Poly(phthalazinone ether sulfone ketone)(PPESK)is a new-generation high-performance thermoplastic resin that exhibits excellent thermal stability and mechanical properties.However,its damage and failure mechanisms und...Poly(phthalazinone ether sulfone ketone)(PPESK)is a new-generation high-performance thermoplastic resin that exhibits excellent thermal stability and mechanical properties.However,its damage and failure mechanisms under high-temperature and high-strain-rate coupling conditions remain unclear,significantly limiting the engineering applications of PPESK-based composites in extreme environments such as aerospace.To address this issue,in this study,a temperature-controlled split Hopkinson pressure bar experimental platform was developed for dynamic tensile/compressive loading scenarios.Combined with scanning electron microscopy and molecular dynamics simulations,the thermomechanical behavior and failure mechanisms of PPESK were systematically investigated over the temperature range of 293-473 K.The study revealed a novel"dynamic hysteresis brittle behavior"and its underlying"segmental activation±response lag antagonistic mechanism".The results showed that the strain-rate-induced response lag of polymer chain segments significantly weakened the viscous dissipation capacity activated by thermal energy at elevated temperatures.Although high-strain-rate conditions led to notable enhancement in the dynamic strength of the material(with an increase of 8%-233%,reaching 130%-330%at elevated temperatures),the fracture surface morphology tended to become smoother,and brittle fracture characteristics became more pronounced.Based on these findings,a temperature±strain rate hysteresis antagonistic function was constructed,which effectively captured the competitive relationship between temperature-driven relaxation behavior and strain-rateinduced hysteresis in thermoplastic resins.A multiscale damage evolution constitutive model with temperature±rate coupling was subsequently established and numerically implemented via the VUMAT user subroutine.This study not only unveils the nonlinear damage mechanisms of PPESK under combined service temperatures and dynamic/static loading conditions,but also provides a strong theoretical foundation and engineering guidance for the constitutive modeling and parametric design of thermoplastic resin-based materials.展开更多
Flexible microporous metal rubber(FMP-MR)is widely used in national defense applications,yet its mechanical behavior under high-speed impact conditions remains insufficiently explored.In this study,dynamic and static ...Flexible microporous metal rubber(FMP-MR)is widely used in national defense applications,yet its mechanical behavior under high-speed impact conditions remains insufficiently explored.In this study,dynamic and static experiments were conducted to systematically investigate the mechanical response of metal-wrapped microporous materials under impact loading that spanned 10~6 orders of magnitude.By combining a high-precision numerical model with a spatial contact point search algorithm,the spatio–temporal contact characteristics of the complex network structure in FMP-MR were systematically analyzed.Furthermore,the mapping mechanism from turn topology and mesoscopic friction behavior to macroscopic mechanical properties was comprehensively explored.The results showed that compared with quasi-static loading,FMP-MR under high-speed impact exhibited higher energy absorption efficiency due to high-strain-rate inertia effect.Therefore,the peak stress increased by 141%,and the maximum energy dissipation increased by 300%.Consequently,the theory of dynamic friction locking effect was innovatively proposed.The theory explains that the close synergistic effect of sliding friction and plastic dissipation promoted by the stable interturn-locked embedded structure is the essential reason for the excellent dynamic mechanical properties of FMP-MR under dynamic loading conditions.Briefly,based on the in-depth investigation of the mechanical response and energy dissipation mechanism of FMP-MR under impact loads,this study provides a solid theoretical basis for further expanding the application range of FMP-MR and optimizing its performance.展开更多
