OBJECTIVE: To investigate the effect and underlying mechanisms of combined medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine on antioxidant levels in a rat model of sciati...OBJECTIVE: To investigate the effect and underlying mechanisms of combined medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine on antioxidant levels in a rat model of sciatica.METHODS: One hundred Wistar rats, of specific pathogen free level, were randomly divided into five groups: normal control group, model group,medicated thread moxibustion group, needle picking group, and combination group. Each group contained 20 rats. In the model, medicated thread moxibustion, needle picking, and combination groups,sciatica models were established through chronicconstriction injury of the sciatic nerve. After the model was established, the rats in the medicated thread moxibustion, needle picking, and combination groups were given the corresponding therapies for 21 days. The control and model groups received no treatment. Reactive oxygen species, superoxide dismutase, malondialdehyde, and total antioxidant capacity changes were determined.Nicotinamide adenine dinucleotide phosphate(NADPH) oxidase subunit NADPH oxidases 4(NOX4) m RNA expression and the morphology of cells were observed to detect apoptosis of gamma-aminobutyric acid ergic(GABAergic) neurons.RESULTS: Compared with control group, reactive oxygen species and malondialdehyde levels rose significantly in the model group(P < 0.01), while superoxide dismutase and total antioxidant capacity levels were lowered(P < 0.05). Compared with the model group, reactive oxygen species and malondialdehyde decreased in the needle picking group(P < 0.05), while superoxide dismutase levels were increased(P < 0.05); reactive oxygen species and malondialdehyde significantly decreased in the combination group(P < 0.01). In addition, the model group had higher NOX4 m RNA expression than that of the control group(P < 0.05), and the combination group had lower expression levels than that of the model group(P < 0.05). Apoptosis of GABAergic neurons was observed in the model group, and was attenuated after combined therapy.CONCLUSION: The medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine can prevent oxidative damage in the rat model of sciatica via down-regulating NOX4 expression, improving antioxidant capacity, and in-hibiting the oxidative damage pathway of GABAergic neurons.展开更多
目的探讨桃仁承气汤化裁联合西医序贯治疗对2型糖尿病(T2DM)患者中医证候积分及血糖波动、血清白脂素(Asprosin)水平的影响。方法选取90例T2DM患者,并将其随机分成序贯组(n=45)和中医组(n=45)。序贯组44例(脱落1例)先开展胰岛素强化治疗...目的探讨桃仁承气汤化裁联合西医序贯治疗对2型糖尿病(T2DM)患者中医证候积分及血糖波动、血清白脂素(Asprosin)水平的影响。方法选取90例T2DM患者,并将其随机分成序贯组(n=45)和中医组(n=45)。序贯组44例(脱落1例)先开展胰岛素强化治疗,之后改用度拉糖肽治疗;中医组44例(脱落1例)在序贯组基础上加用桃仁承气汤化裁方治疗。比较2组治疗前、治疗8周后中医证候积分、血糖波动参数及空腹血糖(FPG)、胰岛功能指标及血清Asprosin水平。结果治疗8周后,2组中医证候积分、血糖标准差(SDBG)、平均血糖波动幅度(MAGE)及FPG、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、胰高血糖素、空腹和餐后2 h C肽、血清Asprosin水平与治疗前比较,差异有统计学意义(P<0.05);中医组除SDBG以外的其他指标水平与序贯组比较,差异有统计学意义(P<0.05)。结论桃仁承气汤化裁方联合西医序贯治疗可明显下调T2DM患者血清Asprosin水平及中医证候积分,有效控制血糖波动,显著改善胰岛功能。展开更多
基金Supported by the National Natural Science Foundation of China(Research on Mechanism of Insomnia Treatment Administrated by Acupuncture Combined With Medicated Thread Moxibustion of Zhuang Nationality Medicine,No.81360579)
文摘OBJECTIVE: To investigate the effect and underlying mechanisms of combined medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine on antioxidant levels in a rat model of sciatica.METHODS: One hundred Wistar rats, of specific pathogen free level, were randomly divided into five groups: normal control group, model group,medicated thread moxibustion group, needle picking group, and combination group. Each group contained 20 rats. In the model, medicated thread moxibustion, needle picking, and combination groups,sciatica models were established through chronicconstriction injury of the sciatic nerve. After the model was established, the rats in the medicated thread moxibustion, needle picking, and combination groups were given the corresponding therapies for 21 days. The control and model groups received no treatment. Reactive oxygen species, superoxide dismutase, malondialdehyde, and total antioxidant capacity changes were determined.Nicotinamide adenine dinucleotide phosphate(NADPH) oxidase subunit NADPH oxidases 4(NOX4) m RNA expression and the morphology of cells were observed to detect apoptosis of gamma-aminobutyric acid ergic(GABAergic) neurons.RESULTS: Compared with control group, reactive oxygen species and malondialdehyde levels rose significantly in the model group(P < 0.01), while superoxide dismutase and total antioxidant capacity levels were lowered(P < 0.05). Compared with the model group, reactive oxygen species and malondialdehyde decreased in the needle picking group(P < 0.05), while superoxide dismutase levels were increased(P < 0.05); reactive oxygen species and malondialdehyde significantly decreased in the combination group(P < 0.01). In addition, the model group had higher NOX4 m RNA expression than that of the control group(P < 0.05), and the combination group had lower expression levels than that of the model group(P < 0.05). Apoptosis of GABAergic neurons was observed in the model group, and was attenuated after combined therapy.CONCLUSION: The medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine can prevent oxidative damage in the rat model of sciatica via down-regulating NOX4 expression, improving antioxidant capacity, and in-hibiting the oxidative damage pathway of GABAergic neurons.
文摘目的探讨桃仁承气汤化裁联合西医序贯治疗对2型糖尿病(T2DM)患者中医证候积分及血糖波动、血清白脂素(Asprosin)水平的影响。方法选取90例T2DM患者,并将其随机分成序贯组(n=45)和中医组(n=45)。序贯组44例(脱落1例)先开展胰岛素强化治疗,之后改用度拉糖肽治疗;中医组44例(脱落1例)在序贯组基础上加用桃仁承气汤化裁方治疗。比较2组治疗前、治疗8周后中医证候积分、血糖波动参数及空腹血糖(FPG)、胰岛功能指标及血清Asprosin水平。结果治疗8周后,2组中医证候积分、血糖标准差(SDBG)、平均血糖波动幅度(MAGE)及FPG、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、胰高血糖素、空腹和餐后2 h C肽、血清Asprosin水平与治疗前比较,差异有统计学意义(P<0.05);中医组除SDBG以外的其他指标水平与序贯组比较,差异有统计学意义(P<0.05)。结论桃仁承气汤化裁方联合西医序贯治疗可明显下调T2DM患者血清Asprosin水平及中医证候积分,有效控制血糖波动,显著改善胰岛功能。
