OBJECTIVE:To investigate whether electroacupuncture(EA)therapy for overactive bladder(OAB)exerts its effect by modulating mechanosensitive channels in the urothelium,thereby improving bladder sensory function.METHODS:...OBJECTIVE:To investigate whether electroacupuncture(EA)therapy for overactive bladder(OAB)exerts its effect by modulating mechanosensitive channels in the urothelium,thereby improving bladder sensory function.METHODS:In this study,a rat model of OAB was created by using intraperitoneal injections of cyclophosphamide.We performed either EA or bladder perfusion with HC-067047[a transient receptor potential vanilloid 4(TRPV4)antagonist]and assessed the efficacy of electroacupuncture in the treatment of OAB in rats via urodynamic determination and Void spot assay.tissue morphology,distribution and expression of the TRPV4protein and the amount of adenosine triphosphate(ATP)and Ca2+released from urothelial cells in each group of rats were observed to identify the mechanism by which electroacupuncture improves OAB in rats.RESULTS:EA ameliorated bladder function and voiding behaviour,improved bladder uroepithelial tissue morphology,and significantly reduced the immunofluorescence intensity and the mRNA and protein expression levels of TRPV4 in the uroepithelium of OAB rats.Moreover,the simulated mechanical stimulationinduced increases in Ca^(2+)concentration and the release of ATP and acetylcholine(Ach)from bladder urothelial cells were inhibited.The changes in EA followed the same trend as those in HC-067047.CONCLUSIONS:These results suggest that EA inhibits bladder sensory function by downregulating the expression of mechanically activated TRPV4 ion channels distributed in bladder urothelial cells,which correspondingly decreases the inward flow of extracellular Ca^(2+)and reduces the release of ATP and Ach,thereby attenuating excitatory signals.展开更多
为探究南水北调中线工程蓄水后新水域环境对鱼类群落的影响,采用eDNA宏条形码技术(environmental DNA metabarcoding)首次对密云水库鱼类群落结构及多样性的时空变化特征进行了分析,并结合生态位宽度Shannon-Winner(B_(i))和重叠Pianka...为探究南水北调中线工程蓄水后新水域环境对鱼类群落的影响,采用eDNA宏条形码技术(environmental DNA metabarcoding)首次对密云水库鱼类群落结构及多样性的时空变化特征进行了分析,并结合生态位宽度Shannon-Winner(B_(i))和重叠Pianka指数(Q_(ik))研究了鱼类种群间的相互作用、共生共存及生态位分化的响应过程。结果表明:本次调查密云水库共检出86个鱼类物种eDNA信息,隶属14目21科68属,有3个鱼类eDNA信息仅注释到科、属级分类水平;鱼类主要栖息于水库底层(61.63%),以肉食性(43.02%)和杂食性(26.74%)摄食类型为主;PCoA分析显示,密云水库各采样点鱼类群落结构无显著性差异(P>0.05),Alpha多样性各项指数也无显著性差异(P>0.05);主要鱼类生态位宽度为1.15~9.25,均为中生态和广生态位种,其中,生态位重叠指数Q_(ik)>0.6的种对有517对,约占总种对数的14.15%;与历史资料相比,鲢(Hypophthalmichthys molitrix)、鳙(Aristichthys nobilis)、鲫(Carassius auratus)、鲂(Megalobrama terminalis)和鲤(Cyprinus carpio)等增殖放流鱼类仍为水库主要经济鱼类。研究表明,蓄水后营底栖、以肉食性和草食性为主要生活策略的水库鱼类对环境的适应能力较弱,且彼此之间竞争明显。展开更多
基金Natural Science Foundation-funded Project:Research on the Effect of Acupuncture in Regulating Bladder Excitability based on the Generation and Transmission of Afferent Information from Urinary Control(No.82174516)Investigation into the Mechanism of Acupuncture-regulated Bladder Excitability based on Sensory Nerve Mechanotransduction Signaling(No.82474645)。
文摘OBJECTIVE:To investigate whether electroacupuncture(EA)therapy for overactive bladder(OAB)exerts its effect by modulating mechanosensitive channels in the urothelium,thereby improving bladder sensory function.METHODS:In this study,a rat model of OAB was created by using intraperitoneal injections of cyclophosphamide.We performed either EA or bladder perfusion with HC-067047[a transient receptor potential vanilloid 4(TRPV4)antagonist]and assessed the efficacy of electroacupuncture in the treatment of OAB in rats via urodynamic determination and Void spot assay.tissue morphology,distribution and expression of the TRPV4protein and the amount of adenosine triphosphate(ATP)and Ca2+released from urothelial cells in each group of rats were observed to identify the mechanism by which electroacupuncture improves OAB in rats.RESULTS:EA ameliorated bladder function and voiding behaviour,improved bladder uroepithelial tissue morphology,and significantly reduced the immunofluorescence intensity and the mRNA and protein expression levels of TRPV4 in the uroepithelium of OAB rats.Moreover,the simulated mechanical stimulationinduced increases in Ca^(2+)concentration and the release of ATP and acetylcholine(Ach)from bladder urothelial cells were inhibited.The changes in EA followed the same trend as those in HC-067047.CONCLUSIONS:These results suggest that EA inhibits bladder sensory function by downregulating the expression of mechanically activated TRPV4 ion channels distributed in bladder urothelial cells,which correspondingly decreases the inward flow of extracellular Ca^(2+)and reduces the release of ATP and Ach,thereby attenuating excitatory signals.