Posterior capsular opacification(PCO),the most common complication after cataract surgery,is caused by the proliferation,migration and differentiation of residual lens epithelial cells(LECs)on the surface of the intra...Posterior capsular opacification(PCO),the most common complication after cataract surgery,is caused by the proliferation,migration and differentiation of residual lens epithelial cells(LECs)on the surface of the intraocular lens(IOL).Although drug-loaded IOLs have been successfully developed,the PCO prevention efficacy is still limited due to the lack of targeting and low bioavailability.In this investigation,an exosome-functionalized drug-loaded IOL was successfully developed for effective PCO prevention utilizing the homologous targeting and high biocompatibility of exosome.The exosomes derived from LECs were collected to load the anti-proliferative drug doxorubicin(Dox)through electroporation and then immobilized on the aminated IOLs surface through electrostatic interaction.In vitro experiments showed that significantly improved cellular uptake of Dox@Exos by LECs was achieved due to the targeting ability of exosome,compared with free Dox,thus resulting in superior anti-proliferation effect.In vivo animal investigations indicated that Dox@Exos-IOLs effectively inhibited the development of PCO and showed excellent intraocular biocompatibility.We believe that this work will provide a targeting strategy for PCO prevention through exosome-functionalized IOL.展开更多
Photosystem I(PSI)is a large protein supercomplex that catalyzes the light-dependent oxidation of plastocyanin(or cytochrome c6)and the reduction of ferredoxin.This catalytic reaction is realized by a transmembrane el...Photosystem I(PSI)is a large protein supercomplex that catalyzes the light-dependent oxidation of plastocyanin(or cytochrome c6)and the reduction of ferredoxin.This catalytic reaction is realized by a transmembrane electron transfer chain consisting of primary electron donor(a special chlorophyll(Chl)pair)and electron acceptors A_(0),A_(1),and three Fe_(4)S_(4) clusters,F_(X),F_(A),and F_(B).Here we report the PSI structure from a Chl d-dominated cyanobacterium Acaryochloris marina at 3.3Åresolution obtained by single-particle cryo-electron microscopy.The A.marina PSI exists as a trimer with three identical monomers.Surprisingly,the structure reveals a unique composition of electron transfer chain in which the primary electron acceptor A_(0) is composed of two pheophytin a rather than Chl a found in any other well-known PSI structures.A novel subunit Psa27 is observed in the A.marina PSI structure.In addition,77 Chls,13α-carotenes,two phylloquinones,three Fe-S clusters,two phosphatidyl glycerols,and one monogalactosyl-diglyceride were identified in each PSI monomer.Our results provide a structural basis for deciphering the mechanism of photosynthesis in a PSI complex with Chl d as the dominating pigments and absorbing far-red light.展开更多
Dry eye disease(DED)is the most common eye disease in ophthalmic consultation except for refractive errors.Therefore,an exploration of valid and alternative therapeutic interventions is essential to feed the urgent me...Dry eye disease(DED)is the most common eye disease in ophthalmic consultation except for refractive errors.Therefore,an exploration of valid and alternative therapeutic interventions is essential to feed the urgent medical need.It has been demonstrated that oxidative stress causes multiple adverse effects in the pathogenesis of DED,thence alleviating oxidative stress is an effective therapeutic strategy for the DED treatment.Herein,we developed a cerium oxide nanozyme combined with branched poly(ethylene imine)-graftpoly(ethylene glycol)(bPEI-g-PEG).Owing to its stable hydrophilic chains on the surface reducing the cytotoxicity and loads of amines groups that be combined with cerium ions through coordination bonds,the modified nanozymes(referred to as CNP@bPEI-g-PEG)are water soluble and highly biocompatible.Meanwhile,due to its excellent antioxidant activity,CNP@bPEI-g-PEG nanozymes can mimic the activity of superoxide dismutase and catalase to scavenge intracellular reactive oxygen species(ROS).Experimental studies firmly demonstrated that the modified nanozymes were auto-regenerative and more active in scavenging excessive ROS and alleviating oxidative stress by cerium-element valence state recycling,recovering the morphology of corneal,conjunctival epithelium and the number of goblet cells.The advanced combination may offer a superior therapeutic strategy to deal with oxidative stress for effective treatment of DED.展开更多
