Dinitrobenzene(1,4-DNB) is one of the preferential toxicants stipulated by OECD and EPA of USA. With Oryzias latipes, which were chosen as the acceptors according to the international standard, the acute, subacute and...Dinitrobenzene(1,4-DNB) is one of the preferential toxicants stipulated by OECD and EPA of USA. With Oryzias latipes, which were chosen as the acceptors according to the international standard, the acute, subacute and accumulative toxicity experiments were conducted. The threshold mass concentration of O.latipes was 1.360 mg/L. The subacute experiment showed that O.latipes were hardly affected when the mass concentration of 1,4-DNB was 0.0292 mg/L, but were significantly affected when the mass concentration of 1,4-DNB was 0.0420, 0.0608 or 0.0875 mg/L in contrast to the control sample(P<0.05). The accumulative rate(K) was 1.21. This shows that there are significant accumulative actions of 1,4-DNB in fish. When the mass concentration of 1,4-DNB is in the range of 0.032_0.320 mg/L, the higher the 1,4-DNB concentration, the more strongly the Na +/K +-ATPase is inhibited. The calculated IC_ 50 values in gill, kidney and liver of O. latipes are 0.128, 0.132 and 0.428 mg/L, respectively.展开更多
In the whole power supply system, the transmission line is a very key link, and the transmission line determines whether the electricity can be transported smoothly, whether the power grid can operate safely and stabl...In the whole power supply system, the transmission line is a very key link, and the transmission line determines whether the electricity can be transported smoothly, whether the power grid can operate safely and stably. Power system transmission line project has the characteristics of long construction time, many participants, high quality risk and large investment, and has certain particularity compared with ordinary construction projects. This paper uses the investigation method, literature method and analysis method to focus on the transmission line management and quality control method for reference.展开更多
In the development process of state-owned enterprises, it is necessary to constantly improve the management system, pay attention to the improvement of human resource management system, so as to mobilize the enthusias...In the development process of state-owned enterprises, it is necessary to constantly improve the management system, pay attention to the improvement of human resource management system, so as to mobilize the enthusiasm of human resources, enhance the enthusiasm of personnel, and introduces more foreign high-quality foreign talents. With the continuous development of the market economy, the reform of state-owned enterprises should meet the corresponding requirements, actively change the management concept, encourage personnel ideological innovation, especially in the aspect of human resource management, strengthen the performance appraisal and salary management, optimize the personnel structure, and activate peoples ideological vitality.展开更多
In order to develop clinical diagnostic tools for rapid detection of SARS-CoV (severe acute respiratory syndrome-associated coronavirus) and to identify candidate proteins for vaccine development, the C-terminal porti...In order to develop clinical diagnostic tools for rapid detection of SARS-CoV (severe acute respiratory syndrome-associated coronavirus) and to identify candidate proteins for vaccine development, the C-terminal portion of the nucleocapsid (NC) gene was amplified using RT-PCR from the SARS-CoV genome, cloned into a yeast expression vector (pEGH), and expressed as a glutathione S-transferase (GST) and Hisx6 double-tagged fusion protein under the control of an inducible promoter. Western analysis on the purified protein confirmed the expression and purification of the NC fusion proteins from yeast. To determine its antigenicity, the fusion protein was challenged with serum samples from SARS patients and normal controls. The NC fusion protein demonstrated high antigenicity with high specificity, and therefore, it should have great potential in designing clinical diagnostic tools and provide useful information for vaccine development.展开更多
文摘Dinitrobenzene(1,4-DNB) is one of the preferential toxicants stipulated by OECD and EPA of USA. With Oryzias latipes, which were chosen as the acceptors according to the international standard, the acute, subacute and accumulative toxicity experiments were conducted. The threshold mass concentration of O.latipes was 1.360 mg/L. The subacute experiment showed that O.latipes were hardly affected when the mass concentration of 1,4-DNB was 0.0292 mg/L, but were significantly affected when the mass concentration of 1,4-DNB was 0.0420, 0.0608 or 0.0875 mg/L in contrast to the control sample(P<0.05). The accumulative rate(K) was 1.21. This shows that there are significant accumulative actions of 1,4-DNB in fish. When the mass concentration of 1,4-DNB is in the range of 0.032_0.320 mg/L, the higher the 1,4-DNB concentration, the more strongly the Na +/K +-ATPase is inhibited. The calculated IC_ 50 values in gill, kidney and liver of O. latipes are 0.128, 0.132 and 0.428 mg/L, respectively.
文摘In the whole power supply system, the transmission line is a very key link, and the transmission line determines whether the electricity can be transported smoothly, whether the power grid can operate safely and stably. Power system transmission line project has the characteristics of long construction time, many participants, high quality risk and large investment, and has certain particularity compared with ordinary construction projects. This paper uses the investigation method, literature method and analysis method to focus on the transmission line management and quality control method for reference.
文摘In the development process of state-owned enterprises, it is necessary to constantly improve the management system, pay attention to the improvement of human resource management system, so as to mobilize the enthusiasm of human resources, enhance the enthusiasm of personnel, and introduces more foreign high-quality foreign talents. With the continuous development of the market economy, the reform of state-owned enterprises should meet the corresponding requirements, actively change the management concept, encourage personnel ideological innovation, especially in the aspect of human resource management, strengthen the performance appraisal and salary management, optimize the personnel structure, and activate peoples ideological vitality.
文摘In order to develop clinical diagnostic tools for rapid detection of SARS-CoV (severe acute respiratory syndrome-associated coronavirus) and to identify candidate proteins for vaccine development, the C-terminal portion of the nucleocapsid (NC) gene was amplified using RT-PCR from the SARS-CoV genome, cloned into a yeast expression vector (pEGH), and expressed as a glutathione S-transferase (GST) and Hisx6 double-tagged fusion protein under the control of an inducible promoter. Western analysis on the purified protein confirmed the expression and purification of the NC fusion proteins from yeast. To determine its antigenicity, the fusion protein was challenged with serum samples from SARS patients and normal controls. The NC fusion protein demonstrated high antigenicity with high specificity, and therefore, it should have great potential in designing clinical diagnostic tools and provide useful information for vaccine development.
