Using coal gangue(CG)as raw material,a new type of all solid-waste-based 13-X molecular sieve material was controllably prepared by alkali fusion-hydrothermal method.The synthetic molecular sieve was used as a solid a...Using coal gangue(CG)as raw material,a new type of all solid-waste-based 13-X molecular sieve material was controllably prepared by alkali fusion-hydrothermal method.The synthetic molecular sieve was used as a solid adsorbent to treat Cd^(2+)-containing wastewater,and its adsorption behavior on Cd^(2+)in aqueous solution was studied and analyzed.The microstructure and morphology of the molecular sieve were investigated by X-ray diffraction(XRD),field emission scanning electron microscopy(FESEM)and specific surface area analyzer.The results show that the synthesized 13-X molecular sieve has higher Brunauer–Emmett–Teller(BET)specific surface area with higher crystallinity and higher adsorption capacity for the heavy metal Cd^(2+).The adsorption process of Cd^(2+)by molecular sieve conforms to the Langmuir isotherm adsorption equation and Lagergren pseudo-second-order rate equation.Combined with thermodynamic calculation,it can be concluded that the adsorption process is physically monolayer,spontaneous and exothermic.In this study,a low-cost and naturally available synthesis method of 13-X molecular sieve is reported.Combined with its adsorption mechanism for Cd^(2+),it provides a feasible and general method for removing heavy metal ions from coal gangue and also provides a new way for the utilization of coal gangue with high added value.展开更多
Designing bifunctional oxygen reduction/evolution(ORR/OER)catalysts with high activity,robust stability and low cost is the key to accelerating the commercialization of rechargeable zinc-air battery(RZAB).Here,we prop...Designing bifunctional oxygen reduction/evolution(ORR/OER)catalysts with high activity,robust stability and low cost is the key to accelerating the commercialization of rechargeable zinc-air battery(RZAB).Here,we propose a template-assisted electrospinning strategy to in situ fabricate 3D fibers consisting of FeNi nanoparticles embedded into N-doped hollow porous carbon nanospheres(FeNi@NHCFs)as the stable binder-free integrated air cathode in RZAB.3D interconnected conductive fiber networks provide fast electron transfer pathways and strengthen the mechanical flexibility.Meanwhile,N-doped hollow porous carbon nanospheres not only evenly confine FeNi nanoparticles to provide sufficient catalytic active sites,but also endow optimum mass transfer environment to reduce diffusion barrier.The RZABs assembled by FeNi@NHCFs as integrated air cathodes exhibit outstanding battery performance with high open-circuit voltage,large discharge specific capacity and power density,durable cyclic stability and great flexibility.Thus,this work brings a useful strategy to fabricate the integrated electrodes without using any polymeric binders for metal air batteries and other related fields.展开更多
BACKGROUND Pancreatic cancer,characterized by aggressive proliferation and metastasis,is a lethal malignancy.The nightly hormone melatonin serves as a rhythm-regulating hormone,and is used to treat different cancers i...BACKGROUND Pancreatic cancer,characterized by aggressive proliferation and metastasis,is a lethal malignancy.The nightly hormone melatonin serves as a rhythm-regulating hormone,and is used to treat different cancers including pancreatic cancer.AIM To investigate how melatonin acts against human pancreatic cancer cell lines and analyze the biological processes that cause the observed effects.METHODS Panc-1 and AsPC-1 cells were treated with melatonin.Cell viability was measured using the cell counting kit-8 assay.Western blotting and immunofluorescence were used to analyze protein expression levels.Ferroptosis was measured by analyzing lipid reactive oxygen species and malondialdehyde levels;apoptosis was assessed using flow cytometry.RESULTS Melatonin significantly inhibited the viability,colony formation,migration,and invasion of Panc-1 and AsPC-1 cells.Additionally,melatonin activated the endoplasmic reticulum(ER)stress pathway(protein kinase R-like ER kinase eukaryotic initiation factor 2α-activating transcription factor 4),inhibited glutamine metabolism(alanine-serinecysteine transporter 2-glutaminase 1-glutathione peroxidase 4,alanine-serine-cysteine transporter 2-glutathione peroxidase 4),and promoted ferroptosis in pancreatic cancer cells.Co-treatment with a high melatonin concentration and protein kinase R-like ER kinase agonist(CCT020312)enhanced melatonin-induced ferroptosis in pancreatic cancer cells.Melatonin demonstrated a variety of anticancer effects by inhibiting autophagy.This was achieved through the increased expression of sequestosome-1 and decreased expression of light chain 3.Additionally,melatonin facilitated the promotion of apoptosis.CONCLUSION Melatonin induces ferroptosis in pancreatic cancer cells by activating transcription factor 4-dependent ER stress and inhibiting glutamine metabolism,promotes apoptosis in pancreatic cancer cells,and inhibits autophagy,leading to synergistic anticancer effects.展开更多
Nanoscale hierarchically porous metal–organic frameworks(NHP-MOFs)have received unprecedented attention in many fields owing to their integration of the strengths of nanoscale size(<1μm)and hierarchical porous st...Nanoscale hierarchically porous metal–organic frameworks(NHP-MOFs)have received unprecedented attention in many fields owing to their integration of the strengths of nanoscale size(<1μm)and hierarchical porous structure(micro-,meso-and/or macro-pores)of MOFs.This review focuses on recent advances in the main synthetic strategies for NHP-MOFs based on different metal ions(e.g.,Cu,Fe,Co,Zn,Al,Zr,and Cr),including the template method,composite technology,post-synthetic modification,in situ growth and the grind method.In addition,the mechanisms of synthesis,regulation techniques and the advantages and disadvantages of various methods are discussed.Finally,the challenges and prospects of the commercialisation of promising NHP-MOFs are also presented.The purpose of this review is to provide a road map for future design and development of NHP-MOFs for practical application.展开更多