Flexible microporous metal rubber (FMP-MR) is a high-damping material that dissipates energy by dry friction through internal spiral metal wires in contact with each other. However, the FMP-MR energy dissipation mecha...Flexible microporous metal rubber (FMP-MR) is a high-damping material that dissipates energy by dry friction through internal spiral metal wires in contact with each other. However, the FMP-MR energy dissipation mechanism is not fully understood owing to its disordered grid interpenetrating structure. In this work, computer-aided preparation technology is used to accurately reconstruct the complex spiral network structure of FMP-MR multipoint random contact, and a cell group model with an energy dissipation mechanism is proposed to obtain the dynamic energy distribution of the contact friction in both space and time dimensions. By judging the effective contact point, a global displacement ablation phenomenon of hooked staggered porous materials is induced. The macro- and micro-equivalent frictions are introduced to effectively explain the characteristics of the strong energy dissipation in FMP-MR under fretting excitation. A real and effective damping hysteresis constitutive model is then constructed to dynamically capture the mapping relationship between the complex nonlinear topological structure effect of the materials and spatial random contact dry friction in real time. The results indicate that the contact behavior between turns of the FMP-MR wire follows a clear quasi-Gaussian distribution under an external load, forcing the topological results to change. The energy dissipation of the materials revealed peak energy consumption lagging behind the loading limit for a certain distance, which can be determined by the effective contact point and contact dry friction slip. The consistency between the quasi-static compression tests and constitutive curves of the model was quantitatively verified through residual analysis. The data demonstrated the differential behavior of the FMP-MR meso-structure to follow a phased growth law during loading with different action mechanisms in the guiding, main growth, and relaxation stages of the energy consumption displacement curve. In summary, these findings provide an acceptable theoretical basis for the damping energy consumption mechanism and lifetime prediction of FMP-MR.展开更多
Silicon carbide fiber reinforced silicon carbide matrix(SiC_(f)/SiC)composite is the key cladding material of nuclear fuel,which determines the safety and reliability of nuclear fuel storage and transportation.The rep...Silicon carbide fiber reinforced silicon carbide matrix(SiC_(f)/SiC)composite is the key cladding material of nuclear fuel,which determines the safety and reliability of nuclear fuel storage and transportation.The replacement of its storage and transportation scenario needs to be completed by the manipulator,but the application of SiC_(f)/SiC wear,fracture,and nuclear leakage in the snatching process of brittle-flexible-rigid contact in the irradiation environment has been seriously restricted due to unclear understanding of the damage mechanism.Therefore,the effects of irradiation dose and clamping load on the friction characteristics of the contact interface between SiC_(f)/SiC clad tube are studied in this paper,and the effects of irradiation parameters and clamping force on the static friction coefficient of the contact interface between the clad tube and flexible nitrile are obtained.Based on the Greenwood-Williamson tribological model,a numerical model of the shape and structure of the contact micro-convex at the micro-scale of the clamping interface is constructed by introducing the multi-surface integral,and finally verified by experiments.The research results show that there is a unique“Irradiation suppression zone”under the clamping condition of SiC_(f)/SiC cladding tube under the nuclear irradiation environment,and the growth of static friction coefficient slows down until stagnates after irradiation reaches a certain extent(600 kGy),and there will be a decline when the irradiation dose continues to increase,among which the clamping force of 15.2 N within the irradiation interval of 1,000 kGy can meet the safety of nuclear environment operation.The results of this paper can provide an important theoretical basis and application guidance for the safe operation of SiC_(f)/SiC cladding tubes in the storage and transportation clamping process.展开更多
