Spermatogenesis, maturation, capacitation and fertilization are precisely regulated by glycosylation. However, the relationship between altered glycosylation patterns and the onset and development of reproductive diso...Spermatogenesis, maturation, capacitation and fertilization are precisely regulated by glycosylation. However, the relationship between altered glycosylation patterns and the onset and development of reproductive disorders is unclear, mainly limited by the lack of in situ imaging techniques for spermatozoa glycosylation. We developed an efficient and highly specific spermatozoa glycan imaging technique based on the robust chemoselective labeling of sialic acid(Sia) and N-acetyl-D-galactosamine(Gal/GalNAc). We further proposed a “tandem glycan chemoselective labeling” strategy to achieve simultaneous imaging of two types of glycans on spermatozoa. We applied the developed method to the spermatozoa from oligozoospermic patients and diabetic mice and found that these spermatozoa showed higher levels of Sia and Gal/Gal NAc expression than the normal groups. Moreover, spermatozoa from diabetic mice showed a severe decrease in number, viability, and forward motility, suggesting that in vivo glucose metabolism disorders may lead to an elevated level of spermatozoa glycosylation and have a correlation with the development of oligoasthenotspermia. Our work provides a research tool to reveal the relationship between glycosylation modification and spermatozoa quality, and a promising clue for the development of glycan-based reproductive markers.展开更多
To investigate groundwater flow and solute transport characteristics of the karst trough zone in China,tracer experiments were conducted at two adjacent typical karst groundwater flow systems(Yuquandong(YQD)and Migong...To investigate groundwater flow and solute transport characteristics of the karst trough zone in China,tracer experiments were conducted at two adjacent typical karst groundwater flow systems(Yuquandong(YQD)and Migongquan(MGQ))in Sixi valley,western Hubei,China.Highresolution continuous monitoring was utilized to obtain breakthrough curves(BTCs),which were then analyzed using the multi-dispersion model(MDM)and the two-region nonequilibrium model(2RNE)with basic parameters calculated by CXTFIT and QTRACER2.Results showed that:(1)YQD flow system had a complex infiltration matrix with overland flow,conduit flow and fracture flow,while the MGQ flow system was dominated by conduit flow with fast flow transport velocity,but also small amount of fracture flow there;(2)They were well fitted based on the MDM(R^2=0.928)and 2RNE(R^2=0.947)models,indicating that they had strong adaptability in the karst trough zone;(3)conceptual models for YQD and MGQ groundwater systems were generalized.In YQD system,the solute was transported via overland flow during intense rainfall,while some infiltrated down into fissures and conduits.In MGQ system,most were directly transported to spring outlet in the fissureconduit network.展开更多
The Zongwulong Shan-Qinghai Nanshan tectonic belt of the northern Xizang Plateau experienced a protracted tectonica history,including the openings and closures of the Proto-and Paleo-Tethyan Oceans.Although the tecton...The Zongwulong Shan-Qinghai Nanshan tectonic belt of the northern Xizang Plateau experienced a protracted tectonica history,including the openings and closures of the Proto-and Paleo-Tethyan Oceans.Although the tectonic belt has been extensively studied,details regarding the tectonic processes involved in its development remain controversial.To better constrain the tectonic processes of this tectonic belt,we conducted detailed field geological mapping,zircon U-Pb geochronology,and whole-rock geochemical and Sr-Nd isotopic analyses.Our results show that intrusive rocks in the tectonic belt crystallized in ca.292-233 Ma,perhaps in an arc/subduction setting.Geochemical and Sr-Nd isotopic data suggest that Early