Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient co...Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient conditions to help plants cope with recurring environmental fluctuations.Despite the current understanding of plant circadian clock and genetic framework underlying plant shoot architecture,the intricate connection between these two adaptive mechanisms remains largely unclear.In this study,we elucidated how the core clock gene LUX ARRHYTHMO(LUX)regulates shoot architecture in the model legume plant Medicago truncatula.We show that mtlux mutant displays increased main stem height,reduced lateral shoot length,and decreased the number of lateral branches and biomass yield.Gene expression analysis revealed that Mt LUX regulated shoot architecture by repressing the expression of strigolactone receptor MtD14 and MtTB1/MtTCP1A,a TCP gene that functions centrally in modulating shoot architecture.In vivo and in vitro experiments showed that Mt LUX directly binds to a cis-element in the promoter of MtTB1/MtTCP1A,suggesting that Mt LUX regulates branching by rhythmically suppressing MtTB1/MtTCP1A.This work demonstrates the regulatory effect of the circadian clock on shoot architecture,offering a new understanding underlying the genetic basis towards the flexibility of plant shoot architecture.展开更多
Plants have a hierarchical circadian structure comprising multiple tissue-specific oscillators that operate at different speeds and regulate the expression of distinct sets of genes in different organs.However,the ide...Plants have a hierarchical circadian structure comprising multiple tissue-specific oscillators that operate at different speeds and regulate the expression of distinct sets of genes in different organs.However,the identity of the genes differentially regulated by the circadian clock in different organs,such as roots,and how their oscillations create functional specialization remain unclear.Here,we profiled the diurnal and circadian landscapes of the shoots and roots of Medicago truncatula and identified the conserved regulatory sequences contributing to transcriptome oscillations in each organ.We found that the light-dark cycles strongly affect the global transcriptome oscillation in roots,and many clock genes oscillate only in shoots.Moreover,many key genes involved in nitrogen fixation are regulated by circadian rhythms.Surprisingly,the root clock runs faster than the shoot clock,which is contrary to the hierarchical circadian structure showing a slow-paced root clock in both detached and intact Arabidopsis thaliana(L.)Heynh.roots.Our result provides important clues about the species-specific circadian regulatory mechanism,which is often overlooked,and possibly coordinates the timing between shoots and roots independent of the current prevailing model.展开更多
Soybean(Glycine max) produces seeds that are rich in unsaturated fatty acids and is an important oilseed crop worldwide. Seed oil content and composition largely determine the economic value of soybean. Due to natural...Soybean(Glycine max) produces seeds that are rich in unsaturated fatty acids and is an important oilseed crop worldwide. Seed oil content and composition largely determine the economic value of soybean. Due to natural genetic variation, seed oil content varies substantially across soybean cultivars. Although much progress has been made in elucidating the genetic trajectory underlying fatty acid metabolism and oil biosynthesis in plants, the causal genes for many quantitative trait loci(QTLs) regulating seed oil content in soybean remain to be revealed. In this study, we identified Gm FATA1B as the gene underlying a QTL that regulates seed oil content and composition, as well as seed size in soybean. Nine extra amino acids in the conserved region of Gm FATA1B impair its function as a fatty acyl–acyl carrier protein thioesterase, thereby affecting seed oil content and composition. Heterogeneously overexpressing the functional Gm FATA1B allele in Arabidopsis thaliana increased both the total oil content and the oleic acid and linoleic acid contents of seeds. Our findings uncover a previously unknown locus underlying variation in seed oil content in soybean and lay the foundation for improving seed oil content and composition in soybean.展开更多
Dear editor,Powdery mildew(PMD)is a widespread,fungal-borne disease that impacts crop yield worldwide.In soybean,PMD is caused by the fungal pathogen,Microsphaera diffusa.The most efficient and economic strategy for P...Dear editor,Powdery mildew(PMD)is a widespread,fungal-borne disease that impacts crop yield worldwide.In soybean,PMD is caused by the fungal pathogen,Microsphaera diffusa.The most efficient and economic strategy for PMD management with minimal environmental impact is through the deployment of resistance genes(Dangl et al.,2013;Hafeez et al.,2021).Although resistant genes against PMD have been identified in some crops,identification of those in soybean remains elusive.Several independent reports have consistently mapped the PMD-resistance locus to the end of Chr 16(Kang and Mian,2010;Jun et al.,2012;Jiang et al.,2019),however,the underlying gene that confers PMD resistance in soybean has yet to be cloned.Identification of the resistance-to-M.diffusa 1(Rmd1)gene is critical for the breeding of resistant soybean varieties,and thus control of PMD in this important crop.展开更多
基金supported by Laboratory of Lingnan Modern Agriculture Project(NZ2021001)State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources(SKICUSA-a202007)Natural Science Foundation of Guangdong Province(2022A1515011027,2019A1515012009)。
文摘Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient conditions to help plants cope with recurring environmental fluctuations.Despite the current understanding of plant circadian clock and genetic framework underlying plant shoot architecture,the intricate connection between these two adaptive mechanisms remains largely unclear.In this study,we elucidated how the core clock gene LUX ARRHYTHMO(LUX)regulates shoot architecture in the model legume plant Medicago truncatula.We show that mtlux mutant displays increased main stem height,reduced lateral shoot length,and decreased the number of lateral branches and biomass yield.Gene expression analysis revealed that Mt LUX regulated shoot architecture by repressing the expression of strigolactone receptor MtD14 and MtTB1/MtTCP1A,a TCP gene that functions centrally in modulating shoot architecture.In vivo and in vitro experiments showed that Mt LUX directly binds to a cis-element in the promoter of MtTB1/MtTCP1A,suggesting that Mt LUX regulates branching by rhythmically suppressing MtTB1/MtTCP1A.This work demonstrates the regulatory effect of the circadian clock on shoot architecture,offering a new understanding underlying the genetic basis towards the flexibility of plant shoot architecture.
