A dual-phase synergistic enhancement method was adopted to strengthen the Al-Mn-Mg-Sc-Zr alloy fabricated by laser powder bed fusion(LPBF)by leveraging the unique advantages of Er and TiB_(2).Spherical powders of 0.5w...A dual-phase synergistic enhancement method was adopted to strengthen the Al-Mn-Mg-Sc-Zr alloy fabricated by laser powder bed fusion(LPBF)by leveraging the unique advantages of Er and TiB_(2).Spherical powders of 0.5wt%Er-1wt%TiB_(2)/Al-Mn-Mg-Sc-Zr nanocomposite were prepared using vacuum homogenization technique,and the density of samples prepared through the LPBF process reached 99.8%.The strengthening and toughening mechanisms of Er-TiB_(2)were investigated.The results show that Al_(3)Er diffraction peaks are detected by X-ray diffraction analysis,and texture strength decreases according to electron backscatter diffraction results.The added Er and TiB_(2)nano-reinforcing phases act as heterogeneous nucleation sites during the LPBF forming process,hindering grain growth and effectively refining the grains.After incorporating the Er-TiB_(2)dual-phase nano-reinforcing phases,the tensile strength and elongation at break of the LPBF-deposited samples reach 550 MPa and 18.7%,which are 13.4%and 26.4%higher than those of the matrix material,respectively.展开更多
To overcome the shortage of complex equipment,large volume,and high energy consumption in space capsule manufacturing,a novel sliding pressure Joule heat fuse additive manufacturing technique with reduced volume and l...To overcome the shortage of complex equipment,large volume,and high energy consumption in space capsule manufacturing,a novel sliding pressure Joule heat fuse additive manufacturing technique with reduced volume and low energy consumption was proposed.But the unreasonable process parameters may lead to the inferior consistency of the forming quality of single-channel multilayer in Joule heat additive manufacturing process,and it is difficult to reach the condition for forming thinwalled parts.Orthogonal experiments were designed to fabricate single-channel multilayer samples with varying numbers of layers,and their forming quality was evaluated.The influence of printing current,forming speed,and contact pressure on the forming quality of the single-channel multilayer was analyzed.The optimal process parameters were obtained and the quality characterization of the experiment results was conducted.Results show that the printing current has the most significant influence on the forming quality of the single-channel multilayer.Under the optimal process parameters,the forming section is well fused and the surface is continuously smooth.The surface roughness of a single-channel 3-layer sample is 0.16μm,and the average Vickers hardness of cross section fusion zone is 317 HV,which lays a foundation for the subsequent use of Joule heat additive manufacturing technique to form thinwall parts.展开更多
目的通过同位素标记的相对和绝对定量(isobaric tag for relative and absolute quantitation,iTRAQ)蛋白质组学技术探讨Graves病(Graves′disease,GD)患者血清蛋白质组学变化,为自身免疫性甲状腺疾病的预防及临床筛查提供新的生物标志...目的通过同位素标记的相对和绝对定量(isobaric tag for relative and absolute quantitation,iTRAQ)蛋白质组学技术探讨Graves病(Graves′disease,GD)患者血清蛋白质组学变化,为自身免疫性甲状腺疾病的预防及临床筛查提供新的生物标志物。方法收集9例Graves病患者、14例对照组受试者的外周血清标本。应用相对和绝对定量同位素标记的蛋白质组学(iTRAQ)技术联合液相色谱法以及串联质谱分析对血清蛋白质进行鉴定及相对定量分析。结果Graves病组中共鉴定出差异蛋白41个,其中上调蛋白有12个、下调蛋白29个。生物信息功能分析提示这些差异蛋白主要参与细胞凋亡、细胞的黏附、炎症反应、补体激活、细胞氧化应激、凝血等过程,以上差异蛋白中有9个显著差异蛋白可作为候选蛋白,分别是上调蛋白血管性血友病因子(von Willebrand factor,vWF)、铜蓝蛋白(ceruloplasmin,CP)、性激素结合蛋白(sex hormone-binding globulin,SHBG)、波形蛋白(vimentin,VIM)和下调蛋白纤溶酶原(plasminogen,PLG)、(clusterin,CLU)、血管紧张素原(angiotensinogen,AGT)、载脂蛋白A-II(apolipoprotein A-II,APOA2)、生物素酶(biotinidase,BTD)。结论这9种蛋白在Graves病过程中发挥重要作用,可能在Graves病临床筛查工作中起到关键生物标志物作用。基于iTRAQ技术的蛋白质组学方法有助于鉴定出Graves病相关的差异蛋白质,为进一步探索Graves病标志物提供了新的思路和线索。展开更多