Permian-Late Triassic ultramaficintermediate intrusions were sourced from the enriched mantle,whereas intermediate-acidic rocks were sourced from mixed crust-mantle.We present the tectonic model that involves:(1)Early Devonian-Early Permian intracontinental extension occurred in the northern margin of the Qaidam continent(ca.416-292 Ma);(2)Early Permian-Late Triassic northward subduction of the Paleo-Tethyan Ocean resulted in arc magmatism(ca.292-233 Ma);and(3)subsequent Late Triassic intracontinental extension(ca.233-215 Ma).Our results suggest that the Late Paleozoic-Early Mesozoic development of the Zongwulong Shan-Qinghai Nanshan was related to the opening,subduction,and slab retreat of the Paleo-Tethyan Ocean,which has key implications for the tectonic evolution of the northern Tibetan Plateau.展开更多
Objective:This study aimed to investigate the prevalence,causes,and influencing factors of vision impairment in the elderly population aged 60 years and above in Mangxin Town,Kashgar region,Xinjiang,China.Located in a...Objective:This study aimed to investigate the prevalence,causes,and influencing factors of vision impairment in the elderly population aged 60 years and above in Mangxin Town,Kashgar region,Xinjiang,China.Located in a region characterized by intense ultraviolet radiation and arid climatic conditions,Mangxin Town presents unique environmental challenges that may exacerbate ocular health issues.Despite the global emphasis on addressing vision impairment among aging populations,there remains a paucity of updated and region-specific data in Xinjiang,necessitating this comprehensive assessment to inform targeted interventions.Methods:A cross-sectional study was conducted from May to June 2024,involving 1,311 elderly participants(76.76%participation rate)out of a total eligible population of 1,708 individuals aged≥60 years.Participants underwent detailed ocular examinations,including assessments of uncorrected visual acuity(UVA)and best-corrected visual acuity(BCVA)using standard logarithmic charts,slit-lamp biomicroscopy,optical coherence tomography(OCT,Topcon DRI OCT Triton),fundus photography,and intraocular pressure measurement(Canon TX-20 Tonometer).A multidisciplinary team of 10 ophthalmologists and 2 local village doctors,trained rigorously in standardized protocols,ensured consistent data collection.Demographic,lifestyle,and medical history data were collected via questionnaires.Statistical analyses,performed using STATA 16,included multivariate logistic regression to identify risk factors,with significance defined as P<0.05.Results:The overall prevalence of vision impairment was 13.21%(95%CI:11.37%-15.04%),with low vision at 11.76%(95%CI:10.01%-13.50%)and blindness at 1.45%(95%CI:0.80%-2.10%).Cataract emerged as the leading cause,responsible for 68.20%of cases,followed by glaucoma(5.80%),optic atrophy(5.20%),and age-related macular degeneration(2.90%).Vision impairment prevalence escalated significantly with age:7.74%in the 60–69 age group,17.79%in 70–79,and 33.72%in those≥80.Males exhibited higher prevalence than females(15.84%vs.10.45%,P=0.004).Multivariate analysis revealed age≥80 years(OR=6.43,95%CI:3.79%-10.90%),male sex(OR=0.53,95%CI:0.34%-0.83%),and daily exercise(OR=0.44,95%CI:0.20%-0.95%)as significant factors.History of eye disease showed a non-significant trend toward increased risk(OR=1.49,P=0.107).Education level,income,and smoking status showed no significant associations.Conclusions:This study underscores cataract as the predominant cause of vision impairment in Mangxin Town’s elderly population,with age and sex as critical determinants.The findings align with global patterns but highlight region-specific challenges,such as environmental factors contributing to cataract prevalence.Public health strategies should prioritize improving access to cataract surgery,enhancing grassroots ophthalmic infrastructure,and integrating portable screening technologies for early detection of fundus diseases.Additionally,promoting health education on UV protection and lifestyle modifications,such as regular exercise,may mitigate risks.Future research should expand to broader regions in Xinjiang,employ advanced diagnostic tools for complex conditions like glaucoma,and explore longitudinal trends to refine intervention strategies.These efforts are vital to reducing preventable blindness and improving quality of life for aging populations in underserved areas.展开更多
