Objective: This study aimed to evaluate the prevalence and related factors of post-traumatic stress disorder (PTSD) symptoms among doctors and nurses who were exposed to H7N9 patients during the H7N9 influenza epidemi...Objective: This study aimed to evaluate the prevalence and related factors of post-traumatic stress disorder (PTSD) symptoms among doctors and nurses who were exposed to H7N9 patients during the H7N9 influenza epidemic.To provide scientific basis for promoting the physical and psychological health of these staff members.Method: The 102 medical staff workers who were exposed to H7N9 patients were recruited through convenient sampling between January 2015 and May 2016.We used a self-reported questionnaire,the PTSD Checklist-Civilian Version (PCL-C),to evaluate the PTSD symptoms among doctors and nurses from an intensive care unit (n =61),a respiratory department (n =20),and an emergency department (n =21).We then analyzed the related factors.Results: Around 20.59% of the tested doctors and nurses showed PTSD symptoms.The sample had a mean PCL-C score of 30.00 ± 9.95.The differences in the scores of doctors and nurses with different genders,ages,professional titles,contact frequencies,trainings,and experiences were statistically significant (P < 0.05,P < 0.01).Moreover,t-tests and one-way analysis of variance showed that nurses received higher scores than doctors,female participants received higher scores than male participants,and the participants with low professional title and high contact frequency,aged between 20 years and 30 years,with less than five years of work experience,having not received related training and with no related experience obtained higher PCL-C scores than the others (P < 0.05,P < 0.01).Conclusion: The PTSD level of doctors and nurses after their exposure to H7N9 patients was high,which warrant further research.Health and medical institutions should pay attention to the physical and psychological health of these staff members.展开更多
Competing endogenous RNAs(ce RNAs)containing micro RNA response elements can competitively interact with micro RNA via mi RNA response elements,which can combine non-coding RNAs with protein-coding RNAs through comple...Competing endogenous RNAs(ce RNAs)containing micro RNA response elements can competitively interact with micro RNA via mi RNA response elements,which can combine non-coding RNAs with protein-coding RNAs through complex ce RNA networks.Ce RNAs include non-coding RNAs(long non-coding RNAs,circular RNAs,and transcribed pseudogenes)and protein-coding RNAs(m RNAs).Molecular interactions in ce RNA networks can coordinate many biological processes;however,they may also lead to ce RNA network imbalance and thus contribute to cancer occurrence when disturbed.Recent studies indicate that many dysregulated RNAs derived from lung cancer may function as ce RNAs to regulate multitudinous biological functions for lung cancer,including tumor cell proliferation,apoptosis,growth,invasion,migration,and metastasis.This study therefore reviewed the research progress in the field of non-coding and protein-coding RNAs as ce RNAs in lung cancer,and highlighted validated ce RNAs involved in biological lung cancer functions.Furthermore,the roles of ce RNAs as novel prognostic and diagnostic biomarkers were also discussed.Interpreting the involvement of ce RNAs networks in lung cancer will provide new insight into cancer pathogenesis and treatment strategies.展开更多
Lung cancer is emerging as a common malignancy worldwide,with non-small cell lung cancer(NSCLC)accounting for approximately 85%of all cases.Two-dimensional(2D)in vitro cell line cultures and animal models are currentl...Lung cancer is emerging as a common malignancy worldwide,with non-small cell lung cancer(NSCLC)accounting for approximately 85%of all cases.Two-dimensional(2D)in vitro cell line cultures and animal models are currently used to study NSCLC.However,2D cell cultures fail to replicate the medication response and neoplastic heterogeneity of parental tumors.Animal models are expensive and require lengthy modeling cycles.The generation of in vitro three-dimensional(3D)tissue cultures called organoids,which exhibit multicellular,anatomical,and functional properties of real organs,is now achievable owing to advancements in stem cell culturing.The genetic,proteomic,morphological,and pharmacological characteristics of tumors are largely preserved in tumor organoids grown in vitro.The design and physiology of human organs can be precisely reconstructed in tumor organoids,opening new possibilities for complementing the use of animal models and studying human diseases.This review summarizes the development of NSCLC organoids and their applications in basic research,drug testing,immunotherapy,and individualized treatments.展开更多