PEGylated liposomes are potential candidates to improve the pharmacokinetic characteristics of encapsulated drugs, to extend their circulation half-life and facilitate their passive accumulation at tumour sites. Howev...PEGylated liposomes are potential candidates to improve the pharmacokinetic characteristics of encapsulated drugs, to extend their circulation half-life and facilitate their passive accumulation at tumour sites. However, PEG-modified liposomes can induce accelerated blood clearance(ABC) upon repeated administration, and the extent of ABC phenomenon on the cytotoxic drugs-containing PEGylated liposomes is related to the dose of the cytotoxic drugs.In this study, EPI served as a model cytotoxic drug, a hydrophilic surfactant molecule,monosialylganglioside(GM1) was chosen and modified on the liposomes together with PEG.It was shown that upon mixed modification, when GM1 contents reached 10% or 15% mol,the ABC phenomenon of the PEGylated liposomal EPI significantly reduced. We also found that GM1 played an important role in abrogating the ABC phenomenon in both the induction phase and the effectuation phase. The results suggested that GM1 incorporation unfortunately did not avoid occurrence of ABC phenomenon completely, but GM1 modification on PEGylated liposomes may provide a significant improvement in clinical practice of PEGylated liposomes. Further study must be necessary.展开更多
A novel Fe/Co metal organic complex nanosheet modified by 1,4-dicarboxybenzene(BDC),i.e.,FeCo@BDC,was prepared,and highly efficient removal performance for trace lead(Ⅱ)(Pb^(2+))was demonstrated in the neutral aqueou...A novel Fe/Co metal organic complex nanosheet modified by 1,4-dicarboxybenzene(BDC),i.e.,FeCo@BDC,was prepared,and highly efficient removal performance for trace lead(Ⅱ)(Pb^(2+))was demonstrated in the neutral aqueous solutions.The removal rates were higher than 95%and the adsorption was equilibrated in 15 min.The isotherms and kinetics for the adsorption Pb^(2+)by the FeCo@BDC adsorbents followed Langmuir model and pseudo-second-order model,respectively.The maximum adsorption capacity was 220.48 mg g^(-1).The FeCo@BDC adsorbents also own a prominent regeneration performance.The prominent performance of in the removal of trace Pb^(2+)makes FeCo@BDC an ideal candidate as commercial adsorbent materials.展开更多
Background:Acute pancreatitis(AP)is a severe disorder that leads to high morbidity and mortality.Appropriate reference genes are important for gene analysis in AP.This study sought to study the expression stability of...Background:Acute pancreatitis(AP)is a severe disorder that leads to high morbidity and mortality.Appropriate reference genes are important for gene analysis in AP.This study sought to study the expression stability of several reference genes in the golden Syrian hamster,a model of AP.Methods:AP was induced in golden Syrian hamster by intraperitoneal injection of ethanol(1.35 g/kg)and palmitoleic acid(2 mg/kg).The expression of candidate genes,including Actb,Gapdh,Eef2,Ywhaz,Rps18,Hprt1,Tubb,Rpl13a,Nono,and B2m,in hamster pancreas at different time points(1,3,6,9,and 24 h)posttreatment was analyzed using quantitative polymerase chain reaction.The expression stability of these genes was calculated using Best Keeper,Comprehensive Delta CT,Norm Finder,and ge Norm algorithms and Ref Finder software.Results:Our results show that the expression of these reference genes fluctuated during AP,of which Ywhaz and Gapdh were the most stable genes,whereas Tubb,Eef2,and Actb were the least stable genes.Furthermore,these genes were used to normalize the expression of TNF-αmessenger ribonucleic acid in inflamed pancreas.Conclusions:In conclusion,Ywhaz and Gapdh were suitable reference genes for gene expression analysis in AP induced in Syrian hamster.展开更多
BACKGROUND Previous studies have indicated bidirectional associations between urate levels and inflammatory bowel disease(IBD),including ulcerative colitis(UC)and Crohn’s disease(CD).However,it remains unclear whethe...BACKGROUND Previous studies have indicated bidirectional associations between urate levels and inflammatory bowel disease(IBD),including ulcerative colitis(UC)and Crohn’s disease(CD).However,it remains unclear whether the observations are causal because of confounding factors.AIM To investigate the causal associations between urate levels and IBD using bidirec-tional Mendelian randomization(MR).METHODS Independent genetic variants for urate levels and IBD were selected as instru-mental variables from published genome-wide association studies(GWASs).Summary statistics for instrument-outcome associations were retrieved from three separate databases for IBD(the UK Biobank,the FinnGen database and a large GWAS meta-analysis)and one for urate levels(a large GWAS meta-analysis).MR analyses included the inverse-variance-weighted method,weighted-median estimator,MR-Egger and sensitivity analyses(MR-PRESSO).A meta-analysis was also conducted to merge the data from separate outcome databases using a fixed-effects model.RESULTS Genetically higher serum urate levels were strongly associated with an increased risk of UC[odds ratio(OR):1.95,95%confidence interval(CI):1.86-2.05]after outlier correction,and the ORs(95%CIs)for IBD and CD were 0.94(95%CI:0.86-1.03)and 0.91(95%CI:0.80-1.04),respectively.Animal studies have confirmed the positive association between urate levels and UC.Moreover,genetically predicted IBD was inversely related to urate levels(OR:0.97,95%CI:0.94-0.99).However,no association was observed between genetically influenced UC or CD and urate levels.CONCLUSION Urate levels might be risk factors for UC,whereas genetically predicted IBD was inversely associated with urate levels.These findings provide essential new insight for treating and preventing IBD.展开更多
The areca palm(Areca catechu L.)is a vital tropical forest species widely utilized for vegetation restoration,landscaping,and greening purposes.Its nuts hold significant importance in traditional medicine,serving as r...The areca palm(Areca catechu L.)is a vital tropical forest species widely utilized for vegetation restoration,landscaping,and greening purposes.Its nuts hold significant importance in traditional medicine,serving as remedies for various ailments such as parasitic infections,digestive disorders,and depression[1–3].Areca palm yellow leaf disease(AYLD)poses a serious threat to areca palm growth.展开更多