化学事故具有突发性强、环境污染破坏严重、救援难度大等特点,对突发化学事故的影响范围、破坏程度进行快速评估,为事故抢险救援提供决策支持,是应对重特大突发化学事故、降低事故损失的重要手段。本文基于三维地理信息系统(Three-dimen...化学事故具有突发性强、环境污染破坏严重、救援难度大等特点,对突发化学事故的影响范围、破坏程度进行快速评估,为事故抢险救援提供决策支持,是应对重特大突发化学事故、降低事故损失的重要手段。本文基于三维地理信息系统(Three-dimensional Geographic Information System,3D GIS),在充分考虑下垫面影响的前提下,研究了大气污染扩散模型,提出了扩散场快速构建技术及兼顾污染分区的救援力量调度方法,并以此为核心建立了系统总体框架,形成了集扩散模拟、危害分析、救援调度等功能于一体的突发化学事故危害评估与救援辅助决策支持系统,可为突发化学事故危害后果评估与辅助救援决策提供有效的技术支撑。展开更多
The origin of the central Qiangtang metamorphic belt(CQMB)has long been in debate,which is not clear whether this belt is the exhumed Jinsha oceanic plate that had been subducted and underthrusted beneath the Qiangtan...The origin of the central Qiangtang metamorphic belt(CQMB)has long been in debate,which is not clear whether this belt is the exhumed Jinsha oceanic plate that had been subducted and underthrusted beneath the Qiangtang Block,or the in situ Longmu Co-Shuanghu suture that separated the south and north Qiangtang blocks.Here we report field observations,zircon U-Pb ages and Lu-Hf isotopes,as well as whole rock geochemistry and Sr-Nd isotopes of the Late Triassic volcanic rocks near the Chabo Co within the southern margin of the CQMB.The ca.229 Ma Chabo Co volcanic rocks and limestones possess characteristic lithologies of a seamount.Their geochemical and isotopic compositions are similar to OIB-type lavas.Unlike other metabasalts(eclogites and blueschists)in the CQMB,the Chabo Co volcanic rocks are OIB-type lavas that did not experience high-grade metamorphism;this is likely because that the Chabo Co seamount was detached from the subducting Longmu Co-Shuanghu oceanic slab.This work provides new solid evidences for an in situ origin of the CQMB.展开更多
基金the support from the National Natural Science Foundation of China (Nos.21974067, 22274073, 81971373 and 82001535)the National Key Research and Development Program of China (No.2018YFC1004700)+1 种基金Fundamental Research Funds for the Central Universities (Nos.020514380309,021414380502 and 2022300324)the State Key Laboratory of Analytical Chemistry for Life Science (Nos.5431ZZXM2305 and 5431ZZXM2204)。
文摘Spermatogenesis, maturation, capacitation and fertilization are precisely regulated by glycosylation. However, the relationship between altered glycosylation patterns and the onset and development of reproductive disorders is unclear, mainly limited by the lack of in situ imaging techniques for spermatozoa glycosylation. We developed an efficient and highly specific spermatozoa glycan imaging technique based on the robust chemoselective labeling of sialic acid(Sia) and N-acetyl-D-galactosamine(Gal/GalNAc). We further proposed a “tandem glycan chemoselective labeling” strategy to achieve simultaneous imaging of two types of glycans on spermatozoa. We applied the developed method to the spermatozoa from oligozoospermic patients and diabetic mice and found that these spermatozoa showed higher levels of Sia and Gal/Gal NAc expression than the normal groups. Moreover, spermatozoa from diabetic mice showed a severe decrease in number, viability, and forward motility, suggesting that in vivo glucose metabolism disorders may lead to an elevated level of spermatozoa glycosylation and have a correlation with the development of oligoasthenotspermia. Our work provides a research tool to reveal the relationship between glycosylation modification and spermatozoa quality, and a promising clue for the development of glycan-based reproductive markers.
