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柑橘木虱黏液样蛋白DcMucin-like抗体制备及应用
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作者 蒋丽琴 苏巧灵 +2 位作者 李猷 魏太云 宾羽 《中国农业科学》 北大核心 2025年第2期281-290,共10页
【目的】黏液样蛋白(mucin-like protein)参与半翅目昆虫取食过程中唾液鞘的形成。本研究旨在制备柑橘木虱(Diaphorina citri)黏液样蛋白DcMucin-like特异性抗体并对木虱取食孔进行免疫荧光标记,为DcMucin-like生物学功能研究提供依据... 【目的】黏液样蛋白(mucin-like protein)参与半翅目昆虫取食过程中唾液鞘的形成。本研究旨在制备柑橘木虱(Diaphorina citri)黏液样蛋白DcMucin-like特异性抗体并对木虱取食孔进行免疫荧光标记,为DcMucin-like生物学功能研究提供依据。【方法】通过解剖柑橘木虱唾液腺、中肠、卵巢和精巢组织,根据木虱DcMcuin-like序列设计特异性引物,利用实时荧光定量PCR检测分析各组织中DcMcuin-like在转录水平的差异;扩增无信号肽的DcMucin-like序列并插入pET-28a中构建重组质粒,测序验证后转化Rosetta表达菌株,以终浓度0.5 mmol·L-1 IPTG在37℃下振荡培养8 h诱导重组蛋白表达,SDS-PAGE凝胶电泳检测重组蛋白表达情况;扩大培养后裂解菌体,裂解液上清经Ni-NTA纯化作为抗原免疫家兔5次,纯化血清IgG获得DcMucin-like多克隆抗体,Western blot检测抗体特异性;利用实时荧光定量PCR和Western blot比较健康木虱和感染黄龙病菌(Candidatus Liberibacter asiaticus,CLas)木虱中DcMucin-like在转录水平和蛋白水平的差异;利用交联荧光素(FITC)的DcMucin-like多克隆抗体标记木虱取食后的柑橘叶片,在共聚焦显微镜下观察柑橘木虱取食孔和唾液鞘。【结果】实时荧光定量PCR检测显示DcMcuin-like在木虱唾液腺中大量表达,且表达量显著高于中肠、卵巢和精巢组织。携带有pET28a-DcMucin的Rosetta表达菌株经IPTG诱导后在菌体裂解液的上清中获得大量表达的重组蛋白。重组蛋白免疫家兔后取抗血清纯化IgG,Western blot结果表明获得特异性好的DcMucin-like多克隆抗体。实时荧光定量PCR和Western blot分析结果显示DcMucin-like在木虱响应黄龙病菌侵染时上调表达。DcMucin-like(FITC)荧光抗体标记木虱取食后的柑橘叶片组织切片,共聚焦显微镜下观察到木虱的取食孔,并在取食孔周围检测到特异的FITC荧光信号,表明DcMucin-like随木虱取食释放到植株组织中参与唾液鞘形成。【结论】DcMucin-like在柑橘木虱唾液腺中高表达并响应黄龙病菌侵染上调表达;成功制备了特异性好的柑橘木虱DcMucin-like多克隆抗体;验证了DcMucin-like随木虱取食分泌到柑橘植株组织中。研究结果可为深入探究DcMucin-like在黄龙病菌-木虱-柑橘三者互作中的作用打下基础。 展开更多
关键词 柑橘木虱 唾液鞘蛋白 DcMucin-like 抗体制备 免疫荧光
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Prediction of malignancy and adverse outcome of solid pseudopapillary tumor of the pancreas 被引量:19
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作者 li you Feng Yang De--liang Fu 《World Journal of Gastrointestinal Oncology》 SCIE CAS 2018年第7期184-193,共10页
Since solid pseudopapillary tumor of the pancreas(SP-TP) was officially classified by the World Health Org-anization in 1996,SPTP has recently received special attention in the literature.Studies have shown that SPTP ... Since solid pseudopapillary tumor of the pancreas(SP-TP) was officially classified by the World Health Org-anization in 1996,SPTP has recently received special attention in the literature.Studies have shown that SPTP is a heterogeneous tumor,with a small percentage of patients harboring aggressive behaviors.However,cri-teria for malignancy grade in SPTP have not been well established.The prognosis of SPTP is generally good,with cases having a chance for long-term survival even with recurrence and/or metastasis after surgical resection.The current American Joint Committee on Cancer/Union for International Cancer Control tumor,node,metastasis staging system is not specific to SPTP.The lack of a pre-dictive staging classification that accurately describes the heterogeneity of this disease hinders meaningful research into optimal individualized therapy.Here we summarize and discuss the associated factors proposed for appraisal of the malignant potential and adverse outcome of SPTP. 展开更多
关键词 PANCREAS RECURRENCE SOLID pseudopapillary TUMOR MALIGNANCY METASTASIS
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基于遥感指数的我国西部矿区生态质量监测——以彬长矿区为例 被引量:5
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作者 姚顽强 李悠 +4 位作者 郑俊良 蔺小虎 相里海龙 惠伯辰 李文欣 《金属矿山》 北大核心 2025年第1期259-266,共8页
我国西部矿区是煤炭资源开采的主要地区,也是最具代表性的生态脆弱区,探讨西部矿区自然生态环境的演变趋势及影响机制,对于矿区自然环境保护与有序恢复有着重要意义。以彬长矿区为例,通过遥感和GIS技术,利用Sentinel-2和Landsat 8遥感... 我国西部矿区是煤炭资源开采的主要地区,也是最具代表性的生态脆弱区,探讨西部矿区自然生态环境的演变趋势及影响机制,对于矿区自然环境保护与有序恢复有着重要意义。以彬长矿区为例,通过遥感和GIS技术,利用Sentinel-2和Landsat 8遥感影像数据,基于遥感生态指数(Remote Sensing Ecological Index,RSEI)模型,在深入剖析该矿区土地利用/覆被变化(Land Use/Cover Change,LUCC)和生态环境时空演变的基础上,分析了该矿区LUCC的生态质量。结果表明:①2017—2022年矿区LUCC结构上出现明显的转移特征,形成以林地、耕地和建设用地为主的新LUCC格局,并伴随着林地大范围转出为耕地和建设用地的情况,转移量约191.04 km^(2)。②5 a中,矿区生态环境质量呈下降趋势,RSEI由0.54降至0.47,研究发现矿区生态环境质量主要受绿度和干度指标因子影响。③2017—2022年矿区生态环境质量改善的面积占总面积的9%,生态环境质量恶化的面积占总面积的22%,分析结果可为西部矿区生态环境保护和修复提供参考。 展开更多
