探讨了沙质土壤条件下不同氮肥施用水平对油莎豆干物质及养分累积、分配和产量的影响,为沙质土壤条件下油莎豆合理施用氮肥提供理论依据。以油莎豆品种“中油莎1号”为试验材料,采用大田试验,设不施氮(N0)、低氮(N1)、中氮(N2)、高氮(N...探讨了沙质土壤条件下不同氮肥施用水平对油莎豆干物质及养分累积、分配和产量的影响,为沙质土壤条件下油莎豆合理施用氮肥提供理论依据。以油莎豆品种“中油莎1号”为试验材料,采用大田试验,设不施氮(N0)、低氮(N1)、中氮(N2)、高氮(N3)4个施氮水平,分析不同氮肥施用水平对油莎豆干物质及养分累积、分配和产量的影响。结果表明,随生育期的推进油莎豆茎叶、根、块茎干物质累积量的变化趋势均呈上升趋势。在块茎形成期前油莎豆主要以地上部茎叶营养生长为主,块茎形成期后生长中心逐渐向地下部块茎转移,至收获期块茎干物质累积分配比例高达43.34%~51.00%。2年N2水平块茎干物质累积量及分配比例均高于其他水平,较其他施氮水平平均提高17.50%和4.00%。油莎豆对氮、钾需求量较大,磷较少。收获期油莎豆氮、磷、钾累积量表现为块茎>茎叶>根,块茎成为油莎豆氮磷钾最终的贮藏器官。N2水平下油莎豆氮、磷、钾累积吸收量在整个生育期内一直呈较高水平,特别是块茎在收获期的氮、磷、钾分配比例分别达到47.09%、65.82%和56.40%。N2水平下,油莎豆地上部生物量、地下部生物量、千粒重、块茎产量均高于其他水平,较其他施氮水平平均提高5.57%、7.40%、15.83%和18.12%。在沙质土壤栽培条件下,油莎豆氮施肥量以150 kg hm^(-2)较为适宜。展开更多
目的研究miR-142-3p在血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞肥大中的作用和机制。方法首先,采用AngⅡ诱导心肌细胞肥大,实时定量PCR检测miR-142-3p表达是否下调;其次,将实验分为对照组、AngⅡ诱导的心肌细胞肥大组(简称AngⅡ组)和AngⅡ诱...目的研究miR-142-3p在血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞肥大中的作用和机制。方法首先,采用AngⅡ诱导心肌细胞肥大,实时定量PCR检测miR-142-3p表达是否下调;其次,将实验分为对照组、AngⅡ诱导的心肌细胞肥大组(简称AngⅡ组)和AngⅡ诱导心肌细胞肥大同时转染miR-142-3p mimics组(简称AngⅡ+miR-142-3p mimics组),实验均重复3次。使用miR-142-3p模拟物(miR-142-3p mimics)转染原代乳鼠心肌细胞8 h,后用AngⅡ诱导上述细胞48 h,使用PCR检测各组miR-142-3p,p300 mRNA表达以及心肌肥厚标记物心房钠尿肽(ANP)、脑钠肽(BNP)、β-肌球蛋白重链(β-MHC)的m RNA表达,使用Western blotting法检测相关蛋白表达,α-actinin染色观察心肌细胞大小。结果细胞肥大模型构建成功后,与对照组比较,AngⅡ组细胞中miR-142-3p表达显著减少[(1.224±0.04) vs(0.618±0.035)],p300表达明显增多[(1.013±0.009) vs (1.656±0.063)],差异均有统计学意义(P<0.05);miRNA模拟物转染成功后,与AngⅡ组比较,AngⅡ+miR-142-3p mimics组ANP、BNP、β-MHC和Caspase-3表达明显减少[(1.947±0.125) vs (1.138±0.086)、(2.323±0.110) vs (1.26±0.066)、(1.333±0.103) vs (0.840±0.053)、(1.822±0.250) vs (1.163±0.143)],p300表达明显减少[(0.933±0.038) vs (0.603±0.038)],差异均有统计学意义(P<0.05)。结论 miR-142-3p可能通过抑制p300基因的表达来发挥对心肌细胞肥大的保护作用。展开更多
Primary school Chinese is a very important basic subject. Composition teaching is the key and difficult point in Chinese teaching. Pupils are in the initial stage of language learning, and the accumulation of vocabula...Primary school Chinese is a very important basic subject. Composition teaching is the key and difficult point in Chinese teaching. Pupils are in the initial stage of language learning, and the accumulation of vocabulary and reading is still relatively limited. They are prone to fear difficulties in writing. For primary school students, the time to learn Chinese is still relatively short, both in terms of vocabulary and reading volume, is still relatively inadequate. At the beginning of learning to write, it is easy to have difficulties in expression or words, and the frustration in learning is obvious. Therefore, in the teaching of writing, teachers must pay attention to the innovation of methods, constantly explore new teaching designs, enhance students interest in learning and reduce students writing obstacles. Next, the article starts thinking about innovating the teaching method of Chinese composition in primary schools.展开更多
