Based on the information from forest resources distribution maps of Luoning County of 1983 and 1999, six indices were used to analyze spatial patterns and dynamics of forest landscapes of t...Based on the information from forest resources distribution maps of Luoning County of 1983 and 1999, six indices were used to analyze spatial patterns and dynamics of forest landscapes of the typical region in the middle of the Yihe-Luohe river basin. These indices include patch number, mean patch area, fragment index, patch extension index, etc. The results showed that: (1) There was a rapid increase in the number of patch and total area from 1983 to 1999 in the study area. The fragment degree became very high. (2) The area of all the forest patch types had witnessed great changes. The fractal degree of each forest patch type became big from 1983 to 1999. The mean extension index of Robinia pseudoacacia forest, non-forest, shrub forest, sparse forest, and Quercus species forest increased rapidly, but that of economic forest became zero. The fractal dimension each showed that forest coverage has been promoted. (3) The changes of landscape patterns were different in different geomorphic regions. From 1983 to 1999 the vegetation cover area, the gross number and the density of patch, diversity and evenness of landscape were all reduced greatly in gullies and ravines, but the maximum area and the mean area of patch types were increased. In hilly region, both the forest cover area and the number of patch increased from 1983 to 1999, but the mean area of patch was reduced greatly. In mountain region, even though the area under forest canopy reduced from 1983 to 1999, the patch number was increased greatly, the mean area of all patch types was reduced, the extension index, diversity index and evenness index of landscape were all increased. Furthermore, because of different types of land use, human activity and terrain, the vegetation changes on northern and southern mountain slopes were different. According to these analyses, the main driving forces, such as the policies of management, market economy, influence of human activities etc. are brought out.展开更多
Human extracellular superoxide dismutase(hEC-SOD) is a secreted tetrameric protein involved in the protection of a human body from oxygen free radicals. Its three-dimensional structure has not been confirmed. hEC-SOD ...Human extracellular superoxide dismutase(hEC-SOD) is a secreted tetrameric protein involved in the protection of a human body from oxygen free radicals. Its three-dimensional structure has not been confirmed. hEC-SOD couldn′t be expressed in E.coli. We constructed a hybrid enzyme, which comprises the N-terminal and C-terminal domains from hEC-SOD, fused it to human Cu,Zn-SOD. The hybrid enzyme is expressed successfully in E.coli. Further, we analyzed the expression of hEC-SOD in E.coli by mRNA differential displaying.展开更多
To enhance the relative movement of domains, we inserted a random sequence of fifteen-peptide into the three domains of L-aspartase. By means of directed screening, the three isoforms of monomeric, dimmeric and tetram...To enhance the relative movement of domains, we inserted a random sequence of fifteen-peptide into the three domains of L-aspartase. By means of directed screening, the three isoforms of monomeric, dimmeric and tetrameric enzymes were obtained. Compared to the wild-type tetrameric L-asparease, these mutants remained 19.7%, 42.3%, and 92% of the enzyme activity, respectively. Moreover, the examination of enzyme properties revealed that their k_ cat and K_M changed in varying degrees, and the optimum pH shifted towards acidic pH, while the dependence of the activity of enzyme on Mg 2+ concentration and thermostability increased. Therefore this strategy provides a novel approach to directed evolution of enzymes.展开更多
基金Prominent Youth Science Foundation of Henan Province No.0003+2 种基金 No.9920 Natural Science Foundation of Henan Province No. 0111070100
文摘Based on the information from forest resources distribution maps of Luoning County of 1983 and 1999, six indices were used to analyze spatial patterns and dynamics of forest landscapes of the typical region in the middle of the Yihe-Luohe river basin. These indices include patch number, mean patch area, fragment index, patch extension index, etc. The results showed that: (1) There was a rapid increase in the number of patch and total area from 1983 to 1999 in the study area. The fragment degree became very high. (2) The area of all the forest patch types had witnessed great changes. The fractal degree of each forest patch type became big from 1983 to 1999. The mean extension index of Robinia pseudoacacia forest, non-forest, shrub forest, sparse forest, and Quercus species forest increased rapidly, but that of economic forest became zero. The fractal dimension each showed that forest coverage has been promoted. (3) The changes of landscape patterns were different in different geomorphic regions. From 1983 to 1999 the vegetation cover area, the gross number and the density of patch, diversity and evenness of landscape were all reduced greatly in gullies and ravines, but the maximum area and the mean area of patch types were increased. In hilly region, both the forest cover area and the number of patch increased from 1983 to 1999, but the mean area of patch was reduced greatly. In mountain region, even though the area under forest canopy reduced from 1983 to 1999, the patch number was increased greatly, the mean area of all patch types was reduced, the extension index, diversity index and evenness index of landscape were all increased. Furthermore, because of different types of land use, human activity and terrain, the vegetation changes on northern and southern mountain slopes were different. According to these analyses, the main driving forces, such as the policies of management, market economy, influence of human activities etc. are brought out.
文摘Human extracellular superoxide dismutase(hEC-SOD) is a secreted tetrameric protein involved in the protection of a human body from oxygen free radicals. Its three-dimensional structure has not been confirmed. hEC-SOD couldn′t be expressed in E.coli. We constructed a hybrid enzyme, which comprises the N-terminal and C-terminal domains from hEC-SOD, fused it to human Cu,Zn-SOD. The hybrid enzyme is expressed successfully in E.coli. Further, we analyzed the expression of hEC-SOD in E.coli by mRNA differential displaying.
文摘To enhance the relative movement of domains, we inserted a random sequence of fifteen-peptide into the three domains of L-aspartase. By means of directed screening, the three isoforms of monomeric, dimmeric and tetrameric enzymes were obtained. Compared to the wild-type tetrameric L-asparease, these mutants remained 19.7%, 42.3%, and 92% of the enzyme activity, respectively. Moreover, the examination of enzyme properties revealed that their k_ cat and K_M changed in varying degrees, and the optimum pH shifted towards acidic pH, while the dependence of the activity of enzyme on Mg 2+ concentration and thermostability increased. Therefore this strategy provides a novel approach to directed evolution of enzymes.