关键词 生态修复 遥感 GIS 遥感生态指数 土地利用/覆被变化 生态质量
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Self-supporting nanoporous Ni/metallic glass composites with hierarchically porous structure for efficient hydrogen evolution reaction 被引量:4
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作者 Jing Wang li you +8 位作者 Zhibin li Xiongjun liu Rui li Qing Du Xianzhen Wang Hui Wang Yuan Wu Suihe Jiang Zhaoping Lu 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2021年第14期145-150,共6页
Searching for free-standing and cost-efficient hydrogen evolution reaction(HER)electrocatalysts with high efficiency and excellent durability remains a great challenge for the hydrogen-based energy industry.Here,we re... Searching for free-standing and cost-efficient hydrogen evolution reaction(HER)electrocatalysts with high efficiency and excellent durability remains a great challenge for the hydrogen-based energy industry.Here,we report fabrication of a unique hierarchically porous structure,i.e.,nanoporous Ni(NPN)/metallic glass(MG)composite,through surface dealloying of the specially designed Ni_(40)Zr_(40)Ti_(20)MG wire.This porous composite is composed of micrometer slits staggered with nanometer pores,which not only enlarges effective surface areas for the catalytic reaction,but also facilitates the release of H2 gas.As a result,the NPN/MG hybrid electrode exhibited the prominent HER performance with a low overpotential of 78 m V at 10 m A cm^(-2)and Tafel slope of 42.4 m V dec^(-1),along with outstanding stability in alkaline solutions.Outstanding catalytic properties,combining with their free-standing capability and cost efficiency,make the current composite electrode viable for HER applications. 展开更多
关键词 Nanoporous Ni DEALLOYING Metallic glasses precursor Hierarchically porous structure Hydrogen evolution reaction
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An magnetic resonance-based plasma metabonomic investigation on abnormal Savda in different complicated diseases 被引量:5
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作者 Batur Mamtimin Mawlanjan Hizbulla +3 位作者 Nazuk Kurbantay li you Xinghai Yan Halmurat Upur 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2014年第2期166-172,共7页
OBJECTIVE: To provide existence of abnormal potential evidence for the Savda, we assessed host metabonomic responses and dynamic changes oc- curring in various diseases using 1H nuclear mag- netic resonance (NMR)-b... OBJECTIVE: To provide existence of abnormal potential evidence for the Savda, we assessed host metabonomic responses and dynamic changes oc- curring in various diseases using 1H nuclear mag- netic resonance (NMR)-based metabonomics. METHODS: Plasma samples taken from patients with complicated diseases with abnormal Savda (n= 140, including 35 cases each of diabetes, asthma, breast cancer, and cervical carcinoma) and from healthy controls (n=35) were analyzed by ~H NMR (600 MHz), and the spectral profiles were analyzed by multivariate analysis using orthogonal projec- tion to latent structure with discriminant analysis. RESULTS: Supervised modeling of the data provid- ed very good discrimination between patients and healthy controls. Compared with ,the healthy controls, the patient groups with different disease con- ditions displayed similar metabolic changes, characterized by lower creatine, creatinine, lactate, and amino acid levels (including isoleucine, leucine, va- line, alanine, and 1-methylhistidine) and higher lip- id levels (very low-density lipoproteins and unsatu- rated lipids). Additionally, cancer patients (breast and cervical) showed decreased myo-inositol, a-glucose, and 13-glucose, and increased pyruvate and carnitine in plasma. CONCLUSION: The data indicate that decreased ox- idative defense, liver function abnormalities, amino acid deficiencies, and energy metabolism disorders are common characteristics of complicated diseas- es, which may be related to the formation of abnor- mal Savda. 展开更多