The large-scale accumulation and pollution of solid mining waste is an urgent issue.Coal gangue is a prominent type of solid waste,and shows promise for use in high value-added products due to its content of many impo...The large-scale accumulation and pollution of solid mining waste is an urgent issue.Coal gangue is a prominent type of solid waste,and shows promise for use in high value-added products due to its content of many important compounds,including SiO_(2) and Al2O3.This study proposed the preparation of highly porous ceramics from coal gangue,coal slime,and coconut palm fibers.The ceramics were produced at a sintering temperature of 950℃ with a fiber content of 6 wt%,which led to the formation of porous ceramics with a porosity of 66.93%,volume density of 1.0329 g/cm^(3),compressive strength of 1.1025 MPa,and thermal conductivity is 0.3919 K(W/mk).A finite element model of the porous ceramics was established using the Abaqus module in ANSYS software,where the stress distribution and compressive strength were simulated.Further,the relationship between porosity and compressive strength was analyzed.The thermal properties of the porous ceramics were analyzed using the Fluent module,where the simulated changes in porosity under various sintering temperatures were consistent with the experimental data.The preparation of this highly porous ceramic from solid waste coal gangue shows promise for the minimizing the impact of waste gas and wastewater pollution in the future.展开更多
Capacity estimation plays a crucial role in battery management systems,and is essential for ensuring the safety and reliability of lithium-sulfur(Li-S)batteries.This paper proposes a method that uses a long short-term...Capacity estimation plays a crucial role in battery management systems,and is essential for ensuring the safety and reliability of lithium-sulfur(Li-S)batteries.This paper proposes a method that uses a long short-term memory(LSTM)neural network to estimate the state of health(SOH)of Li-S batteries.The method uses health features extracted from the charging curve and incre-mental capacity analysis(ICA)as input for the LSTM network.To enhance the robustness and accuracy of the network,the Adam algorithm is employed to optimize specific hyperparameters.Experimental data from three different groups of batteries with varying nominal capac-ities are used to validate the proposed method.The results demonstrate the effectiveness of the method in accurately estimating the capacity degradation of all three batteries.Also,the study examines the impact of different lengths of network training sets on capacity estimation.The results reveal that the ICA-LSTM model achieves a prediction accuracy of mean absolute error 4.6%and mean squared error 0.21%with three different training set lengths of 20%,40%,and 60%.The analysis demonstrates that the lightweight model maintains high SOH estimation accu-racy even with a small training set,and exhibits strong adaptive and generalization capabilities when applied to different Li-S batteries.Overall,the proposed method,supported by experimental validation and analysis,demonstrates its efficacy in ensuring accurate and reliable SOH estimation,thereby enhancing the safety and per-formance of Li-S batteries.Index Terms—Adam algorithm,incremental capacity analysis,Li-S battery,long short-term memory,state of health.展开更多
The multiple roles of the tumor necrosis factor(TNF)-α-inducible protein 8(TNFAIP8),also named TIPE family of proteins have been shown in tumor and inflammation progression and regulation of cellular autophagy and ap...The multiple roles of the tumor necrosis factor(TNF)-α-inducible protein 8(TNFAIP8),also named TIPE family of proteins have been shown in tumor and inflammation progression and regulation of cellular autophagy and apoptosis.In this review,we found that the TIPE family showed highly homologous sequences and conserved functional domains,such as the death effector domain(DED)-like domain but displayed different roles and mechanisms in different biological activities.For example,while TIPE is primarily associated with tumor progression and antitumor drug resistance,TIPE1 suppresses tumor progression in most instances.TIPE2 has multiple roles in tumor progression regulation,and antitumor drug resistance.Moreover,TIPE2 was also involved in inflammatory response regulation,tumor typing,and staging.A few studies reported that TIPE3 was engaged in tumor development by activating the phosphatidylinositol-3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.The structure,function,and mechanism of the TIPE family in cancer and inflammation have been summarized in this review.This might serve as a reference for further research on the TIPE family and shed new light on the crosstalk among antitumor responses,inflammation,and immunology.展开更多
The large accumulation of coal gangue,a common industrial solid waste,causes severe environmental problems,and green development strategies are required to transform this waste into high-value-added products.In this s...The large accumulation of coal gangue,a common industrial solid waste,causes severe environmental problems,and green development strategies are required to transform this waste into high-value-added products.In this study,low-cost ceramsites adsorbents were prepared from waste gangue,silt coal,and peanut shells and applied to remove the organic dye methylene blue from wastewater.We investigated the microstructure of ceramsites and the effects of the sintering atmosphere,sintering temperature,and solution pH on their adsorption performance.The ceramsites sintered at 800℃under a nitrogen atmosphere exhibited the largest three-dimensional-interconnected hierarchical porous structure among the prepared ceramsites;further,it exhibited the highest methylene blue adsorption performance,with an adsorption capacity of 0.954 mg·g^(−1),adsorption efficiency of over 95%,and adsorption equilibrium time of 1 h at a solution pH of 9.The removal efficiency remained greater than 75%after five adsorption cycles.The adsorption kinetics data were analyzed using various models,including the pseudosecond-order kinetic model and Langmuir equation,and the adsorption was attributed to electrostatic interactions between the dyes and ceramsites,n-interactions,and hydrogen bonds.The prepared coal gangue ceramsites exhibited excellent adsorption capacities,removal rates,and cyclic stabilities,demonstrating their promising application prospects for the comprehensive utilization of solid waste and for wastewater treatment.展开更多