A complex micelle as a hemoglobin functional model with the biaoactive function of reversible oxygen transfer has been constructed through the hierarchical assembly of the diblock copolymer poly（ethylene glycol）-blo...A complex micelle as a hemoglobin functional model with the biaoactive function of reversible oxygen transfer has been constructed through the hierarchical assembly of the diblock copolymer poly（ethylene glycol）-block- poly（4-vinylpyridine-co-N-heptyl-4-vinylpyridine） （PEG-b-P（4VP-co-4VPHep））, tetrakis（4-sulfonatophenyl）porphinato iron（II） （Fe（II）TPPS） and β-cyclodextrin （β-CD）. The μ-oxo dimer of Fe（II）TPPS was successfully inhibited because the Fe（II）TPPS was included into the cavities of β-CDs through host-vip interaction. Fe（II）TPPS coordinated with pyridine groups functions as the active site to reversibly bind dioxygen. In adition, the host-vip inclusion （β-CD/Fe（II）TPPS） was encapsulated in the hydrophobic core of the complex micelle and tightly fixed by P4VP chains. The hydrophilic PEG blocks stretched in aqueous solution to constitute the shells which stabilize the structure of the complex micelle as well as endow the complex micelle with sufficient blood circulation time. Dioxygen can be bound to the Fe（II）TPPS located in the confined space and excellent reversibility of the binding-release process of dioxygen can be achieved. The quaternary amine N-heptyl-4-vinylpyridine can coerce abundant S2O4^2- ions into the core of the complex micelle to facilitate the self-reduction process. Dioxygen adducts （Fe（II）TPPS（O2）） were effectively protected by the double hydrophobic barriers constructed by the cavity of the cyclodextrin and the core of the complex micelle which enhances the ability to resist nucleophilic molecules. Therefore, the rationally designed amphiphilic structure can work as a promising artificial O2 carrier. Potentially, the complex micelle can be expected to improve the treatment of diseases linked with hypoxia.展开更多
基金supported by National Key Research and Development Program"Advanced Structures and Composite Materials"Special Project[Grant No.2024YFB3712800]the Fundamental Research Funds for the Central Universities[Grant No.DUT22-LAB605]Liaoning Province's"Unveiling the List and Leading the Way"Science and Technology Research and Development Special Project[Grant No.2022JH1/10400043]。
文摘Poly(phthalazinone ether sulfone ketone)(PPESK)is a new-generation high-performance thermoplastic resin that exhibits excellent thermal stability and mechanical properties.However,its damage and failure mechanisms under high-temperature and high-strain-rate coupling conditions remain unclear,significantly limiting the engineering applications of PPESK-based composites in extreme environments such as aerospace.To address this issue,in this study,a temperature-controlled split Hopkinson pressure bar experimental platform was developed for dynamic tensile/compressive loading scenarios.Combined with scanning electron microscopy and molecular dynamics simulations,the thermomechanical behavior and failure mechanisms of PPESK were systematically investigated over the temperature range of 293-473 K.The study revealed a novel"dynamic hysteresis brittle behavior"and its underlying"segmental activation±response lag antagonistic mechanism".The results showed that the strain-rate-induced response lag of polymer chain segments significantly weakened the viscous dissipation capacity activated by thermal energy at elevated temperatures.Although high-strain-rate conditions led to notable enhancement in the dynamic strength of the material(with an increase of 8%-233%,reaching 130%-330%at elevated temperatures),the fracture surface morphology tended to become smoother,and brittle fracture characteristics became more pronounced.Based on these findings,a temperature±strain rate hysteresis antagonistic function was constructed,which effectively captured the competitive relationship between temperature-driven relaxation behavior and strain-rateinduced hysteresis in thermoplastic resins.A multiscale damage evolution constitutive model with temperature±rate coupling was subsequently established and numerically implemented via the VUMAT user subroutine.This study not only unveils the nonlinear damage mechanisms of PPESK under combined service temperatures and dynamic/static loading conditions,but also provides a strong theoretical foundation and engineering guidance for the constitutive modeling and parametric design of thermoplastic resin-based materials.
