Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARSCoV-2),has affected more than 600 million people worldwide.Several organs including lung,intestine,and brain are infecte...Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARSCoV-2),has affected more than 600 million people worldwide.Several organs including lung,intestine,and brain are infected by SARS-CoV-2.It has been reported that SARS-CoV-2 receptor angiotensin-converting enzyme-2(ACE2)is expressed in human testis.However,whether testis is also affected by SARS-CoV-2 is still unclear.In this study,we generate a human ACE2(hACE2)transgenic mouse model in which the expression of hACE2 gene is regulated by hACE2 promoter.Sertoli and Leydig cells from hACE2 transgenic mice can be infected by SARS-CoV-2 pseudovirus in vitro,and severe pathological changes are observed after injecting the SARS-CoV-2 pseudovirus into the seminiferous tubules.Further studies reveal that Sertoli and Leydig cells from hACE2 transgenic mice are also infected by authentic SARS-CoV-2 virus in vitro.After testis interstitium injection,authentic SARS-CoV-2 viruses are first disseminated to the interstitial cells,and then detected inside the seminiferous tubules which in turn cause germ cell loss and disruption of seminiferous tubules.Our study demonstrates that testis is most likely a target of SARS-CoV-2 virus.Attention should be paid to the reproductive function in SARS-CoV-2 patients.展开更多
Dear Editor,Plant expression vectors are instrumental tools used to assess plant functional genomics and facilitate breeding improvements.Plant viruses may contain exogenous genes for replication and systemic movement...Dear Editor,Plant expression vectors are instrumental tools used to assess plant functional genomics and facilitate breeding improvements.Plant viruses may contain exogenous genes for replication and systemic movement in host plants,allowing them to be engineered into plant expression vectors for systemic and enhanced gene expression manipulation(Abrahamian et al.,2020).展开更多
基金supported by National key R&D program of China(2018YFA0107700)the National Natural Science Foundation of China(32170855,31970785)Biological Resources Program of Chinese Academy of Sciences(KFJ-BRP-005).
文摘Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARSCoV-2),has affected more than 600 million people worldwide.Several organs including lung,intestine,and brain are infected by SARS-CoV-2.It has been reported that SARS-CoV-2 receptor angiotensin-converting enzyme-2(ACE2)is expressed in human testis.However,whether testis is also affected by SARS-CoV-2 is still unclear.In this study,we generate a human ACE2(hACE2)transgenic mouse model in which the expression of hACE2 gene is regulated by hACE2 promoter.Sertoli and Leydig cells from hACE2 transgenic mice can be infected by SARS-CoV-2 pseudovirus in vitro,and severe pathological changes are observed after injecting the SARS-CoV-2 pseudovirus into the seminiferous tubules.Further studies reveal that Sertoli and Leydig cells from hACE2 transgenic mice are also infected by authentic SARS-CoV-2 virus in vitro.After testis interstitium injection,authentic SARS-CoV-2 viruses are first disseminated to the interstitial cells,and then detected inside the seminiferous tubules which in turn cause germ cell loss and disruption of seminiferous tubules.Our study demonstrates that testis is most likely a target of SARS-CoV-2 virus.Attention should be paid to the reproductive function in SARS-CoV-2 patients.
基金supported by the Biological Breeding of Early Maturing and Disease Resistant Cotton Varieties(grant no.2023ZD04041)the China Agricultural Research System(grant no.CARS-15-06)+1 种基金the Natural Science Foundation of Henan Province(grant no.232300421041)the Nanfan Special Project(grant nos.YBXM2316 and YBXM2441).
文摘Dear Editor,Plant expression vectors are instrumental tools used to assess plant functional genomics and facilitate breeding improvements.Plant viruses may contain exogenous genes for replication and systemic movement in host plants,allowing them to be engineered into plant expression vectors for systemic and enhanced gene expression manipulation(Abrahamian et al.,2020).
