An eco-friendly and fast HPLC method was developed for the determination of adenosine, inosine, guanosine and uridine in Cordyceps and related products(fermented mycelia of Hirsutella sinensis and Paecilomyces hepiali...An eco-friendly and fast HPLC method was developed for the determination of adenosine, inosine, guanosine and uridine in Cordyceps and related products(fermented mycelia of Hirsutella sinensis and Paecilomyces hepiali). The sample was ultrasonically extracted using 0.5% phosphoric acid solutions for 2.5 min. Sample separation was performed on a Poroshell SB-Aq column(50 mm × 4.6 mm, 2.7 μm) using eco-friendly mobile phase consisting of formic acid and ammonium formate aqueous solution at a flow rate of 1.0 m L·min;. The detection wavelength was 260 nm. The developed HPLC method showed good linearity with correlation coefficients of 1.0000 in the test range. Good precision, repeatability and stability of this method were also observed(RSD ≤ 2.81%).The recovery ranged from 91.84%-105.19%(RSD ≤ 2.59%). Compared with reported methods, the current method did not use harmful organic solvent and took only 10.5 min. It obtained a high eco-score of 91 by the "Analytical Eco-Scale" tool. The developed method is eco-friendly and fast, which is suitable for the quality evaluation of Cordyceps and related products.展开更多
基金supported by the National Natural Science Foundation of China (Nos.81925037, 82073976, and 31700013)the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (No.2017BT01Y036)+2 种基金Guangdong Natural Science Funds for Distinguished Young Scholar(No.2021B1515020065)the Young Elite Scientists Sponsorship Program by the China Association of Chinese Medicine (No.QNRC2-B06)the Dongguan Academician Workstation Project (No.DGYSZ-2018-03)。
文摘An eco-friendly and fast HPLC method was developed for the determination of adenosine, inosine, guanosine and uridine in Cordyceps and related products(fermented mycelia of Hirsutella sinensis and Paecilomyces hepiali). The sample was ultrasonically extracted using 0.5% phosphoric acid solutions for 2.5 min. Sample separation was performed on a Poroshell SB-Aq column(50 mm × 4.6 mm, 2.7 μm) using eco-friendly mobile phase consisting of formic acid and ammonium formate aqueous solution at a flow rate of 1.0 m L·min;. The detection wavelength was 260 nm. The developed HPLC method showed good linearity with correlation coefficients of 1.0000 in the test range. Good precision, repeatability and stability of this method were also observed(RSD ≤ 2.81%).The recovery ranged from 91.84%-105.19%(RSD ≤ 2.59%). Compared with reported methods, the current method did not use harmful organic solvent and took only 10.5 min. It obtained a high eco-score of 91 by the "Analytical Eco-Scale" tool. The developed method is eco-friendly and fast, which is suitable for the quality evaluation of Cordyceps and related products.