文摘Objective: This study aimed to evaluate the prevalence and related factors of post-traumatic stress disorder (PTSD) symptoms among doctors and nurses who were exposed to H7N9 patients during the H7N9 influenza epidemic.To provide scientific basis for promoting the physical and psychological health of these staff members.Method: The 102 medical staff workers who were exposed to H7N9 patients were recruited through convenient sampling between January 2015 and May 2016.We used a self-reported questionnaire,the PTSD Checklist-Civilian Version (PCL-C),to evaluate the PTSD symptoms among doctors and nurses from an intensive care unit (n =61),a respiratory department (n =20),and an emergency department (n =21).We then analyzed the related factors.Results: Around 20.59% of the tested doctors and nurses showed PTSD symptoms.The sample had a mean PCL-C score of 30.00 ± 9.95.The differences in the scores of doctors and nurses with different genders,ages,professional titles,contact frequencies,trainings,and experiences were statistically significant (P < 0.05,P < 0.01).Moreover,t-tests and one-way analysis of variance showed that nurses received higher scores than doctors,female participants received higher scores than male participants,and the participants with low professional title and high contact frequency,aged between 20 years and 30 years,with less than five years of work experience,having not received related training and with no related experience obtained higher PCL-C scores than the others (P < 0.05,P < 0.01).Conclusion: The PTSD level of doctors and nurses after their exposure to H7N9 patients was high,which warrant further research.Health and medical institutions should pay attention to the physical and psychological health of these staff members.
基金supported by the National Natural Science Foundation of China(Nos.31670952 and 81902796)。
文摘Competing endogenous RNAs(ce RNAs)containing micro RNA response elements can competitively interact with micro RNA via mi RNA response elements,which can combine non-coding RNAs with protein-coding RNAs through complex ce RNA networks.Ce RNAs include non-coding RNAs(long non-coding RNAs,circular RNAs,and transcribed pseudogenes)and protein-coding RNAs(m RNAs).Molecular interactions in ce RNA networks can coordinate many biological processes;however,they may also lead to ce RNA network imbalance and thus contribute to cancer occurrence when disturbed.Recent studies indicate that many dysregulated RNAs derived from lung cancer may function as ce RNAs to regulate multitudinous biological functions for lung cancer,including tumor cell proliferation,apoptosis,growth,invasion,migration,and metastasis.This study therefore reviewed the research progress in the field of non-coding and protein-coding RNAs as ce RNAs in lung cancer,and highlighted validated ce RNAs involved in biological lung cancer functions.Furthermore,the roles of ce RNAs as novel prognostic and diagnostic biomarkers were also discussed.Interpreting the involvement of ce RNAs networks in lung cancer will provide new insight into cancer pathogenesis and treatment strategies.
基金supported by the National Natural Science Foundation of China(No.32271363 and No.82172831)the Natural Science Foundation of Chongqing(No.cstc2021jcyj-msxmX0642).
文摘Lung cancer is emerging as a common malignancy worldwide,with non-small cell lung cancer(NSCLC)accounting for approximately 85%of all cases.Two-dimensional(2D)in vitro cell line cultures and animal models are currently used to study NSCLC.However,2D cell cultures fail to replicate the medication response and neoplastic heterogeneity of parental tumors.Animal models are expensive and require lengthy modeling cycles.The generation of in vitro three-dimensional(3D)tissue cultures called organoids,which exhibit multicellular,anatomical,and functional properties of real organs,is now achievable owing to advancements in stem cell culturing.The genetic,proteomic,morphological,and pharmacological characteristics of tumors are largely preserved in tumor organoids grown in vitro.The design and physiology of human organs can be precisely reconstructed in tumor organoids,opening new possibilities for complementing the use of animal models and studying human diseases.This review summarizes the development of NSCLC organoids and their applications in basic research,drug testing,immunotherapy,and individualized treatments.