BACKGROUND The molecular mechanisms of heart failure(HF) are still poorly understood. Circular RNA(circRNA) has been discovered in the heart in increasing numbers of studies. The goal of this research is to learn more...BACKGROUND The molecular mechanisms of heart failure(HF) are still poorly understood. Circular RNA(circRNA) has been discovered in the heart in increasing numbers of studies. The goal of this research is to learn more about the potential roles of circRNAs in HF.METHODS & RESULTS We used RNA sequencing data to identify the characteristics of circRNAs expressed in the heart and discovered that the majority of circRNAs screened were less than 2000 nt. Additionally, chromosomes One and Y had the most and least number of circRNAs, respectively. After excluding duplicate host genes and intergenic circRNAs, a total of 238 differentially expressed circRNAs(DECs) and 203 host genes were discovered. However, only four of the 203 host genes of DECs were examined in HF differentially expressed genes. Another study used Gene Oncology analysis of DECs host genes to elucidate the underlying pathogenesis of HF, and it found that binding and catalytic activity accounted for a large portion of DECs. Immune system, metabolism, and signal transduction pathways were significantly enriched. Furthermore, 1052 potentially regulated miRNAs from the top 40 DECs were collected to build a circRNA-mi RNA network, and it was discovered that 470 miRNAs can be regulated by multiple circRNAs, while others are regulated by a single circRNA. In addition, a comparison of the top 10m RNAs in HF and their targeted miRNAs revealed that DDX3Y and UTY were regulated by the most and least circRNA, respectively.CONCLUSION These findings demonstrated circRNAs have species and tissue specific expression patterns;while circRNA expression is independent on host genes, the same types of genes in DECs and DEGs worked in HF. Our findings would contribute to a better understanding of the critical roles of circRNAs and lay the groundwork for future studies of HF molecular functions.展开更多
Mosquitoes are an important medical insect and play a very important role in the process of biologically vector infectious diseases.A large number of bacterial communities are colonized in their intestines.In the proc...Mosquitoes are an important medical insect and play a very important role in the process of biologically vector infectious diseases.A large number of bacterial communities are colonized in their intestines.In the process of interaction and symbiosis with mosquitoes,the bacterial community is in many ways.The life activities of mosquitoes have a significant impact.This article reviews the population structure of the mosquito intestinal flora,influencing factors,and the regulation of mosquito vector efficacy by the flora.展开更多
More than 20 years ago,an old German man embarked on a quest to explore the suburbs of Shanghai and the villages of jiangsu Province in search of antique Chinese furmiture.He was not a mere antique collector or aficio...More than 20 years ago,an old German man embarked on a quest to explore the suburbs of Shanghai and the villages of jiangsu Province in search of antique Chinese furmiture.He was not a mere antique collector or aficionado of ancient furmiture,but a German scientist motivated by his profound love of Chinese culture.His name was Uli Schwarz(Fig.1),a distinguished German scientist who had dedicated the majority of his life to promoting cross-cultural scientific exchanges,an effort that left an indelible mark on the landscape of research collaborations between China and Germany.展开更多
Introduction:This study explores the midgut microbiota of mosquitoes in Haikou and Sanya cities,regions critical for understanding vector-borne disease dynamics in Hainan Province,China.It provides baseline data on mi...Introduction:This study explores the midgut microbiota of mosquitoes in Haikou and Sanya cities,regions critical for understanding vector-borne disease dynamics in Hainan Province,China.It provides baseline data on microbial composition and examines their potential role in influencing mosquito biology and vector competence,while highlighting the need for further research into their association with vectorborne viral infections.Methods:Adult mosquitoes were collected using light traps and human bait methods.Species identification was conducted through morphological examination and DNA barcoding using the cytochrome c oxidase subunit 1 gene(cox1).The V3–V4 hypervariable regions of the microbial 16S ribosomal RNA(rRNA)gene were sequenced using high-throughput methods to investigate the midgut microbiota.Statistical analyses,including Alpha and Beta diversity assessments of the sequencing results,were performed using SPSS 21.0 and R version 3.11.Results:The predominant mosquito species identified were Aedes albopictus,Armigeres subalbatus,and Culex pipiens.Microbiota analysis of 281 midguts revealed that Proteobacteria dominated(85.28%),with significant fractions being Alphaproteobacteria(52.14%),Gammaproteobacteria(29.90%),and Betaproteobacteria(3.22%).Other notable phyla included Firmicutes(6.24%),Actinobacteria(3.81%),and lesser quantities of Thermi,Cyanobacteria,and Bacteroidetes.Significant geographic variation in bacterial communities was observed between Haikou and Sanya(P<0.05),with unique taxa like Thermi and Cyanobacteria identified only in Haikou and Chlamydiae found solely in Sanya.The analysis revealed 204 overlapping species,with 473 unique to Haikou and 64 to Sanya.Conclusions:This study revealed significant geographic differences in the midgut microbiota of mosquitoes from Haikou and Sanya,providing foundational data for understanding their potential impact on mosquito biology and disease transmission.While the direct relationship between these microbial variations and vector-borne disease dynamics requires further investigation,these findings underscore the importance of mosquito microbiota research as part of broader strategies to mitigate vector-borne disease risks.展开更多
基金This study was financially supported by the National Natural Science Foundation of China(No.52172099)the Basic Research Plan of Natural Science of Shaanxi Province(No.2020JQ-754)+3 种基金the Key Innovation Team of Shaanxi Province(No.2014KCT-04)the Excellent Youth Science and Technology Fund Project of Xi'an University of Science and Technology(Grant No.6310221009)the Excellent Youth Science and Technology Fund Project of Xi'an University of Science and Technology(Grant No.6310221009)the Special Project of Shaanxi Province(No.19JK0490)and the Study on Preparation and Properties of New Solid-Wastebased Cementitious Materials(No.6000190120).