关键词 METABONOMICS Magnetic resonancespectroscopy Abnormal Savda
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LoS MIMO Transmission for LEO Satellite Communication Systems 被引量:3
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作者 lingxuan li Tingting Chen +3 位作者 Wenjin Wang Xiaohang Song li you Xiqi Gao 《China Communications》 SCIE CSCD 2022年第10期180-193,共14页
To provide global service with low latency, the broadband low earth orbits (LEO) satellite constellation based communication systems have become one of the focuses in academic and industry. To allow for wideband acces... To provide global service with low latency, the broadband low earth orbits (LEO) satellite constellation based communication systems have become one of the focuses in academic and industry. To allow for wideband access for user links, the feeder link of LEO satellite is correspondingly required to support high throughput data communications. To this end, we propose to apply line-of-sight (LoS) multiple-input multiple-output (MIMO) transmission for the feeder link to achieve spatial multiplexing by optimizing the antenna arrangement. Unlike the LoS MIMO applications for static scenarios, the movement of LEO satellites make it impractical to adjust the optimal antenna separation for all possible satellite positions. To address this issue, we propose to design the antenna placement to maximize the ergodic channel capacity during the visible region of the ground station. We first derive the closed-form probability distribution of the satellite trajectory in visible region. Based on which the ergodic channel capacity can be then calculated numerically. The antenna placement can be further optimized to maximize the ergodic channel capacity. Numerical results verify the derived probability distribution of the satellite trajectory, and show that the proposed LoS MIMO scheme can significantly increase the ergodic channel capacity compared with the existing SISO one. 展开更多
关键词 LoS MIMO LEO satellite ergodic channel capacity Beyond 5G
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Air combat target maneuver trajectory prediction based on robust regularized Volterra series and adaptive ensemble online transfer learning 被引量:2
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作者 Xi Zhi-fei Kou Ying-xin +4 位作者 li Zhan-wu Lv Yue Xu An li you li Shuang-qing 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2023年第2期187-206,共20页
Target maneuver trajectory prediction is an important prerequisite for air combat situation awareness and maneuver decision-making.However,how to use a large amount of trajectory data generated by air combat confronta... Target maneuver trajectory prediction is an important prerequisite for air combat situation awareness and maneuver decision-making.However,how to use a large amount of trajectory data generated by air combat confrontation training to achieve real-time and accurate prediction of target maneuver trajectory is an urgent problem to be solved.To solve this problem,in this paper,a hybrid algorithm based on transfer learning,online learning,ensemble learning,regularization technology,target maneuvering segmentation point recognition algorithm,and Volterra series,abbreviated as AERTrOS-Volterra is proposed.Firstly,the model makes full use of a large number of trajectory sample data generated by air combat confrontation training,and