BACKGROUND： Interferon-gamma （IFN-γ） can make neurons in basal forebrain and septal nuclei differentiate into cholinergic neurons by treating the cells in cerebral cortex of newborn rats, without the inhibition fr...BACKGROUND： Interferon-gamma （IFN-γ） can make neurons in basal forebrain and septal nuclei differentiate into cholinergic neurons by treating the cells in cerebral cortex of newborn rats, without the inhibition from IFN-γ antibody. The important effect of IFN-γ on the development and differentiation of neurons has been found by some scholars. OBJ EClIVE：To investigate whether IFN-γ has differentiational effect on cholinergic neurons in basal forebrain and septal nuclei, and make clear that the increased number of cholinergic neurons is resulted by cell differentiation or cell proliferation. DESIGN ： Controlled observation trial SETTING： Department of Cell Biology, Medical School, Beijing University MATERIALS： Sixty-eight female Wistar rats at embryonic 16 days, weighing 250 to 350 g, were enrolled in this study, and they were provided by the Experimental Animal Center, Medical School, Beijing University. IFN-γ was the product of Gibco Company. METHODS： This study was carried out in the Department of Cell Biology, Medical School, Beijing University and Daheng Image Company of Chinese Academy of Sciences during September 1995 to December 2002. The female Wistar rats at embryonic 16 days were sacrificed, and their fetuses were taken out. Primary culture of the isolated basal forebrain and septal nuclei was performed. The cultured nerve cells were assigned into 3 groups： control group （nothing added）, IFN-γ group（1×10^5 U/L interferon）, IFN-γ＋ IFN-γ antibody group （1 ×10^5 U/L IFN-γ ＋ IFN-γ antibody）. The specific marker enzyme （choline acetyl transferase） of cholinergic neuron was stained with immunohistochemical method. Choline acetyl transferase positive cells were counted, and ^14C-acetyl CoA was used as substrate to detect the activity of choline acetyl transferase, so as to reflect the differentiational effect of IFN-γ on cholinergic neuron in basal forebrain and septal nuclei. Flow cytometry was used to analyze cell circle and detect the proliferation of nerve cells. Nerve cells were marked with MAP2 and counted to evaluate the neuronal proliferation in basal forebrain and septal nuclei. MAIN OUTCOME MEASURES： Effect of interferon-γ on the number and activity of choline acetyl transferase-positive ceils in basal forebrain and septal nuclei as well as the effect on neuronal proliferation. RESULTS ： ① Nerve cells in the basal forebrain and septal nuclei of IFN-γ group grew well compared with control group.②The differentiation of cholinergic neurons： The number and activity of choline acetyl transferase positive cells in IFN-γ group were significantly higher than those in the control group [（49.30 ±4.92） /100 cells vs （7.50±1.58） /100 cells; （2 049.00±12.30） min^-1 vs （1 227.30±12.59） min^-1, p 〈 0.01], while there was no significant difference in the number and activity of choline acetyl transferase positive cells between IFN-γ ＋ IFN-γ antibody group and control group（P 〉 0.05）. ③The proliferation of cholinergic neurons： Cell percentage was 17.2% and 19.8% at S-stage, 6.2% and 6.1% at G2＋M stage in the control group and IFN-γ group respectively, without significant difference （P 〉 0.05）. CONCLUSION ： IFN-γ does not promote the neuronal proliferation in basal forebrain and septal nuclei, and the increased expression of cholinergic neurons is not resulted by the increase in the number of neurons, but its differentiation.展开更多
The cermet composites WC-20wt%Co/ZrO2（E6）with four different comtents of ZrO2（3Y）were prepared by normal vacuum sinter processing;the optical microscope and SEMwere used to characterize their microstructures.The h...The cermet composites WC-20wt%Co/ZrO2（E6）with four different comtents of ZrO2（3Y）were prepared by normal vacuum sinter processing;the optical microscope and SEMwere used to characterize their microstructures.The hardness.bending strength and impact toughness of the specimens were determined.The experimental results show that ZrO2（3Y） particles in WC-20wt%Co matrix are sphcrical particles in different sizes which are distributed uniformly in Co phases and WC phases,the bending strength and impact toughness of the WC-20wt%Co cermet composites added ZrO2（3Y）improve remarkably.but the hardness values have little change.展开更多
