The microbial population dynamics in bulk and developing cucumber rhizospheres were studiedby cultivation and cultivation-independent approach based on directly extracted DNA toprovide baseline data. Soil and rhizosph...The microbial population dynamics in bulk and developing cucumber rhizospheres were studiedby cultivation and cultivation-independent approach based on directly extracted DNA toprovide baseline data. Soil and rhizosphere samples were taken from tested field 2, 4, 7 and10 weeks after the seeds were planted, which was positively related to the corresponding dateof cucumber growth stages. The plate culture amount showed that total number of bacteria,fungi and actinomyces began to rise when cucumber planted and quickly reached peak at seedlingor blossom period, but decreased slightly later. Bacterial population in rhizosphere washigher by comparison with that of counterpart except for seedling and flowering stages, butthe shift trend of them were quite similar all the time. Nitrogen fixating, nitrobacter andammonifying bacteria showed the same change tendency in population as bacteria and actinomycesdid, however, cellulose-decomposing bacteria had the contrary rhizosphere effect as cucumberdeveloped. Data revealed that positive relevance existed between the dominant rhizospheremicrobe population and cucumber development. PCR was employed to amplify the V3 region of 16SrDNA, then the products were subjected to denaturing gradient gel electrophoresis(DGGE). DGGEprofile indicated that a few microbe species lived stable in farmland soil, but some wereinfluenced by population due to cucumber roots growth. Significant difference was observed inbulk and rhizosphere soils especially for the seedling and flowering samples. Few prominentbands in DGGE patterns, which displayed stronger or less illumination, means the representativebacteria had great population variation in that period. These phenomena indicated thatflowering cucumber heavily affected rhizosphere bacteria, or the bacteria, most probably theuncultured bacteria, functioned specially to cucumber blossom. Most detected bands with noillumination change in DGGE quite possibly represent the indigenous microbes that wereessential for constructing and stabilizing farmland microecological environment.展开更多
Objective: to explore the specific effect, degree and possible mechanism of biofeedback electrical stimulation intervention on the expression of characteristic indicators in Pelvic floor dysfunction (PFD) rats. Method...Objective: to explore the specific effect, degree and possible mechanism of biofeedback electrical stimulation intervention on the expression of characteristic indicators in Pelvic floor dysfunction (PFD) rats. Methods: thirty female rats of childbearing age were successfully modeled as PFD rats. They were all divided into blank control group (lidocaine intervention), conditional control group (EERK agonist intervention) and observation group (biofeedback electrical stimulation intervention). Each group had 10 rats. The change range of each characteristic index (MMP-2, HA, LN, IV-C, DES, Elafin) before and after each intervention was recorded and calculated, and the statistical difference of each index change range was compared. Results: after the intervention, the change range of each characteristic index (MMP, ha, LN, IV-C, DES, Elafin) was compared with that before the intervention, from high to low: observation group, conditional control group, blank control group, the difference among the three groups of rats was statistically significant (P<0.05). After the intervention, 8 of the 10 rats in the observation group improved, while the other 2 rats were ineffective. Comparing the change range of each index between the effective group and the ineffective group, the differences of MMP, ha, LN, IV-C, DES and Elafin were statistically significant (P<0.05). The influence of each characteristic index on the prognosis of rats from high to low is: HA(OR4.22), DES(OR4.06), MMP(OR3.86), Elafin(OR3.60), IV-C(OR3.53), LN(OR2.97). Conclusion: compared with drug injection, biofeedback electrical stimulation can better control the local inflammation of the lesion and promote the expression of repair protein.展开更多
Using a 34 years database consisting of sea level pressure and sea iceconcentration, the relationship between Antarctic Oscillation (AAO) and Antarctic Sea-iceOscillation (ASO) was investigated. Firstly, Empirical Ort...Using a 34 years database consisting of sea level pressure and sea iceconcentration, the relationship between Antarctic Oscillation (AAO) and Antarctic Sea-iceOscillation (ASO) was investigated. Firstly, Empirical Orthogonal Function (EOF) analysis depictsthe spatial variability of AAO and ASO and Antarctic Sea-ice Oscillation Index (ASOI) was definedfor the first time. Secondly, power spectrum and head-lag correlation analysis show that ASO and AAOhave the same periods of quasi-three years, quasi-one year and quasi-half a year. Corresponding toAAO, ASO has an evident anti-oscillation after one and half a year.展开更多