constructs a Tr-Volterra algorithm framework suitable for air combat target maneuver trajectory prediction,which realizes the extraction of effective information from the historical trajectory data.Secondly,in order to improve the real-time online prediction accuracy and robustness of the prediction model in complex electromagnetic environments,on the basis of the TrVolterra algorithm framework,a robust regularized online Sequential Volterra prediction model is proposed by integrating online learning method,regularization technology and inverse weighting calculation method based on the priori error.Finally,inspired by the preferable performance of models ensemble,ensemble learning scheme is also incorporated into our proposed algorithm,which adaptively updates the ensemble prediction model according to the performance of the model on real-time samples and the recognition results of target maneuvering segmentation points,including the adaptation of model weights;adaptation of parameters;and dynamic inclusion and removal of models.Compared with many existing time series prediction methods,the newly proposed target maneuver trajectory prediction algorithm can fully mine the prior knowledge contained in the historical data to assist the current prediction.The rationality and effectiveness of the proposed algorithm are verified by simulation on three sets of chaotic time series data sets and a set of real target maneuver trajectory data sets. 展开更多
关键词 Maneuver trajectory prediction Volterra series Transfer learning Online learning Ensemble learning Robust regularization
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Partial pancreatic tail preserving subtotal pancreatectomy forpancreatic cancer: Improving glycemic control and quality of lifewithout compromising oncological outcomes 被引量:3
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作者 li you lie Yao +3 位作者 Yi-Shen Mao Cai-Feng Zou Chen Jin De-liang Fu 《World Journal of Gastrointestinal Surgery》 SCIE 2020年第12期491-506,共16页
BACKGROUND Total pancreatectomy (TP) is usually considered a therapeutic option forpancreatic cancer in which Whipple surgery and distal pancreatectomy areundesirable, but brittle diabetes and poor quality of life (Qo... BACKGROUND Total pancreatectomy (TP) is usually considered a therapeutic option forpancreatic cancer in which Whipple surgery and distal pancreatectomy areundesirable, but brittle diabetes and poor quality of life (QoL) remain majorconcerns. A subset of patients who underwent TP even died due to severehypoglycemia. For pancreatic cancer involving the pancreatic head and proximalbody but without invasion to the pancreatic tail, we performed partial pancreatictail preserving subtotal pancreatectomy (PPTP-SP) in selected patients, in order toimprove postoperative glycemic control and QoL without compromisingoncological outcomes.AIM To evaluate the efficacy of PPTP-SP for patients with pancreatic cancer.METHODS We retrospectively reviewed 56 patients with pancreatic ductal adenocarcinomawho underwent PPTP-SP (n = 18) or TP (n = 38) at our institution from May 2014to January 2019. Clinical outcomes were compared between the two groups, withan emphasis on oncological outcomes, postoperative glycemic control, and QoL.QoL was evaluated using the European Organization for Research and Treatmentof Cancer Quality of Life Questionnaire (EORTC QLQ-C30 and EORTC PAN26).All patients were followed until May 2019 or until death.RESULTS A total of 56 consecutive patients were enrolled in this study. Perioperativeoutcomes, recurrence-free survival, and overall survival were comparablebetween the two groups. No patients in the PPTP-SP group developed cancerrecurrence in the pancreatic tail stump or splenic hilum, or a clinical