An endophytic fungus Pestalotiopsis microspora isolated from the fruits of Manilkara zapota was cultured in potato dextrose broth media.Chromatographic separation of the EtOAc extract of the broth and mycelium led to ...An endophytic fungus Pestalotiopsis microspora isolated from the fruits of Manilkara zapota was cultured in potato dextrose broth media.Chromatographic separation of the EtOAc extract of the broth and mycelium led to the isolation of a new azaphilonoid named pitholide E(1),in addition to previously identified pitholide B(2),pitholide D(3),pestalotin(LL-P880α)(4),PC-2(5),LL-P880β(6),tyrosol(7)and 4-oxo-4H-pyran-3-acetic acid(8).An endophytic fungus P.microspora from M.zapota and the isolation of compounds 1-5,7 and 8 from P.microspora are reported here for the first time.展开更多
Neofusicoccum parvum,an endophytic fungus isolated from Elaeocarpus serratus(Ceylon Olive;family Elaeocarpaceae),was grown for 3 weeks in potato dextrose broth.Chromatographic separation of the ethyl acetate extracts ...Neofusicoccum parvum,an endophytic fungus isolated from Elaeocarpus serratus(Ceylon Olive;family Elaeocarpaceae),was grown for 3 weeks in potato dextrose broth.Chromatographic separation of the ethyl acetate extracts from the culture filtrate and mycelium over silica gel,Sephadex LH-20 and preparative thin layer chromatography furnished(R)-7-hydroxymellein(1),(3R,4R)-4-hydroxymellein(2),(3R,4S)-4-hydroxymellein(3),(R)-5-hydroxymellein(4),(R)-mellein(5),(3R,4R)-4,7-dihydroxymellein(6),(6R,7S)-dia-asperlin(7),CJ-14445(8)and 13,14,15,16-tetranorlabd-7-ene-19,6β:12,17-diolide(9).The structures of known compounds 1–9 were determined by spectroscopic methods and comparison with reported data.This is the first report of the isolation of an endophytic fungus from E.serratus,and the isolation of compounds 1,4,6,8 and 9 from N.parvum.It is important to note that compounds 1–7 are small molecules with an oxygen heterocyclic ring system.These compounds can be used as starting materials in the synthesis of pharmaceutically important large molecules with oxygen heterocyles.展开更多
An endophytic fungus Aspergillus fumigatus,isolated from the fruits of Solanum insanum L.,was cultured in potato dextrose broth medium.Chromatographic separation of the EtOAc extract led to the isolation of six second...An endophytic fungus Aspergillus fumigatus,isolated from the fruits of Solanum insanum L.,was cultured in potato dextrose broth medium.Chromatographic separation of the EtOAc extract led to the isolation of six secondary metabolites,identified as pseurotin A(1),fumigaclavine C(2),monomethylsulochrin(3),trypacidin(4),fumiquinazoline C(5)and guignasulfide(6)by spectral means.Compounds 2 and 3 showed brine shrimp toxicity with IC50 values of 147 and 74.2μM,respectively,while compounds 4 and 5 showed slight inhibition on the growth of fungal pathogen Cladosporium cladosporioides.This is the first report of the isolation of guignasulfide(6)from A.fumigatus,which has been previously isolated only from an endophytic fungus Guignardia sp.展开更多
基金Financial support from the National Research Council(Research Grant NRC 12–032)is gratefully acknowledged.
文摘An endophytic fungus Pestalotiopsis microspora isolated from the fruits of Manilkara zapota was cultured in potato dextrose broth media.Chromatographic separation of the EtOAc extract of the broth and mycelium led to the isolation of a new azaphilonoid named pitholide E(1),in addition to previously identified pitholide B(2),pitholide D(3),pestalotin(LL-P880α)(4),PC-2(5),LL-P880β(6),tyrosol(7)and 4-oxo-4H-pyran-3-acetic acid(8).An endophytic fungus P.microspora from M.zapota and the isolation of compounds 1-5,7 and 8 from P.microspora are reported here for the first time.
文摘Neofusicoccum parvum,an endophytic fungus isolated from Elaeocarpus serratus(Ceylon Olive;family Elaeocarpaceae),was grown for 3 weeks in potato dextrose broth.Chromatographic separation of the ethyl acetate extracts from the culture filtrate and mycelium over silica gel,Sephadex LH-20 and preparative thin layer chromatography furnished(R)-7-hydroxymellein(1),(3R,4R)-4-hydroxymellein(2),(3R,4S)-4-hydroxymellein(3),(R)-5-hydroxymellein(4),(R)-mellein(5),(3R,4R)-4,7-dihydroxymellein(6),(6R,7S)-dia-asperlin(7),CJ-14445(8)and 13,14,15,16-tetranorlabd-7-ene-19,6β:12,17-diolide(9).The structures of known compounds 1–9 were determined by spectroscopic methods and comparison with reported data.This is the first report of the isolation of an endophytic fungus from E.serratus,and the isolation of compounds 1,4,6,8 and 9 from N.parvum.It is important to note that compounds 1–7 are small molecules with an oxygen heterocyclic ring system.These compounds can be used as starting materials in the synthesis of pharmaceutically important large molecules with oxygen heterocyles.
基金Financial assistance from the National Science Foundation of Sri Lanka(Grant No RG/2014/BS/02)is gratefully acknowledged.
文摘An endophytic fungus Aspergillus fumigatus,isolated from the fruits of Solanum insanum L.,was cultured in potato dextrose broth medium.Chromatographic separation of the EtOAc extract led to the isolation of six secondary metabolites,identified as pseurotin A(1),fumigaclavine C(2),monomethylsulochrin(3),trypacidin(4),fumiquinazoline C(5)and guignasulfide(6)by spectral means.Compounds 2 and 3 showed brine shrimp toxicity with IC50 values of 147 and 74.2μM,respectively,while compounds 4 and 5 showed slight inhibition on the growth of fungal pathogen Cladosporium cladosporioides.This is the first report of the isolation of guignasulfide(6)from A.fumigatus,which has been previously isolated only from an endophytic fungus Guignardia sp.