基金National Natural Science Foundation of China-NSAF(Grant No.U2330202)the National Natural Science Foundation of China(Grant Nos.52175162 and 51805086)+1 种基金Fujian Provincial Technological Innovation Key Research and Industrialization Projects(Grant Nos.2023XQ005 and 2024XQ010)The National Independent Innovation Demonstration Platform Project of Fujian Province(2024QZFX07)。
文摘Flexible microporous metal rubber(FMP-MR)is widely used in national defense applications,yet its mechanical behavior under high-speed impact conditions remains insufficiently explored.In this study,dynamic and static experiments were conducted to systematically investigate the mechanical response of metal-wrapped microporous materials under impact loading that spanned 10~6 orders of magnitude.By combining a high-precision numerical model with a spatial contact point search algorithm,the spatio–temporal contact characteristics of the complex network structure in FMP-MR were systematically analyzed.Furthermore,the mapping mechanism from turn topology and mesoscopic friction behavior to macroscopic mechanical properties was comprehensively explored.The results showed that compared with quasi-static loading,FMP-MR under high-speed impact exhibited higher energy absorption efficiency due to high-strain-rate inertia effect.Therefore,the peak stress increased by 141%,and the maximum energy dissipation increased by 300%.Consequently,the theory of dynamic friction locking effect was innovatively proposed.The theory explains that the close synergistic effect of sliding friction and plastic dissipation promoted by the stable interturn-locked embedded structure is the essential reason for the excellent dynamic mechanical properties of FMP-MR under dynamic loading conditions.Briefly,based on the in-depth investigation of the mechanical response and energy dissipation mechanism of FMP-MR under impact loads,this study provides a solid theoretical basis for further expanding the application range of FMP-MR and optimizing its performance.
基金supported by the National Key Research and Development Program of China(2017YFC1104602)the Key Scientific and Technological Innovation Projects in Wenzhou(ZY2021002)+2 种基金the Medical&Health Technology Program of Zhejiang Province(2022RC051)the Zhejiang Science and Technology Program of Traditional Chinese Medicine(2022ZB220)Science&Technology Program of Wenzhou(Y2020204).
文摘Posterior capsular opacification(PCO),the most common complication after cataract surgery,is caused by the proliferation,migration and differentiation of residual lens epithelial cells(LECs)on the surface of the intraocular lens(IOL).Although drug-loaded IOLs have been successfully developed,the PCO prevention efficacy is still limited due to the lack of targeting and low bioavailability.In this investigation,an exosome-functionalized drug-loaded IOL was successfully developed for effective PCO prevention utilizing the homologous targeting and high biocompatibility of exosome.The exosomes derived from LECs were collected to load the anti-proliferative drug doxorubicin(Dox)through electroporation and then immobilized on the aminated IOLs surface through electrostatic interaction.In vitro experiments showed that significantly improved cellular uptake of Dox@Exos by LECs was achieved due to the targeting ability of exosome,compared with free Dox,thus resulting in superior anti-proliferation effect.In vivo animal investigations indicated that Dox@Exos-IOLs effectively inhibited the development of PCO and showed excellent intraocular biocompatibility.We believe that this work will provide a targeting strategy for PCO prevention through exosome-functionalized IOL.
基金The project was funded by the National Key R&D Program of China(2020YFA0907600,2017YFA0503700,2017YFA0504803,2018YFA0507700,2019YFA0906300)the Strategic Priority Research Program of CAS(XDA27050402,XDB17000000)+2 种基金the Chinese Academy of Sciences Key Research Program of Frontier Sciences(QYZDY-SSW-SMC003)Youth Innovation Promotion Association of CAS(2020081),CAS Interdisciplinary Innovation Team(JCTD-2020-06)the Fundamental Research Funds for the Central Universities(2018XZZX001-13).
