AIM To establish a new improved vascularanastomotic technique to simplify the surgicaltechnique and increase the survival rate of smallintestinal transplantation in rats.METHODS The graft removed en blocconsisted of e...AIM To establish a new improved vascularanastomotic technique to simplify the surgicaltechnique and increase the survival rate of smallintestinal transplantation in rats.METHODS The graft removed en blocconsisted of entire small intestine,portal veinand aortic segment with superior mesentericartery.The graft was perfused in situ and thegut lumen was irrigated during the operation.Heterotopic small bowel transplantation wasperformed by microvascular end-to-sideanastomosis between the donor aortic segmentwith superior mesenteric artery and the recipientabdominal aorta,and by the formation of a'Cuff'anastomosis between the donor portalvein and the recipient left renal vein.Both endsof the grafts were exteriorized as stomas.RESULTS A total of 189 intestinaltransplantations were performed in rats,33 ofwhich were involved in the formal experimentalgroup,with a survival rate of 84.8%.Theaverage time for the donor surgery was 80min±10min;for graft repair 10min±3min;and forrecipient surgery 95min±15min.The averagetime for the arterial anastomosis and the veinanastomosis was 18min±5min and 1min,respectively.The warm ischemic time and coldischemic time were 22min±5min and less than60min,respectively.The whole operation wascompleted by a single surgeon,the operativetime being about 3 hours.CONCLUSION The vascular anastomosis used in this study could simplify surgical technique,reduce the operative time and elevate thesurvival rate of small intestinal transplantationin rats.展开更多
AIM To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage. METHODS The swine segmental small bowel graft was autotransplanted after it was preserved with ...AIM To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage. METHODS The swine segmental small bowel graft was autotransplanted after it was preserved with lactated Ringer′s (LR), Euro Collins (EC), hyperosmolarity citrate adenine (HC A) and WMO 1 solutions for 10, 18 and 24 hours, respectively. The recipient survival rate, morphological structure, graft mucosal energy substances and Na + K + ATPase activity were studied, and graft absorption was estimated with D xylose absorption test. RESULTS The morphological study of the grafts preserved with LR or HC A solution for 10 hours or with EC and WMO 1 solution for 18 hours was normal 6 days after operation. Mucosal ATP, total adenine nucleotides (TAN) contents and Na + K + ATPase activity of the graft preserved with EC or WMO solution were higher than that of the graft preserved with LR or HC A solution. Serum level of D xylose was higher in EC and WMO 1 groups than in LR and HC A groups when the graft was preserved for 24 hours. CONCLUSIONS EC and WMO 1 solutions can preserve the swine small bowel up to 18 hours, which are superior to LR and HC A solutions.展开更多
INTRODUCTIONThe use of combined pancreatic and renaltransplantation in patients with diabetes and end-stage renal failure has gained widespreadrecognition as an acceptable treatment option.Theprevailing method used fo...INTRODUCTIONThe use of combined pancreatic and renaltransplantation in patients with diabetes and end-stage renal failure has gained widespreadrecognition as an acceptable treatment option.Theprevailing method used for transplantation of thepancreas involves anastomosis of the graft’s portalvein and the rccipient’s iliac vein to provide展开更多
基金the 9.5 Military Medical Research Fund of China,No.96M021
文摘AIM To establish a new improved vascularanastomotic technique to simplify the surgicaltechnique and increase the survival rate of smallintestinal transplantation in rats.METHODS The graft removed en blocconsisted of entire small intestine,portal veinand aortic segment with superior mesentericartery.The graft was perfused in situ and thegut lumen was irrigated during the operation.Heterotopic small bowel transplantation wasperformed by microvascular end-to-sideanastomosis between the donor aortic segmentwith superior mesenteric artery and the recipientabdominal aorta,and by the formation of a'Cuff'anastomosis between the donor portalvein and the recipient left renal vein.Both endsof the grafts were exteriorized as stomas.RESULTS A total of 189 intestinaltransplantations were performed in rats,33 ofwhich were involved in the formal experimentalgroup,with a survival rate of 84.8%.Theaverage time for the donor surgery was 80min±10min;for graft repair 10min±3min;and forrecipient surgery 95min±15min.The averagetime for the arterial anastomosis and the veinanastomosis was 18min±5min and 1min,respectively.The warm ischemic time and coldischemic time were 22min±5min and less than60min,respectively.The whole operation wascompleted by a single surgeon,the operativetime being about 3 hours.CONCLUSION The vascular anastomosis used in this study could simplify surgical technique,reduce the operative time and elevate thesurvival rate of small intestinal transplantationin rats.
文摘AIM To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage. METHODS The swine segmental small bowel graft was autotransplanted after it was preserved with lactated Ringer′s (LR), Euro Collins (EC), hyperosmolarity citrate adenine (HC A) and WMO 1 solutions for 10, 18 and 24 hours, respectively. The recipient survival rate, morphological structure, graft mucosal energy substances and Na + K + ATPase activity were studied, and graft absorption was estimated with D xylose absorption test. RESULTS The morphological study of the grafts preserved with LR or HC A solution for 10 hours or with EC and WMO 1 solution for 18 hours was normal 6 days after operation. Mucosal ATP, total adenine nucleotides (TAN) contents and Na + K + ATPase activity of the graft preserved with EC or WMO solution were higher than that of the graft preserved with LR or HC A solution. Serum level of D xylose was higher in EC and WMO 1 groups than in LR and HC A groups when the graft was preserved for 24 hours. CONCLUSIONS EC and WMO 1 solutions can preserve the swine small bowel up to 18 hours, which are superior to LR and HC A solutions.
基金a grant from the Army Medical Science and Technology Research,No.96Z002
文摘INTRODUCTIONThe use of combined pancreatic and renaltransplantation in patients with diabetes and end-stage renal failure has gained widespreadrecognition as an acceptable treatment option.Theprevailing method used for transplantation of thepancreas involves anastomosis of the graft’s portalvein and the rccipient’s iliac vein to provide
