Since inferior biomimetic fabrication and anti-thrombosis process always lead to the implantation failure of artificial vascular grafts,the development of bioactive platform which can promote endothelialization is fun...Since inferior biomimetic fabrication and anti-thrombosis process always lead to the implantation failure of artificial vascular grafts,the development of bioactive platform which can promote endothelialization is fundamental in the research for blood vessel substitutes to overcome the low-efficiency or hyperplasia.To solve the limitation of insufficient autologous sources of small blood vessels,this study prepared a tri-layer small diameter vascular based on citrate,followed by covalently immobilizing of specific peptides to achieve in situ cells enrichment.The natural carbohydrate of carboxymethyl chitosan is creatively used as the inner layer of tubular graft,thus exerting the anti-thrombosis effect.Moreover,the gastrodin coating was employed to alleviate the inflammation response and increase the safety of this surgically implanted vascular scaffold.The synthesis process was characterized,its biocompatibility,hierarchically cell induction capacity,anticoagulant effect and the ability to promote neovascularization were evaluated.In such way,we hope to prepare a biodegradable small-diameter artificial vascular for the clinical application.展开更多
基金supported by the National Natural Science Foundation of China(grant NO.82260088/81760087)Major Program of Kunming Science and Technology Innovation Center(grant NO.2019-1-N-25318000003568)+3 种基金the Applied Basic Research Foundation of Yunnan Province(grant NO.202201AY070001-029/202201AY070001-030/202001AY070001-014/202401AY070001-030)First-Class Discipline Team of Kunming Medical University(2024XKTDYS01)Yunnan Pro-vincial Innovative Research Team in Basic and Clinical Study of Coronary Heart Disease of Yunnan Revitalization Talent Support Program(202305AS350030)the Foundation of Expert Workstation of Bai Xiaochun(YSZJGZZ-2020040).
文摘Since inferior biomimetic fabrication and anti-thrombosis process always lead to the implantation failure of artificial vascular grafts,the development of bioactive platform which can promote endothelialization is fundamental in the research for blood vessel substitutes to overcome the low-efficiency or hyperplasia.To solve the limitation of insufficient autologous sources of small blood vessels,this study prepared a tri-layer small diameter vascular based on citrate,followed by covalently immobilizing of specific peptides to achieve in situ cells enrichment.The natural carbohydrate of carboxymethyl chitosan is creatively used as the inner layer of tubular graft,thus exerting the anti-thrombosis effect.Moreover,the gastrodin coating was employed to alleviate the inflammation response and increase the safety of this surgically implanted vascular scaffold.The synthesis process was characterized,its biocompatibility,hierarchically cell induction capacity,anticoagulant effect and the ability to promote neovascularization were evaluated.In such way,we hope to prepare a biodegradable small-diameter artificial vascular for the clinical application.