文摘The microbial population dynamics in bulk and developing cucumber rhizospheres were studiedby cultivation and cultivation-independent approach based on directly extracted DNA toprovide baseline data. Soil and rhizosphere samples were taken from tested field 2, 4, 7 and10 weeks after the seeds were planted, which was positively related to the corresponding dateof cucumber growth stages. The plate culture amount showed that total number of bacteria,fungi and actinomyces began to rise when cucumber planted and quickly reached peak at seedlingor blossom period, but decreased slightly later. Bacterial population in rhizosphere washigher by comparison with that of counterpart except for seedling and flowering stages, butthe shift trend of them were quite similar all the time. Nitrogen fixating, nitrobacter andammonifying bacteria showed the same change tendency in population as bacteria and actinomycesdid, however, cellulose-decomposing bacteria had the contrary rhizosphere effect as cucumberdeveloped. Data revealed that positive relevance existed between the dominant rhizospheremicrobe population and cucumber development. PCR was employed to amplify the V3 region of 16SrDNA, then the products were subjected to denaturing gradient gel electrophoresis(DGGE). DGGEprofile indicated that a few microbe species lived stable in farmland soil, but some wereinfluenced by population due to cucumber roots growth. Significant difference was observed inbulk and rhizosphere soils especially for the seedling and flowering samples. Few prominentbands in DGGE patterns, which displayed stronger or less illumination, means the representativebacteria had great population variation in that period. These phenomena indicated thatflowering cucumber heavily affected rhizosphere bacteria, or the bacteria, most probably theuncultured bacteria, functioned specially to cucumber blossom. Most detected bands with noillumination change in DGGE quite possibly represent the indigenous microbes that wereessential for constructing and stabilizing farmland microecological environment.
文摘Objective: to explore the specific effect, degree and possible mechanism of biofeedback electrical stimulation intervention on the expression of characteristic indicators in Pelvic floor dysfunction (PFD) rats. Methods: thirty female rats of childbearing age were successfully modeled as PFD rats. They were all divided into blank control group (lidocaine intervention), conditional control group (EERK agonist intervention) and observation group (biofeedback electrical stimulation intervention). Each group had 10 rats. The change range of each characteristic index (MMP-2, HA, LN, IV-C, DES, Elafin) before and after each intervention was recorded and calculated, and the statistical difference of each index change range was compared. Results: after the intervention, the change range of each characteristic index (MMP, ha, LN, IV-C, DES, Elafin) was compared with that before the intervention, from high to low: observation group, conditional control group, blank control group, the difference among the three groups of rats was statistically significant (P<0.05). After the intervention, 8 of the 10 rats in the observation group improved, while the other 2 rats were ineffective. Comparing the change range of each index between the effective group and the ineffective group, the differences of MMP, ha, LN, IV-C, DES and Elafin were statistically significant (P<0.05). The influence of each characteristic index on the prognosis of rats from high to low is: HA(OR4.22), DES(OR4.06), MMP(OR3.86), Elafin(OR3.60), IV-C(OR3.53), LN(OR2.97). Conclusion: compared with drug injection, biofeedback electrical stimulation can better control the local inflammation of the lesion and promote the expression of repair protein.
文摘Using a 34 years database consisting of sea level pressure and sea iceconcentration, the relationship between Antarctic Oscillation (AAO) and Antarctic Sea-iceOscillation (ASO) was investigated. Firstly, Empirical Orthogonal Function (EOF) analysis depictsthe spatial variability of AAO and ASO and Antarctic Sea-ice Oscillation Index (ASOI) was definedfor the first time. Secondly, power spectrum and head-lag correlation analysis show that ASO and AAOhave the same periods of quasi-three years, quasi-one year and quasi-half a year. Corresponding toAAO, ASO has an evident anti-oscillation after one and half a year.
