Among plant hormones,cytokinins have been extensively studied for over a century due to their central roles in regulating plant development,physiology,and environmental responses.In this study,we chemically synthesize...Among plant hormones,cytokinins have been extensively studied for over a century due to their central roles in regulating plant development,physiology,and environmental responses.In this study,we chemically synthesized several Cytokinin-Active ingreDients(CADs)and evaluated their effects on the in vitro regeneration of Sansevieria trifasciata and seedling growth of Nicotiana tabacum,comparing their activities with established cytokinins,kinetin and zeatin.Our results show that CADs promote cell division and shoot proliferation in S.trifasciata,with the highest shoot numbers per explant observed at CAD-A(0.9 mg L^(-1)),cycle-iP(0.1 mg L^(-1)),and zeatin(0.3 mg L^(-1)),all significantly different from kinetin(0.3 mg L^(-1))—the previously suggested cytokinin for in vitro shoot proliferation in this plant.CAD-A and CAD-B at 0.9 mg L^(-1)increased leaf number by approximately 4.5,significantly outperforming kinetin at 0.3 mg L^(-1).In tobacco seedlings,CAD-B at 0.3 mg L^(-1)significantly enhanced root number(7.3)comparable to kinetin(6.7)at the same concentration.Overall seedling height increased more than two-fold under both CAD-B and kinetin treatments compared to control plants.The bioactivity of CADs under in vitro conditions closely parallels that of kinetin,supporting their potential as effective cytokinin mimetics.Collectively,these results indicate that synthesized CADs can serve as potent alternatives to natural cytokinins for plant tissue culture and developmental studies,with promising applications in propagation and regeneration protocols.展开更多
文摘Among plant hormones,cytokinins have been extensively studied for over a century due to their central roles in regulating plant development,physiology,and environmental responses.In this study,we chemically synthesized several Cytokinin-Active ingreDients(CADs)and evaluated their effects on the in vitro regeneration of Sansevieria trifasciata and seedling growth of Nicotiana tabacum,comparing their activities with established cytokinins,kinetin and zeatin.Our results show that CADs promote cell division and shoot proliferation in S.trifasciata,with the highest shoot numbers per explant observed at CAD-A(0.9 mg L^(-1)),cycle-iP(0.1 mg L^(-1)),and zeatin(0.3 mg L^(-1)),all significantly different from kinetin(0.3 mg L^(-1))—the previously suggested cytokinin for in vitro shoot proliferation in this plant.CAD-A and CAD-B at 0.9 mg L^(-1)increased leaf number by approximately 4.5,significantly outperforming kinetin at 0.3 mg L^(-1).In tobacco seedlings,CAD-B at 0.3 mg L^(-1)significantly enhanced root number(7.3)comparable to kinetin(6.7)at the same concentration.Overall seedling height increased more than two-fold under both CAD-B and kinetin treatments compared to control plants.The bioactivity of CADs under in vitro conditions closely parallels that of kinetin,supporting their potential as effective cytokinin mimetics.Collectively,these results indicate that synthesized CADs can serve as potent alternatives to natural cytokinins for plant tissue culture and developmental studies,with promising applications in propagation and regeneration protocols.
