OBJECTIVE:To investigate the protective effect of the Chinese herbal formula of Jiedu Huayu decoction(解毒化瘀汤,JHD)on oral mucosa of rats with oral submucosal fibrosis(OSF)and its potential mechanism of action.METHO...OBJECTIVE:To investigate the protective effect of the Chinese herbal formula of Jiedu Huayu decoction(解毒化瘀汤,JHD)on oral mucosa of rats with oral submucosal fibrosis(OSF)and its potential mechanism of action.METHODS:Sprague-Dawley male OSF model rats were constructed by injection of betaine and topical rubbing and were randomly grouped and administered by gavage for 4 weeks.Mouth opening and buccal mucosa scores interleukin levels and the expression of Axin andβ-catenin proteins or genes were measured before and after drug administration.RESULTS:After treatment with JHD the buccal mucosal lesions of rats were significantly reduced Axin protein and mRNA expression were significantly increasedβ-catenin protein and m RNA expression were significantly decreased interleukin-1βand interleukin-6 levels were decreased and interleukin-10 levels were increased.CONCLUSION:The mechanism of action of JHD can effectively alleviate the pathological damage of buccal mucosa in OSF rats which may be related to the promotion of Axin expression and inhibition ofβ-catenin expression.展开更多
基金Funding from Scientific Research Project of Hunan Provincial Education Department:to Investigate the Mechanism of Blood-activating Circulation Through the Regulation of Blood Stasis and Detoxification based on Wntβ-catenin Signaling Pathway(No.21B0398)Study on the Epithelial Mesenchymal Transformation of Human Umbilical Vein Endothelial Cells Induced by Fuxin Formula(No.22C0190)Changsha City 2020 Annual Guiding Science and Technology Plan Projects:Effect of Blood Circulation and Detoxification Method on Epithelial Barrier Composition and Function of Oral Mucosa(No.kzd2001010)。
文摘OBJECTIVE:To investigate the protective effect of the Chinese herbal formula of Jiedu Huayu decoction(解毒化瘀汤,JHD)on oral mucosa of rats with oral submucosal fibrosis(OSF)and its potential mechanism of action.METHODS:Sprague-Dawley male OSF model rats were constructed by injection of betaine and topical rubbing and were randomly grouped and administered by gavage for 4 weeks.Mouth opening and buccal mucosa scores interleukin levels and the expression of Axin andβ-catenin proteins or genes were measured before and after drug administration.RESULTS:After treatment with JHD the buccal mucosal lesions of rats were significantly reduced Axin protein and mRNA expression were significantly increasedβ-catenin protein and m RNA expression were significantly decreased interleukin-1βand interleukin-6 levels were decreased and interleukin-10 levels were increased.CONCLUSION:The mechanism of action of JHD can effectively alleviate the pathological damage of buccal mucosa in OSF rats which may be related to the promotion of Axin expression and inhibition ofβ-catenin expression.
