Tumor-associated carbohydrate antigens(TACAs) are attractive targets for vaccine development. In this context, we described a strategy combining artificial TACA and glycoengineering for cancer vaccine development. A 2...Tumor-associated carbohydrate antigens(TACAs) are attractive targets for vaccine development. In this context, we described a strategy combining artificial TACA and glycoengineering for cancer vaccine development. A 2,4-ditrophenyl(DNP)-modified GM3 intermediate was synthesized chemoenzymatically and conjugated to keyhole limpet hemocyanin(KLH), and the resulting bioconjugate was tested for its potential as a vaccine candidate. Mice immunological studies revealed that the DNP-modified GM3(GM3-NHDNP) analog elicited strong and rapid immune responses by recruiting anti-DNP antibodies to facilitate the targeted delivery of the vaccine construct to antigen processing cells(APCs). Moreover, the endogenously produced anti-DNP antibodies, together with the elicited antibodies against GM3-NHDNP, may synergistically promote tumor binding and cancer cell death when the cancer cell surfaces are glycoengineered to express the GM3-NHDNP antigen.展开更多
We propose a linear mapping relationship between the polarization of the fundamental mode and the cylindrical vector(CV)modes on the first-order Poincaresphere(FOPS)in fiber.The new method is based on the fourdimensio...We propose a linear mapping relationship between the polarization of the fundamental mode and the cylindrical vector(CV)modes on the first-order Poincaresphere(FOPS)in fiber.The new method is based on the fourdimensional complex Jones matrices in terms of the linearly polarized mode bases.With our theoretical model,an all-fiber approach to generate arbitrary CV beams on the FOPS is proposed theoretically and verified experimentally.In the experiment,through the combination of a mode converter and a two-segment cascaded few-mode fiber with fixed stresses,it is possible to generate all CV modes on the FOPS by only adjusting the polarization of the fundamental mode.The Stokes parameters of the output light are measured to verify our scheme,which shows good agreement with the theoretical prediction.The method may provide a convenient way to generate CV beams and evolve the polarization states in any path on the FOPS,which is expected to have potential applications in encoding information and quantum computation.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 21907038, 32000904)the Natural Science Foundation of Jiangsu Province (No. BK20200601, China)+4 种基金the National Postdoctoral Program for Innovative Talents of China (No. BX20200153)the Health and Family Planning Commission of Wuxi, China (No. Z202005)the Social Development Key Project of Jiangsu Province (No. BE2019632, China)partly supported by the 111 Project (No. 111-2-06, China)the National First-class Discipline Program of Food Science and Technology (No. JUFSTR20180101, China)。
文摘Tumor-associated carbohydrate antigens(TACAs) are attractive targets for vaccine development. In this context, we described a strategy combining artificial TACA and glycoengineering for cancer vaccine development. A 2,4-ditrophenyl(DNP)-modified GM3 intermediate was synthesized chemoenzymatically and conjugated to keyhole limpet hemocyanin(KLH), and the resulting bioconjugate was tested for its potential as a vaccine candidate. Mice immunological studies revealed that the DNP-modified GM3(GM3-NHDNP) analog elicited strong and rapid immune responses by recruiting anti-DNP antibodies to facilitate the targeted delivery of the vaccine construct to antigen processing cells(APCs). Moreover, the endogenously produced anti-DNP antibodies, together with the elicited antibodies against GM3-NHDNP, may synergistically promote tumor binding and cancer cell death when the cancer cell surfaces are glycoengineered to express the GM3-NHDNP antigen.
基金National Natural Science Foundation of China(61875019,61675034,61875020,61571067)Fund of State Key Laboratory of IPOC(BUPT)Fundamental Research Funds for the Central Universities.
文摘We propose a linear mapping relationship between the polarization of the fundamental mode and the cylindrical vector(CV)modes on the first-order Poincaresphere(FOPS)in fiber.The new method is based on the fourdimensional complex Jones matrices in terms of the linearly polarized mode bases.With our theoretical model,an all-fiber approach to generate arbitrary CV beams on the FOPS is proposed theoretically and verified experimentally.In the experiment,through the combination of a mode converter and a two-segment cascaded few-mode fiber with fixed stresses,it is possible to generate all CV modes on the FOPS by only adjusting the polarization of the fundamental mode.The Stokes parameters of the output light are measured to verify our scheme,which shows good agreement with the theoretical prediction.The method may provide a convenient way to generate CV beams and evolve the polarization states in any path on the FOPS,which is expected to have potential applications in encoding information and quantum computation.
