因。该研究结果为马尾松种质资源高效利用和安全保存提供了技术手段,也为进一步解析冷冻保存损伤的分子机制提供参考。
文摘The importance of selective synthesis of high-value-added chemicals from renewable resources is paramount but remains a crucial challenge in organic synthesis and chemical reformation.Herein,we report the selective photosynthesis of C-C coupling products and carbonyl compounds from biomass-derived alcohols.The key to ensuring high end-to-end selectivity is the modulation of the reactivity of ketyl radical(*RCHOH)intermediates by employing different metal co-catalysts(Au,Pt,Pd,Ru)supported on Cd0.6Zn0.4S solid solution(CZS)photocatalysts.In particular,the C-C coupling product,hydrobenzion,and fully oxidized benzaldehyde were obtained from benzyl alcohol with high selectivity(>98%)over Au-CZS and Ru-CZS,respectively.Combined experimental and theoretical analyses demonstrated that the affinity of*RCHOH for the surface of metals governs their subsequent transformations,in which weak and strong radical adsorption on Au and Ru results in C-C coupling products and carbonyl compounds,respectively.
