Beta-cypermethrin is one of the synthetic pyrethroid,which is widely used to control insect pests and is found to be more effective than organphosphates and carbamates.However,it is also reported to be more toxic than...Beta-cypermethrin is one of the synthetic pyrethroid,which is widely used to control insect pests and is found to be more effective than organphosphates and carbamates.However,it is also reported to be more toxic than organphosphates and carbamates.It has been suggested that synthetic pyrethroids are readily absorbed by the gills of fish even at very low concentrations.Glutamic-pyruvic transaminase(GPT)and glutamic-oxaloacetic transaminase(GOT)exist in the cells of every tissue,particularly in the liver.If the liver is damaged,the enzymes are discharged from the cells into the serum.It increases the GPT and GOT metabolic activities quickly.It is,therefore,proposed that the effects of toxicants on serum GPT and GOT could be used as indexes in toxicological assessments.In this experiment,the effectiveness of beta-cypermethrin on the acute toxicity and on serum GPT and GOT concentration of crucian carp was studied.The LC 50 of beta-cypermethrin for crucian carp was 11.4μg/L.The crucian carp serum was treated against beta-cypermethrin levels of 0.114μg/L,0.57μg/L and 1.14μg/L for exposure times of 0d,5d,10d,15d and 20d,at all concentrations.The results show that when the beta-cypermethrin concentration increases and the exposure time is prolonged,the GPT and GOT activities of serum increased also.The results proved that beta-cypermethrin could cause great damage to crucian carp and its biochemical functions.展开更多
OBJECTIVE:To investigate the protective effect of the Chinese herbal formula of Jiedu Huayu decoction(解毒化瘀汤,JHD)on oral mucosa of rats with oral submucosal fibrosis(OSF)and its potential mechanism of action.METHO...OBJECTIVE:To investigate the protective effect of the Chinese herbal formula of Jiedu Huayu decoction(解毒化瘀汤,JHD)on oral mucosa of rats with oral submucosal fibrosis(OSF)and its potential mechanism of action.METHODS:Sprague-Dawley male OSF model rats were constructed by injection of betaine and topical rubbing and were randomly grouped and administered by gavage for 4 weeks.Mouth opening and buccal mucosa scores interleukin levels and the expression of Axin andβ-catenin proteins or genes were measured before and after drug administration.RESULTS:After treatment with JHD the buccal mucosal lesions of rats were significantly reduced Axin protein and mRNA expression were significantly increasedβ-catenin protein and m RNA expression were significantly decreased interleukin-1βand interleukin-6 levels were decreased and interleukin-10 levels were increased.CONCLUSION:The mechanism of action of JHD can effectively alleviate the pathological damage of buccal mucosa in OSF rats which may be related to the promotion of Axin expression and inhibition ofβ-catenin expression.展开更多
基金the National Basic Research Program(973 Program)(No.2001CCB00700)Wuhan Chenguang Scientific Program(No.2002500103)
文摘Beta-cypermethrin is one of the synthetic pyrethroid,which is widely used to control insect pests and is found to be more effective than organphosphates and carbamates.However,it is also reported to be more toxic than organphosphates and carbamates.It has been suggested that synthetic pyrethroids are readily absorbed by the gills of fish even at very low concentrations.Glutamic-pyruvic transaminase(GPT)and glutamic-oxaloacetic transaminase(GOT)exist in the cells of every tissue,particularly in the liver.If the liver is damaged,the enzymes are discharged from the cells into the serum.It increases the GPT and GOT metabolic activities quickly.It is,therefore,proposed that the effects of toxicants on serum GPT and GOT could be used as indexes in toxicological assessments.In this experiment,the effectiveness of beta-cypermethrin on the acute toxicity and on serum GPT and GOT concentration of crucian carp was studied.The LC 50 of beta-cypermethrin for crucian carp was 11.4μg/L.The crucian carp serum was treated against beta-cypermethrin levels of 0.114μg/L,0.57μg/L and 1.14μg/L for exposure times of 0d,5d,10d,15d and 20d,at all concentrations.The results show that when the beta-cypermethrin concentration increases and the exposure time is prolonged,the GPT and GOT activities of serum increased also.The results proved that beta-cypermethrin could cause great damage to crucian carp and its biochemical functions.
基金Funding from Scientific Research Project of Hunan Provincial Education Department:to Investigate the Mechanism of Blood-activating Circulation Through the Regulation of Blood Stasis and Detoxification based on Wntβ-catenin Signaling Pathway(No.21B0398)Study on the Epithelial Mesenchymal Transformation of Human Umbilical Vein Endothelial Cells Induced by Fuxin Formula(No.22C0190)Changsha City 2020 Annual Guiding Science and Technology Plan Projects:Effect of Blood Circulation and Detoxification Method on Epithelial Barrier Composition and Function of Oral Mucosa(No.kzd2001010)。
文摘OBJECTIVE:To investigate the protective effect of the Chinese herbal formula of Jiedu Huayu decoction(解毒化瘀汤,JHD)on oral mucosa of rats with oral submucosal fibrosis(OSF)and its potential mechanism of action.METHODS:Sprague-Dawley male OSF model rats were constructed by injection of betaine and topical rubbing and were randomly grouped and administered by gavage for 4 weeks.Mouth opening and buccal mucosa scores interleukin levels and the expression of Axin andβ-catenin proteins or genes were measured before and after drug administration.RESULTS:After treatment with JHD the buccal mucosal lesions of rats were significantly reduced Axin protein and mRNA expression were significantly increasedβ-catenin protein and m RNA expression were significantly decreased interleukin-1βand interleukin-6 levels were decreased and interleukin-10 levels were increased.CONCLUSION:The mechanism of action of JHD can effectively alleviate the pathological damage of buccal mucosa in OSF rats which may be related to the promotion of Axin expression and inhibition ofβ-catenin expression.