文摘Photosystem I(PSI)is a large protein supercomplex that catalyzes the light-dependent oxidation of plastocyanin(or cytochrome c6)and the reduction of ferredoxin.This catalytic reaction is realized by a transmembrane electron transfer chain consisting of primary electron donor(a special chlorophyll(Chl)pair)and electron acceptors A_(0),A_(1),and three Fe_(4)S_(4) clusters,F_(X),F_(A),and F_(B).Here we report the PSI structure from a Chl d-dominated cyanobacterium Acaryochloris marina at 3.3Åresolution obtained by single-particle cryo-electron microscopy.The A.marina PSI exists as a trimer with three identical monomers.Surprisingly,the structure reveals a unique composition of electron transfer chain in which the primary electron acceptor A_(0) is composed of two pheophytin a rather than Chl a found in any other well-known PSI structures.A novel subunit Psa27 is observed in the A.marina PSI structure.In addition,77 Chls,13α-carotenes,two phylloquinones,three Fe-S clusters,two phosphatidyl glycerols,and one monogalactosyl-diglyceride were identified in each PSI monomer.Our results provide a structural basis for deciphering the mechanism of photosynthesis in a PSI complex with Chl d as the dominating pigments and absorbing far-red light.
基金financially supported by the Key Scientific and Technological Innovation Projects in Wenzhou[ZY2021002]the Medical&Health Technology Program of Zhejiang Province[2022RC051].
文摘Dry eye disease(DED)is the most common eye disease in ophthalmic consultation except for refractive errors.Therefore,an exploration of valid and alternative therapeutic interventions is essential to feed the urgent medical need.It has been demonstrated that oxidative stress causes multiple adverse effects in the pathogenesis of DED,thence alleviating oxidative stress is an effective therapeutic strategy for the DED treatment.Herein,we developed a cerium oxide nanozyme combined with branched poly(ethylene imine)-graftpoly(ethylene glycol)(bPEI-g-PEG).Owing to its stable hydrophilic chains on the surface reducing the cytotoxicity and loads of amines groups that be combined with cerium ions through coordination bonds,the modified nanozymes(referred to as CNP@bPEI-g-PEG)are water soluble and highly biocompatible.Meanwhile,due to its excellent antioxidant activity,CNP@bPEI-g-PEG nanozymes can mimic the activity of superoxide dismutase and catalase to scavenge intracellular reactive oxygen species(ROS).Experimental studies firmly demonstrated that the modified nanozymes were auto-regenerative and more active in scavenging excessive ROS and alleviating oxidative stress by cerium-element valence state recycling,recovering the morphology of corneal,conjunctival epithelium and the number of goblet cells.The advanced combination may offer a superior therapeutic strategy to deal with oxidative stress for effective treatment of DED.
基金the National Natural Science Foundation of China(Grant Nos.52175162,51805086,and 51975123)the Natural Science Foundation of Fujian Province(Grant No.2019J01210)Health education joint project of Fujian Province(Grant No.2019-WJ-01).
文摘Flexible microporous metal rubber (FMP-MR) is a high-damping material that dissipates energy by dry friction through internal spiral metal wires in contact with each other. However, the FMP-MR energy dissipation mechanism is not fully understood owing to its disordered grid interpenetrating structure. In this work, computer-aided preparation technology is used to accurately reconstruct the complex spiral network structure of FMP-MR multipoint random contact, and a cell group model with an energy dissipation mechanism is proposed to obtain the dynamic energy distribution of the contact friction in both space and time dimensions. By judging the effective contact point, a global displacement ablation phenomenon of hooked staggered porous materials is induced. The macro- and micro-equivalent frictions are introduced to effectively explain the characteristics of the strong energy dissipation in FMP-MR under fretting excitation. A real and effective damping hysteresis constitutive model is then constructed to dynamically capture the mapping relationship between the complex nonlinear topological structure effect of the materials and spatial random contact dry friction in real time. The results indicate that the contact behavior between turns of the FMP-MR wire follows a clear quasi-Gaussian distribution under an external load, forcing the topological results to change. The energy dissipation of the materials revealed peak energy consumption lagging behind the loading limit for a certain distance, which can be determined by the effective contact point and contact dry friction slip. The consistency between the quasi-static compression tests and constitutive curves of the model was quantitatively verified through residual analysis. The data demonstrated the differential behavior of the FMP-MR meso-structure to follow a phased growth law during loading with different action mechanisms in the guiding, main growth, and relaxation stages of the energy consumption displacement curve. In summary, these findings provide an acceptable theoretical basis for the damping energy consumption mechanism and lifetime prediction of FMP-MR.