Objective:To analyze the effect of apatinib,S-1 combined with paclitaxel perfusion on malignant seroperitoneum of gastric cancer.Methods:From December 2019 to May 2020,172 patients with gastric cancer treated in our h...Objective:To analyze the effect of apatinib,S-1 combined with paclitaxel perfusion on malignant seroperitoneum of gastric cancer.Methods:From December 2019 to May 2020,172 patients with gastric cancer treated in our hospital were randomly divided into two groups:observation group and control group,86 cases each.The control group adopted the method of S-1 combined with paclitaxel perfusion therapy in the treatment of malignant seroperitoneum of gastric cancer.The observation group was given oral apatinib on the basis of S-1 combined with paclitaxel perfusion therapy,and the dosage was 500 mg/d.Results:The total effective treatment in the control group was 43.02%,while the total effective rate in the observation group was 69.77%;the drug resistance of the two groups of patients increased and the adverse reactions were low.Conclusion:Apatinib and S-1 combined with paclitaxel perfusion therapy can effectively improve the treatment effect,stabilize the patient's condition,increase the patient's drug resistance to adverse reactions,and have a good prognosis.展开更多
BACKGROUND： The supernatant of interferon-gamma （IFNγ） co-cultured with neonatal rat cortical glia can promote the cells in embryonic basal forebrain/septal nuclei to differentiate into cholinergic neurons, but th...BACKGROUND： The supernatant of interferon-gamma （IFNγ） co-cultured with neonatal rat cortical glia can promote the cells in embryonic basal forebrain/septal nuclei to differentiate into cholinergic neurons, but the mechanism is still unclear. OBJECTIVE： To analyze the pathways for IFNγ to promote the differentiation of primarily cultured cholinergic neurons in rat embryonic basal forebrain/septal nuclei through culture in different conditioned medium. DESIGN： A controlled experiment taking cells as the observational target. SETTINGS： Department of Biochemistry and Molecular Biology, Youjiang Medical College for Nationalities; Department of Cell Biology, Beijing University Health Science Center. MATERIALS： Sixty-four pregnant Wistar rats for 16 days （250-350 g） and 84 Wistar rats （either male or female, 5-7 g） of 0-1 day after birth were provided by the experimental animal department of Beijing University Health Science Center. Rat IFNγ were provided by Gibco Company; Glial fibrillary acidic protein by Huamei Company. METHODS： The experiments were carried out in the Department of Cell Biology, Beijing University Health Science Center and Daheng Image Company of Chinese Academy of Science from July 1995 to December 2002. ① Interventions： The nerve cells in the basal forebrain/septal nuclei of the pregnant Wistar rats for 16 days were primarily cultured, and then divided into four groups： Blank control group （not any supernatant and medium was added）; Control group （added by mixed glial cell or astrocyte conditioned medium）; IFNγ group （added by mixed glial cell or astrocyte conditioned medium＋IFNγ）. Antibody group （added by mixed glial cell or astrocyte conditioned medium＋IFNγ＋Ab-IFNγ）. Mixed glial cell or astrocyte conditioned medium was prepared using cerebral cortex of Wistar rats of 0-1 day after birth. ② Evaluation： The immunohistochemical method was used to perform the choline acetyltransferase （ChAT） staining of cholinergic neurons. The ChAT positive cells were counted. MAIN OUTCOME MEASURES： Comparison of ChAT positive cells in rat basal forebrain and septal nuclei in different conditioned medium. RESULTS： ① ChAT positive cells in mixed glial cell conditioned medium： The ChAT positive cells in the IFNγ group and antibody group were significantly more than those in the control group （P 〈 0.01）. ② ChAT positive cells in astrocyte conditioned medium： The ChAT positive cells in the IFNγ group were significantly more than those in the control group, but there was no significant difference between the antibody group and control group （P 〉 0.05）. CONCLUSION： IFNγ cannot directly promote the differentiation of cholinergic neurons, but plays a role through activating glial cells （except astrocytes） to produce IFNγ like molecules.展开更多