文摘Using coal gangue(CG)as raw material,a new type of all solid-waste-based 13-X molecular sieve material was controllably prepared by alkali fusion-hydrothermal method.The synthetic molecular sieve was used as a solid adsorbent to treat Cd^(2+)-containing wastewater,and its adsorption behavior on Cd^(2+)in aqueous solution was studied and analyzed.The microstructure and morphology of the molecular sieve were investigated by X-ray diffraction(XRD),field emission scanning electron microscopy(FESEM)and specific surface area analyzer.The results show that the synthesized 13-X molecular sieve has higher Brunauer–Emmett–Teller(BET)specific surface area with higher crystallinity and higher adsorption capacity for the heavy metal Cd^(2+).The adsorption process of Cd^(2+)by molecular sieve conforms to the Langmuir isotherm adsorption equation and Lagergren pseudo-second-order rate equation.Combined with thermodynamic calculation,it can be concluded that the adsorption process is physically monolayer,spontaneous and exothermic.In this study,a low-cost and naturally available synthesis method of 13-X molecular sieve is reported.Combined with its adsorption mechanism for Cd^(2+),it provides a feasible and general method for removing heavy metal ions from coal gangue and also provides a new way for the utilization of coal gangue with high added value.
基金financially supported by the Research Program of Application Foundation of Qinghai Province(No.2023-ZJ-744)the Natural Science Foundation(No.20212BAB204006)。
文摘Designing bifunctional oxygen reduction/evolution(ORR/OER)catalysts with high activity,robust stability and low cost is the key to accelerating the commercialization of rechargeable zinc-air battery(RZAB).Here,we propose a template-assisted electrospinning strategy to in situ fabricate 3D fibers consisting of FeNi nanoparticles embedded into N-doped hollow porous carbon nanospheres(FeNi@NHCFs)as the stable binder-free integrated air cathode in RZAB.3D interconnected conductive fiber networks provide fast electron transfer pathways and strengthen the mechanical flexibility.Meanwhile,N-doped hollow porous carbon nanospheres not only evenly confine FeNi nanoparticles to provide sufficient catalytic active sites,but also endow optimum mass transfer environment to reduce diffusion barrier.The RZABs assembled by FeNi@NHCFs as integrated air cathodes exhibit outstanding battery performance with high open-circuit voltage,large discharge specific capacity and power density,durable cyclic stability and great flexibility.Thus,this work brings a useful strategy to fabricate the integrated electrodes without using any polymeric binders for metal air batteries and other related fields.
基金Supported by Jinhua Municipal Science and Technology Bureau,No.2022-4-254.
文摘BACKGROUND Pancreatic cancer,characterized by aggressive proliferation and metastasis,is a lethal malignancy.The nightly hormone melatonin serves as a rhythm-regulating hormone,and is used to treat different cancers including pancreatic cancer.AIM To investigate how melatonin acts against human pancreatic cancer cell lines and analyze the biological processes that cause the observed effects.METHODS Panc-1 and AsPC-1 cells were treated with melatonin.Cell viability was measured using the cell counting kit-8 assay.Western blotting and immunofluorescence were used to analyze protein expression levels.Ferroptosis was measured by analyzing lipid reactive oxygen species and malondialdehyde levels;apoptosis was assessed using flow cytometry.RESULTS Melatonin significantly inhibited the viability,colony formation,migration,and invasion of Panc-1 and AsPC-1 cells.Additionally,melatonin activated the endoplasmic reticulum(ER)stress pathway(protein kinase R-like ER kinase eukaryotic initiation factor 2α-activating transcription factor 4),inhibited glutamine metabolism(alanine-serinecysteine transporter 2-glutaminase 1-glutathione peroxidase 4,alanine-serine-cysteine transporter 2-glutathione peroxidase 4),and promoted ferroptosis in pancreatic cancer cells.Co-treatment with a high melatonin concentration and protein kinase R-like ER kinase agonist(CCT020312)enhanced melatonin-induced ferroptosis in pancreatic cancer cells.Melatonin demonstrated a variety of anticancer effects by inhibiting autophagy.This was achieved through the increased expression of sequestosome-1 and decreased expression of light chain 3.Additionally,melatonin facilitated the promotion of apoptosis.CONCLUSION Melatonin induces ferroptosis in pancreatic cancer cells by activating transcription factor 4-dependent ER stress and inhibiting glutamine metabolism,promotes apoptosis in pancreatic cancer cells,and inhibits autophagy,leading to synergistic anticancer effects.
基金the financial support from the National Natural Science Foundation of China(22008032,22108034,and 22102026)the Guangdong Basic and Applied Basic Research Foundation(2019A1515110706)+2 种基金the Guangdong Provincial Key Lab of Green Chemical Product Technology(GC202111)the Medical Science and Technology Research Foundation of Guangdong Province(A2021189)the Shandong Provincial Natural Science Foundation(ZR2018ZC1458)。
文摘Nanoscale hierarchically porous metal–organic frameworks(NHP-MOFs)have received unprecedented attention in many fields owing to their integration of the strengths of nanoscale size(<1μm)and hierarchical porous structure(micro-,meso-and/or macro-pores)of MOFs.This review focuses on recent advances in the main synthetic strategies for NHP-MOFs based on different metal ions(e.g.,Cu,Fe,Co,Zn,Al,Zr,and Cr),including the template method,composite technology,post-synthetic modification,in situ growth and the grind method.In addition,the mechanisms of synthesis,regulation techniques and the advantages and disadvantages of various methods are discussed.Finally,the challenges and prospects of the commercialisation of promising NHP-MOFs are also presented.The purpose of this review is to provide a road map for future design and development of NHP-MOFs for practical application.
