In our recent studies,we found that LMP1 encoded by Epstein-Barr virus could accelerate the formation of active c-Jun/Jun B heterodimer.We studied the regulation of cyclinD1 by c-Jun/Jun B heterodimers by laser scanni...In our recent studies,we found that LMP1 encoded by Epstein-Barr virus could accelerate the formation of active c-Jun/Jun B heterodimer.We studied the regulation of cyclinD1 by c-Jun/Jun B heterodimers by laser scanning confocal influorescence microscopy,Western blot,luciferase activity assay,super-EMSA and flow cytometry in the Tet-on-LMP1 HNE2 cell line,in which LMP1 expression was regulated by Tet-on system.c-Jun/Jun B heterodimers induced by LMP1 could up regulate cyclin D1 promoter activity and expression.Overexpression of cy-clinD1 accelerated the progression of cell cycle.展开更多
基金This work was supported by the StateKey Basic Research Program,Foundational Investigation on Human Carcinogenes is of China(Grant No.G1998051201)National Natural Science Foundat ion for Distinguished Young Scholars of China(Grant No.39525022)National Natural Science Foundation of China(Grant Nos.30300403,30100005&30000087).
文摘In our recent studies,we found that LMP1 encoded by Epstein-Barr virus could accelerate the formation of active c-Jun/Jun B heterodimer.We studied the regulation of cyclinD1 by c-Jun/Jun B heterodimers by laser scanning confocal influorescence microscopy,Western blot,luciferase activity assay,super-EMSA and flow cytometry in the Tet-on-LMP1 HNE2 cell line,in which LMP1 expression was regulated by Tet-on system.c-Jun/Jun B heterodimers induced by LMP1 could up regulate cyclin D1 promoter activity and expression.Overexpression of cy-clinD1 accelerated the progression of cell cycle.
