To investigate the function of the zinc finger protein BnZAT12 in Brassica napus,bioinformatics analysis was conducted on BnZAT12.The results showed that the open reading frame of BnZAT12 was 477 bp in length,encoding...To investigate the function of the zinc finger protein BnZAT12 in Brassica napus,bioinformatics analysis was conducted on BnZAT12.The results showed that the open reading frame of BnZAT12 was 477 bp in length,encoding 158 amino acid residues.The deduced protein had a molecular weight of 16864.72 Da and a theoretical isoelectric point of 9.68.The phylogenetic tree showed that Brassica napus had the closest relationship with Brassica oleracea belonging to Brassicaceae and the farthest relationship with Oryza sativa.The analysis of the promoter region suggested that BnZAT12 may be regulated by factors such as light,abscisic acid,and methyl jasmonate.Furthermore,the BnZAT12 overexpression vector was constructed by seamless cloning.This study laid a foundation of molecular biology for further elucidating the role of BnZAT12.展开更多
文摘To investigate the function of the zinc finger protein BnZAT12 in Brassica napus,bioinformatics analysis was conducted on BnZAT12.The results showed that the open reading frame of BnZAT12 was 477 bp in length,encoding 158 amino acid residues.The deduced protein had a molecular weight of 16864.72 Da and a theoretical isoelectric point of 9.68.The phylogenetic tree showed that Brassica napus had the closest relationship with Brassica oleracea belonging to Brassicaceae and the farthest relationship with Oryza sativa.The analysis of the promoter region suggested that BnZAT12 may be regulated by factors such as light,abscisic acid,and methyl jasmonate.Furthermore,the BnZAT12 overexpression vector was constructed by seamless cloning.This study laid a foundation of molecular biology for further elucidating the role of BnZAT12.
