The contamination of pork during breeding can negatively impact both food quality and safety.Sodium dichloroiso-cyanurate(NaDCC),a chlorine-containing disinfectant,is widely used in animal environments.In this study,1...The contamination of pork during breeding can negatively impact both food quality and safety.Sodium dichloroiso-cyanurate(NaDCC),a chlorine-containing disinfectant,is widely used in animal environments.In this study,16S rRNA sequencing was used to determine the bacterial communities in a pork farm.We also assessed the effectiveness of NaDCC disinfection by analyzing microbial diversity.The results revealed that the predominant genera in pork skin samples and environmental samples were Staphylococcus and Acinetobacter,respectively.Escherichia coli and Strep-tococcus equinus were present in all the samples,suggesting that NaDCC is not fully effective in preventing zoonotic pathogen contamination.The Chao1 and Shannon indices for sow skins increased after disinfection.No significant differences were observed in the microbiological composition of sow barn samples before and after disinfection(p>0.1).Additionally,bacterial abundance in sow skin was strongly correlated with abundance on pen floors(r=0.906,p<0.05),indicating potential cross-contamination between these sample types.Conversely,the bacterial abun-dance in the floor samples was weakly correlated with that in the other samples,suggesting that NaDCC is effective as a decontaminant.This study provides valuable insights into microbial contamination on pork farms and under-scores the importance of proper disinfection practices.This study also offers scientific recommendations for improv-ing disinfection protocols.展开更多
基金supported by the Natural Science Foundation of China(32302233,32472466)the Natural Science Foundation of Zhejiang(LZ24C200004)+6 种基金the Key Research and Development Program of Zhejiang Province(2022C02049)the Key Research and Development Program of Ningbo(2022Z178)the Ministry of Agriculture and Rural Affairs(20244027)the WalMart Foundation(UA2020-152,UA2021-247)the State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(2021DG700024-KF202517)the Zhejiang Provincial Department of Agriculture and Rural Affairs Project(2024SNJF044)the Key Research and Development Program of Zhuji(2022J10).
文摘The contamination of pork during breeding can negatively impact both food quality and safety.Sodium dichloroiso-cyanurate(NaDCC),a chlorine-containing disinfectant,is widely used in animal environments.In this study,16S rRNA sequencing was used to determine the bacterial communities in a pork farm.We also assessed the effectiveness of NaDCC disinfection by analyzing microbial diversity.The results revealed that the predominant genera in pork skin samples and environmental samples were Staphylococcus and Acinetobacter,respectively.Escherichia coli and Strep-tococcus equinus were present in all the samples,suggesting that NaDCC is not fully effective in preventing zoonotic pathogen contamination.The Chao1 and Shannon indices for sow skins increased after disinfection.No significant differences were observed in the microbiological composition of sow barn samples before and after disinfection(p>0.1).Additionally,bacterial abundance in sow skin was strongly correlated with abundance on pen floors(r=0.906,p<0.05),indicating potential cross-contamination between these sample types.Conversely,the bacterial abun-dance in the floor samples was weakly correlated with that in the other samples,suggesting that NaDCC is effective as a decontaminant.This study provides valuable insights into microbial contamination on pork farms and under-scores the importance of proper disinfection practices.This study also offers scientific recommendations for improv-ing disinfection protocols.
