目的研究淫羊藿女贞子配伍改善哮喘大鼠内源性糖皮质激素(Glucocorticoids,GC)抑制的作用机制。方法SD雄性大鼠,随机分为5组,分别是正常组、模型组、淫羊藿组、女贞子组、配伍组。卵蛋白致敏及激发建立大鼠哮喘模型,灌胃给予药物治疗2...目的研究淫羊藿女贞子配伍改善哮喘大鼠内源性糖皮质激素(Glucocorticoids,GC)抑制的作用机制。方法SD雄性大鼠,随机分为5组,分别是正常组、模型组、淫羊藿组、女贞子组、配伍组。卵蛋白致敏及激发建立大鼠哮喘模型,灌胃给予药物治疗2周。酶联免疫法检测大鼠血清促肾上腺皮质激素释放激素(Corticotropin-releasing hormone,CRH)、促肾上腺皮质激素(Adrenocorticotropic hormone,ACTH)、皮质酮(Corticosterone,CORT)水平,靶向代谢组学检测尿液内源性GC代谢物水平,免疫组化法检测肺羟基类固醇脱氢酶(Hydroxysteroid dehydrogenase,HSD)11B1、HSD11B2,及肾上腺细胞色素P450家族11亚家族B成员1(Cytochrome P450 family 11 subfamily B member 1,CYP11B1)、类固醇生成因子1(Steroid generating factor 1,SF1)的蛋白表达,蛋白免疫印迹法检测肝组织CYP11B1、SF1、HSD11B1、HSD11B2的蛋白表达。结果淫羊藿女贞子单用或合用能升高模型大鼠血清CRH、ACTH、CORT含量,改善尿液内源性GC代谢紊乱,上调肺组织HSD11B1、HSD11B2蛋白表达,肾上腺CYP11B1、SF1蛋白表达,肝组织HSD11B2、CYP11B1的蛋白表达。结论补肾药淫羊藿女贞子治疗哮喘的作用与调节下丘脑-垂体-肾上腺轴、调节GC合成或代谢途径关键因子表达、上调内源性GC水平,改善内源性GC紊乱有关。展开更多
The surface-enhanced Raman scattering(SERS) optical fiber probes were successfully prepared by self-assembling on polyelectrolyte multilayers. Gold nanorods(Au NRs) were used as SERS enhancement material to give excel...The surface-enhanced Raman scattering(SERS) optical fiber probes were successfully prepared by self-assembling on polyelectrolyte multilayers. Gold nanorods(Au NRs) were used as SERS enhancement material to give excellent biological affinity and stability to the SERS optical fiber probes. Au NRs were synthesized by seed growth method. The synergistic effect between AgNO_(3) and surfactant was investigated, and the highest yield was found when AgNO_(3) was 500 uL. Meanwhile, different SERS optical fiber probes were obtained by selecting silane coupling agent, polyelectrolyte multilayer and graphene oxide(GO) to treat quartz fiber. It was found that the SERS optical fiber probes obtained by the self-assembled on polyelectrolyte multilayers method performed better than those by other methods. In addition, Mapping was combined with finite element simulation to analyze the electromagnetic field distribution at the fiber end face.The electromagnetic field distribution of Au NRs was investigated, the difference of electromagnetic field intensity around the Au NRs with different arrangements was compared, the strongest signal was obtained when the Au NRs were head-to-head. Finally, sensitivity of the optimized SERS optical fiber probes could reach 10^(-9)mol/L, with excellent stability and repeatability.展开更多
We demonstrated a chemical process in the fabrication of a SERS fiber probe with an ultrahigh sensitivity.The synthesis was carried out by preparing Au@Ag core-shell nanorods (Au@Ag-NRs) selfassembled on polyelectroly...We demonstrated a chemical process in the fabrication of a SERS fiber probe with an ultrahigh sensitivity.The synthesis was carried out by preparing Au@Ag core-shell nanorods (Au@Ag-NRs) selfassembled on polyelectrolyte (PE) multilayers,for which Au@Ag-NRs were controlled by adjusting the silver layer thickness.The effect of silver layer thickness of Au@Ag-NRs on the SERS performance of the fiber probe was investigated.The SERS fiber probe shows the best performance when the silver layer thickness is controlled at 8.57 nm.Under the condition of optimizing silver layer thickness,the fiber probe exhibits ultra-high sensitivity (i e,10^(-10) M crystalline violet,CV),good reproducibility (i e,RSD of 3.5%) and stability.Besides,electromagnetic field distribution of the SERS fiber probe was also investigated.The strongest enhancement is found within the core of fiber,whereas a weakened electromagnetic field exists in the fiber cladding layer.The SERS fiber probe can be a good candidate in ultra-trace detection for biomedical and environmental areas.展开更多