pancreaticfistula. Patients who underwent PPTP-SP had significantly better glycemiccontrol, based on their higher rate of insulin-independence (P = 0.014), lowerhemoglobin A1c (HbA1c) level (P = 0.046), lower daily insulin dosage (P < 0.001),and less frequent hypoglycemic episodes (P < 0.001). Global health was similar inthe two groups, but patients who underwent PPTP-SP had better functional status(P = 0.036), milder symptoms (P = 0.013), less severe diet restriction (P = 0.011),and higher confidence regarding future life (P = 0.035).CONCLUSION For pancreatic cancer involving the pancreatic head and proximal body, PPTP-SPachieves perioperative and oncological outcomes comparable to TP in selectedpatients while significantly improving long-term glycemic control and QoL. 展开更多
关键词 Partial pancreatic tail preserving subtotal pancreatectomy Total pancreatectomy Pancreatic cancer Treatment outcome Diabetes mellitus Quality of life
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危重症患儿肠内营养喂养不耐受危险因素分析及预测模型的构建
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作者 周霞 高红梅 +4 位作者 黄琳 韩辉武 胡红玲 李莜 虞仁和 《中国当代儿科杂志》 北大核心 2025年第3期321-327,共7页
目的探讨危重患儿肠内营养(enteral nutrition,EN)发生喂养不耐受(feeding intolerance,FI)的危险因素,并构建列线图模型。方法回顾性收集2015年1月—2020年10月中南大学湘雅医院儿童重症监护室收治的危重患儿资料,随机分为训练集组(346... 目的探讨危重患儿肠内营养(enteral nutrition,EN)发生喂养不耐受(feeding intolerance,FI)的危险因素,并构建列线图模型。方法回顾性收集2015年1月—2020年10月中南大学湘雅医院儿童重症监护室收治的危重患儿资料,随机分为训练集组(346例)和验证集组(147例)。训练集组进一步分为耐受组(216例)和不耐受组(130例)。采用多因素logistic回归分析筛选危重患儿EN发生FI的危险因素,利用R语言构建列线图,对验证集组进行验证。分别采用受试者操作特征曲线、校准曲线和决策曲线分析评价模型的区分度、校准度和临床净收益。结果卧床时间、休克、胃肠减压、使用非甾体类抗炎药、联合使用肠外营养是危重患儿EN发生FI的独立危险因素(P<0.05)。基于这些因素构建EN危重患儿发生FI的列线图预测模型,受试者操作特征曲线分析结果显示,训练集组和验证集组曲线下面积分别为0.934(95%CI:0.906~0.963)和0.852(95%CI:0.787~0.917),表明模型的区分度良好。Hosmer-Lemeshow拟合优度检验表明该模型拟合度良好(χ^(2)=12.559,P=0.128)。校准曲线分析和决策曲线分析提示该模型的预测效能和临床应用价值较高。结论卧床时间、休克、胃肠减压、使用非甾体类抗炎药物和联合使用肠外营养是危重患儿EN发生FI的独立危险因素;根据这些因素构建的列线图模型有较高的预测效能和临床应用价值。 展开更多
关键词 喂养不耐受 肠内营养 危险因素 列线图 预测模型 儿童
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“志意失畅”致焦虑:基于形神一体观的情志致病新假说的初步构建
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作者 高明周 胡明会 +7 位作者 董洪玮 李由 赵月 王新宇 李自发 耿希文 魏盛 张浩 《中国实验动物学报》 北大核心 2025年第9期1320-1328,共9页
焦虑作为一种主要的情绪障碍,表现为个体对未来威胁的预期,发病率约为7.3%,终生患病率位居首位,且与抑郁存在重叠机制,为多种精神疾病(中医情志病证)的典型症状,共病率及致残率高,严重危害人民健康。动物模型作为研究焦虑的重要载体,对... 焦虑作为一种主要的情绪障碍,表现为个体对未来威胁的预期,发病率约为7.3%,终生患病率位居首位,且与抑郁存在重叠机制,为多种精神疾病(中医情志病证)的典型症状,共病率及致残率高,严重危害人民健康。动物模型作为研究焦虑的重要载体,对破解焦虑发病机制及研发药物具有重要意义。但传统上的应激致焦虑公认范式却较为局限,本文基于“传统理论+临床数据+动物实验数据”初步提出一种“志意失畅”致焦虑新假说,定义为“个体经过多次尝试后自身需求无法满足、需求受限或被剥夺,是一种因愿望受阻又无能为力时所诱发的焦虑状态”,本假说把现代医学焦虑临床患者绝望放弃、快感缺乏和社交退缩的典型表现,与“饥而不能食,食而不能得,得而不能饱”的传统中医情志病证理论建立联系提出“志意失畅”致焦虑的中医病机观点,并在一种仿真饲料设计及造模的动物实验中得到数据支撑,据此建立的造模范式易于操作、重复性好、成本低廉,可用于建立大小鼠焦虑模型,为抗焦虑药物的研发及药效学评价提供理论和模型依据。 展开更多
关键词 情志病 焦虑 新假说 机制
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场地土壤污染物三维分布刻画:非平稳解耦、数据纠偏与模型选择的三元关系视角
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作者 陶欢 李尤 +1 位作者 侯艺璇 廖晓勇 《地理科学进展》 北大核心 2025年第9期1765-1778,共14页
土壤污染物的三维精细刻画是场地环境管理中实现精准治理与科学决策的关键环节。污染物浓度的空间非平稳性与钻井数据的稀疏有偏性是限制现有三维刻画模型精度和刻画结果可靠性的核心挑战。论文从“非平稳解耦、数据纠偏与模型选择”的... 土壤污染物的三维精细刻画是场地环境管理中实现精准治理与科学决策的关键环节。污染物浓度的空间非平稳性与钻井数据的稀疏有偏性是限制现有三维刻画模型精度和刻画结果可靠性的核心挑战。论文从“非平稳解耦、数据纠偏与模型选择”的三元视角出发,系统梳理了场地土壤污染物三维刻画领域的关键问题与研究进展。首先,解析了非平稳性的形成机理及类型,探讨了非平稳性量化与解耦方法;其次,总结了稀疏有偏钻井数据的偏性来源及其纠偏方法体系,评估了不同模型对偏性数据的适应能力和改进方向;再次,比较了空间统计模型、机器学习模型与地球化学过程模型的优势与局限,剖析了三维刻画模型选择中存在的分歧与不确定性,提出在三元协同框架下构建多源数据驱动的高精度三维刻画体系的必要性。最后,结合智能决策技术的发展,展望了三维刻画技术在污染诊断、风险评估与可持续修复中的应用前景,强调了模型集成与动态优化在土壤污染治理中的潜在价值。研究为提升复杂场地土壤污染分布的三维刻画精度及数字化治理模式提供方法学参考。 展开更多
关键词 土壤污染物 三维分布 三元协同框架 数据纠偏 空间非平稳 模型选择
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体育教师何以卓越:中国香港卓越教学奖政策的演进历程、特征及启示
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作者 李笋南 李游 张少杰 《武汉体育学院学报》 北大核心 2025年第12期76-84,共9页