基金supported by the National Natural Science Foundation of China(Nos.42007178 and 41907327)the Natural Science Foundation of Hubei(Nos.2020CFB463 and 2019CFB372)+4 种基金China Geological Survey(Nos.DD20160304 and DD20190824)Fundamental Research Funds for the Central Universities(Nos.CUG 190644 and CUGL180817)National Key Research and Development Program(No.2019YFC1805502)Key Laboratory of Karst Dynamics,MNR and GZAR(Institute of Karst Geology,CAGS)Guilin(No.KDL201703)Key Laboratory of Karst Ecosystem and Treatment of Rocky Desertification,MNR and IRCK by UNESCO(No.KDL201903)。
文摘To investigate groundwater flow and solute transport characteristics of the karst trough zone in China,tracer experiments were conducted at two adjacent typical karst groundwater flow systems(Yuquandong(YQD)and Migongquan(MGQ))in Sixi valley,western Hubei,China.Highresolution continuous monitoring was utilized to obtain breakthrough curves(BTCs),which were then analyzed using the multi-dispersion model(MDM)and the two-region nonequilibrium model(2RNE)with basic parameters calculated by CXTFIT and QTRACER2.Results showed that:(1)YQD flow system had a complex infiltration matrix with overland flow,conduit flow and fracture flow,while the MGQ flow system was dominated by conduit flow with fast flow transport velocity,but also small amount of fracture flow there;(2)They were well fitted based on the MDM(R^2=0.928)and 2RNE(R^2=0.947)models,indicating that they had strong adaptability in the karst trough zone;(3)conceptual models for YQD and MGQ groundwater systems were generalized.In YQD system,the solute was transported via overland flow during intense rainfall,while some infiltrated down into fissures and conduits.In MGQ system,most were directly transported to spring outlet in the fissureconduit network.
基金supported by the National Natural Science Foundation of China(No.42372256)the Basic Science Center for Tibetan Plateau Earth System(No.41988101)the Second Tibetan Plateau Scientific Expedition and Research Program(No.2019QZKK0708)。
文摘The Zongwulong Shan-Qinghai Nanshan tectonic belt of the northern Xizang Plateau experienced a protracted tectonica history,including the openings and closures of the Proto-and Paleo-Tethyan Oceans.Although the tectonic belt has been extensively studied,details regarding the tectonic processes involved in its development remain controversial.To better constrain the tectonic processes of this tectonic belt,we conducted detailed field geological mapping,zircon U-Pb geochronology,and whole-rock geochemical and Sr-Nd isotopic analyses.Our results show that intrusive rocks in the tectonic belt crystallized in ca.292-233 Ma,perhaps in an arc/subduction setting.Geochemical and Sr-Nd isotopic data suggest that Early Permian-Late Triassic ultramaficintermediate intrusions were sourced from the enriched mantle,whereas intermediate-acidic rocks were sourced from mixed crust-mantle.We present the tectonic model that involves:(1)Early Devonian-Early Permian intracontinental extension occurred in the northern margin of the Qaidam continent(ca.416-292 Ma);(2)Early Permian-Late Triassic northward subduction of the Paleo-Tethyan Ocean resulted in arc magmatism(ca.292-233 Ma);and(3)subsequent Late Triassic intracontinental extension(ca.233-215 Ma).Our results suggest that the Late Paleozoic-Early Mesozoic development of the Zongwulong Shan-Qinghai Nanshan was related to the opening,subduction,and slab retreat of the Paleo-Tethyan Ocean,which has key implications for the tectonic evolution of the northern Tibetan Plateau.
基金supported by Science and Technology Planning Project of Guangzhou City(2024A04J4474).
