目的建立饱和盐辅助-分散液液微萃取(saturation salt assisted-dispersible liquid-liquid microextraction,SSA-DLLME)快速样品前处理,气相色谱-串联质谱法(gas chromatography-tandem mass spectrometry,GC-MS/MS)检测啤酒中氨基甲...目的建立饱和盐辅助-分散液液微萃取(saturation salt assisted-dispersible liquid-liquid microextraction,SSA-DLLME)快速样品前处理,气相色谱-串联质谱法(gas chromatography-tandem mass spectrometry,GC-MS/MS)检测啤酒中氨基甲酸乙酯和9种挥发性亚硝胺包括N-亚硝基二甲胺、N-亚硝基二正丙胺、N-亚硝基二乙胺、N-亚硝基哌啶、N-亚硝基吡咯烷、N-亚硝基吗啉、N-亚硝基甲乙胺、N-亚硝基二丁胺和N-亚硝基二苯胺的检测方法。方法样品经甲醇提取,加入稳定同位素标记的目标物定量,在饱和碳酸钠辅助萃取下,猛力注入二氯甲烷进行SSA-DLLME,离心,取下层有机相进样,多反应监测(multiple reaction monitoring,MRM)模式进行GC-MS/MS检测,内标法定量。结果该方法在考察的浓度范围内线性良好(r^(2)>0.9970),方法检出限(limits of detection,LODs)为0.10~0.34μg/L,定量限(limits of quantitation,LOQs)为0.34~1.15μg/L;3水平(1、5、10μg/L)加标回收率为81.98%~123.50%,相对标准偏差(relative standard deviations,RSDs)为0.57%~13.04%(n=8)。结论该方法极为简便、快捷,检出限低且准确度好,可满足啤酒中氨基甲酸乙酯和9种挥发性亚硝胺快速筛查检测的需要。展开更多
The quality of skeleton system for the cheetah robot goes hand in hand with its bionic result of its shape, structure and functions. In view of the skeleton system constitution and structural characteristic of the che...The quality of skeleton system for the cheetah robot goes hand in hand with its bionic result of its shape, structure and functions. In view of the skeleton system constitution and structural characteristic of the cheetah, the team applied structure design, stimulation analysis and parameter optimization to developing the cheetah robot. In addition, after the invention of cheetah robot＇s anterior lumbar vertebra based on its functional attribute and connectivity attribute, the Solidworks Simulation was utilized to analyze the design, according to which improvement on the lumbar vertebra was made. Plus, the advantages of the CAD and CAE made the high efficiency of design work and high quality of the cheetah robot possible.展开更多
文摘目的建立饱和盐辅助-分散液液微萃取(saturation salt assisted-dispersible liquid-liquid microextraction,SSA-DLLME)快速样品前处理,气相色谱-串联质谱法(gas chromatography-tandem mass spectrometry,GC-MS/MS)检测啤酒中氨基甲酸乙酯和9种挥发性亚硝胺包括N-亚硝基二甲胺、N-亚硝基二正丙胺、N-亚硝基二乙胺、N-亚硝基哌啶、N-亚硝基吡咯烷、N-亚硝基吗啉、N-亚硝基甲乙胺、N-亚硝基二丁胺和N-亚硝基二苯胺的检测方法。方法样品经甲醇提取,加入稳定同位素标记的目标物定量,在饱和碳酸钠辅助萃取下,猛力注入二氯甲烷进行SSA-DLLME,离心,取下层有机相进样,多反应监测(multiple reaction monitoring,MRM)模式进行GC-MS/MS检测,内标法定量。结果该方法在考察的浓度范围内线性良好(r^(2)>0.9970),方法检出限(limits of detection,LODs)为0.10~0.34μg/L,定量限(limits of quantitation,LOQs)为0.34~1.15μg/L;3水平(1、5、10μg/L)加标回收率为81.98%~123.50%,相对标准偏差(relative standard deviations,RSDs)为0.57%~13.04%(n=8)。结论该方法极为简便、快捷,检出限低且准确度好,可满足啤酒中氨基甲酸乙酯和9种挥发性亚硝胺快速筛查检测的需要。
文摘The quality of skeleton system for the cheetah robot goes hand in hand with its bionic result of its shape, structure and functions. In view of the skeleton system constitution and structural characteristic of the cheetah, the team applied structure design, stimulation analysis and parameter optimization to developing the cheetah robot. In addition, after the invention of cheetah robot＇s anterior lumbar vertebra based on its functional attribute and connectivity attribute, the Solidworks Simulation was utilized to analyze the design, according to which improvement on the lumbar vertebra was made. Plus, the advantages of the CAD and CAE made the high efficiency of design work and high quality of the cheetah robot possible.