培养卓越体育教师、促进体育教师卓越化,由此确立卓越体育教师的专业形象并提升体育教师的专业地位,是我国内地和中国香港地区教育行政部门的共同愿景。自2003年起,中国香港体育教师卓越教学奖政策历经探索起步期(2003-2008年)、稳步推... 培养卓越体育教师、促进体育教师卓越化,由此确立卓越体育教师的专业形象并提升体育教师的专业地位,是我国内地和中国香港地区教育行政部门的共同愿景。自2003年起,中国香港体育教师卓越教学奖政策历经探索起步期(2003-2008年)、稳步推广期(2009-2018年)和蓬勃发展期(2019年至今),呈现一体两面、既肯定体育教师卓越教学表现又促进体育教师专业发展;紧扣体育学习领域课程改革要求、以卓越教学表现深化课程改革,与T-卓越计划并驾齐驱、共同促进体育教师卓越化等内涵特征。对我国内地体育教师卓越化的启示为:确立符合卓越之名的教师专业角色,塑造卓越体育教师的专业形象;优化保障卓越之途的专业发展路径,培育体育教师追求卓越的文化;研制凸显卓越之实的教师专业标准,构建体育教师卓越化的坐标系。 展开更多
关键词 卓越体育教师 卓越教学 体育教师专业发展 卓越体育教师标准 中国香港卓越教学奖
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Measurement of S-Wave Scattering Length between 6Li and 88Sr Atoms Using Interspecies Thermalization in an Optical Dipole Trap
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作者 Xiao-Bin Ma Zhu-Xiong Ye +3 位作者 li-Yang Xie Zhen Guo li you Meng Khoon Tey 《Chinese Physics Letters》 SCIE CAS CSCD 2019年第7期27-30,共4页
We report the creation of the first mixture of ^6Li and ^88Sr atoms in an optical dipole trap. Using this mixture,a measurement of the interspecies thermalization process is carried out and the previously unknown inte... We report the creation of the first mixture of ^6Li and ^88Sr atoms in an optical dipole trap. Using this mixture,a measurement of the interspecies thermalization process is carried out and the previously unknown interspecies s-wave scattering length between ^6Li and ^88Sr atoms is extracted to be |a6 Li-88 Sr|=(380-100^+160)a0 with a0 being the Bohr radius from the rate of interspecies thermalization. 展开更多
关键词 We report the creation OPTICAL DIPOLE trap
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Enhanced measurement precision with continuous interrogation during dynamical decoupling
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作者 张军 杜鹏 +3 位作者 敬雷 徐鹏 尤力 张文献 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第3期88-94,共7页
Dynamical decoupling(DD)is normally ineffective when applied to DC measurement.In its straightforward implementation,DD nulls out DC signal as well while suppressing noise.This work proposes a phase relay method that ... Dynamical decoupling(DD)is normally ineffective when applied to DC measurement.In its straightforward implementation,DD nulls out DC signal as well while suppressing noise.This work proposes a phase relay method that is capable of continuously interrogating the DC signal over many DD cycles.We illustrate its efficacy when applied to the measurement of a weak DC magnetic field with an atomic spinor Bose-Einstein condensate.Sensitivities approaching standard quantum limit or Heisenberg limit are potentially realizable for a coherent spin state or a squeezed spin state of 10000 atoms,respectively,while ambient laboratory level noise is suppressed by DD.Our work offers a practical approach to mitigate the limitations of DD to DC measurement and would find other applications for resorting coherence in quantum sensing and quantum information processing research. 展开更多
关键词 quantum sensing continuous interrogation quantum magnetometer dynamical decoupling Heisenberg limit
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OFDMA-Based Unsourced Random Access in LEO Satellite Internet of Things
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作者 Jiaqi Fang Gangle Sun +2 位作者 Wenjin Wang li you Rui Ding 《China Communications》 SCIE CSCD 2024年第1期13-23,共11页