文摘Objective:This study aimed to investigate the prevalence,causes,and influencing factors of vision impairment in the elderly population aged 60 years and above in Mangxin Town,Kashgar region,Xinjiang,China.Located in a region characterized by intense ultraviolet radiation and arid climatic conditions,Mangxin Town presents unique environmental challenges that may exacerbate ocular health issues.Despite the global emphasis on addressing vision impairment among aging populations,there remains a paucity of updated and region-specific data in Xinjiang,necessitating this comprehensive assessment to inform targeted interventions.Methods:A cross-sectional study was conducted from May to June 2024,involving 1,311 elderly participants(76.76%participation rate)out of a total eligible population of 1,708 individuals aged≥60 years.Participants underwent detailed ocular examinations,including assessments of uncorrected visual acuity(UVA)and best-corrected visual acuity(BCVA)using standard logarithmic charts,slit-lamp biomicroscopy,optical coherence tomography(OCT,Topcon DRI OCT Triton),fundus photography,and intraocular pressure measurement(Canon TX-20 Tonometer).A multidisciplinary team of 10 ophthalmologists and 2 local village doctors,trained rigorously in standardized protocols,ensured consistent data collection.Demographic,lifestyle,and medical history data were collected via questionnaires.Statistical analyses,performed using STATA 16,included multivariate logistic regression to identify risk factors,with significance defined as P<0.05.Results:The overall prevalence of vision impairment was 13.21%(95%CI:11.37%-15.04%),with low vision at 11.76%(95%CI:10.01%-13.50%)and blindness at 1.45%(95%CI:0.80%-2.10%).Cataract emerged as the leading cause,responsible for 68.20%of cases,followed by glaucoma(5.80%),optic atrophy(5.20%),and age-related macular degeneration(2.90%).Vision impairment prevalence escalated significantly with age:7.74%in the 60–69 age group,17.79%in 70–79,and 33.72%in those≥80.Males exhibited higher prevalence than females(15.84%vs.10.45%,P=0.004).Multivariate analysis revealed age≥80 years(OR=6.43,95%CI:3.79%-10.90%),male sex(OR=0.53,95%CI:0.34%-0.83%),and daily exercise(OR=0.44,95%CI:0.20%-0.95%)as significant factors.History of eye disease showed a non-significant trend toward increased risk(OR=1.49,P=0.107).Education level,income,and smoking status showed no significant associations.Conclusions:This study underscores cataract as the predominant cause of vision impairment in Mangxin Town’s elderly population,with age and sex as critical determinants.The findings align with global patterns but highlight region-specific challenges,such as environmental factors contributing to cataract prevalence.Public health strategies should prioritize improving access to cataract surgery,enhancing grassroots ophthalmic infrastructure,and integrating portable screening technologies for early detection of fundus diseases.Additionally,promoting health education on UV protection and lifestyle modifications,such as regular exercise,may mitigate risks.Future research should expand to broader regions in Xinjiang,employ advanced diagnostic tools for complex conditions like glaucoma,and explore longitudinal trends to refine intervention strategies.These efforts are vital to reducing preventable blindness and improving quality of life for aging populations in underserved areas.
文摘化学事故具有突发性强、环境污染破坏严重、救援难度大等特点,对突发化学事故的影响范围、破坏程度进行快速评估,为事故抢险救援提供决策支持,是应对重特大突发化学事故、降低事故损失的重要手段。本文基于三维地理信息系统(Three-dimensional Geographic Information System,3D GIS),在充分考虑下垫面影响的前提下,研究了大气污染扩散模型,提出了扩散场快速构建技术及兼顾污染分区的救援力量调度方法,并以此为核心建立了系统总体框架,形成了集扩散模拟、危害分析、救援调度等功能于一体的突发化学事故危害评估与救援辅助决策支持系统,可为突发化学事故危害后果评估与辅助救援决策提供有效的技术支撑。
基金financially supported by the National Natural Science Foundation of China (Nos. 41702212,41672054)the Fundamental Research Funds for Central Universities,China University of Geosciences (Wuhan) (Nos. CUGL170816 and CUGQYZX1745)
文摘The origin of the central Qiangtang metamorphic belt(CQMB)has long been in debate,which is not clear whether this belt is the exhumed Jinsha oceanic plate that had been subducted and underthrusted beneath the Qiangtang Block,or the in situ Longmu Co-Shuanghu suture that separated the south and north Qiangtang blocks.Here we report field observations,zircon U-Pb ages and Lu-Hf isotopes,as well as whole rock geochemistry and Sr-Nd isotopes of the Late Triassic volcanic rocks near the Chabo Co within the southern margin of the CQMB.The ca.229 Ma Chabo Co volcanic rocks and limestones possess characteristic lithologies of a seamount.Their geochemical and isotopic compositions are similar to OIB-type lavas.Unlike other metabasalts(eclogites and blueschists)in the CQMB,the Chabo Co volcanic rocks are OIB-type lavas that did not experience high-grade metamorphism;this is likely because that the Chabo Co seamount was detached from the subducting Longmu Co-Shuanghu oceanic slab.This work provides new solid evidences for an in situ origin of the CQMB.
