The response to endoplasmic reticulum(ER)stress has been noted in both human depression cases and depression models in rodents.Wuling powder,derived from the mycelium of the esteemed fungus Xylaria Nigripes(Kl.)Sacc,h...The response to endoplasmic reticulum(ER)stress has been noted in both human depression cases and depression models in rodents.Wuling powder,derived from the mycelium of the esteemed fungus Xylaria Nigripes(Kl.)Sacc,has demonstrated efficacy in alleviating depressive symptoms.The purpose of this research was to explore the antidepressant properties of Wuling powder and its basic molecular effects,particularly regarding alterations in ER stress.A model of social defeat stress was created by introducing a mouse to the cage of an unfamiliar,hostile mouse for intervals of 5–10 min daily over a span of 10 d.Subsequently,the mice received oral doses of Wuling powder for 2 weeks.The social approach-avoidance assay was employed to evaluate signs of depression-like behaviors.Moreover,protein and gene expressions linked to ER stress triggered by social defeat were analyzed through Western blotting analysis and quantitative real-time PCR.The behavioral tests indicated that Wuling powder ameliorated behaviors associated with depression due to social defeat stress.Treatment with Wuling powder markedly reduced the increased levels of the 78-k Da glucose-regulated protein and protein disulfide isomerase caused by social defeat stress.It also diminished the expression of inositol-requiring enzyme 1α(IRE1α)and spliced X box-binding protein-1(s XBP1)at the protein and m RNA levels.Furthermore,Wuling treatment notably decreased the levels of phosphorylated eukaryotic initiation factor 2 alpha kinase(P-e IF2α),activating transcription factor 4(ATF4)and C/EBP homologous protein(CHOP),simultaneously enhancing the ratio of B-cell lymphoma 2(Bcl-2)to Bcl-2-associated X protein(Bax).These results suggested that Wuling powder could alleviate ER stress and inhibit cell apoptosis in the hippocampus by inhibiting protein translation and synthesis,thereby attenuating depressive-like behavior.展开更多
清华大学哲学系卢风教授,系苏格兰圣安德鲁斯大学(1999年)、韩国延世大学(2004—2005年)和美国哈佛-燕京学社(2007年)访问学者,曾任清华大学哲学系主任、北京市政协委员、中国自然辩证法研究会环境哲学专业委员会主任等职,以及国内期刊...清华大学哲学系卢风教授,系苏格兰圣安德鲁斯大学(1999年)、韩国延世大学(2004—2005年)和美国哈佛-燕京学社(2007年)访问学者,曾任清华大学哲学系主任、北京市政协委员、中国自然辩证法研究会环境哲学专业委员会主任等职,以及国内期刊《自然辩证法通讯》、《科学技术与辩证法》(后改名为《科学技术哲学研究》)和国际期刊《环境伦理学》(Environmental Ethics)、韩国期刊International Journal of Contextual Theology in East Asia等编委,现为清华大学生态文明研究中心研究员。他是国内最早关注生态文明的学者之一,多年来致力于环境伦理学、技术伦理学和生态哲学等领域的研究,曾主持完成国家社会科学基金重点项目“生态文明建设中的伦理问题研究”、一般项目“西方近现代价值观批判”以及多项省部级课题,出版学术专著《人类的家园——现代文化矛盾的哲学反思》(1996年)、《享乐与生存——现代人的生活方式与环境保护》(2000年)、《启蒙之后——近现代西方人价值追求的得与失》(2003年)、《应用伦理学与当代生活》(2004年)、《从现代文明到生态文明》(2009年)、《现代性与物欲的释放:杜维明先生访谈录》(2009年)、《人、环境与自然——环境哲学导论》(2011年)、《科技、自由与自然——科技伦理与环境伦理前沿问题研究》(2011年)、《非物质经济、文化与生态文明》(2015年)、《生态文明与美丽中国》(2019年)和《生态文明与生态哲学》(与王远哲合著,2022年)等多部,并公开发表学术论文300余篇。近期,《鄱阳湖学刊》编辑部王俊暐就生态文明的理论基石、研究方法和践行思路等问题与卢风教授展开了深入交流,现将访谈内容辑录如下,以飨读者。展开更多
基金National Natural Science Foundation of China(Grant No.81100801 and 81050025)the foundation of Zhejiang Jolly Pharmaceutical Co.,Ltd.
