Linlin and Pierre-Yves是极富有创意的艺术家,在数字创意领域颇有建树。近年来,他们设计的创意产品在海内外享有盛誉。随着3D打印技术的不断发展和成熟,他们所设计的艺术作品也提高到了一个新的维度。这些产品的设计灵感来源于生活,并...Linlin and Pierre-Yves是极富有创意的艺术家,在数字创意领域颇有建树。近年来,他们设计的创意产品在海内外享有盛誉。随着3D打印技术的不断发展和成熟,他们所设计的艺术作品也提高到了一个新的维度。这些产品的设计灵感来源于生活,并且带有巴黎这座城市特有的浪漫艺术气息,毕竟两位设计师大部分的时间都是在巴黎度过的。展开更多
Objective: To investigate the differen t expression of three isozymes of nitric oxide synthase (NOS) in diabetic rat colo ns and the contribution to the colonic dysfunction. Methods: Sprague-Dawley (SD) rats were us...Objective: To investigate the differen t expression of three isozymes of nitric oxide synthase (NOS) in diabetic rat colo ns and the contribution to the colonic dysfunction. Methods: Sprague-Dawley (SD) rats were used in this experiment and diabetes were induc ed by streptozotocin (65 mg/kg, i.v.). Three isozymes of NOS (nNOS, iNOS and eNO S) expression in proximal and distal colon were measured in two weeks after diab etes induction using the methods of immunocytochemistry and semi-quantitative r everse transcription-polymerase chain reaction (RT-PCR). Results: Positive immunoreactivity for nNOS was found in intermuscular and submucous plexus neuronal cells, neither eNOS nor iNOS had been found in any layers of col on in the two groups. The expression of nNOS mRNA was significantly increased in diabetic colon than that in control rats as determined by RT-PCR. The eNOS mRN A level of diabetic colon was lower compared to thecontrol rats, while no expres sion of iNOS mRNA was found in the normal or diabetic rats. Conclusion: This report has demonstrated that nNOS increased and eNOS decreased in rat colon in the early stages of diabetes. NO production by the nNOS might play a key role in colonic dysfunction, as supported by raised nNOS mRNA and enzyme e xpression in the diabetic colon. Reduced eNOS activity might also contribute to colonic dysfunction in experimental diabetes.展开更多
文摘Linlin and Pierre-Yves是极富有创意的艺术家,在数字创意领域颇有建树。近年来,他们设计的创意产品在海内外享有盛誉。随着3D打印技术的不断发展和成熟,他们所设计的艺术作品也提高到了一个新的维度。这些产品的设计灵感来源于生活,并且带有巴黎这座城市特有的浪漫艺术气息,毕竟两位设计师大部分的时间都是在巴黎度过的。
文摘Objective: To investigate the differen t expression of three isozymes of nitric oxide synthase (NOS) in diabetic rat colo ns and the contribution to the colonic dysfunction. Methods: Sprague-Dawley (SD) rats were used in this experiment and diabetes were induc ed by streptozotocin (65 mg/kg, i.v.). Three isozymes of NOS (nNOS, iNOS and eNO S) expression in proximal and distal colon were measured in two weeks after diab etes induction using the methods of immunocytochemistry and semi-quantitative r everse transcription-polymerase chain reaction (RT-PCR). Results: Positive immunoreactivity for nNOS was found in intermuscular and submucous plexus neuronal cells, neither eNOS nor iNOS had been found in any layers of col on in the two groups. The expression of nNOS mRNA was significantly increased in diabetic colon than that in control rats as determined by RT-PCR. The eNOS mRN A level of diabetic colon was lower compared to thecontrol rats, while no expres sion of iNOS mRNA was found in the normal or diabetic rats. Conclusion: This report has demonstrated that nNOS increased and eNOS decreased in rat colon in the early stages of diabetes. NO production by the nNOS might play a key role in colonic dysfunction, as supported by raised nNOS mRNA and enzyme e xpression in the diabetic colon. Reduced eNOS activity might also contribute to colonic dysfunction in experimental diabetes.