基金supported by the National Natural Science Foundation of China (Grant No.81373334)
文摘PEGylated liposomes are potential candidates to improve the pharmacokinetic characteristics of encapsulated drugs, to extend their circulation half-life and facilitate their passive accumulation at tumour sites. However, PEG-modified liposomes can induce accelerated blood clearance(ABC) upon repeated administration, and the extent of ABC phenomenon on the cytotoxic drugs-containing PEGylated liposomes is related to the dose of the cytotoxic drugs.In this study, EPI served as a model cytotoxic drug, a hydrophilic surfactant molecule,monosialylganglioside(GM1) was chosen and modified on the liposomes together with PEG.It was shown that upon mixed modification, when GM1 contents reached 10% or 15% mol,the ABC phenomenon of the PEGylated liposomal EPI significantly reduced. We also found that GM1 played an important role in abrogating the ABC phenomenon in both the induction phase and the effectuation phase. The results suggested that GM1 incorporation unfortunately did not avoid occurrence of ABC phenomenon completely, but GM1 modification on PEGylated liposomes may provide a significant improvement in clinical practice of PEGylated liposomes. Further study must be necessary.
文摘A novel Fe/Co metal organic complex nanosheet modified by 1,4-dicarboxybenzene(BDC),i.e.,FeCo@BDC,was prepared,and highly efficient removal performance for trace lead(Ⅱ)(Pb^(2+))was demonstrated in the neutral aqueous solutions.The removal rates were higher than 95%and the adsorption was equilibrated in 15 min.The isotherms and kinetics for the adsorption Pb^(2+)by the FeCo@BDC adsorbents followed Langmuir model and pseudo-second-order model,respectively.The maximum adsorption capacity was 220.48 mg g^(-1).The FeCo@BDC adsorbents also own a prominent regeneration performance.The prominent performance of in the removal of trace Pb^(2+)makes FeCo@BDC an ideal candidate as commercial adsorbent materials.
基金China Postdoctoral Science Foundation,Grant/Award Number:2021T140184Program for Science Technology Innovation Talents in Universities of Henan Province,Grant/Award Number:23HASTIT045Scientific Research of Traditional Chinese Medicine Specialized in Henan Province,Grant/Award Number:2022ZY1172。
文摘Background:Acute pancreatitis(AP)is a severe disorder that leads to high morbidity and mortality.Appropriate reference genes are important for gene analysis in AP.This study sought to study the expression stability of several reference genes in the golden Syrian hamster,a model of AP.Methods:AP was induced in golden Syrian hamster by intraperitoneal injection of ethanol(1.35 g/kg)and palmitoleic acid(2 mg/kg).The expression of candidate genes,including Actb,Gapdh,Eef2,Ywhaz,Rps18,Hprt1,Tubb,Rpl13a,Nono,and B2m,in hamster pancreas at different time points(1,3,6,9,and 24 h)posttreatment was analyzed using quantitative polymerase chain reaction.The expression stability of these genes was calculated using Best Keeper,Comprehensive Delta CT,Norm Finder,and ge Norm algorithms and Ref Finder software.Results:Our results show that the expression of these reference genes fluctuated during AP,of which Ywhaz and Gapdh were the most stable genes,whereas Tubb,Eef2,and Actb were the least stable genes.Furthermore,these genes were used to normalize the expression of TNF-αmessenger ribonucleic acid in inflamed pancreas.Conclusions:In conclusion,Ywhaz and Gapdh were suitable reference genes for gene expression analysis in AP induced in Syrian hamster.
基金Supported by National Natural Science Foundation of China,No.82170567,No.81873546,No.82170568,and No.82300627Program of Shanghai Academic/Technology Research Leader,No.22XD1425000+4 种基金The"Shu Guang"project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation,No.19SG30,ChinaDeep Blue Project of Naval Medical University(Pilot Talent Plan)The Chenguang Program of Shanghai Education Development Foundation and Shanghai Municipal Education Commission,No.22CGA42The Shanghai Sailing Program,No.23YF1458600and Shanghai Natural Science Foundation,No.23ZR1478700.
文摘BACKGROUND Previous studies have indicated bidirectional associations between urate levels and inflammatory bowel disease(IBD),including ulcerative colitis(UC)and Crohn’s disease(CD).However,it remains unclear whether the observations are causal because of confounding factors.AIM To investigate the causal associations between urate levels and IBD using bidirec-tional Mendelian randomization(MR).METHODS Independent genetic variants for urate levels and IBD were selected as instru-mental variables from published genome-wide association studies(GWASs).Summary statistics for instrument-outcome associations were retrieved from three separate databases for IBD(the UK Biobank,the FinnGen database and a large GWAS meta-analysis)and one for urate levels(a large GWAS meta-analysis).MR analyses included the inverse-variance-weighted method,weighted-median estimator,MR-Egger and sensitivity analyses(MR-PRESSO).A meta-analysis was also conducted to merge the data from separate outcome databases using a fixed-effects model.RESULTS Genetically higher serum urate levels were strongly associated with an increased risk of UC[odds ratio(OR):1.95,95%confidence interval(CI):1.86-2.05]after outlier correction,and the ORs(95%CIs)for IBD and CD were 0.94(95%CI:0.86-1.03)and 0.91(95%CI:0.80-1.04),respectively.Animal studies have confirmed the positive association between urate levels and UC.Moreover,genetically predicted IBD was inversely related to urate levels(OR:0.97,95%CI:0.94-0.99).However,no association was observed between genetically influenced UC or CD and urate levels.CONCLUSION Urate levels might be risk factors for UC,whereas genetically predicted IBD was inversely associated with urate levels.These findings provide essential new insight for treating and preventing IBD.
基金The raw sequences of AYLP(short reads S1:SRR19151409,short reads S2:SRR29507615,long reads S1:SRR29507613,long reads S2:SRR29507612)have been deposited into NCBI Sequence Read Archive under the BioProject PRJNA835721The draft genome sequence has been deposited into the NCBI genome database(CP169277).