Phosphate coatings are partially dissolved in the cathodic area during the cathode-electrophoretic painting processes causing the result that the coatings adhesion,corrosion protection,alkaline resistance are decrease...Phosphate coatings are partially dissolved in the cathodic area during the cathode-electrophoretic painting processes causing the result that the coatings adhesion,corrosion protection,alkaline resistance are decreased.Furthermore,the service life of the coated production will be shortened.The addition of Ni2+,Mn2+to the phosphating bath to improve the corrosion stability,alkaline resistance,as well as the adhesion of coating layers is studied in the article.These heavy metals do not only catalyse the surface reactions but generate a more corrosion resistant surface.We perfected the trecation phosphate solution by reducing the sludge,accelerating the phosphating reactions,improving corrosion protection and alkaline resistance.More even and finer grain structures,excellent corrosion resistance were obtained compared to the traditional phosphating technique.展开更多
为提升船用汽轮发电机组大幅变工况时的控制精度和鲁棒性,以船用汽轮机调节系统各部件的模块化数学模型为基础,建立汽轮机组数字电液(digital electric hydraulic,DEH)闭环模糊比例、积分、微分(proportion integration derivative,PID...为提升船用汽轮发电机组大幅变工况时的控制精度和鲁棒性,以船用汽轮机调节系统各部件的模块化数学模型为基础,建立汽轮机组数字电液(digital electric hydraulic,DEH)闭环模糊比例、积分、微分(proportion integration derivative,PID)控制模型;融合遗传算法的选择、交叉、变异和自适应递减权重法,提出遗传粒子群智能优化算法,并结合标准测试函数验证提出算法具有较高的收敛速度和精度;基于遗传粒子群智能优化算法建立汽轮机变工况自适应智能模糊PID控制模型,实现模糊PID的量化因子与比例因子最优化设计,进而开展船用汽轮发电机组大幅变工况动态特性及扰动因素影响分析,结果表明本文建立的自适应智能模糊PID控制模型具有更好的控制稳态性能与鲁棒性,为船用汽轮机组大幅度变工况智能控制优化设计提供了有力的技术支撑。展开更多
基金the National Nat-ural Science Foundation of Chi-na, No.39570249
文摘BACKGROUND： Interferon-gamma （IFN-γ） can make neurons in basal forebrain and septal nuclei differentiate into cholinergic neurons by treating the cells in cerebral cortex of newborn rats, without the inhibition from IFN-γ antibody. The important effect of IFN-γ on the development and differentiation of neurons has been found by some scholars. OBJ EClIVE：To investigate whether IFN-γ has differentiational effect on cholinergic neurons in basal forebrain and septal nuclei, and make clear that the increased number of cholinergic neurons is resulted by cell differentiation or cell proliferation. DESIGN ： Controlled observation trial SETTING： Department of Cell Biology, Medical School, Beijing University MATERIALS： Sixty-eight female Wistar rats at embryonic 16 days, weighing 250 to 350 g, were enrolled in this study, and they were provided by the Experimental Animal Center, Medical School, Beijing University. IFN-γ was the product of Gibco Company. METHODS： This study was carried out in the Department of Cell Biology, Medical School, Beijing University and Daheng Image Company of Chinese Academy of Sciences during September 1995 to December 2002. The female Wistar rats at embryonic 16 days were sacrificed, and their fetuses were taken out. Primary culture of the isolated basal forebrain and septal nuclei was performed. The cultured nerve cells were assigned into 3 groups： control group （nothing added）, IFN-γ group（1×10^5 U/L interferon）, IFN-γ＋ IFN-γ antibody group （1 ×10^5 U/L IFN-γ ＋ IFN-γ antibody）. The specific marker enzyme （choline acetyl transferase） of cholinergic neuron was stained with immunohistochemical method. Choline acetyl transferase positive cells were counted, and ^14C-acetyl CoA was used as substrate to detect the activity of choline acetyl transferase, so as to reflect the differentiational effect of IFN-γ on cholinergic neuron in basal forebrain and septal nuclei. Flow cytometry was used to analyze cell circle and detect the proliferation of nerve cells. Nerve cells were marked with MAP2 and counted to evaluate the neuronal proliferation in basal forebrain and septal nuclei. MAIN OUTCOME MEASURES： Effect of interferon-γ on the number and activity of choline acetyl transferase-positive ceils in basal forebrain and septal nuclei as well as the effect on neuronal proliferation. RESULTS ： ① Nerve cells in the basal forebrain and septal nuclei of IFN-γ group grew well compared with control group.②The differentiation of cholinergic neurons： The number and activity of choline acetyl transferase positive cells in IFN-γ group were significantly higher than those in the control group [（49.30 ±4.92） /100 cells vs （7.50±1.58） /100 cells; （2 049.00±12.30） min^-1 vs （1 227.30±12.59） min^-1, p 〈 0.01], while there was no significant difference in the number and activity of choline acetyl transferase positive cells between IFN-γ ＋ IFN-γ antibody group and control group（P 〉 0.05）. ③The proliferation of cholinergic neurons： Cell percentage was 17.2% and 19.8% at S-stage, 6.2% and 6.1% at G2＋M stage in the control group and IFN-γ group respectively, without significant difference （P 〉 0.05）. CONCLUSION ： IFN-γ does not promote the neuronal proliferation in basal forebrain and septal nuclei, and the increased expression of cholinergic neurons is not resulted by the increase in the number of neurons, but its differentiation.
