Long-term exposure to monochromatic light during early life has been shown to significantly impact the development of myopia.However,its effect on color processing in the visual cortex remains unclear.In this study,we...Long-term exposure to monochromatic light during early life has been shown to significantly impact the development of myopia.However,its effect on color processing in the visual cortex remains unclear.In this study,we investigated the effects of different lighting conditions on the functional organization of color representation in the visual cortical areas V1,V2,and V4 of rhesus monkeys raised under long-wave and short-wave monochromatic illumination for four years.Using cytochrome oxidase staining and intrinsic signal optical imaging,we found that the sizes,densities,and response strengths of cortical color domains in V1,V2,and V4 were consistent across illumination conditions.In addition,the cortical distances between specific hue response patches did not significantly differ among the groups.These findings suggested that long-term monochromatic illumination does not alter the spatial organization or functional properties of color domains in the visual cortex of rhesus monkeys.This research provides new insights into the resilience of the visual system's chromatic representation despite altered lighting conditions in early life.展开更多
Here,we present integrated nanorod arrays on microfluidic chips for fast and sensitive flow-through immunoassays of physiologically relevant macromolecules.Dense arrays of Au nanorods are easily fabricated through one...Here,we present integrated nanorod arrays on microfluidic chips for fast and sensitive flow-through immunoassays of physiologically relevant macromolecules.Dense arrays of Au nanorods are easily fabricated through one-step oblique angle deposition,which eliminates the requirement of advanced lithography methods.We report the utility of this plasmonic structure to improve the detection limit of the cardiac troponin I(cTnI)assay by over 6x 105-fold,reaching down to 33.9fg mL^(-1)(-1.4fM) compared with an identical assay on glass substrates.Through monolithic integration with microfluidic elements,the device enables a flow-through assay for quantitative detection of cTnI in the serum with a detection sensitivity of 6.9 pg mL^(-1)(-0.3 pM)in<6 min,which was 4000 times lower than conventional glass devices.This ultrasensitive detection arises from the large surface area for antibody conjugation and metal-enhanced fluorescent signals through plasmonic nanostructures.Moreover,due to the parallel arrangement of flow paths,simultaneous detection of multiple cancer biomarkers,including prostate-specific antigen and carcinoembryonic antigen,has been fulfilled with increased signal-to-background ratios.Given the high performance of this assay,together with its simple fabrication process that is compatible with standard mass manufacturing techniques,we expect that the prepared integrated nanorod device can bring on-site point-of-care diagnosis closer to reality.展开更多
基金STI2030-Major Projects(2022ZD0204600)National Natural Science Foundation of China(32100805,82070997)CAS Project for Young Scientists in Basic Research(YSBR-113)。
文摘Long-term exposure to monochromatic light during early life has been shown to significantly impact the development of myopia.However,its effect on color processing in the visual cortex remains unclear.In this study,we investigated the effects of different lighting conditions on the functional organization of color representation in the visual cortical areas V1,V2,and V4 of rhesus monkeys raised under long-wave and short-wave monochromatic illumination for four years.Using cytochrome oxidase staining and intrinsic signal optical imaging,we found that the sizes,densities,and response strengths of cortical color domains in V1,V2,and V4 were consistent across illumination conditions.In addition,the cortical distances between specific hue response patches did not significantly differ among the groups.These findings suggested that long-term monochromatic illumination does not alter the spatial organization or functional properties of color domains in the visual cortex of rhesus monkeys.This research provides new insights into the resilience of the visual system's chromatic representation despite altered lighting conditions in early life.
基金supported by the National Natural Science Foundation of China(Grant No.61701438)Shanghai Science and Technology Commission’s Scientific and Technological Innovation Action Plan(No.19495810300)+2 种基金Fundamental Research Funds for the Central Universities,China(Grant No.2020XZZX002-13)Zhejiang Province Key R&D programs(Nos.2020C03039,2020C01120,2021C03062,and 2021C03108)Science and Technology Service Network Initiative(STS)of the Chinese Academy of Sciences(No.KFJ-STS-QYZX-061).
文摘Here,we present integrated nanorod arrays on microfluidic chips for fast and sensitive flow-through immunoassays of physiologically relevant macromolecules.Dense arrays of Au nanorods are easily fabricated through one-step oblique angle deposition,which eliminates the requirement of advanced lithography methods.We report the utility of this plasmonic structure to improve the detection limit of the cardiac troponin I(cTnI)assay by over 6x 105-fold,reaching down to 33.9fg mL^(-1)(-1.4fM) compared with an identical assay on glass substrates.Through monolithic integration with microfluidic elements,the device enables a flow-through assay for quantitative detection of cTnI in the serum with a detection sensitivity of 6.9 pg mL^(-1)(-0.3 pM)in<6 min,which was 4000 times lower than conventional glass devices.This ultrasensitive detection arises from the large surface area for antibody conjugation and metal-enhanced fluorescent signals through plasmonic nanostructures.Moreover,due to the parallel arrangement of flow paths,simultaneous detection of multiple cancer biomarkers,including prostate-specific antigen and carcinoembryonic antigen,has been fulfilled with increased signal-to-background ratios.Given the high performance of this assay,together with its simple fabrication process that is compatible with standard mass manufacturing techniques,we expect that the prepared integrated nanorod device can bring on-site point-of-care diagnosis closer to reality.