文摘The areca palm(Areca catechu L.)is a vital tropical forest species widely utilized for vegetation restoration,landscaping,and greening purposes.Its nuts hold significant importance in traditional medicine,serving as remedies for various ailments such as parasitic infections,digestive disorders,and depression[1–3].Areca palm yellow leaf disease(AYLD)poses a serious threat to areca palm growth.
基金National Natural Science Foundation of China under Grant No.(51372197)Key Innovation Team of Shaanxi Province under Grant No.(2014KCT-04)Provincial Joint Fund of Shaanxi(2021JLM-28).
文摘The large-scale accumulation and pollution of solid mining waste is an urgent issue.Coal gangue is a prominent type of solid waste,and shows promise for use in high value-added products due to its content of many important compounds,including SiO_(2) and Al2O3.This study proposed the preparation of highly porous ceramics from coal gangue,coal slime,and coconut palm fibers.The ceramics were produced at a sintering temperature of 950℃ with a fiber content of 6 wt%,which led to the formation of porous ceramics with a porosity of 66.93%,volume density of 1.0329 g/cm^(3),compressive strength of 1.1025 MPa,and thermal conductivity is 0.3919 K(W/mk).A finite element model of the porous ceramics was established using the Abaqus module in ANSYS software,where the stress distribution and compressive strength were simulated.Further,the relationship between porosity and compressive strength was analyzed.The thermal properties of the porous ceramics were analyzed using the Fluent module,where the simulated changes in porosity under various sintering temperatures were consistent with the experimental data.The preparation of this highly porous ceramic from solid waste coal gangue shows promise for the minimizing the impact of waste gas and wastewater pollution in the future.
基金This work is supported by the Zhejiang Province Natural Science Foundation(No.LY22E070007)National Natural Science Foundation of China(No.52007170).
文摘Capacity estimation plays a crucial role in battery management systems,and is essential for ensuring the safety and reliability of lithium-sulfur(Li-S)batteries.This paper proposes a method that uses a long short-term memory(LSTM)neural network to estimate the state of health(SOH)of Li-S batteries.The method uses health features extracted from the charging curve and incre-mental capacity analysis(ICA)as input for the LSTM network.To enhance the robustness and accuracy of the network,the Adam algorithm is employed to optimize specific hyperparameters.Experimental data from three different groups of batteries with varying nominal capac-ities are used to validate the proposed method.The results demonstrate the effectiveness of the method in accurately estimating the capacity degradation of all three batteries.Also,the study examines the impact of different lengths of network training sets on capacity estimation.The results reveal that the ICA-LSTM model achieves a prediction accuracy of mean absolute error 4.6%and mean squared error 0.21%with three different training set lengths of 20%,40%,and 60%.The analysis demonstrates that the lightweight model maintains high SOH estimation accu-racy even with a small training set,and exhibits strong adaptive and generalization capabilities when applied to different Li-S batteries.Overall,the proposed method,supported by experimental validation and analysis,demonstrates its efficacy in ensuring accurate and reliable SOH estimation,thereby enhancing the safety and per-formance of Li-S batteries.Index Terms—Adam algorithm,incremental capacity analysis,Li-S battery,long short-term memory,state of health.
基金supported by the Medical Scientific Research Foundation of Guangdong Province of China(No.A2021236)2022 Guangdong Provincial Education Science Planning Project(Higher Education Special Project,No.2022GXJK221)+4 种基金the 2021 Open Project Fund of Guangdong Provincial Key Laboratory of Medicinal Functional Gene Research,the National Key Clinical Specialty Construction Project(Clinical Pharmacy)and High-Level Clinical Key Specialty(Clinical Pharmacy)in Guangdong Province,Science and Technology Program of Guangzhou,China(No.202201010154)the Special Fund for the Cultivation of Scientific and Technological Innovation of College Students in Guangdong Province of China(No.pdjh2022b0270)the College Students’Innovation and Entrepreneurship Training Project of Guangdong Province(Nos.202210573054,202210573041)Special Fund for the Cultivation of National Natural Science Foundation of China in School of Clinical Pharmacy,Guangdong Pharmaceutical University(No.SCP2022-03)Jinghua(Zhejiang Province)Science and Technology Research Program Project(No.2021-4-135).
文摘The multiple roles of the tumor necrosis factor(TNF)-α-inducible protein 8(TNFAIP8),also named TIPE family of proteins have been shown in tumor and inflammation progression and regulation of cellular autophagy and apoptosis.In this review,we found that the TIPE family showed highly homologous sequences and conserved functional domains,such as the death effector domain(DED)-like domain but displayed different roles and mechanisms in different biological activities.For example,while TIPE is primarily associated with tumor progression and antitumor drug resistance,TIPE1 suppresses tumor progression in most instances.TIPE2 has multiple roles in tumor progression regulation,and antitumor drug resistance.Moreover,TIPE2 was also involved in inflammatory response regulation,tumor typing,and staging.A few studies reported that TIPE3 was engaged in tumor development by activating the phosphatidylinositol-3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.The structure,function,and mechanism of the TIPE family in cancer and inflammation have been summarized in this review.This might serve as a reference for further research on the TIPE family and shed new light on the crosstalk among antitumor responses,inflammation,and immunology.
基金supported by the Natural Science Foundation of China under Grant(No.52172099)the Provincial Joint Fund of Shaanxi(2021JLM-28).
