Juglans sigillata is an economically valuable nut crop renowned for its nutritional richness,including essential nutrients,antioxidants,and healthy fats,which boost human cardial,brain and gut health.Despite its impor...Juglans sigillata is an economically valuable nut crop renowned for its nutritional richness,including essential nutrients,antioxidants,and healthy fats,which boost human cardial,brain and gut health.Despite its importance,the lack of a complete genome assembly has been a stumbling block in its biological breeding process.Therefore,we generated deep coverage ultralong Oxford Nanopore Technology(ONT)and PacBio HiFi reads to construct a telomere-to-telomere(T2T)genome assembly.The final assembly spans 537.27 Mb with no gaps,demonstrating a remarkable completeness of 98.1%.We utilized a combination of transcriptome data and homologous proteins to annotate the genome,identifying 36018 protein-coding genes.Furthermore,we profiled global cytosine DNA methylations using ONT sequencing data.Global methylome analysis revealed high methylation levels in transposable element(TE)-rich chromosomal regions juxtaposed with comparatively lower methylation in gene-rich areas.By integrating a detailed multi-omics data analysis,we obtained valuable insights into the mechanism underlying endopleura coloration.This investigation led to the identification of eight candidate genes(e.g.ANR)involved in anthocyanin biosynthesis pathways,which are crucial for the development of color in plants.The comprehensive genome assembly and the understanding of the genetic basis of important traits like endopleura coloration will open avenues for more efficient breeding programs and improved crop quality.展开更多
Using the data of 11 climatic factors on 40 counties in the main producing areas of walnut of Yunnan Province,we analyze the impact of various climatic factors on the distribution of Yunnan walnut.The results show tha...Using the data of 11 climatic factors on 40 counties in the main producing areas of walnut of Yunnan Province,we analyze the impact of various climatic factors on the distribution of Yunnan walnut.The results show that Yunnan walnut has a great expectation on temperature and moisture.Temperature,including average temperature in January,average temperature in July,the average annual temperature and accumulated temperature≥10℃,has the greatest impact on the distribution of Yunnan walnut,and is the primary dominant factor;moisture,including annual rainfall and average relative humidity,has a great impact on the distribution of Yunnan walnut,and is the secondary dominant factor.展开更多
基金supported by the Yunnan Seed Laboratory,China(202205AR070001-15)the National Natural Science Foundation of China,China(Grant No.32160697)。
文摘Juglans sigillata is an economically valuable nut crop renowned for its nutritional richness,including essential nutrients,antioxidants,and healthy fats,which boost human cardial,brain and gut health.Despite its importance,the lack of a complete genome assembly has been a stumbling block in its biological breeding process.Therefore,we generated deep coverage ultralong Oxford Nanopore Technology(ONT)and PacBio HiFi reads to construct a telomere-to-telomere(T2T)genome assembly.The final assembly spans 537.27 Mb with no gaps,demonstrating a remarkable completeness of 98.1%.We utilized a combination of transcriptome data and homologous proteins to annotate the genome,identifying 36018 protein-coding genes.Furthermore,we profiled global cytosine DNA methylations using ONT sequencing data.Global methylome analysis revealed high methylation levels in transposable element(TE)-rich chromosomal regions juxtaposed with comparatively lower methylation in gene-rich areas.By integrating a detailed multi-omics data analysis,we obtained valuable insights into the mechanism underlying endopleura coloration.This investigation led to the identification of eight candidate genes(e.g.ANR)involved in anthocyanin biosynthesis pathways,which are crucial for the development of color in plants.The comprehensive genome assembly and the understanding of the genetic basis of important traits like endopleura coloration will open avenues for more efficient breeding programs and improved crop quality.
基金Supported by National Science and Technology Support Program(2011BAD46B01-1)Yunnan Applied Basic Research Project(2012FD080)
文摘Using the data of 11 climatic factors on 40 counties in the main producing areas of walnut of Yunnan Province,we analyze the impact of various climatic factors on the distribution of Yunnan walnut.The results show that Yunnan walnut has a great expectation on temperature and moisture.Temperature,including average temperature in January,average temperature in July,the average annual temperature and accumulated temperature≥10℃,has the greatest impact on the distribution of Yunnan walnut,and is the primary dominant factor;moisture,including annual rainfall and average relative humidity,has a great impact on the distribution of Yunnan walnut,and is the secondary dominant factor.
