目的分析不明原因复发性流产(unexplained recurrent spontaneous abortion,URSA)患者蜕膜组织中基质金属蛋白酶(matrix metalloproteinases,MMPs)和基质金属蛋白酶组织抑制物(tissue inhibitor of metalloproteinases,TIMPs)的表达及...目的分析不明原因复发性流产(unexplained recurrent spontaneous abortion,URSA)患者蜕膜组织中基质金属蛋白酶(matrix metalloproteinases,MMPs)和基质金属蛋白酶组织抑制物(tissue inhibitor of metalloproteinases,TIMPs)的表达及其启动区甲基化水平。方法选取2017年1月至2017年12月在我院妇产科行清宫术的URSA患者31例(URSA组)及同期正常早孕放弃妊娠行人工流产女性30例(NC组)的蜕膜组织,应用实时定量PCR技术分别检测MMP1、MMP2、MMP3、MMP7、MMP9、TIMP1、TIMP2、TIMP3以及TIMP4的表达情况。焦磷酸测序技术检测上述差异表达基因启动区的甲基化水平。结果两组患者在年龄、停经天数等无统计学差异(P>0.05)。URSA组蜕膜中MMP2水平(1.08±0.68 vs 2.15±0.97)、MMP9水平(0.83±0.79 vs 2.01±1.42)的表达水平较NC组下调。URSA组较NC组TIMP1(3.45±1.33 vs 2.16±1.57)和TIMP2(2.95±1.14 vs1.72±1.29)TIMP3(2.8±1.1 vs1.62±1.16)的表达水平上调,差异均具有统计学意义(P<0.05)。MMP1、MMP3、MMP7和TIMP4在两组间表达差异无统计学意义(P>0.05)。对转录水平存在差异的MMP2、MMP9、TIMP1、TIMP2、TIMP3启动区甲基化水平进行焦磷酸测序,结果显示,URSA组MMP2启动区甲基化水平(0.26±0.11)高于NC组(0.18±0.07),TIMP3启动区甲基化水平(0.46±0.10)低于NC组(0.63±0.11),差异均有统计学意义(P<0.05)。结论URSA妇女的蜕膜组织中MMP2、MMP9的表达水平下调,TIMP1、TIMP2、TIMP3的表达水平上调,出现MMPs/TIMPs失衡,考虑细胞外基质水解不足导致了绒毛组织的的侵袭力下降为URSA可能的发病机制,而其中MMP2、TIMP3的基因表达与其启动区甲基化水平的改变有关。展开更多
To build a rapid and accurate method for greenhouse vegetable landinformation extraction using an index model derived from TM digital data of Qingzhou City, ShandongProvince, based on a systematic analysis of the spec...To build a rapid and accurate method for greenhouse vegetable landinformation extraction using an index model derived from TM digital data of Qingzhou City, ShandongProvince, based on a systematic analysis of the spectral characteristics of different land use typesin the study area, a subset of the image was first made to eliminate the mountainous region notassociated with vegetable distribution, and then water body pixels were masked. With this the V_Iindex model for greenhouse vegetable land extraction was developed. The index model indicatedgreenhouse vegetable land for Qingzhou in April 2002 was concentrated in the southeast and aroundrural residential areas. Field data used for an accuracy evaluation showed that greenhouse hectaresdetermined with remote sensing were 95.9% accurate, and accuracy for the spatial distribution ofgreenhouse vegetable land cross checked with a random sample was 96.3%. Therefore, this approachprovided an effective method for greenhouse vegetable land information extraction and has potentialsignificance for management of greenhouse vegetable production in the study area, as well as NorthChina.展开更多
A new technique was introduced for sand stabilization and re-vegetation by use of lignin sand stabilizing material(LSSM). LSSM is a reconstructed organic compound with lignin as the most dominant component from the ex...A new technique was introduced for sand stabilization and re-vegetation by use of lignin sand stabilizing material(LSSM). LSSM is a reconstructed organic compound with lignin as the most dominant component from the extracts of black-liquor issued by straw pulp paper mills. Unlike the polyvinyl acetate or foamed asphalt commonly used for dune stabilization, the new material is plant-friendly and can be used with virescence actions simultaneously. The field experimental study was conducted since 2001 in China's Northwest Ningxia Hui Autonomous Region and has been proved that LSSM is effective in stabilizing the fugitive dunes, making the arenaceous plants survive and the bare dune vegetative. The advisable solution concentration is 2% and the optimal field spraying quantity is 2 5 L/m^2 The soil nutrients of the stabilized and greened dune, such as organic matter, available phosphorous and total nitrogen are all increased compared with the control treatment, which is certainly helpful to the growth of arenaceous plants. The technique is worthwhile to be popularized because it is provided not only a new method for desertification control but also an outlet for cleaning contaminants issued from the straw paper mills.展开更多
Glaucophane in Shuangjiang area, West Yunnan Province, supplies a chance for studying south segment of Lancangjiang tectonic zone. But people are at odds as to whether two stage glaucophane exists or not, glaucophane...Glaucophane in Shuangjiang area, West Yunnan Province, supplies a chance for studying south segment of Lancangjiang tectonic zone. But people are at odds as to whether two stage glaucophane exists or not, glaucophane is the result of dynamic metamorphism later, or indicates a high P/T metamorphic belt when Paleozoic Tethys Sea closed. Authors discover in a recent research that there is only one stage glaucophane in Shuangjiang area, and three blueschist belts are distributed near N S tending, and glaucophane in Shuangjiang area is related to the eastward subduction of Changning Menglian basin.展开更多