文摘The response to endoplasmic reticulum(ER)stress has been noted in both human depression cases and depression models in rodents.Wuling powder,derived from the mycelium of the esteemed fungus Xylaria Nigripes(Kl.)Sacc,has demonstrated efficacy in alleviating depressive symptoms.The purpose of this research was to explore the antidepressant properties of Wuling powder and its basic molecular effects,particularly regarding alterations in ER stress.A model of social defeat stress was created by introducing a mouse to the cage of an unfamiliar,hostile mouse for intervals of 5–10 min daily over a span of 10 d.Subsequently,the mice received oral doses of Wuling powder for 2 weeks.The social approach-avoidance assay was employed to evaluate signs of depression-like behaviors.Moreover,protein and gene expressions linked to ER stress triggered by social defeat were analyzed through Western blotting analysis and quantitative real-time PCR.The behavioral tests indicated that Wuling powder ameliorated behaviors associated with depression due to social defeat stress.Treatment with Wuling powder markedly reduced the increased levels of the 78-k Da glucose-regulated protein and protein disulfide isomerase caused by social defeat stress.It also diminished the expression of inositol-requiring enzyme 1α(IRE1α)and spliced X box-binding protein-1(s XBP1)at the protein and m RNA levels.Furthermore,Wuling treatment notably decreased the levels of phosphorylated eukaryotic initiation factor 2 alpha kinase(P-e IF2α),activating transcription factor 4(ATF4)and C/EBP homologous protein(CHOP),simultaneously enhancing the ratio of B-cell lymphoma 2(Bcl-2)to Bcl-2-associated X protein(Bax).These results suggested that Wuling powder could alleviate ER stress and inhibit cell apoptosis in the hippocampus by inhibiting protein translation and synthesis,thereby attenuating depressive-like behavior.
文摘清华大学哲学系卢风教授,系苏格兰圣安德鲁斯大学(1999年)、韩国延世大学(2004—2005年)和美国哈佛-燕京学社(2007年)访问学者,曾任清华大学哲学系主任、北京市政协委员、中国自然辩证法研究会环境哲学专业委员会主任等职,以及国内期刊《自然辩证法通讯》、《科学技术与辩证法》(后改名为《科学技术哲学研究》)和国际期刊《环境伦理学》(Environmental Ethics)、韩国期刊International Journal of Contextual Theology in East Asia等编委,现为清华大学生态文明研究中心研究员。他是国内最早关注生态文明的学者之一,多年来致力于环境伦理学、技术伦理学和生态哲学等领域的研究,曾主持完成国家社会科学基金重点项目“生态文明建设中的伦理问题研究”、一般项目“西方近现代价值观批判”以及多项省部级课题,出版学术专著《人类的家园——现代文化矛盾的哲学反思》(1996年)、《享乐与生存——现代人的生活方式与环境保护》(2000年)、《启蒙之后——近现代西方人价值追求的得与失》(2003年)、《应用伦理学与当代生活》(2004年)、《从现代文明到生态文明》(2009年)、《现代性与物欲的释放:杜维明先生访谈录》(2009年)、《人、环境与自然——环境哲学导论》(2011年)、《科技、自由与自然——科技伦理与环境伦理前沿问题研究》(2011年)、《非物质经济、文化与生态文明》(2015年)、《生态文明与美丽中国》(2019年)和《生态文明与生态哲学》(与王远哲合著,2022年)等多部,并公开发表学术论文300余篇。近期,《鄱阳湖学刊》编辑部王俊暐就生态文明的理论基石、研究方法和践行思路等问题与卢风教授展开了深入交流,现将访谈内容辑录如下,以飨读者。