This paper investigates the low earth orbit(LEO)satellite-enabled coded compressed sensing(CCS)unsourced random access(URA)in orthogonal frequency division multiple access(OFDMA)framework,where a massive uniform plana... This paper investigates the low earth orbit(LEO)satellite-enabled coded compressed sensing(CCS)unsourced random access(URA)in orthogonal frequency division multiple access(OFDMA)framework,where a massive uniform planar array(UPA)is equipped on the satellite.In LEO satellite communications,unavoidable timing and frequency offsets cause phase shifts in the transmitted signals,substantially diminishing the decoding performance of current terrestrial CCS URA receiver.To cope with this issue,we expand the inner codebook with predefined timing and frequency offsets and formulate the inner decoding as a tractable compressed sensing(CS)problem.Additionally,we leverage the inherent sparsity of the UPA-equipped LEO satellite angular domain channels,thereby enabling the outer decoder to support more active devices.Furthermore,the outputs of the outer decoder are used to reduce the search space of the inner decoder,which cuts down the computational complexity and accelerates the convergence of the inner decoding.Simulation results verify the effectiveness of the proposed scheme. 展开更多
关键词 LEO OFDMA SATELLITE unsourced random access UPA
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LINC00943靶向调控miR-125a-5p对幽门螺杆菌诱导的人胃黏膜上皮细胞GES-1损伤的影响
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作者 朱丽君 李伟智 +2 位作者 李游 唐瑜敏 雷青梅 《中华医院感染学杂志》 CAS CSCD 北大核心 2023年第2期198-202,共5页
目的探讨LINC00943对幽门螺杆菌(Hp)诱导的人胃黏膜上皮细胞GES-1损伤的影响及其分子机制。方法将GES-1细胞分为Control组、Hp组、Hp+si-NC组、Hp+si-LINC00943组、Hp+miR-NC组、Hp+miR-125a-5p组、Hp+si-LINC00943+anti-miR-NC组和Hp+s... 目的探讨LINC00943对幽门螺杆菌(Hp)诱导的人胃黏膜上皮细胞GES-1损伤的影响及其分子机制。方法将GES-1细胞分为Control组、Hp组、Hp+si-NC组、Hp+si-LINC00943组、Hp+miR-NC组、Hp+miR-125a-5p组、Hp+si-LINC00943+anti-miR-NC组和Hp+si-LINC00943+anti-miR-125a-5p组,实时荧光定量PCR检测LINC00943和miR-125a-5p表达水平;流式细胞术检测细胞凋亡;免疫蛋白印迹检测凋亡蛋白表达;酶联免疫吸附法检测白细胞介素-8(IL-8)和IL-1β、乳酸脱氢酶(LDH)水平;双荧光素酶报告基因实验检测LINC00943和miR-125a-5p靶向关系。结果与Control组比较,Hp组LINC00943表达、凋亡率、Bax蛋白、IL-8、IL-1β、LDH升高,miR-125a-5p表达、Bcl-2蛋白降低(P<0.05);敲减LINC00943或过表达miR-125a-5p可减轻Hp诱导的GES-1细胞损伤;LINC00943靶向调控miR-125a-5p表达,下调miR-125a-5p逆转了敲减LINC00943对Hp诱导的GES-1细胞损伤的影响(P<0.05)。结论敲减LINC00943通过靶向上调miR-125a-5p可缓解Hp诱导的GES-1细胞损伤。 展开更多
关键词 LINC00943 miR-125a-5p 幽门螺杆菌 人胃黏膜上皮细胞GES-1 细胞损伤
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PD-like finite time controller with simple structure for satellite attitude control
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作者 li you Ye Dong Xiao Bing 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2023年第8期123-133,共11页
A finite time controller with PD-like structure for satellite attitude control is proposed in this paper.The controller is constructed with simple structure based on standard PD controller.The fractional order term is... A finite time controller with PD-like structure for satellite attitude control is proposed in this paper.The controller is constructed with simple structure based on standard PD controller.The fractional order term is designed hence system could both have strong robustness and finite time convergence rate,and the advantage of finite time control and PD control is combined in this paper.System convergence rate is discussed by Lyapunov method,and the constraint on control parameters is given by implementing the coupled term of angular velocity and attitude quaternion.Moreover,the accuracy at steady stage depending on control parameters is given hence system could converge to this field within finite time.System stability and performance is demonstrated by numerical simulation results. 展开更多
关键词 Finite time control PD-like Simple structure Attitude control
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Erratum:Measurement of S-Wave Scattering Length between 6Li and 88Sr Atoms Using Interspecies Thermalization in an Optical Dipole Trap[Chin.Phys.Lett.36(2019)073401]
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作者 Xiao-Bin Ma Zhu-Xiong Ye +3 位作者 li-Yang Xie Zhen Guo li you Meng Khoon Tey 《Chinese Physics Letters》 SCIE CAS CSCD 2019年第10期99-99,共1页