文摘目的研究淫羊藿女贞子配伍改善哮喘大鼠内源性糖皮质激素(Glucocorticoids,GC)抑制的作用机制。方法SD雄性大鼠,随机分为5组,分别是正常组、模型组、淫羊藿组、女贞子组、配伍组。卵蛋白致敏及激发建立大鼠哮喘模型,灌胃给予药物治疗2周。酶联免疫法检测大鼠血清促肾上腺皮质激素释放激素(Corticotropin-releasing hormone,CRH)、促肾上腺皮质激素(Adrenocorticotropic hormone,ACTH)、皮质酮(Corticosterone,CORT)水平,靶向代谢组学检测尿液内源性GC代谢物水平,免疫组化法检测肺羟基类固醇脱氢酶(Hydroxysteroid dehydrogenase,HSD)11B1、HSD11B2,及肾上腺细胞色素P450家族11亚家族B成员1(Cytochrome P450 family 11 subfamily B member 1,CYP11B1)、类固醇生成因子1(Steroid generating factor 1,SF1)的蛋白表达,蛋白免疫印迹法检测肝组织CYP11B1、SF1、HSD11B1、HSD11B2的蛋白表达。结果淫羊藿女贞子单用或合用能升高模型大鼠血清CRH、ACTH、CORT含量,改善尿液内源性GC代谢紊乱,上调肺组织HSD11B1、HSD11B2蛋白表达,肾上腺CYP11B1、SF1蛋白表达,肝组织HSD11B2、CYP11B1的蛋白表达。结论补肾药淫羊藿女贞子治疗哮喘的作用与调节下丘脑-垂体-肾上腺轴、调节GC合成或代谢途径关键因子表达、上调内源性GC水平,改善内源性GC紊乱有关。
基金Funded by National Natural Science Foundation of China (Nos.51372179, 51772224)the Open Projects Foundation of Yangtze Optical Fiber and Cable Joint Stock Limited Company (YOFC)(No.SKLD1705)。
文摘The surface-enhanced Raman scattering(SERS) optical fiber probes were successfully prepared by self-assembling on polyelectrolyte multilayers. Gold nanorods(Au NRs) were used as SERS enhancement material to give excellent biological affinity and stability to the SERS optical fiber probes. Au NRs were synthesized by seed growth method. The synergistic effect between AgNO_(3) and surfactant was investigated, and the highest yield was found when AgNO_(3) was 500 uL. Meanwhile, different SERS optical fiber probes were obtained by selecting silane coupling agent, polyelectrolyte multilayer and graphene oxide(GO) to treat quartz fiber. It was found that the SERS optical fiber probes obtained by the self-assembled on polyelectrolyte multilayers method performed better than those by other methods. In addition, Mapping was combined with finite element simulation to analyze the electromagnetic field distribution at the fiber end face.The electromagnetic field distribution of Au NRs was investigated, the difference of electromagnetic field intensity around the Au NRs with different arrangements was compared, the strongest signal was obtained when the Au NRs were head-to-head. Finally, sensitivity of the optimized SERS optical fiber probes could reach 10^(-9)mol/L, with excellent stability and repeatability.
基金Funded by National Natural Science Foundation of China (Nos.51372179, 51772224)the Open Projects Foundation of Yangtze Optical Fiber and Cable Joint Stock Limited Company (YOFC)(No.SKLD1705)。
文摘We demonstrated a chemical process in the fabrication of a SERS fiber probe with an ultrahigh sensitivity.The synthesis was carried out by preparing Au@Ag core-shell nanorods (Au@Ag-NRs) selfassembled on polyelectrolyte (PE) multilayers,for which Au@Ag-NRs were controlled by adjusting the silver layer thickness.The effect of silver layer thickness of Au@Ag-NRs on the SERS performance of the fiber probe was investigated.The SERS fiber probe shows the best performance when the silver layer thickness is controlled at 8.57 nm.Under the condition of optimizing silver layer thickness,the fiber probe exhibits ultra-high sensitivity (i e,10^(-10) M crystalline violet,CV),good reproducibility (i e,RSD of 3.5%) and stability.Besides,electromagnetic field distribution of the SERS fiber probe was also investigated.The strongest enhancement is found within the core of fiber,whereas a weakened electromagnetic field exists in the fiber cladding layer.The SERS fiber probe can be a good candidate in ultra-trace detection for biomedical and environmental areas.