基金Shaanxi Province Qin Chuangyuan“Scientist+Engineer”Team Construction Project(2022KXJ-071)2022 Qin Chuangyuan Achievement Transformation Incubation Capacity Improvement Project(2022JH-ZHFHTS-0012)+8 种基金Shaanxi Province Key Research and Development Plan-“Two Chains”Integration Key Project-Qin Chuangyuan General Window Industrial Cluster Project(2023QCY-LL-02)Xixian New Area Science and Technology Plan(2022-YXYJ-003,2022-XXCY-010)2024 Scientific Research Project of Shaanxi National Defense Industry Vocational and Technical College(Gfy24-07)Shaanxi Vocational and Technical Education Association 2024 Vocational Education Teaching Reform Research Topic(2024SZX354)National Natural Science Foundation of China(U24A20115)2024 Shaanxi Provincial Education Department Service Local Special Scientific Research Program Project-Industrialization Cultivation Project(24JC005,24JC063)Shaanxi Province“14th Five-Year Plan”Education Science Plan,2024 Project(SGH24Y3181)National Key Research and Development Program of China(2023YFB4606400)Longmen Laboratory Frontier Exploration Topics Project(LMQYTSKT003)。
文摘A dual-phase synergistic enhancement method was adopted to strengthen the Al-Mn-Mg-Sc-Zr alloy fabricated by laser powder bed fusion(LPBF)by leveraging the unique advantages of Er and TiB_(2).Spherical powders of 0.5wt%Er-1wt%TiB_(2)/Al-Mn-Mg-Sc-Zr nanocomposite were prepared using vacuum homogenization technique,and the density of samples prepared through the LPBF process reached 99.8%.The strengthening and toughening mechanisms of Er-TiB_(2)were investigated.The results show that Al_(3)Er diffraction peaks are detected by X-ray diffraction analysis,and texture strength decreases according to electron backscatter diffraction results.The added Er and TiB_(2)nano-reinforcing phases act as heterogeneous nucleation sites during the LPBF forming process,hindering grain growth and effectively refining the grains.After incorporating the Er-TiB_(2)dual-phase nano-reinforcing phases,the tensile strength and elongation at break of the LPBF-deposited samples reach 550 MPa and 18.7%,which are 13.4%and 26.4%higher than those of the matrix material,respectively.
基金Shaanxi Province Qin Chuangyuan“Scientist+Engineer”Team Construction Project(2022KXJ-071)2022 Qin Chuangyuan Achievement Transformation Incubation Capacity Improvement Project(2022JH-ZHFHTS-0012)+1 种基金Shaanxi Province Key Research and Development Plan-“Two Chains”Integration Key Project-Qin Chuangyuan General Window Industrial Cluster Project(2023QCY-LL-02)Xixian New Area Science and Technology Plan(2022-YXYJ-003,2022-XXCY-010)。
文摘To overcome the shortage of complex equipment,large volume,and high energy consumption in space capsule manufacturing,a novel sliding pressure Joule heat fuse additive manufacturing technique with reduced volume and low energy consumption was proposed.But the unreasonable process parameters may lead to the inferior consistency of the forming quality of single-channel multilayer in Joule heat additive manufacturing process,and it is difficult to reach the condition for forming thinwalled parts.Orthogonal experiments were designed to fabricate single-channel multilayer samples with varying numbers of layers,and their forming quality was evaluated.The influence of printing current,forming speed,and contact pressure on the forming quality of the single-channel multilayer was analyzed.The optimal process parameters were obtained and the quality characterization of the experiment results was conducted.Results show that the printing current has the most significant influence on the forming quality of the single-channel multilayer.Under the optimal process parameters,the forming section is well fused and the surface is continuously smooth.The surface roughness of a single-channel 3-layer sample is 0.16μm,and the average Vickers hardness of cross section fusion zone is 317 HV,which lays a foundation for the subsequent use of Joule heat additive manufacturing technique to form thinwall parts.
文摘目的通过同位素标记的相对和绝对定量(isobaric tag for relative and absolute quantitation,iTRAQ)蛋白质组学技术探讨Graves病(Graves′disease,GD)患者血清蛋白质组学变化,为自身免疫性甲状腺疾病的预防及临床筛查提供新的生物标志物。方法收集9例Graves病患者、14例对照组受试者的外周血清标本。应用相对和绝对定量同位素标记的蛋白质组学(iTRAQ)技术联合液相色谱法以及串联质谱分析对血清蛋白质进行鉴定及相对定量分析。结果Graves病组中共鉴定出差异蛋白41个,其中上调蛋白有12个、下调蛋白29个。生物信息功能分析提示这些差异蛋白主要参与细胞凋亡、细胞的黏附、炎症反应、补体激活、细胞氧化应激、凝血等过程,以上差异蛋白中有9个显著差异蛋白可作为候选蛋白,分别是上调蛋白血管性血友病因子(von Willebrand factor,vWF)、铜蓝蛋白(ceruloplasmin,CP)、性激素结合蛋白(sex hormone-binding globulin,SHBG)、波形蛋白(vimentin,VIM)和下调蛋白纤溶酶原(plasminogen,PLG)、(clusterin,CLU)、血管紧张素原(angiotensinogen,AGT)、载脂蛋白A-II(apolipoprotein A-II,APOA2)、生物素酶(biotinidase,BTD)。结论这9种蛋白在Graves病过程中发挥重要作用,可能在Graves病临床筛查工作中起到关键生物标志物作用。基于iTRAQ技术的蛋白质组学方法有助于鉴定出Graves病相关的差异蛋白质,为进一步探索Graves病标志物提供了新的思路和线索。