基金supported by the National Natural Science Foundation of China(Grant No.52075526)the“Ningbo 3315 Plan Innovation Team”(Grant No.2017A-28-C)+2 种基金the National Natural Science Foundation of China(Grant No.91860204)the Fundamental Research Funds for the Central Universities(Grant No.DUT22LAB605)the National Key R&D Program of China(Grant No.2018YFB1107500).
文摘Silicon carbide fiber reinforced silicon carbide matrix(SiC_(f)/SiC)composite is the key cladding material of nuclear fuel,which determines the safety and reliability of nuclear fuel storage and transportation.The replacement of its storage and transportation scenario needs to be completed by the manipulator,but the application of SiC_(f)/SiC wear,fracture,and nuclear leakage in the snatching process of brittle-flexible-rigid contact in the irradiation environment has been seriously restricted due to unclear understanding of the damage mechanism.Therefore,the effects of irradiation dose and clamping load on the friction characteristics of the contact interface between SiC_(f)/SiC clad tube are studied in this paper,and the effects of irradiation parameters and clamping force on the static friction coefficient of the contact interface between the clad tube and flexible nitrile are obtained.Based on the Greenwood-Williamson tribological model,a numerical model of the shape and structure of the contact micro-convex at the micro-scale of the clamping interface is constructed by introducing the multi-surface integral,and finally verified by experiments.The research results show that there is a unique“Irradiation suppression zone”under the clamping condition of SiC_(f)/SiC cladding tube under the nuclear irradiation environment,and the growth of static friction coefficient slows down until stagnates after irradiation reaches a certain extent(600 kGy),and there will be a decline when the irradiation dose continues to increase,among which the clamping force of 15.2 N within the irradiation interval of 1,000 kGy can meet the safety of nuclear environment operation.The results of this paper can provide an important theoretical basis and application guidance for the safe operation of SiC_(f)/SiC cladding tubes in the storage and transportation clamping process.
文摘A complex micelle as a hemoglobin functional model with the biaoactive function of reversible oxygen transfer has been constructed through the hierarchical assembly of the diblock copolymer poly（ethylene glycol）-block- poly（4-vinylpyridine-co-N-heptyl-4-vinylpyridine） （PEG-b-P（4VP-co-4VPHep））, tetrakis（4-sulfonatophenyl）porphinato iron（II） （Fe（II）TPPS） and β-cyclodextrin （β-CD）. The μ-oxo dimer of Fe（II）TPPS was successfully inhibited because the Fe（II）TPPS was included into the cavities of β-CDs through host-vip interaction. Fe（II）TPPS coordinated with pyridine groups functions as the active site to reversibly bind dioxygen. In adition, the host-vip inclusion （β-CD/Fe（II）TPPS） was encapsulated in the hydrophobic core of the complex micelle and tightly fixed by P4VP chains. The hydrophilic PEG blocks stretched in aqueous solution to constitute the shells which stabilize the structure of the complex micelle as well as endow the complex micelle with sufficient blood circulation time. Dioxygen can be bound to the Fe（II）TPPS located in the confined space and excellent reversibility of the binding-release process of dioxygen can be achieved. The quaternary amine N-heptyl-4-vinylpyridine can coerce abundant S2O4^2- ions into the core of the complex micelle to facilitate the self-reduction process. Dioxygen adducts （Fe（II）TPPS（O2）） were effectively protected by the double hydrophobic barriers constructed by the cavity of the cyclodextrin and the core of the complex micelle which enhances the ability to resist nucleophilic molecules. Therefore, the rationally designed amphiphilic structure can work as a promising artificial O2 carrier. Potentially, the complex micelle can be expected to improve the treatment of diseases linked with hypoxia.
