Prohibitin(PHB)plays critical roles in plant growth and development.In this study,we utilized CRISPR/Cas9 gene-editing technology to generate homozygous OsPHB2 knockout transgenic plants,designated cr-osphb2.The cr-os...Prohibitin(PHB)plays critical roles in plant growth and development.In this study,we utilized CRISPR/Cas9 gene-editing technology to generate homozygous OsPHB2 knockout transgenic plants,designated cr-osphb2.The cr-osphb2 line exhibited wider leaves,dwarfism,and shorter panicles.Subcellular localization results indicated that OsPHB2 localizes to mitochondria.Under salt stress conditions,cr-osphb2 exhibited enhanced tolerance.Haplotype(Hap)analysis identified three major Haps(Hap1,Hap2,and Hap3)of OsPHB2,among which Hap2 was associated with a greater number of effective panicles and higher yield,indicating its potential value for breeding applications.Collectively,our findings demonstrate that OsPHB2 plays an important role in regulating growth,development,and salt stress responses in rice.展开更多
基金supported by the Zhejiang Provincial Natural Science Outstanding Youth Fund Continuation Project,China(Grant No.LRG25C130002)the Innovation Program of the Chinese Academy of Agricultural Sciences(Grant No.CAAS-CSCB-202402)+3 种基金the Zhejiang Provincial Natural Science Foundation,China(Grant No.LD24C130001)the Biological Breeding-National Science and Technology Major Projects of China(Grant No.2023ZD04066)the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.Y2025YC96)the Agricultural Science and Technology Innovation Program,China(Grant No.CAAS-ASTIP-2021-CNRRI).
文摘Prohibitin(PHB)plays critical roles in plant growth and development.In this study,we utilized CRISPR/Cas9 gene-editing technology to generate homozygous OsPHB2 knockout transgenic plants,designated cr-osphb2.The cr-osphb2 line exhibited wider leaves,dwarfism,and shorter panicles.Subcellular localization results indicated that OsPHB2 localizes to mitochondria.Under salt stress conditions,cr-osphb2 exhibited enhanced tolerance.Haplotype(Hap)analysis identified three major Haps(Hap1,Hap2,and Hap3)of OsPHB2,among which Hap2 was associated with a greater number of effective panicles and higher yield,indicating its potential value for breeding applications.Collectively,our findings demonstrate that OsPHB2 plays an important role in regulating growth,development,and salt stress responses in rice.