基金Research in the laboratory of WH is supported by the National Natural Science Foundation of China(31700236)NSFC-Guangdong Joint Fund(U170120015)+1 种基金the Research Team Project from the Natural Science Foundation of Guangdong Province(2016A030312009)the Natural Science Foundation of Guangdong Province(2019A1515012009).
文摘Plants have a hierarchical circadian structure comprising multiple tissue-specific oscillators that operate at different speeds and regulate the expression of distinct sets of genes in different organs.However,the identity of the genes differentially regulated by the circadian clock in different organs,such as roots,and how their oscillations create functional specialization remain unclear.Here,we profiled the diurnal and circadian landscapes of the shoots and roots of Medicago truncatula and identified the conserved regulatory sequences contributing to transcriptome oscillations in each organ.We found that the light-dark cycles strongly affect the global transcriptome oscillation in roots,and many clock genes oscillate only in shoots.Moreover,many key genes involved in nitrogen fixation are regulated by circadian rhythms.Surprisingly,the root clock runs faster than the shoot clock,which is contrary to the hierarchical circadian structure showing a slow-paced root clock in both detached and intact Arabidopsis thaliana(L.)Heynh.roots.Our result provides important clues about the species-specific circadian regulatory mechanism,which is often overlooked,and possibly coordinates the timing between shoots and roots independent of the current prevailing model.
基金supported by the Seed Industry Revitalization Plan of Guangdong Province (2022-NPY-00-007)Key-Areas Research and Development Program of Guangdong Province (2022B0202060005)the China Agricultural Research System (CARS-04-PS 11)。
文摘Soybean(Glycine max) produces seeds that are rich in unsaturated fatty acids and is an important oilseed crop worldwide. Seed oil content and composition largely determine the economic value of soybean. Due to natural genetic variation, seed oil content varies substantially across soybean cultivars. Although much progress has been made in elucidating the genetic trajectory underlying fatty acid metabolism and oil biosynthesis in plants, the causal genes for many quantitative trait loci(QTLs) regulating seed oil content in soybean remain to be revealed. In this study, we identified Gm FATA1B as the gene underlying a QTL that regulates seed oil content and composition, as well as seed size in soybean. Nine extra amino acids in the conserved region of Gm FATA1B impair its function as a fatty acyl–acyl carrier protein thioesterase, thereby affecting seed oil content and composition. Heterogeneously overexpressing the functional Gm FATA1B allele in Arabidopsis thaliana increased both the total oil content and the oleic acid and linoleic acid contents of seeds. Our findings uncover a previously unknown locus underlying variation in seed oil content in soybean and lay the foundation for improving seed oil content and composition in soybean.
基金supported by the National Natural Science Foundation of China(31971966)the Key Area Research and Development Program of Guangdong Province(2020B020220008)the China Agricultural Research System(CARS-04-PS09),and Guangdong Laboratory for Lingnan Modern Agriculture.
文摘Dear editor,Powdery mildew(PMD)is a widespread,fungal-borne disease that impacts crop yield worldwide.In soybean,PMD is caused by the fungal pathogen,Microsphaera diffusa.The most efficient and economic strategy for PMD management with minimal environmental impact is through the deployment of resistance genes(Dangl et al.,2013;Hafeez et al.,2021).Although resistant genes against PMD have been identified in some crops,identification of those in soybean remains elusive.Several independent reports have consistently mapped the PMD-resistance locus to the end of Chr 16(Kang and Mian,2010;Jun et al.,2012;Jiang et al.,2019),however,the underlying gene that confers PMD resistance in soybean has yet to be cloned.Identification of the resistance-to-M.diffusa 1(Rmd1)gene is critical for the breeding of resistant soybean varieties,and thus control of PMD in this important crop.
