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洛伐他汀工业菌株土曲霉HZ01的次级代谢产物合成分析 被引量:5
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作者 韦苏慧 黄雪年 +3 位作者 张伟 路尧 韩力挥 吕雪峰 《微生物学报》 CAS CSCD 北大核心 2018年第5期793-803,共11页
【目的】分析洛伐他汀工业生产菌株土曲霉HZ01的次级代谢产物合成能力,为后期的遗传改造、次级代谢产物及其基因簇挖掘提供指导。【方法】对洛伐他汀发酵条件下的样品进行了转录组分析,同时运用色谱分离技术及波谱学方法对主要次级代谢... 【目的】分析洛伐他汀工业生产菌株土曲霉HZ01的次级代谢产物合成能力,为后期的遗传改造、次级代谢产物及其基因簇挖掘提供指导。【方法】对洛伐他汀发酵条件下的样品进行了转录组分析,同时运用色谱分离技术及波谱学方法对主要次级代谢产物进行了分离和结构鉴定。【结果】洛伐他汀合成相关基因转录水平非常高,还有4个聚酮合酶(PKS)、6个非核糖体多肽合成酶(NRPS)和1个PKS-NRPS杂合酶基因进行了转录,其他PKS和NRPS基因都处于沉默状态。此外,从该菌的发酵产物中分离鉴定了10个主要副产物并确定了其结构。【结论】土曲霉HZ01是一株优良的洛伐他汀生产菌株,在构建次级代谢产物异源合成细胞工厂和鉴定次级代谢产物生物合成途径方面具有很好的应用潜力。 展开更多
关键词 土曲霉 次级代谢产物 洛伐他汀 工业菌株 转录组
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Temporal-spatial distributions of road silt loadings and fugitive road dust emissions in Beijing from 2019 to 2020 被引量:2
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作者 Haibin Wang lihui han +6 位作者 Tingting Li Song Qu Yuncheng Zhao Shoubin Fan Tong Chen Haoran Cui Junfang Liu 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2023年第10期56-70,共15页
Road silt loading(sL)is an important parameter in the fugitive road dust(FRD)emissions.In this study,the improved Testing Re-entrained Aerosol Kinetic Emissions from Roads(TRAKER)combined with the AP-42 method was fir... Road silt loading(sL)is an important parameter in the fugitive road dust(FRD)emissions.In this study,the improved Testing Re-entrained Aerosol Kinetic Emissions from Roads(TRAKER)combined with the AP-42 method was firstly developed to quickly measure and estimate the sLs of paved roads in Beijing,China.The annual average sLs in Beijing was 0.59±0.31 g/m^(2)in 2020,and decreased by 22.4%compared with that in 2019.The seasonal variations of sLs followed the order of spring>winter>summer>autumn in the two years.The seasonal mean road sLs on the same type road in the four seasons presented a decline trend from^(2)019 to 2020,especially on the Expressway,decreasing 47.4%-72.7%.The road sLs on the different type roads in the same season followed the order of Major arterial∼Minor arterial∼Branch road>Express road,and Township road∼Country highway>Provincial highway∼National highway.The emission intensities of PM10 and PM_(2.5)from FRD in Beijing in 2020 were lower than those in 2019.The PM10 and PM_(2.5)emission intensities at the four planning areas in the two years all presented the order of the capital functional core area>the urban functional expansion area>the urban development new area>the ecological conservation and development area.The annual emissions of PM10 and PM_(2.5)from FRD in Beijing in 2020 were 74,886 ton and 18,118 ton,respectively,decreasing by∼33.3%compared with those in 2019. 展开更多
关键词 Road silt loading Temporal-spatial distribution Emission factor Emission inventory
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Diversity of New Three-Wave Solutions and New Periodic Waves for the (3 + 1)-Dimensional Kadomtsev-Petviashvili-Boussinesq-Like Equation 被引量:1
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作者 Meiyu Li Sudao Bilige +1 位作者 Runfa Zhang lihui han 《Journal of Applied Mathematics and Physics》 2020年第10期2142-2156,共15页
Based on the generalized bilinear method, diversity of exact solutions of the (3 + 1)-dimensional Kadomtsev-Petviashvili-Boussinesq-like equation is successfully derived by using symbolic computation with Maple. These... Based on the generalized bilinear method, diversity of exact solutions of the (3 + 1)-dimensional Kadomtsev-Petviashvili-Boussinesq-like equation is successfully derived by using symbolic computation with Maple. These new solutions, named three-wave solutions and periodic wave have greatly enriched the existing literature. Via the three-dimensional images, density images and contour plots, the physical characteristics of these waves are well described. The new three-wave solutions and periodic solitary wave solutions obtained in this paper, will have a wide range of applications in the fields of physics and mechanics. 展开更多
关键词 KPB-Like Equation Generalized Bilinear Form New Three-Wave Solutions New Periodic Wave
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