文摘探讨了沙质土壤条件下不同氮肥施用水平对油莎豆干物质及养分累积、分配和产量的影响,为沙质土壤条件下油莎豆合理施用氮肥提供理论依据。以油莎豆品种“中油莎1号”为试验材料,采用大田试验,设不施氮(N0)、低氮(N1)、中氮(N2)、高氮(N3)4个施氮水平,分析不同氮肥施用水平对油莎豆干物质及养分累积、分配和产量的影响。结果表明,随生育期的推进油莎豆茎叶、根、块茎干物质累积量的变化趋势均呈上升趋势。在块茎形成期前油莎豆主要以地上部茎叶营养生长为主,块茎形成期后生长中心逐渐向地下部块茎转移,至收获期块茎干物质累积分配比例高达43.34%~51.00%。2年N2水平块茎干物质累积量及分配比例均高于其他水平,较其他施氮水平平均提高17.50%和4.00%。油莎豆对氮、钾需求量较大,磷较少。收获期油莎豆氮、磷、钾累积量表现为块茎>茎叶>根,块茎成为油莎豆氮磷钾最终的贮藏器官。N2水平下油莎豆氮、磷、钾累积吸收量在整个生育期内一直呈较高水平,特别是块茎在收获期的氮、磷、钾分配比例分别达到47.09%、65.82%和56.40%。N2水平下,油莎豆地上部生物量、地下部生物量、千粒重、块茎产量均高于其他水平,较其他施氮水平平均提高5.57%、7.40%、15.83%和18.12%。在沙质土壤栽培条件下,油莎豆氮施肥量以150 kg hm^(-2)较为适宜。
文摘目的研究miR-142-3p在血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞肥大中的作用和机制。方法首先,采用AngⅡ诱导心肌细胞肥大,实时定量PCR检测miR-142-3p表达是否下调;其次,将实验分为对照组、AngⅡ诱导的心肌细胞肥大组(简称AngⅡ组)和AngⅡ诱导心肌细胞肥大同时转染miR-142-3p mimics组(简称AngⅡ+miR-142-3p mimics组),实验均重复3次。使用miR-142-3p模拟物(miR-142-3p mimics)转染原代乳鼠心肌细胞8 h,后用AngⅡ诱导上述细胞48 h,使用PCR检测各组miR-142-3p,p300 mRNA表达以及心肌肥厚标记物心房钠尿肽(ANP)、脑钠肽(BNP)、β-肌球蛋白重链(β-MHC)的m RNA表达,使用Western blotting法检测相关蛋白表达,α-actinin染色观察心肌细胞大小。结果细胞肥大模型构建成功后,与对照组比较,AngⅡ组细胞中miR-142-3p表达显著减少[(1.224±0.04) vs(0.618±0.035)],p300表达明显增多[(1.013±0.009) vs (1.656±0.063)],差异均有统计学意义(P<0.05);miRNA模拟物转染成功后,与AngⅡ组比较,AngⅡ+miR-142-3p mimics组ANP、BNP、β-MHC和Caspase-3表达明显减少[(1.947±0.125) vs (1.138±0.086)、(2.323±0.110) vs (1.26±0.066)、(1.333±0.103) vs (0.840±0.053)、(1.822±0.250) vs (1.163±0.143)],p300表达明显减少[(0.933±0.038) vs (0.603±0.038)],差异均有统计学意义(P<0.05)。结论 miR-142-3p可能通过抑制p300基因的表达来发挥对心肌细胞肥大的保护作用。
文摘Primary school Chinese is a very important basic subject. Composition teaching is the key and difficult point in Chinese teaching. Pupils are in the initial stage of language learning, and the accumulation of vocabulary and reading is still relatively limited. They are prone to fear difficulties in writing. For primary school students, the time to learn Chinese is still relatively short, both in terms of vocabulary and reading volume, is still relatively inadequate. At the beginning of learning to write, it is easy to have difficulties in expression or words, and the frustration in learning is obvious. Therefore, in the teaching of writing, teachers must pay attention to the innovation of methods, constantly explore new teaching designs, enhance students interest in learning and reduce students writing obstacles. Next, the article starts thinking about innovating the teaching method of Chinese composition in primary schools.
