Apples,as respiratory climacteric fruit,undergo postharvest ripening and senescence,impacting commodity value.Cuticular wax protects against environmental stresses.Here,gas chromatography-mass spectrometry(GC-MS)analy...Apples,as respiratory climacteric fruit,undergo postharvest ripening and senescence,impacting commodity value.Cuticular wax protects against environmental stresses.Here,gas chromatography-mass spectrometry(GC-MS)analysis revealed a decline in fatty alcohol levels in apple wax during storage,correlating with fruit quality deterioration.Notably,octacosanol content signifcantly decreased with storage,suggesting that it is a primary cause of wax and fruit quality decline.Octacosanol treatment improved fruit quality and delayed softening by enhancing wax synthesis and antioxidant levels and suppressing cell wall-degrading enzymes.Transcriptome sequencing and real-time quantitative polymerase chain reaction(RT-qPCR)assays indicated increased expression of wax,peroxidase,sucrose,and starch genes and decreased expression of cell wall degradation genes,explaining octacosanol’s benefts.This study provides a theoretical basis for octacosanol application in fruit preservation.展开更多
基金supported by the grants from the National Key Research and Development Program of China(Nos.2023YFD2301000 and 2022YFD2100102)the National Natural Science Foundation of China(Nos.32122080 and 32302616)+2 种基金the Key Research and Development Program of Shandong Province,China(No.2023CXGC010709)the Natural Science Foundation of Shandong Province,China(No.ZR2023QC032)the National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops,China(No.Horti-KF-2023-11).
文摘Apples,as respiratory climacteric fruit,undergo postharvest ripening and senescence,impacting commodity value.Cuticular wax protects against environmental stresses.Here,gas chromatography-mass spectrometry(GC-MS)analysis revealed a decline in fatty alcohol levels in apple wax during storage,correlating with fruit quality deterioration.Notably,octacosanol content signifcantly decreased with storage,suggesting that it is a primary cause of wax and fruit quality decline.Octacosanol treatment improved fruit quality and delayed softening by enhancing wax synthesis and antioxidant levels and suppressing cell wall-degrading enzymes.Transcriptome sequencing and real-time quantitative polymerase chain reaction(RT-qPCR)assays indicated increased expression of wax,peroxidase,sucrose,and starch genes and decreased expression of cell wall degradation genes,explaining octacosanol’s benefts.This study provides a theoretical basis for octacosanol application in fruit preservation.