In our previous report[Chin.Phys.Lett.36(2019)073401],the angular trap frequencies cji and 5 in Eq.(2)were wrongly substituted with trap frequencies(without 2π)at the computation stage.This results in underestimation... In our previous report[Chin.Phys.Lett.36(2019)073401],the angular trap frequencies cji and 5 in Eq.(2)were wrongly substituted with trap frequencies(without 2π)at the computation stage.This results in underestimation of the atom densities and thereby overestimation of the s-wave scatteting length between 6Li and 88Sr atoms. 展开更多
关键词 estimation substituted thereby
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Mechanism and Distribution of Calcareous Interbeds in Songtao Uplift and Its Periphery of Qiongdongnan Basin
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作者 Peiyuan Zhu li you +2 位作者 Qingtao Yuan Jia Zhong Aiqun liu 《Open Journal of Marine Science》 2018年第3期370-385,共16页
Based on core and casting slice observation, well drilling and logging data, the source of carbonate materials, lithology together with electric properties, types, genesis mechanism and distribution of calcareous inte... Based on core and casting slice observation, well drilling and logging data, the source of carbonate materials, lithology together with electric properties, types, genesis mechanism and distribution of calcareous interbeds in Songtao uplift and its periphery of Qiongdongnan Basin have been thoroughly analyzed. Results show that typical features have been appeared from well logging curves, containing low gamma-ray, low acoustic travel time, low neutron value, high density, as well as bright white calcium nodules or bands in electrical imaging well-logging curves. Drilling results reveal that calcareous interbeds developed mostly in high position of paleostructures and their distribution was controlled by the combined effects of macroscopic and microscopic factors. Macroscopically, calcareous interbeds relate to paleogeomorphology together with the combination of sandstone and mudstone. They are also controlled microscopically by the source of carbonate cements and pore space. Under normal circumstances, with regard to the same sand, the closer to the mudstone and the thicker of mudstone, the more conducive to the formation of calcareous interlayer. Low compaction strength, high content of rigid particles, coarse grain size, well-sorted sandstone and large pore space during carbonate cementation are favorable for the development of calcareous interbeds. 展开更多
关键词 CALCAREOUS Interbeds Material Sources Genetic MECHANISM DISTRIBUTION Rules Pore Space The Songtao UPLIFT and ITS PERIPHERY
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老年胃肠肿瘤手术患者术后谵妄的发生率和危险因素:单中心观察性研究
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作者 马勇 方仕旭 +2 位作者 李由 曹万龙 李洲 《肿瘤预防与治疗》 2025年第10期905-910,共6页
目的:探讨老年胃肠肿瘤手术患者术后谵妄(postoperative delirium,POD)的发生率和危险因素。方法:连续纳入自贡市第一人民医院普外科2021年1月至2023年6月期间收治的402例择期行腹腔镜胃肠肿瘤手术的老年患者作为研究对象,采用意识模糊... 目的:探讨老年胃肠肿瘤手术患者术后谵妄(postoperative delirium,POD)的发生率和危险因素。方法:连续纳入自贡市第一人民医院普外科2021年1月至2023年6月期间收治的402例择期行腹腔镜胃肠肿瘤手术的老年患者作为研究对象,采用意识模糊评分法(Confusion Assessment Method,CAM)每日2次评估术后7d内POD的发生情况,计算POD的发生率,并按照是否发生POD分为2组,采用Logistic回归模型分析老年腹腔镜胃肠肿瘤手术患者术后发生POD的危险因素。结果:纳入402例老年腹腔镜胃肠肿瘤手术患者,有70例术后发生POD,发生率为17.41%,发生时间在术后的第1~7d,其中,85.71%的POD发生在术后第2~5 d。多因素Logistic回归分析显示:年龄(OR=1.159,95%CI:1.063~1.263,P=0.001)、酗酒(OR=5.590,95%CI:1.843~19.212,P=0.003)、糖尿病(OR=5.103,95%CI:1.817~14.333,P=0.002)、术前血红蛋白降低(OR=0.097,95%CI:0.952~0.996,P=0.019)、术后首次下床活动时间(OR=2.235,95%CI:1.779~2.808,P<0.001)、术后住院时间(OR=1.922,95%CI:1.440~2.566,P<0.001)是老年腹腔镜胃肠肿瘤手术患者术后发生POD的独立危险因素。结论:高龄、酗酒、糖尿病、术前低血红蛋白水平降低、术后首次下床活动时间延长、术后住院时间延长是老年腹腔镜胃肠肿瘤手术患者术后发生POD的独立危险因素。 展开更多
关键词 胃肠肿瘤 老年患者 术后谵妄 危险因素 LOGISTIC回归模型
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