文摘目的分析不明原因复发性流产(unexplained recurrent spontaneous abortion,URSA)患者蜕膜组织中基质金属蛋白酶(matrix metalloproteinases,MMPs)和基质金属蛋白酶组织抑制物(tissue inhibitor of metalloproteinases,TIMPs)的表达及其启动区甲基化水平。方法选取2017年1月至2017年12月在我院妇产科行清宫术的URSA患者31例(URSA组)及同期正常早孕放弃妊娠行人工流产女性30例(NC组)的蜕膜组织,应用实时定量PCR技术分别检测MMP1、MMP2、MMP3、MMP7、MMP9、TIMP1、TIMP2、TIMP3以及TIMP4的表达情况。焦磷酸测序技术检测上述差异表达基因启动区的甲基化水平。结果两组患者在年龄、停经天数等无统计学差异(P>0.05)。URSA组蜕膜中MMP2水平(1.08±0.68 vs 2.15±0.97)、MMP9水平(0.83±0.79 vs 2.01±1.42)的表达水平较NC组下调。URSA组较NC组TIMP1(3.45±1.33 vs 2.16±1.57)和TIMP2(2.95±1.14 vs1.72±1.29)TIMP3(2.8±1.1 vs1.62±1.16)的表达水平上调,差异均具有统计学意义(P<0.05)。MMP1、MMP3、MMP7和TIMP4在两组间表达差异无统计学意义(P>0.05)。对转录水平存在差异的MMP2、MMP9、TIMP1、TIMP2、TIMP3启动区甲基化水平进行焦磷酸测序,结果显示,URSA组MMP2启动区甲基化水平(0.26±0.11)高于NC组(0.18±0.07),TIMP3启动区甲基化水平(0.46±0.10)低于NC组(0.63±0.11),差异均有统计学意义(P<0.05)。结论URSA妇女的蜕膜组织中MMP2、MMP9的表达水平下调,TIMP1、TIMP2、TIMP3的表达水平上调,出现MMPs/TIMPs失衡,考虑细胞外基质水解不足导致了绒毛组织的的侵袭力下降为URSA可能的发病机制,而其中MMP2、TIMP3的基因表达与其启动区甲基化水平的改变有关。
基金Project supported by the Chinese Ministry of Education (No. [2002] 247).
文摘To build a rapid and accurate method for greenhouse vegetable landinformation extraction using an index model derived from TM digital data of Qingzhou City, ShandongProvince, based on a systematic analysis of the spectral characteristics of different land use typesin the study area, a subset of the image was first made to eliminate the mountainous region notassociated with vegetable distribution, and then water body pixels were masked. With this the V_Iindex model for greenhouse vegetable land extraction was developed. The index model indicatedgreenhouse vegetable land for Qingzhou in April 2002 was concentrated in the southeast and aroundrural residential areas. Field data used for an accuracy evaluation showed that greenhouse hectaresdetermined with remote sensing were 95.9% accurate, and accuracy for the spatial distribution ofgreenhouse vegetable land cross checked with a random sample was 96.3%. Therefore, this approachprovided an effective method for greenhouse vegetable land information extraction and has potentialsignificance for management of greenhouse vegetable production in the study area, as well as NorthChina.
文摘A new technique was introduced for sand stabilization and re-vegetation by use of lignin sand stabilizing material(LSSM). LSSM is a reconstructed organic compound with lignin as the most dominant component from the extracts of black-liquor issued by straw pulp paper mills. Unlike the polyvinyl acetate or foamed asphalt commonly used for dune stabilization, the new material is plant-friendly and can be used with virescence actions simultaneously. The field experimental study was conducted since 2001 in China's Northwest Ningxia Hui Autonomous Region and has been proved that LSSM is effective in stabilizing the fugitive dunes, making the arenaceous plants survive and the bare dune vegetative. The advisable solution concentration is 2% and the optimal field spraying quantity is 2 5 L/m^2 The soil nutrients of the stabilized and greened dune, such as organic matter, available phosphorous and total nitrogen are all increased compared with the control treatment, which is certainly helpful to the growth of arenaceous plants. The technique is worthwhile to be popularized because it is provided not only a new method for desertification control but also an outlet for cleaning contaminants issued from the straw paper mills.
文摘Glaucophane in Shuangjiang area, West Yunnan Province, supplies a chance for studying south segment of Lancangjiang tectonic zone. But people are at odds as to whether two stage glaucophane exists or not, glaucophane is the result of dynamic metamorphism later, or indicates a high P/T metamorphic belt when Paleozoic Tethys Sea closed. Authors discover in a recent research that there is only one stage glaucophane in Shuangjiang area, and three blueschist belts are distributed near N S tending, and glaucophane in Shuangjiang area is related to the eastward subduction of Changning Menglian basin.