文摘The large accumulation of coal gangue,a common industrial solid waste,causes severe environmental problems,and green development strategies are required to transform this waste into high-value-added products.In this study,low-cost ceramsites adsorbents were prepared from waste gangue,silt coal,and peanut shells and applied to remove the organic dye methylene blue from wastewater.We investigated the microstructure of ceramsites and the effects of the sintering atmosphere,sintering temperature,and solution pH on their adsorption performance.The ceramsites sintered at 800℃under a nitrogen atmosphere exhibited the largest three-dimensional-interconnected hierarchical porous structure among the prepared ceramsites;further,it exhibited the highest methylene blue adsorption performance,with an adsorption capacity of 0.954 mg·g^(−1),adsorption efficiency of over 95%,and adsorption equilibrium time of 1 h at a solution pH of 9.The removal efficiency remained greater than 75%after five adsorption cycles.The adsorption kinetics data were analyzed using various models,including the pseudosecond-order kinetic model and Langmuir equation,and the adsorption was attributed to electrostatic interactions between the dyes and ceramsites,n-interactions,and hydrogen bonds.The prepared coal gangue ceramsites exhibited excellent adsorption capacities,removal rates,and cyclic stabilities,demonstrating their promising application prospects for the comprehensive utilization of solid waste and for wastewater treatment.
基金funded by the National Natural Science Foundation of China(81900245,81730009,81941002 and 81700256).
文摘BACKGROUND The molecular mechanisms of heart failure(HF) are still poorly understood. Circular RNA(circRNA) has been discovered in the heart in increasing numbers of studies. The goal of this research is to learn more about the potential roles of circRNAs in HF.METHODS & RESULTS We used RNA sequencing data to identify the characteristics of circRNAs expressed in the heart and discovered that the majority of circRNAs screened were less than 2000 nt. Additionally, chromosomes One and Y had the most and least number of circRNAs, respectively. After excluding duplicate host genes and intergenic circRNAs, a total of 238 differentially expressed circRNAs(DECs) and 203 host genes were discovered. However, only four of the 203 host genes of DECs were examined in HF differentially expressed genes. Another study used Gene Oncology analysis of DECs host genes to elucidate the underlying pathogenesis of HF, and it found that binding and catalytic activity accounted for a large portion of DECs. Immune system, metabolism, and signal transduction pathways were significantly enriched. Furthermore, 1052 potentially regulated miRNAs from the top 40 DECs were collected to build a circRNA-mi RNA network, and it was discovered that 470 miRNAs can be regulated by multiple circRNAs, while others are regulated by a single circRNA. In addition, a comparison of the top 10m RNAs in HF and their targeted miRNAs revealed that DDX3Y and UTY were regulated by the most and least circRNA, respectively.CONCLUSION These findings demonstrated circRNAs have species and tissue specific expression patterns;while circRNA expression is independent on host genes, the same types of genes in DECs and DEGs worked in HF. Our findings would contribute to a better understanding of the critical roles of circRNAs and lay the groundwork for future studies of HF molecular functions.
基金National Science and Technology Major Project of China(No.2018Z X10101003-001-009)。
文摘Mosquitoes are an important medical insect and play a very important role in the process of biologically vector infectious diseases.A large number of bacterial communities are colonized in their intestines.In the process of interaction and symbiosis with mosquitoes,the bacterial community is in many ways.The life activities of mosquitoes have a significant impact.This article reviews the population structure of the mosquito intestinal flora,influencing factors,and the regulation of mosquito vector efficacy by the flora.
文摘More than 20 years ago,an old German man embarked on a quest to explore the suburbs of Shanghai and the villages of jiangsu Province in search of antique Chinese furmiture.He was not a mere antique collector or aficionado of ancient furmiture,but a German scientist motivated by his profound love of Chinese culture.His name was Uli Schwarz(Fig.1),a distinguished German scientist who had dedicated the majority of his life to promoting cross-cultural scientific exchanges,an effort that left an indelible mark on the landscape of research collaborations between China and Germany.
基金Supported by the Hainan Provincial Natural Science Foundation of China(824QN269,822QN324)Hainan Province Science and Technology Talent Innovation Project(KJRC2023D29)+1 种基金Hainan Tropical Disease Research Center(Hainan Sub-Center,Chinese Center for Tropical Disease Research)(HNTDC202303)National Key Plan for Scientific Research and Development of China(2023YFA1801002).
文摘Introduction:This study explores the midgut microbiota of mosquitoes in Haikou and Sanya cities,regions critical for understanding vector-borne disease dynamics in Hainan Province,China.It provides baseline data on microbial composition and examines their potential role in influencing mosquito biology and vector competence,while highlighting the need for further research into their association with vectorborne viral infections.Methods:Adult mosquitoes were collected using light traps and human bait methods.Species identification was conducted through morphological examination and DNA barcoding using the cytochrome c oxidase subunit 1 gene(cox1).The V3–V4 hypervariable regions of the microbial 16S ribosomal RNA(rRNA)gene were sequenced using high-throughput methods to investigate the midgut microbiota.Statistical analyses,including Alpha and Beta diversity assessments of the sequencing results,were performed using SPSS 21.0 and R version 3.11.Results:The predominant mosquito species identified were Aedes albopictus,Armigeres subalbatus,and Culex pipiens.Microbiota analysis of 281 midguts revealed that Proteobacteria dominated(85.28%),with significant fractions being Alphaproteobacteria(52.14%),Gammaproteobacteria(29.90%),and Betaproteobacteria(3.22%).Other notable phyla included Firmicutes(6.24%),Actinobacteria(3.81%),and lesser quantities of Thermi,Cyanobacteria,and Bacteroidetes.Significant geographic variation in bacterial communities was observed between Haikou and Sanya(P<0.05),with unique taxa like Thermi and Cyanobacteria identified only in Haikou and Chlamydiae found solely in Sanya.The analysis revealed 204 overlapping species,with 473 unique to Haikou and 64 to Sanya.Conclusions:This study revealed significant geographic differences in the midgut microbiota of mosquitoes from Haikou and Sanya,providing foundational data for understanding their potential impact on mosquito biology and disease transmission.While the direct relationship between these microbial variations and vector-borne disease dynamics requires further investigation,these findings underscore the importance of mosquito microbiota research as part of broader strategies to mitigate vector-borne disease risks.