文摘The cermet composites WC-20wt%Co/ZrO2（E6）with four different comtents of ZrO2（3Y）were prepared by normal vacuum sinter processing;the optical microscope and SEMwere used to characterize their microstructures.The hardness.bending strength and impact toughness of the specimens were determined.The experimental results show that ZrO2（3Y） particles in WC-20wt%Co matrix are sphcrical particles in different sizes which are distributed uniformly in Co phases and WC phases,the bending strength and impact toughness of the WC-20wt%Co cermet composites added ZrO2（3Y）improve remarkably.but the hardness values have little change.
基金Apatinib,S-1 combined with paclitaxel perfusion in the treatment of malignant seroperitoneum of gastric cancer(No.of project:17ZF165)。
文摘Objective:To analyze the effect of apatinib,S-1 combined with paclitaxel perfusion on malignant seroperitoneum of gastric cancer.Methods:From December 2019 to May 2020,172 patients with gastric cancer treated in our hospital were randomly divided into two groups:observation group and control group,86 cases each.The control group adopted the method of S-1 combined with paclitaxel perfusion therapy in the treatment of malignant seroperitoneum of gastric cancer.The observation group was given oral apatinib on the basis of S-1 combined with paclitaxel perfusion therapy,and the dosage was 500 mg/d.Results:The total effective treatment in the control group was 43.02%,while the total effective rate in the observation group was 69.77%;the drug resistance of the two groups of patients increased and the adverse reactions were low.Conclusion:Apatinib and S-1 combined with paclitaxel perfusion therapy can effectively improve the treatment effect,stabilize the patient's condition,increase the patient's drug resistance to adverse reactions,and have a good prognosis.
基金the National Natural Science Foundation of China, No. 39570249
文摘BACKGROUND： The supernatant of interferon-gamma （IFNγ） co-cultured with neonatal rat cortical glia can promote the cells in embryonic basal forebrain/septal nuclei to differentiate into cholinergic neurons, but the mechanism is still unclear. OBJECTIVE： To analyze the pathways for IFNγ to promote the differentiation of primarily cultured cholinergic neurons in rat embryonic basal forebrain/septal nuclei through culture in different conditioned medium. DESIGN： A controlled experiment taking cells as the observational target. SETTINGS： Department of Biochemistry and Molecular Biology, Youjiang Medical College for Nationalities; Department of Cell Biology, Beijing University Health Science Center. MATERIALS： Sixty-four pregnant Wistar rats for 16 days （250-350 g） and 84 Wistar rats （either male or female, 5-7 g） of 0-1 day after birth were provided by the experimental animal department of Beijing University Health Science Center. Rat IFNγ were provided by Gibco Company; Glial fibrillary acidic protein by Huamei Company. METHODS： The experiments were carried out in the Department of Cell Biology, Beijing University Health Science Center and Daheng Image Company of Chinese Academy of Science from July 1995 to December 2002. ① Interventions： The nerve cells in the basal forebrain/septal nuclei of the pregnant Wistar rats for 16 days were primarily cultured, and then divided into four groups： Blank control group （not any supernatant and medium was added）; Control group （added by mixed glial cell or astrocyte conditioned medium）; IFNγ group （added by mixed glial cell or astrocyte conditioned medium＋IFNγ）. Antibody group （added by mixed glial cell or astrocyte conditioned medium＋IFNγ＋Ab-IFNγ）. Mixed glial cell or astrocyte conditioned medium was prepared using cerebral cortex of Wistar rats of 0-1 day after birth. ② Evaluation： The immunohistochemical method was used to perform the choline acetyltransferase （ChAT） staining of cholinergic neurons. The ChAT positive cells were counted. MAIN OUTCOME MEASURES： Comparison of ChAT positive cells in rat basal forebrain and septal nuclei in different conditioned medium. RESULTS： ① ChAT positive cells in mixed glial cell conditioned medium： The ChAT positive cells in the IFNγ group and antibody group were significantly more than those in the control group （P 〈 0.01）. ② ChAT positive cells in astrocyte conditioned medium： The ChAT positive cells in the IFNγ group were significantly more than those in the control group, but there was no significant difference between the antibody group and control group （P 〉 0.05）. CONCLUSION： IFNγ cannot directly promote the differentiation of cholinergic neurons, but plays a role through activating glial cells （except astrocytes） to produce IFNγ like molecules.
