以荷叶为原料,以去离子水为溶剂提取荷叶多糖(Polysaccharide from Nelumbinis Folium NFP),经过DEAE-Sepharose Fast Flow阴离子交换柱和Sephadex G-100凝胶色谱柱纯化得到荷叶多糖NFP-0.8-b.在此基础上,优化确定NFP-0.8-b的最佳磷酸...以荷叶为原料,以去离子水为溶剂提取荷叶多糖(Polysaccharide from Nelumbinis Folium NFP),经过DEAE-Sepharose Fast Flow阴离子交换柱和Sephadex G-100凝胶色谱柱纯化得到荷叶多糖NFP-0.8-b.在此基础上,优化确定NFP-0.8-b的最佳磷酸化修饰工艺,并探讨磷酸化NFP-0.8-b(Phosphorylated NFP-0.8-b,Ph-NFP-0.8-b)的抗氧化活性及其在曲奇生产过程中对晚期糖基化末端产物的抑制作用.结果表明:NFP-0.8-b磷酸化最佳工艺为:磷酸化试剂比例(三聚磷酸钠︰三偏磷酸钠)为5︰2,反应温度75 ℃,pH 9.0,反应时间3.5 h,在此条件下磷酸根含量达8.39%.与NFP-0.8-b相比,Ph-NFP-0.8-b对DPPH自由基和羟基自由基的清除作用进一步增强.多糖添加量大于5%时,Ph-NFP-0.8-b对曲奇生产过程中晚期糖基化末端产物的抑制作用明显强于NFP-0.8-b(P<0.05),揭示Ph-NFP-0.8-b可以作为抗氧化剂及晚期糖基化末端产物抑制剂应用于曲奇的工业化生产.展开更多
Objective: to apply and evaluate the practical application value of xpertMTB/RIF detection technology in the diagnosis of related diseases and follow-up treatment, and to evaluate the reliability of the diagnosis of t...Objective: to apply and evaluate the practical application value of xpertMTB/RIF detection technology in the diagnosis of related diseases and follow-up treatment, and to evaluate the reliability of the diagnosis of tuberculosis and the safety of the follow-up use of rifampicin in primary units. Methods: xpertMTB/RIF test was performed on smear-positive tuberculosis patients in Qionghai city, Hainan Province from 2015 to 2019. Sputum culture was performed on rifampicin-resistant patients and negative patients at the same time, and the sputum was sent to the provincial reference laboratory for drug sensitivity test and strain identification. From 2015 to 2019, sputum and other body fluids samples of suspected tuberculosis patients at the outpatient clinic of Qionghai City, Hainan province. Results: according to the traditional drug sensitivity test and strain identification as the gold standard, a total of 48 tuberculosis patients were detected from 60 smear-negative sputum specimens of children and 66 other body fluids of children and adults. XpertMTB /RIF detected drug resistance and negative sputum samples in 65 samples. Through strain identification, the traditional drug sensitivity test showed rifampicin resistance in 43 cases, multidrug resistance in 4 cases, and non-tuberculosis mycobacterium (NTM) in 16 cases. XpertMTB /RIF has higher sensitivity to mycobacterium tuberculosis than smear microscopy and Rifampicin resistance to xpertMTB/RIF. Conclusion: xpertMTB/RIF test just need a simple operation can be very precise inspection data as a result, further analysis can even get to rifampin resistance in patients with specific conditions, for detection of mycobacterium tuberculosis (MTB) is the sensitivity of the ideal, can be used for tuberculosis at the grass-roots level in our country fixed-point hospital.展开更多
文摘Objective: to apply and evaluate the practical application value of xpertMTB/RIF detection technology in the diagnosis of related diseases and follow-up treatment, and to evaluate the reliability of the diagnosis of tuberculosis and the safety of the follow-up use of rifampicin in primary units. Methods: xpertMTB/RIF test was performed on smear-positive tuberculosis patients in Qionghai city, Hainan Province from 2015 to 2019. Sputum culture was performed on rifampicin-resistant patients and negative patients at the same time, and the sputum was sent to the provincial reference laboratory for drug sensitivity test and strain identification. From 2015 to 2019, sputum and other body fluids samples of suspected tuberculosis patients at the outpatient clinic of Qionghai City, Hainan province. Results: according to the traditional drug sensitivity test and strain identification as the gold standard, a total of 48 tuberculosis patients were detected from 60 smear-negative sputum specimens of children and 66 other body fluids of children and adults. XpertMTB /RIF detected drug resistance and negative sputum samples in 65 samples. Through strain identification, the traditional drug sensitivity test showed rifampicin resistance in 43 cases, multidrug resistance in 4 cases, and non-tuberculosis mycobacterium (NTM) in 16 cases. XpertMTB /RIF has higher sensitivity to mycobacterium tuberculosis than smear microscopy and Rifampicin resistance to xpertMTB/RIF. Conclusion: xpertMTB/RIF test just need a simple operation can be very precise inspection data as a result, further analysis can even get to rifampin resistance in patients with specific conditions, for detection of mycobacterium tuberculosis (MTB) is the sensitivity of the ideal, can be used for tuberculosis at the grass-roots level in our country fixed-point hospital.