文摘Phosphate coatings are partially dissolved in the cathodic area during the cathode-electrophoretic painting processes causing the result that the coatings adhesion,corrosion protection,alkaline resistance are decreased.Furthermore,the service life of the coated production will be shortened.The addition of Ni2+,Mn2+to the phosphating bath to improve the corrosion stability,alkaline resistance,as well as the adhesion of coating layers is studied in the article.These heavy metals do not only catalyse the surface reactions but generate a more corrosion resistant surface.We perfected the trecation phosphate solution by reducing the sludge,accelerating the phosphating reactions,improving corrosion protection and alkaline resistance.More even and finer grain structures,excellent corrosion resistance were obtained compared to the traditional phosphating technique.
文摘目的:探讨采用孟德尔随机化(Mendelian randomization,MR)研究探讨特应性疾病(哮喘和特应性皮炎)与骨关节炎(osteoarthritis,OA)之间的因果关系。方法:选择哮喘和特应性皮炎作为工具变量,通过IEU数据库进行检索,选择最新及病例数、单核苷酸多态性(single nucleotide polymorphism,SNP)数量较多的数据,使用R语言对数据进行收集处理,采用逆方差加权(inverse-varianceweighted,IVW)方法作为主要的MR评估方法,根据全基因组关联研究(genome-wide association studies,GWAS)中膝及髋关节汇总数据进行单一线性回归估计因果关系。绘制森林图对结果进行可视化处理,散点图、漏斗图等进行基因多效性分析及敏感性分析。同时选择哮喘、特应性皮炎、身体质量指数(body mass index,BMI)、骨质疏松症与OA进行多变量MR分析排除GWAS数据中水平多效性对结果的影响。结果:MR-IVW结果显示,哮喘与OA存在因果关系[OR=1.41,95%CI(1.07,1.85),P=0.02],使用多变量孟德尔随机化法(multivariate Mendelian randomization,MVMR)对BMI、骨质疏松症进行调整后,结果发现哮喘对OA的因果效应保持不变[OR=1.57,95%CI(1.03,2.39),P=0.03]。特应性皮炎与OA的两样本MR结果为[OR=1.01,95%CI(0.97,1.04),P=0.76],MVMR结果为[OR=1.02,95%CI(0.99,1.05),P=0.25],均提示二者无明确的因果关系。讨论:哮喘会增加OA的发病风险,特应性皮炎与OA的发病无明显关系,特应性疾病与OA的关系仍需讨论。
文摘为提升船用汽轮发电机组大幅变工况时的控制精度和鲁棒性,以船用汽轮机调节系统各部件的模块化数学模型为基础,建立汽轮机组数字电液(digital electric hydraulic,DEH)闭环模糊比例、积分、微分(proportion integration derivative,PID)控制模型;融合遗传算法的选择、交叉、变异和自适应递减权重法,提出遗传粒子群智能优化算法,并结合标准测试函数验证提出算法具有较高的收敛速度和精度;基于遗传粒子群智能优化算法建立汽轮机变工况自适应智能模糊PID控制模型,实现模糊PID的量化因子与比例因子最优化设计,进而开展船用汽轮发电机组大幅变工况动态特性及扰动因素影响分析,结果表明本文建立的自适应智能模糊PID控制模型具有更好的控制稳态性能与鲁棒性,为船用汽轮机组大幅度变工况智能控制优化设计提供了有力的技术支撑。
